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Sample search results for: a1 protease activity

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1 Breakthrough Technologies Activity Profiling of Papain-Like Cysteine Proteases
 

Summary:  serine, C1A cysteine and A1 aspartic protease families in Arabidopsis. Phytochemistry 65: 43­58 Beers EP... Breakthrough Technologies Activity Profiling of Papain-Like Cysteine Proteases in Plants1 Renier A... . However, activity of many proteins, such as ...

  

Source: Bogyo, Matthew - Department of Microbiology and Immunology, Stanford University

 

Collection: Biology and Medicine

 
2 DOI: 10.1002/cbic.201000442 Mechanisms of Macromolecular Protease Inhibitors
 

Summary:  and temporal regulation, zymogen activation and protease degradation, and through the inhibition of proteases... an- notated human protease inhibitors responsible for regulating the activity of the 612 known human... is achieved, the ratio of approximately one Figure 1. A) Diagram of a ...

  

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

 

Collection: Biology and Medicine

 
3 Product Contents ProTEV Protease
 

Summary:  M DTT V6051 1,000 units V605A V602A (1ml) P117B (250µl) V6052 10,000 units V605B V602B (8ml) P117C (1... ) that has been engineered to be more stable than native TEV protease for prolonged enzymatic activity (1... activity is obtained at pH 7.0 and 30°C, but ProTEV ...

  

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

 

Collection: Biotechnology ; Biology and Medicine

 
4 http://genomebiology.com/2002/3/4/reviews/3006.1 commentreviewsreportsdepositedresearchinteractionsinformationrefereedresearch
 

Summary:  , shown as stereo pairs. In the single-chain aspartic protease (b), the corresponding loops are labeled A1... activity at specific sites to produce mature, functional units. Active retroviral proteases are homodimers... of four struc- tural elements: a hairpin ...

  

Source: Wlodawer, Alexander - Macromolecular Crystallography Laboratory, National Cancer Institute

 

Collection: Chemistry ; Biology and Medicine

 
5 Increased Heterologous Protein Production in Aspergillus niger Fermentation through Extracellular Proteases Inhibition by
 

Summary:  . The extracellular protease activity of the cultures varied with the pellet size and decreased dramatically when... was obtained from an inoculum of 4 × 106 spores/mL. It resulted in a specific protease activity of 158 units/ L... indicate that this bioprocessing strategy can be effectively used to inhibit ...

  

Source: Gu, Tingyue - Department of Chemical and Biomolecular Engineering, Ohio University

 

Collection: Biotechnology ; Chemistry

 
6 Biochemical Engineering Journal 8 (2001) 187193 Enhanced heterologous protein production in Aspergillus niger through
 

Summary:  in Aspergillus niger through pH control of extracellular protease activity Dara O'Donnella, Liping Wanga... 2000; accepted 15 February 2001 Abstract The extracellular protease activity of Aspergillus niger AB4... was designed to minimize protease activity and ...

  

Source: Gu, Tingyue - Department of Chemical and Biomolecular Engineering, Ohio University

 

Collection: Biotechnology ; Chemistry

 
7 I06 Biochemistry 1991, 30, 106-1 11 Wallace, C. J. A. (1987) J. Biol. Chem. 262, 16767-16770.
 

Summary:  by an anion-exchange column (Figure 4A1), while the HIV2 protease binds tightly and elutes with 1 M Na... Verlag, Basel. Inhibition of HIV Protease Activity by Heterodimer Formation? Lilia M. BabC, Sergio... , respectively. Incubating HIVl and H1V2 proteases at a 1 5 ...

  

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

 

Collection: Biology and Medicine

 
8 r XXXX American Chemical Society A dx.doi.org/10.1021/ja109377p |J. Am. Chem. Soc. XXXX, XXX, 000000 COMMUNICATION
 

Summary:  ­000 COMMUNICATION pubs.acs.org/JACS HIV Protease-Mediated Activation of Sterically Capped Proteasome Inhibitors... to exploit cleavage by HIV-1 protease to activate "Trojan horses" that selectively kill HIV-infected cells... modi ed to contain an HIV-1 protease recognition sequence; ...

  

Source: Crews, Craig M. - Department of Molecular Biophysics and Biochemistry, Yale University

 

Collection: Chemistry ; Biology and Medicine

 
9 Journal of Clinical Immunology, Vol. 18, No. 3, 1998 Special Article
 

Summary:  the inter-a-trypsin inhibitor (123, 124) and a1-macroglobulin (125) families of protease inhibitors... ). Newly synthesized proteases are targeted to and then activated in these organelles by a serglycin... active protease is targeted to the ...

  

Source: Sali, Andrej - Department of Biochemistry and Biophysics, University of California at San Francisco

 

Collection: Biology and Medicine ; Biotechnology

 
10 The S8 serine, C1A cysteine and A1 aspartic protease families in Arabidopsis
 

Summary:  cell death. Activation tagging of an as yet unidentified Ara- bidopsis A1 protease led to enhanced... The S8 serine, C1A cysteine and A1 aspartic protease families in Arabidopsis Eric P. Beersa... proteases (family ...

  

Source: Jones, Alan M. - Department of Biology, University of North Carolina at Chapel Hill

 

Collection: Biology and Medicine

 
11 Identification of Structural Mechanisms of HIV-1 Protease Specificity Using Computational Peptide
 

Summary:  noncleavable peptides with six protease active-site residues playing the most significant role... of the ten endogenous substrates in complex with a de-activated HIV-1 protease and proposed the substrate... ). They observed that all six substrate peptides con- formed to a common volume within the ...

  

Source: Gray, Jeffrey J. - Department of Biomolecular and Chemical Engineering, Johns Hopkins University

 

Collection: Chemistry ; Biology and Medicine

 
12 A Protease Activity Displaying Some Thrombin-like Characteristics in
 

Summary:  , Jones AM, Dickerman A. 2004. The S8 serine, C1A cysteine, and A1 aspartic protease families... A Protease Activity Displaying Some Thrombin-like Characteristics in Conditioned Medium of Zinnia... H optimum of 5.5­6.0 and displays some thrombin characteristics. This ...

