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The Polarization of Fluorescence of DNA Stains Depends on the Incorporation Density of the
 

Summary: The Polarization of Fluorescence of DNA Stains
Depends on the Incorporation Density of the
Dye Molecules
Jeanne L. Uy,1
Charles L. Asbury,2
Timothy W. Petersen,3
* and Ger van den Engh3
1
Cell Sorting Facility, Department of Pathology, University of Washington, Seattle, Washington
2
Department of Biological Sciences, Stanford University, Stanford, California
3
The Institute for Systems Biology, Seattle, Washington
Received 22 October 2003; Revision Received 3 March 2004; Accepted 15 March 2004
Background: The fluorescence induced by polarized
light sources, such as the lasers that are used in flow
cytometry, is often polarized and anisotropic. In addition,
most optical detector systems are sensitive to the direc-
tion of polarization. These two factors influence the accu-
racy of fluorescence intensity measurements. The inten-

  

Source: Asbury, Chip - Department of Physiology and Biophysics, University of Washington at Seattle

 

Collections: Biology and Medicine