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Elucidation of IgH intronic enhancer functions via germ-line deletion

Summary: Elucidation of IgH intronic enhancer functions
via germ-line deletion
Thomas Perlot*
, Frederick W. Alt*
, Craig H. Bassing* , Heikyung Suh
, and Eric Pinaud*
*The CBR Institute for Biomedical Research, Inc., and Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115;
Howard Hughes Medical Institute, Children's Hospital, 1 Blackfan Circle, Boston, MA 02115; and University of Vienna,
Dr.-Karl-Lueger-Ring1, A-1010 Vienna, Austria
Contributed by Frederick W. Alt, August 22, 2005
Studies of chimeric mice demonstrated that the core Ig heavy chain
(IgH) intronic enhancer (iE ) functions in V(D)J and class switch
recombination at the IgH locus. To more fully evaluate the role of
this element in these and other processes, we generated mice
homozygous for germ-line mutations in which the core sequences
of iE (cE ) were either deleted (cE / mice) or replaced with a
pgk-NeoR cassette (cE N/N mice). The cE / mice had reduced B
cell numbers, in association with impaired D to JH and VH to DJH
rearrangement, whereas cE N/N mice had a complete block in IgH


Source: Alt,, Frederick - Immune Disease Institute, Harvard University


Collections: Biology and Medicine