  

Source: Jones, Alan M. - Department of Biology, University of North Carolina at Chapel Hill

 

Collection: Biology and Medicine

 
13 SUMO Technology Fusion Tag Protein Expression System
 

Summary:  fold correctly · Easy Purification ­ Both the tag and the protease have 6xHis tags · Native/Active... Protein ­ SUMO Protease leaves no unwanted residues on the N-terminus #12;SUMO is a Universal Tag PCR... of a library Directional cloning into vectors Expression and purification by Ni-NTA Cleavage to yield ...

  

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

 

Collection: Biotechnology ; Biology and Medicine

 
14 Hsuanyi Wu BI 690 Abstract
 

Summary:  numbers of different types of proteases. The hypothesis of this research is that a complement... of proteases is responsible for the degradation of proteins in the environment providing nutrients... . cinereus grows, and the different proteases make different quantitative and qualitative contribution

  

Source: Judd, Timothy M. - Department of Biology, Southeast Missouri State University

 

Collection: Environmental Sciences and Ecology ; Biology and Medicine

 
15 2011NatureAmerica,Inc.Allrightsreserved. nature structural & molecular biology advance online publication
 

Summary:  aspartates. The architecture of the active site in XMRV protease, particularly the hydrophobiclining... .6 and 23.2%, respectively. The final model includes residues 1-55 and 60-124 in monomer A, 1-5, 12... publication b r i e F com mu n i cat i on s Crystal structure of XMRV protease ...

  

Source: Wlodawer, Alexander - Macromolecular Crystallography Laboratory, National Cancer Institute

 

Collection: Chemistry ; Biology and Medicine

 
16 Regulatory Elements within the Prodomain of Falcipain-2, a Cysteine Protease of the Malaria Parasite
 

Summary:  an insertion relative to cathepsin L and cathepsin K. The a1 helix does not appear to actively inhibit... proteases, as the mediator of prodomain inhibitory activity. Circular dichroism analysis showed... activity, and these proteases are the ...

  

Source: Sali, Andrej - Department of Biochemistry and Biophysics, University of California at San Francisco

 

Collection: Biology and Medicine ; Biotechnology

 
17 10.1110/ps.035436.108Access the most recent version at doi: 2008 17: 2023-2037; originally published online Sep 29, 2008;Protein Sci.
 

Summary:  Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6... ; active sites; structure/function studies; protein turnover; structure; serine proteases; threonine... . In this work, we describe serine proteases with non- canonical ...

  

Source: Paetzel, Mark - Department of Molecular Biology and Biochemistry, Simon Fraser University

 

Collection: Biology and Medicine

 
18 2003 NaturePublishing Group Proteases perform essential functions in all living
 

Summary:  ) that is located in the active site from HUMAN AND MOUSE PROTEASES: A COMPARATIVE GENOMIC APPROACH Xose S. Puente... and 9A1 have also generated two copies of genes that encode the single- copy human metalloproteases ADAM... or conformational change of the prodomain,the protease becomes ...

  

Source: Overall, Christopher M. - Department of Oral Biological and Medical Sciences, University of British Columbia

 

Collection: Biology and Medicine

 
19 G-Biosciences 1-800-628-7730 www.GBiosciences.com A handbook & selection guide for
 

Summary:  ArrestTM shows excellent inhibition of protease activities and is therefore suitable for the protection... 95% of protease activities (e.g. 0.5mg/ml Mouse Pancreas Extract). The ProteaseArrestTM protease... . ProteaseArrestTM cocktails are used for ...

  

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

 

Collection: Biotechnology ; Biology and Medicine

 
20 The expanding diversity of serine hydrolases Istvan Botos1
 

Summary:  IgA1-specific serine peptidase S06.003 1WXR [16 ] NS3 protease S7 Flavivirin S07.001 2FOM,2FP7 [17... protease GlpG (2ic8). The core of the GlpG molecule is formed by six transmembrane helices (a1­a6... -linked homodimer [10] and cleaves factor IX. Each monomer has ...

  

Source: Wlodawer, Alexander - Macromolecular Crystallography Laboratory, National Cancer Institute

 

Collection: Chemistry ; Biology and Medicine


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21 Isatin Compounds as Noncovalent SARS Coronavirus 3C-like Protease Inhibitors
 

Summary:  -conjugated structures, some molecules might make a covalent bond with Cys145 at the active site of SARS CoV 3CL protease... -like protease with IC50 values below the micromolar level. The active site of SARS CoV 3CL protease contains Cys... active compound is an ...

  

Source: Luhua, Lai - Institute of Physical Chemistry, Peking University

 

Collection: Chemistry ; Biotechnology

 
22 A Phytophthora infestans Cystatin-Like Protein Targets a Novel Tomato Papain-Like Apoplastic Protease1[W][OA]
 

Summary:  C1A-1 to C1A-8. To in- vestigate the relationship between PIP1 and other Cys proteases of plants, we... using caspase inhibitors pointed to a role of plant proteases with caspase-like activity in the HR (D... vacuolar processing enzymes (VPEs) were recently identified to be Cys ...

  

Source: van der Knaap, Esther - Department of Horticulture and Crop Science, Ohio State University

 

Collection: Biology and Medicine

 
23 Crystal Structure of a Novel Viral Protease with a Serine/Lysine Catalytic Dyad Mechanism
 

Summary:  are essential for activity (Figure 4). Ser692 resides just before helix a1 and Lys729 resides within helix a2... , Canada V5A 1S6 2 Unite´ de Virologie et Immunologie Mole´culaires Institut National de la Recherche... a polyprotein (NH2-pVP2-X-VP4-VP3-COOH) that is processed through the ...

  

Source: Paetzel, Mark - Department of Molecular Biology and Biochemistry, Simon Fraser University

 

Collection: Biology and Medicine

 
24 Biochem. J. (2002) 368, 273281 (Printed in Great Britain) 273 Critical role of amino acid 23 in mediating activity and specificity of
 

Summary:  in mediating activity and specificity of vinckepain-2, a papain-family cysteine protease of rodent malaria... was expressed in Escherichia coli, solubilized, refolded and autoprocessed to an active enzyme. The protease... primers (A1, 5h-CCAATATGCTATTAGAAAAAATC- 3h ...

  

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

 

Collection: Biology and Medicine

 
25 10.1110/ps.052069306Access the most recent version at doi: 2006 15: 1815-1828Protein Sci.
 

Summary:  of the active site residues in several isolated Lon protease domains point to possible roles for the AAA... ), or, indeed, to any other proteases. Active-site-directed inhibitors of classical serine proteases... ), established that the active sites of Lon ...

