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UCSD Transgenic Mouse and Gene Targeting Core
 

Summary: UCSD Transgenic Mouse and
Gene Targeting Core
9500 Gilman Drive, MC 0687
La Jolla, CA 92093-0687
(858) 534-3178
Current as of 3/01 1
PREPARATION OF GENOMIC DNA FROM MOUSE
TAIL TISSUE
Reagents
Lysis Buffer: for 1 liter
for 10mM Tris 1.21 g
for 100mM NaCl 5.84 g
for 10mM EDTA 3.72 g
for 0.5% SDS 5.00 g
balance to 1 liter with ddH2O
-pH solution to 8.0
-filter sterilize (45 micron filter)
Proteinase K Solution: 10mg/ml
BRL catalog number 5530UA 100mg
to reconstitute one bottle add:

  

Source: Abagyan, Ruben - School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego

 

Collections: Biology and Medicine