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Characterization of MdMADS2, a Member of the SQUAMOSA Subfamily of Genes, in Apple1
 

Summary: Characterization of MdMADS2, a Member of the
SQUAMOSA Subfamily of Genes, in Apple1
Soon-Kee Sung, Gyung-Hee Yu, and Gynheung An*
Department of Life Science, Pohang University of Science and Technology, Pohang 790784, Republic of Korea
A MADS-box gene, MdMADS2, was isolated from the apple
(Malus domestica Borkh.) var Fuji and its developmental expres-
sion pattern was studied during flower development. MdMADS2
shares a high degree of amino acid sequence identity with the
SQUAMOSA subfamily of genes. RNA blot analysis showed that
MdMADS2 is transcribed through all stages of flower development,
and its transcription was seen in the four floral organs. RNA in situ
hybridization revealed that the MdMADS2 mRNA is expressed both
in the inflorescence meristem and in the floral meristem. The Md-
MADS2 transcript was detected at all stages of flower development.
Protein localization analysis showed that MdMADS2 protein was
excluded from the stamen and carpel primordia, in which a consid-
erable MdMADS2 mRNA signal was detected. This indicates that
posttanscriptional regulation may be involved in the MdMADS2-
mediated control of flower development. Transgenic tobacco ex-
pressing the MdMADS2 gene from the cauliflower mosaic virus 35S

  

Source: An, Gynheung - Department of Life Science, Pohang University of Science and Technology

 

Collections: Biotechnology; Biology and Medicine