  

Source: Wlodawer, Alexander - Macromolecular Crystallography Laboratory, National Cancer Institute

 

Collection: Chemistry ; Biology and Medicine

 
26 Binding and Cleavage of E. coli HUb by the E. coli Lon Protease Jiahn-Haur Liao,6
 

Summary:  reproducibility. Substrate interference assay Protease activity was measured as described previously (46... G13 and K18 is a loop that links the a1- and a2-helices. In the HUa monomer, the tertiary fold... are part of the preferred cleavage sites, are indicated. The ...

  

Source: Chiou, Arthur - Institute of Biophotonics, School of Biomedical Science and Engineering, National Yang-Ming University

 

Collection: Engineering ; Physics

 
27 Biol. Chem., Vol. 385, pp. 525535, June 2004 Copyright by Walter de Gruyter Berlin New York 2004/401100
 

Summary:  active site was estimated by their inhibitory effect on a1PI proteolytic degradation and DCG-04 binding... residual inhibitory activity. Enzyme was incubated with a1PI at enzyme:inhibitor molar ratio of 1... :100 for different time intervals. Inhibitory ...

  

Source: Bogyo, Matthew - Department of Microbiology and Immunology, Stanford University

 

Collection: Biology and Medicine

 
28 Biochem. J. (2004) 378, 529538 (Printed in Great Britain) 529 Identification and biochemical characterization of vivapains, cysteine
 

Summary:  -first-order equation y = A[1 - exp(- kobst) + B], where y is the fluorescence at time t, A the amplitude of the reac... .0). The refolded proteases were processed to enzymically active species of the sizes predicted for the mature... -Leu-Arg-AMC, consistent with activities of other ...

  

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

 

Collection: Biology and Medicine

 
29 Protocol 20. Factor Xa Protease treatment of fusion proteins containing a Factor Xa Protease recognition sequence
 

Summary:  , because Factor Xa Protease activity is sensitive to phosphate buffers as well as to high imidazole and Na... the aliquots with SDS-PAGE sample buffer immediately to completely quench Factor Xa Protease activity. 5... to be cleaved: Factor Xa Protease activity is ...

  

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

 

Collection: Biotechnology ; Biology and Medicine

 
30 Chem. Eng. Comm., 196:481490, 2009 Copyright 0Taylor & Francis Group, LLC
 

Summary:  , theprotease activity could be kept low most of the time during thefermentation. The increase of protease... H control (O'Donnell et al., 2001) to inhibit extracellular protease activity and thereby increase heterolo... ., 1987) and N402 is a cspA1 derivative of strain ...

  

Source: Gu, Tingyue - Department of Chemical and Biomolecular Engineering, Ohio University

 

Collection: Biotechnology ; Chemistry

 
31 Protein Expression and Purification 24, 6170 (2002) doi:10.1006/prep.2001.1545, available online at http://www.idealibrary.com on
 

Summary:  A, gene encoding tmRNA in E. coli; LB, Luria­Bertani; DIG, digoxigenin; EOP, equivalent to a1 To whom... Codons, Limits the Accumulation of Recombinant Tobacco Etch Virus Protease in Escherichia coli BL21(DE3... protease enteropeptidase (enterokinase), or thrombin. However, from tobacco etch virus (TEV ...

  

Source: Wlodawer, Alexander - Macromolecular Crystallography Laboratory, National Cancer Institute

 

Collection: Chemistry ; Biology and Medicine

 
32 SUMO Protease 1 Size: 1000 units
 

Summary:  SUMO Protease 1 Size: 1000 units Description and Application SUMO Protease, a highly active... and robust recombinant protease, cleaves SUMO (Small Ubiquitin-like MOdifier) from recombinant fusion... proteins. Unlike thrombin, EK, or TEV protease, whose recognition sequences are short and ...

  

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

 

Collection: Biotechnology ; Biology and Medicine

 
33 SUMO Protease 2 Size: 1000 units
 

Summary:  SUMO Protease 2 Size: 1000 units Description and Application SUMO Protease 2, a highly active... and robust recombinant protease, cleaves SUMO (Small Ubiquitin-like MOdifier) from recombinant fusion... proteins. Unlike thrombin, EK, or TEV protease, whose recognition sequences are short ...

  

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

 

Collection: Biotechnology ; Biology and Medicine

 
34 nature structural & molecular biology VOLUME 18 NUMBER 2 FEBRUARY 2011 227 b r i e F com m u n i cat i o n s
 

Summary:  aspartates. The architecture of the active site in XMRV protease, particularly the hydrophobiclining... cat i o n s Crystal structure of XMRV protease differs from the structures of other retropepsins Mi Li... determined the crystal structure of the protease encoded by xenotropic murine leukemia ...

  

Source: Baker, David - Center for Nanotechnology and NanoTechnology & Department of Biochemistry, University of Washington at Seattle; Wlodawer, Alexander - Macromolecular Crystallography Laboratory, National Cancer Institute

 

Collection: Biology and Medicine ; Biotechnology ; Chemistry

 
35 NATURE BIOTECHNOLOGY VOL 18 AUGUST 2000 http://biotech.nature.com 847 Interactions between proteins play a critical role in cell function and
 

Summary:  protease. A1 through E8 are protease-coated surfaces to which 40 lysates were applied. Binding was detected... -dena- tured or native protease on the surface of a maleimide- activated 96-well plate through its two reactive... -affinity interactions with the ...

  

Source: Park, Sang-Hyun - School of Biological Sciences, Seoul National University; Raines, Ronald T. - Departments of Biochemistry & Chemistry, University of Wisconsin at Madison

 

Collection: Biology and Medicine ; Chemistry

 
36 Journal of Chemical Technology and Biotechnology J Chem Technol Biotechnol 78:12591266 (online: 2003) DOI: 10.1002/jctb.898
 

Summary:  most significantly. Higher agitation rate resulted in higher GFP production. Protease activity was most... interleukin-6, bovine chymosin, and porcine pancreatic phospholi- pase A.1,2 A fungal fermentation system... by the host. The strain AB4.1 is a pyrG1 derivative of N402 3 and N402 is a ...

  

Source: Gu, Tingyue - Department of Chemical and Biomolecular Engineering, Ohio University

 

Collection: Biotechnology ; Chemistry

 
37 letters to nature NATURE |VOL 407 |19 OCTOBER 2000 |www.nature.com 923
 

Summary:  `activation' salt-bridge between the N-terminal amine and Asp 194 of trypsin with Lys 328 of a1-antitrypsin. d... of the active serine of the protease with the reactive centre of the serpin. This cleaves the reactive centre... activation4 . The disruption of the ...

  

Source: Read, Randy J. - Department of Haematology, University of Cambridge

 

Collection: Biology and Medicine

 
38 pubs.acs.org/BiochemistryPublished on Web 05/12/2009r 2009 American Chemical Society Biochemistry 2009, 48, 57535759 5753
 

Summary:  University, South Science Building, 8888, University Drive, Burnaby, British Columbia, Canada V5A 1S6... proteinfragmentsgenerated bythe addition of a protease or activation of a protease pathway (15, 16). Also, information about... substrates of proteases can be ...

  

Source: Paetzel, Mark - Department of Molecular Biology and Biochemistry, Simon Fraser University

 

Collection: Biology and Medicine

 
39 HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Urokinase-type plasminogen activator is a preferred substrate of the human
 

Summary:  1AT Neutrophil elastase and TMT 0 95 SLPI Varied serine proteases 15 90 The protease activity... - ficity. Once activated, tryptase cannot be inhibited effectively by the diverse array of protease... active protease. Tryptase ...

  

Source: Sali, Andrej - Department of Biochemistry and Biophysics, University of California at San Francisco

 

Collection: Biology and Medicine ; Biotechnology

 
40 Our view of the proteolytic universe has expanded con-siderably in recent years.PROTEASES were initially charac-
 

Summary:  cell surfaces; activation or inactivation of proteases and other enzymes,cytokines,hormones or growth... protease activity in physiological and pathological conditions.Hence,the emerging pattern... .Specific activity and redundancy are crucial determinants of the role of a particular ...

  

Source: Overall, Christopher M. - Department of Oral Biological and Medical Sciences, University of British Columbia

 

Collection: Biology and Medicine


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41 Protease Assay Kit TB252 11/99 Novagen 1
 

Summary:  when processing animal or plant cells or cells of micro-organisms. Protease activity can affect... activity of the dehydrogenase. The assay detects proteases with an optimal pH in the neutral to alkaline... of protease activity, but the residual dehydrogenase ...

  

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

 

Collection: Biotechnology ; Biology and Medicine

 
42 EnzChekTM Protease Assay Kits One-step fluorescence-based protease assays
 

Summary:  , is pro- portional to protease activity (Figure 2). BODIPY FL and BODIPY TR-X casein can be used... EnzChekTM Protease Assay Kits One-step fluorescence-based protease assays ® Easy Simply incubate... H insensitive. Use to assay a wide variety of proteases. Convenient Use a fluorescence ...

  

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

 

Collection: Biotechnology ; Biology and Medicine

 
43 An overview of enzymatic reagents for the removal of affinity tags David S. Waugh
 

Summary:  of various reagents. Endoproteases For many years, serine proteases such as activated blood coag- ulation... to autoinactivation than the wild-type protease and, fortuitously, also has moderately greater catalytic activity [18... .1 and 2.0 M. In a later study, TEV protease ...

  

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

 

Collection: Biotechnology ; Biology and Medicine

 
44 The variable detergent sensitivity of proteases that are utilized for recombinant protein affinity tag removal
 

Summary:  other enzymes, the activities of proteases can be affected by buffer conditions. The buffers used... activity of viral proteases is reflected, empirically, in the obser- vation that those labs which utilize... gold stain. Results and discussion In order to assess the activity of commonly ...

  

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

 

Collection: Biotechnology ; Biology and Medicine

 
45 Comparison of the substrate specificity of two potyvirus Jo zsef To zser1
 

Summary:  model of TVMV NIa protease (magenta) on the crystal structure of the C151A active site mutant TEV NIa... a predominantly TEV protease-like activity, residues of the TVMV substrates are considered here as mutants... with a homology model of TVMV protease suggested that the major ...

  

Source: Wlodawer, Alexander - Macromolecular Crystallography Laboratory, National Cancer Institute

 

Collection: Chemistry ; Biology and Medicine

 
46 Protease Detection Kit Contamination Control Kits
 

Summary:  derivative as protease substrate, to be incubated with the sample for 2 to 20 hours at 37°C. Active protease... in the supernatant at 492 nm. The intensity of absorbance is proportional to the total protease activity... already allows a visual estimate of protease ...

  

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

 

Collection: Biotechnology ; Biology and Medicine

 
47 Proc. Natl. Acad. Sci. USA Vol. 94, pp. 55825587, May 1997
 

Summary:  and protease B inhibitor 2 (I2 B ). Here we show that the second low molecular weight activity (LMA2... inhibitor activity is not related to its ability to promote vacuole fusion: (i) Low Mr protease B inhibitors... 2 B in promoting vacuole fusion and far less active in inhibiting ...

  

Source: Haas, Albert - Institute for Cell Biology, Universität Bonn

 

Collection: Biology and Medicine

 
48 Protein Expression and PuriWcation 38 (2004) 108115 www.elsevier.com/locate/yprep
 

Summary:  was very similar to that of TEV protease. Its catalytic activity was greatest in the absence of Na... . The catalytic eYciency and dependence of TVMV and TEV protease activities on monovalent salt concentration... -terminus, yielding a truncated enzyme with greatly diminished activity ...

  

Source: Wlodawer, Alexander - Macromolecular Crystallography Laboratory, National Cancer Institute

 

Collection: Chemistry ; Biology and Medicine

 
49 SUMO Protease Cat. No. 12588-018 Size: 250 units
 

Summary:  SUMO Protease Cat. No. 12588-018 Size: 250 units Description SUMO Protease, a highly active... affect the activity of the protease. Recommended Conditions for Cleavage of a Fusion Protein An example... > 85% single-band purity and must demonstrate functional absence of any non-specific ...

  

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

 

Collection: Biotechnology ; Biology and Medicine

 
50 Pseudomonas aeruginosa protease IV degrades surfactant proteins and inhibits surfactant host defense and biophysical functions.
 

Summary:  -staining. Colorimetric Substrate Assay for Protease IV Activity Protease IV activity was determined by the hydrolysis... 'Callaghan et al. (28). Protease IV activity was measured as an increase in optical density at 410 nm. End... of protease IV ...

  

Source: Hogan, Brigid L.M. - Department of Cell Biology, Duke University; Wright, Jo Rae - Department of Cell Biology, Duke University

 

Collection: Biology and Medicine

 
51 Viral Proteases Department of Biological Sciences, Columbia University, New York, New York 10027
 

Summary:  of the Proteases 4615 B. Active Sites of the Proteases 4616 C. Inhibitors of the Proteases 4616 IV. Papain... for Catalytic Activity 4621 VII. Adenovirus Protease, a Novel Cysteine Protease 4621 A. The Protease... of ...

  

Source: Tong, Liang - Department of Biological Sciences, Columbia University

 

Collection: Biology and Medicine

 
52 [M189]. Mirabilysin MEROPS name: Mirabilysin[M189]P001
 

Summary:  to as an IgA protease because of its activity on both serum and secretory forms of IgA1 (immunoglobulin A1... mirabilysin. Both human serum IgA1 and human IgG were digested by the recombinant metal- loprotease... . Recombinant ...

  

Source: Belas, M. Robert - Center of Marine Biotechnology, University of Maryland Biotechnology Institute

 

Collection: Biology and Medicine

 
53 Structural determinants of tobacco vein mottling virus protease substrate
 

Summary:  the disordered residues from TEV protease reduces its catalytic activity by ~10-fold, an analogous truncation... mutant of TVMV protease is significantly more active. Comparison of the structures of TEV and TVMV... in the active site. TVMV protease shares 52% amino ...

  

Source: Waugh, David S.- Macromolecular Crystallography Laboratory, National Cancer Institute

 

Collection: Biology and Medicine

 
54 Chemistry & Biology Peptide Length and Leaving-Group Sterics Influence
 

Summary:  in a live-cell labeling application that specifically targets active S1A proteases at the cell surface... functional analyses of individual caspases during apoptosis. However, covalent labeling of active proteases... ­57, January 28, 2011 ª2011 Elsevier Ltd All rights reserved #12;monitor ...

  

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

 

Collection: Biology and Medicine

 
55 A ribonuclease zymogen activated by the NS3 protease of the hepatitis C virus
 

Summary:  A ribonuclease zymogen activated by the NS3 protease of the hepatitis C virus R. J. Johnson1... [3]. After processing of a zymogen to a mature protease, a cell can restrict proteolytic activity... activity of ribonucleases [7,8], which, like proteases, can degrade an ...

  

Source: Raines, Ronald T. - Departments of Biochemistry & Chemistry, University of Wisconsin at Madison

 

Collection: Chemistry ; Biology and Medicine

 
56 MOLECULAR AND CELLULAR BIOLOGY, 0270-7306/00/$04.00 0
 

Summary:  with MurA, and a clone in which 2A protease activity is efficiently induced was selected as described... R-2A) mRNA (Fig. 2A). The cleavage products noted following the activation of the 2A protease were... monitored the effect of induced 2A protease on protein synthesis ...

  

Source: Kahana, Chaim - Department of Molecular Genetics, Weizmann Institute of Science

 

Collection: Biology and Medicine

 
57 Branden & Tooze, Chapter 11 An enzyme that hydrolyzes the peptide bond
 

Summary:  developmental process and lifecycle of pathogens (virus and parasite) both depend on protease activity ­ cancer... induces dimerization and activation Picornain ­ structurally similar to serine protease chymotrypsin... but active site ser has been mutated to cys ­ important for the ...

  

Source: Park, Sheldon - Department of Chemical and Biological Engineering, State University of New York at Buffalo

 

Collection: Biology and Medicine ; Biotechnology

 
58 This article was published in an Elsevier journal. The attached copy is furnished to the author for non-commercial research and
 

Summary:  ://www.elsevier.com/copyright #12;Author's personal copy Substrate Modulation of Enzyme Activity in the Herpesvirus Protease Family... -protein interactions; X-ray structure Introduction The herpesvirus family encodes a protease whose activity... ­11 Concentration depen- dent dimerization within the nuclear ...

  

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

 

Collection: Biology and Medicine

 
59 Genes encoding proteases within any genome are easily identified, and three-dimensional
 

Summary:  of active proteases3. The new probes enable imaging proteases in real time and at the point of their action... proteases in action Klaudia Brix & Silvia Jordans Activity-based probes can be used for monitoring enzyme... now been developed that becomes fluorescent only after labeling ...

  

Source: Bogyo, Matthew - Department of Microbiology and Immunology, Stanford University

 

Collection: Biology and Medicine

 
60 treatment are self-regulatory, through the activation of lymphocytes, which inhibit the activity of
 

Summary:  or threonine proteases or an activated water molecule in the metallo or aspartyl proteases, acts... . Aberrant regulation of endogenous protease activity results in a variety of life-threatening illnesses... in proteases can complicate the situation further, as seen ...

  

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

 

Collection: Biology and Medicine


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61 Science Highlight June 2010 Figure 1. Surface rendering of the HIV protease
 

Summary:  in pockets and clefts on the protease surface and not in the active site. Computer simulations show that one... sites exist in HIV protease outside the active site and establish a starting point for developing larger... Science Highlight ­ June 2010 Figure 1. Surface rendering of the HIV ...

  

Source: Wechsler, Risa H. - Kavli Institute for Particle Astrophysics and Cosmology, Stanford Linear Accelerator Center

 

Collection: Physics

 
62 Structural and biochemical studies of retroviral proteases Alexander Wlodawer *, Alla Gustchina
 

Summary:  proteases, such as inhibition by pepstatin [14^16] and inactivation by mutating the putative active site... : a hairpin (containing loops A1 and A2), a wide loop (B1, containing the catalytic aspartic acid, and B2... , and is replaced by a loop in HIV (both types 1 and 2) and SIV PRs. Also, the length of loops ...

  

Source: Wlodawer, Alexander - Macromolecular Crystallography Laboratory, National Cancer Institute

 

Collection: Chemistry ; Biology and Medicine

 
63 INSTRUCTIONS Pierce Biotechnology PO Box 117 (815) 968-0747 www.thermo.com/pierce
 

Summary:  activity Sample contains high levels of proteases or phosphatases Add sufficient cocktail to produce a 2X... Road Rockford, lL 61105 USA (815) 968-7316 fax Number Description 78440 Halt Protease and Phosphatase... Inhibitor Cocktail, sufficient for 100 ml of sample Contents: Protease and Phosphatase ...

  

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

 

Collection: Biotechnology ; Biology and Medicine

 
64 Structure of the Archaeal Pab87 Peptidase Reveals a Novel Self-Compartmentalizing Protease Family
 

Summary:  -compartmentalizing proteases among living organisms [11]. Pab87 exopeptidase activities As Pab87 is a S12 protease, the amino... of activity was obtained at 90uC, the Figure 1. Primary structure of the CubicO proteases. Schematic... and refinement statistics. Lu-HPDO3A derivative Data ...

  

Source: Ecole Polytechnique, Centre de mathématiques

 

Collection: Mathematics

 
65 SUMOstar Protease 1 Size: 1000 units
 

Summary:  SUMOstar Protease 1 Size: 1000 units Description and Application SUMOstar Protease, a highly active... and robust recombinant protease, cleaves SUMOstar from recombinant fusion proteins. Unlike thrombin, EK... , or TEV protease, whose recognition sequences are short and degenerate, ...

  

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

 

Collection: Biotechnology ; Biology and Medicine

 
66 Stage-specific profiling of Plasmodium falciparum proteases using an internally quenched multispecificity protease substrate
 

Summary:  protease substrate was used to profile the proteolytic activities in the malarial parasite Plasmodium... . Cysteine protease activity was shown to be dominating at neutral pH, whereas aspartic protease activity... for protease ...

  

Source: Balaram , P. - Molecular Biophysics Unit, Indian Institute of Science, Bangalore

 

Collection: Biology and Medicine

 
67 Escherichia coli Signal Peptide Peptidase A Is a Serine-Lysine Protease with a Lysine Recruited to the Nonconserved Amino-Terminal Domain in the S49 Protease
 

Summary:  , Simon Fraser UniVersity, Burnaby, British Columbia V5A 1S6, Canada, The Skaggs Institute for Chemical... in the carboxyl-terminal protease domain (residues 326-549) is critical for activity, suggesting this domain lacks... . They showed that Ser 162 and Lys 214 were critical for ...

  

Source: Paetzel, Mark - Department of Molecular Biology and Biochemistry, Simon Fraser University

 

Collection: Biology and Medicine

 
68 Escherichia coli Signal Peptide Peptidase A Is a Serine-Lysine Protease with a Lysine Recruited to the Nonconserved Amino-Terminal Domain in the S49 Protease
 

Summary:  , Simon Fraser UniVersity, Burnaby, British Columbia V5A 1S6, Canada, The Skaggs Institute for Chemical... in the carboxyl-terminal protease domain (residues 326-549) is critical for activity, suggesting this domain lacks... . They showed that Ser 162 and Lys 214 were critical for ...

  

Source: Cravatt, Benjamin - Department of Cell Biology, Scripps Research Institute

 

Collection: Biology and Medicine

 
69 Name______________________________________ Exam 2 4 pages... all answers must fit in space provided! Matching (20 pts). Place the letter of the best
 

Summary:  activity decreases over a period of about 1-2 hours. The inactivation of esterase 2a1 by compound M... pages... all answers must fit in space provided! 2 12. (10 pts) The enzyme "esterase 2a1" catalyzes... the reaction shown below. Compound M causes time-dependent inactivation of ...

  

Source: Gates, Kent. S. - Departments of Chemistry & Biochemistry, University of Missouri-Columbia

 

Collection: Chemistry

 
70 The 0.83 A Resolution Crystal Structure of a-Lytic Protease Reveals the Detailed Structure of the Active
 

Summary:  A. Data collection Resolution range (A° ) 1.7­0.78 1-1.12 Detector distance (mm) 99 200 MAR plate... The 0.83 A° Resolution Crystal Structure of a-Lytic Protease Reveals the Detailed Structure... of the Active Site and Identifies a Source of Conformational Strain This paper is dedicated to Dr Peter A

  

Source: Agard, David - Department of Biochemistry and Biophysics, University of California at San Francisco

 

Collection: Biotechnology ; Biology and Medicine

 
71 A fluorescence quenching assay to discriminate between specific and nonspecific inhibitors of dengue virus protease
 

Summary:  , Amedeo Caflisch c , Xun-Cheng Su d , Kiyoshi Ozawa d , Gottfried Otting d , Subhash G. Vasudevan a,1... the substrate Bz-nKRR-AMC for monitoring dengue single-chain NS2B/NS3 protease activity [22]. The assay was used... because it did not inhibit the protease ...

  

Source: Caflisch, Amedeo - Department of Biochemistry, Universität Zürich

 

Collection: Biology and Medicine

 
72 A Model of Tumour Angiogenesis and
 

Summary:  the actions of the protease. We will investigate whether this mechanism can curtail angiogenic activity. 5... that is in the intermediate #12;126 complex (CAF). Angiostatin (A) combines with active protease to form inactive pro- tease... e = k7 k-7 ) and hence [CA] = [C] - [CAF] 1 + e ...

  

Source: Plank, Mike - Department of Mathematics and Statistics, University of Canterbury

 

Collection: Mathematics

 
73 Developmental Cell 10, 4555, January, 2006 2006 Elsevier Inc. DOI 10.1016/j.devcel.2005.11.013 A Spatzle-Processing Enzyme Required for Toll
 

Summary:  , such as immunity and development, by recruiting similar ligand-processing proteases with distinct activation modes... Toll activation after im- mune challenge, the involvement of other protease(s) with Easter... ., 2002a). Thus, in the case of G+ bacteria, another protease cascade may be ...

  

Source: Lemaitre, Bruno - Centre de Génétique Moléculaire, CNRS

 

Collection: Biology and Medicine

 
74 Communication between the active sites and dimer interface of a herpesvirus protease revealed by a
 

Summary:  Communication between the active sites and dimer interface of a herpesvirus protease revealed... of the active site and the dimer interface of wild-type protease in solution. Positional scanning synthetic... concentration of protease is thought to drive dimerization of monomers, which ...

  

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

 

Collection: Biology and Medicine

 
75 Temperature Effects on Product-Quality-Related Enzymes in Batch CHO Cell Cultures Producing Recombinant tPA
 

Summary:  and with a constant temperature of 37 °C. Analysis of extracellular protease activity indicated that two major... proteases were present (50 and 69 kDa). The 50 kDa protease activity decreased similarly with time for both... culture conditions. The 69 kDa protease ...

  

Source: Tullos, Desiree - Department of Biological and Ecological Engineering, Oregon State University

 

Collection: Environmental Sciences and Ecology

 
76 Crystal Structure of the VP4 Protease from Infectious Pancreatic Necrosis Virus Reveals the Acyl-Enzyme Complex
 

Summary:  Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada and § Unite... the proteolytic activity of its own protease, VP4, to release the pro- teins pVP2 and VP3. pVP2 is further... , Simon Fraser University, South Science Bldg., 8888 Uni- versity Dr., Burnaby, British ...

  

Source: Paetzel, Mark - Department of Molecular Biology and Biochemistry, Simon Fraser University

 

Collection: Biology and Medicine

 
77 HIV protease inhibitors and nuclear lamin processing: Getting the right bells and whistles
 

Summary:  prelamin A. They also showed that HIV protease inhibitors interfered with the enzymatic activity of ZMPSTE... might the HIV protease inhibitors interfere with ZMPSTE24 protease activ- ity? As shown in Fig. 1... HIV protease inhibitors and nuclear lamin processing: ...

  

Source: Clarke, Steven - Department of Chemistry and Biochemistry, University of California at Los Angeles

 

Collection: Biology and Medicine

 
78 Computational Coevolution of Antiviral Drug Resistance Christopher D. Rosin 1;2 , Richard K. Belew 2 , Garrett M. Morris 1 ,
 

Summary:  takes place at a localized active site (central white area in Figure 1) in the protease. Protease... inhibitors bind to this active site, preventing the 2 #12; normal function of HIV­1 protease and interrupting... such an octapeptide (in green) bound in the active ...

  

Source: Belew, Richard K. - Departments of Computer Science and Engineering & Cognitive Science, University of California at San Diego

 

Collection: Computer Technologies and Information Sciences

 
79 Inhibition of R-Lytic Protease by Pro Region C-Terminal Steric Occlusion of the Active Site
 

Summary:  Inhibition of R-Lytic Protease by Pro Region C-Terminal Steric Occlusion of the Active Site Julie L... the protease active site. Instead, the catalytic site is fully functional in the complex. Pro region inhibition... and a large amino-terminal pro region which is not part of the active ...

  

Source: Agard, David - Department of Biochemistry and Biophysics, University of California at San Francisco; Rader, Stephen - Chemistry Program, University of Northern British Columbia

 

Collection: Biology and Medicine ; Biotechnology ; Chemistry

 
80 Two Proteases Defining a Melanization Cascade in the Immune System of Drosophila*
 

Summary:  proteases, MP1 and MP2. A, the null mutation spn27A1 for the serpin Spn27A results in constitutive... . Uninfected wild-type (wt) flies have only a low level of PO activity. Control spn27A1 homozygotes (spn27A... . In Drosophila, the serpin Spn27A regulates melaniza- ...

  

Source: Lemaitre, Bruno - Centre de Génétique Moléculaire, CNRS

 

Collection: Biology and Medicine


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81 Analysis of Methods for Predicting Protein Fold and Remote Homologue Recognition
 

Summary:  ). Upon selection, the method generates 1D-PSSM or 1D-profile using PSI- Blast (A0,A1,A2...). Next... , oligopeptidase and omega-peptidase activity. Serine proteases of the chymotrypsin family share a common fold... -ray structure has been determined (Rypniewski, et. al, 1993). Trypsin or serine ...

  

Source: Bejerano, Gill - Departments of Computer Science & Developmental Biology, Stanford University

 

Collection: Computer Technologies and Information Sciences ; Biology and Medicine

 
82 Hemoglobin Degradation in the Human Malaria Pathogen Plasmodium falciparum: A Catabolic
 

Summary:  of an endogenous aspartic protease to cleave intact hemoglobin before other proteolytic activities could function... for aspartic protease activity as described below. Activity eluted between 0.3 and 0.5 M. These fractions were... summarized in Table 3. At late time points the a34-141 ...

  

Source: Chait, Brian T. - Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, Rockefeller University

 

Collection: Biology and Medicine

 
83 Inhibition of tobacco etch virus protease activity by detergents Arun K. Mohanty, Chad R. Simmons, and Michael C. Wiener*
 

Summary:  of a polyhistidine affinity tag from the recombinant soluble protein a1 platelet activating factor acetylhydrolase... . Materials and methods Materials The plasmid pHis-PAFAHa1 encoding a1-platelet activating factor... concentration; TEV, tobacco etch virus; rTEV, ...

  

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

 

Collection: Biotechnology ; Biology and Medicine

 
84 RESEARCH ARTICLE Liberation of SARS-CoV main protease from the viral
 

Summary:  perform N-terminal autocleavage, while the dimerization of mature protease and trans- cleavage activity... dimers while the mutant still maintains a trans-cleavage activity comparable to that of WT protease. Thus... autocleavage activity comparable to WT protease. ...

  

Source: 

 

Collection: Computer Technologies and Information Sciences

 
85 Classification of ATP-dependent proteases Lon and comparison of the active sites of their proteolytic domains
 

Summary:  Classification of ATP-dependent proteases Lon and comparison of the active sites... the definition of two subfamilies of Lon proteases, LonA and LonB, based on the consensus sequences in the active... functional catalytic Ser­Lys dyad might also be present in the active site of Lon ...

  

Source: Wlodawer, Alexander - Macromolecular Crystallography Laboratory, National Cancer Institute

 

Collection: Chemistry ; Biology and Medicine

 
86 The Cortical Granule Serine Protease CGSP1 of the Sea Urchin, Strongylocentrotus purpuratus,
 

Summary:  and active forms of the protease, is detected in cortical granules by immunoblot analysis. Our evidence... suggests that CGSP1 is activated at fertilization and is responsible for the protease- mediated reactions... laevis (Lindsay and Hedrick, 1989), which appears to cleave and activate ...

  

Source: Wessel, Gary M. - Department of Molecular Biology, Cell Biology and Biochemistry, Brown University

 

Collection: Biology and Medicine

 
87 Proc. Natl. Acad. Sci. USA Vol. 93, pp. 11002-11007, October 1996
 

Summary:  17 and poly(ADP-ribose) polymerase is cleaved indicating that this protease although activated... to be converted from an inactive pro-form as a single inactive polypeptide chain to an active protease (5... another protease to activate it. Although it is not known if ...

  

Source: 

 

Collection: Biology and Medicine

 
88 CIAP1 and the serine protease HTRA2 are involved in a novel p53-dependent
 

Summary:  . HTRA2 requires an intrinsic serine protease activity for its proapoptotic function. Little is known... -terminal Reaper-like motif, which then acti- vates apoptosis via its intrinsic serine protease activity... caspases, certain serine proteases such as granzyme B have been reported to ...

  

Source: Chait, Brian T. - Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, Rockefeller University

 

Collection: Biology and Medicine

 
89 BIOINFORMATICS Vol. 19 no. 1 2003
 

Summary:  in the active site. This study also investigated the resistance of HIV protease mutants to Saquinavir (another... protease active site is located in a cleft into which the polypeptide to be cleaved is positioned... be stopped or drastically reduced if the activity of the ...

  

Source: Draghici, Sorin - Department of Computer Science, Wayne State University

 

Collection: Computer Technologies and Information Sciences

 
90 Craik, Charles Faculty Training History
 

Summary:  , CA DeCamp, D. Post 1988-1993 Elucidation of the active site of HIV proteases University of Delaware... -2002 Chemical control of serine protease activation Duke University, PhD 1997 Assoc. Professor, San Francisco... of Support Higaki, J. Post 1986-1990 Dissecting the active site of ...

  

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

 

Collection: Biology and Medicine

 
91 news and views nature structural biology volume 5 number 12 december 1998 1021
 

Summary:  remains in the folded, active native protease conformation (`N' state) after removal of the pro region... - hairpin may be an important part of the pro region's refolding activity. The -lytic protease pro region... binding interface into the protease active sites ...

  

Source: Baker, David - Center for Nanotechnology and NanoTechnology & Department of Biochemistry, University of Washington at Seattle

 

Collection: Chemistry ; Biotechnology

 
92 The cpmplete Guide for Protease Inhibition cPmplete protection... cPmplete convenience
 

Summary:  : High-efficiency protease inhibition a Contain serine and histidine in the active center b Contain... center d Contain aspartic (acidic) group in the active center Inhibits serine and cysteine proteases... for protection against unwanted protease activity in your ...

  

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

 

Collection: Biotechnology ; Biology and Medicine

 
93 Original Article Novel Antifoulants: Inhibition of Larval Attachment by Proteases
 

Summary:  showing a clear zone indicated which bacteria showed higher protease activity. Six bacterial strains (D1... -001, D2-005, D3- 103, D4-001, D12-004, and D12-006) exhibited the strongest protease activity and were... protease activity. A skim-milk agar plate containing ...

  

Source: Levin, Lisa - Integrative Oceanography Division, Scripps Institution of Oceanography

 

Collection: Environmental Sciences and Ecology

 
94 Regular paper Limited proteolysis of E. coli ATP-dependent protease Lon a unified
 

Summary:  protein substrate is itself an active protease. An enzyme that fulfills well the requirements outlined... of active LonA proteases is still unclear. Sedimentation studies of Lon protease from Mycobacterium... -w.t. or S679A (1­784) 87.4 ND ~ 1000 ...

  

Source: Wlodawer, Alexander - Macromolecular Crystallography Laboratory, National Cancer Institute

 

Collection: Chemistry ; Biology and Medicine

 
95 One Functional Switch Mediates Reversible and Irreversible Inactivation of a Herpesvirus Protease
 

Summary:  that require a viral protease with a concentration-dependent zymogen activation involving folding of two R... -26). Herpesvirus proteases require dimeriza- tion for activity (17, 18, 27-31) with binding affinities in the low... and engineering of a redox switch, which allowed protease ...

  

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

 

Collection: Biology and Medicine

 
96 An Autoinhibited Coiled-Coil Design Strategy for Split-Protein Protease Sensors
 

Summary:  death.1-4 Thus there is much interest in developing probes for measuring protease activities both... utilized for in Vitro measurement of protease activity and substrate specificities.5 However, many chemical... , which detect protease activity through changes in ...

  

Source: Ghosh, Indraneel - Department of Chemistry and Biochemistry, University of Arizona

 

Collection: Chemistry

 
97 Molecular Cell 23, 2535, July 7, 2006 2006 Elsevier Inc. DOI 10.1016/j.molcel.2006.05.019 A Branched Pathway Governing the Activation
 

Summary:  protease (His-A1,3,4 in Figure 3B). To test whether these mutants blocked cleavage by IVB in the context... serine protease, CtpB. Finally, we show that CtpB, like IVB, triggers sK activation by cleav- ing A. We... propose that the IVB serine protease ...

  

Source: Rudner, David - Department of Microbiology and Molecular Genetics, Harvard University

 

Collection: Biology and Medicine

 
98 Biochem. J. (2011) 435, 116 (Printed in Great Britain) doi:10.1042/BJ20100965 1 REVIEW ARTICLE
 

Summary:  they have, how active a given protease is within particular tissues in the human body, and how... factors. Considerable effort is required to resolve the detailed activities of proteases in vivo... activation, blood coagulation and many others. The roles of proteases ...

  

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

 

Collection: Biology and Medicine

 
99 Inhibition of extracellular protease secretion by Aspergillus niger using cell
 

Summary:  . Two replicate samples were taken for each time point. Protease activity measurement Extracellular... density of 1.00. One unit of protease activity was defined as a change of one absorbance unit per h at 280... ). Extracellular protease activities were expressed in ...

  

Source: Gu, Tingyue - Department of Chemical and Biomolecular Engineering, Ohio University

 

Collection: Biotechnology ; Chemistry

 
100 868 nature structural biology volume 6 number 9 september 1999 Retroviral proteases including HIV-1 protease, which range in
 

Summary:  is the folding/dimerization of the protease domain and formation of an active site that is capable of catalyzing... the processing activity, possibly by influencing protease dimerization10. Apart from a report that TFP and its... in mature-like enzymatic activity and the appearance of ...

  

Source: Clore, G. Marius - Laboratory of Chemical Physics. National Institutes of Health

 

Collection: Biology and Medicine


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