Summary:  rhinovirus 3C protease, SUMOstar, tobacco etch virus protease, and thrombin) by use of a panel of 94... tag removal. Common proteases include entero- kinase [1], factor Xa [2], human rhinovirus 3C protease... (HRV ...

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

Collection: Biotechnology ; Biology and Medicine

Summary:  with an inhibitor with F2Abu at P1, (e) HRV2 3C protease in complex with AG7088, (f) HRV2 2A protease, (g) FMDV... group.133 The structure of this inhibitor in complex with the 3C protease of HRV2 has also ...

Source: Tong, Liang - Department of Biological Sciences, Columbia University

Collection: Biology and Medicine

Summary:  and rhinoviral 3C proteases are widely used as reagents for endoproteolytic re- moval of affinity tags from... in the TVMV protease/peptide cocrystal structure. As shown in Figure 3(C), the only interac- tion made... structure of a catalytically inactive mutant TVMV ...

Source: Waugh, David S.- Macromolecular Crystallography Laboratory, National Cancer Institute

Collection: Biology and Medicine

Summary:  individuals [2]. Thus, there is no `wild-type' HIV-1 protease, but rather a complex mixture of related... is accompanied by a large shift in the flaps of both subunits (Figure 3c). In some enzymes (for example, RSV... .1 commentreviewsreportsdepositedresearchinteractionsinformationrefereedresearch Protein ...

Source: Wlodawer, Alexander - Macromolecular Crystallography Laboratory, National Cancer Institute

Collection: Chemistry ; Biology and Medicine

Summary:  important enzymes like the retroviral proteases [22], renin [23], hepatitis A3C protease [24], interleukin 1... pH in the schizont stage (Figs. 3C and F). The ring stage however presented a stark difference... activities in a complex mixture like ...

Source: Balaram , P. - Molecular Biophysics Unit, Indian Institute of Science, Bangalore

Collection: Biology and Medicine

Summary:  are mediated by the picornavirus 3C-type proteases, which are structurally similar to serine proteases like... -site nucleophile (1, 3). Because they play an essential role in viral replication, 3C proteases are viewed... ...

Source: Waugh, David S.- Macromolecular Crystallography Laboratory, National Cancer Institute; Wlodawer, Alexander - Macromolecular Crystallography Laboratory, National Cancer Institute

Collection: Biology and Medicine ; Chemistry

Summary:  | _____________________________Introduction to Protease Inhibitors__________________________________3 _____________________________c... The cpmplete Guide for Protease Inhibition cPmplete protection... cPmplete convenience www... .roche-applied-science.com #12;Proteases are ubiquitous in ...

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

Collection: Biotechnology ; Biology and Medicine

Summary:  in the presence and absence of the inhibitor (Fig. 3C). Strikingly, a 13°C increase in Tm was observed... Communication between the active sites and dimer interface of a herpesvirus protease revealed... of the active site and the dimer interface of wild-type protease in solution. Positional scanning ...

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

Collection: Biology and Medicine

Summary:  structure of protease- substrate complexes and applied it to a large and diverse set of cleavable... -substrate interactions have been characterized through crystallization of HIV-1 protease in complex with various... of the ten endogenous substrates in complex with a de-activated HIV-1 ...

Source: Gray, Jeffrey J. - Department of Biomolecular and Chemical Engineering, Johns Hopkins University

Collection: Chemistry ; Biology and Medicine

Summary:  Isatin Compounds as Noncovalent SARS Coronavirus 3C-like Protease Inhibitors Lu Zhou, Ying Liu... against SARS CoV 3C-like protease. Substitutions at the N-1 and C-5 positions were examined to elucidate... the differences in substrate binding sites of the rhinovirus ...

Source: Luhua, Lai - Institute of Physical Chemistry, Peking University

Collection: Chemistry ; Biotechnology

Summary:  in the SwissProt database. To bias the screen for previously unidentified proteases in the PC-3 cDNA, uPA c... biochemistry: Use of macromolecular protease inhibitors to dissect complex biological processes and identify... of protease­inhibitor ...

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

Collection: Biology and Medicine

Summary:  proteases and/or characterize the activity of a particular protease within complex mixtures. Plant genomes... Breakthrough Technologies Activity Profiling of Papain-Like Cysteine Proteases in Plants1 Renier A... . However, activity of many proteins, such as transcription factors, kinases, and ...

Source: Bogyo, Matthew - Department of Microbiology and Immunology, Stanford University

Collection: Biology and Medicine

Summary:  MA. 2004. Genetic screen for mon- itoring severe acute respiratory syndrome coronavirus 3C... HIV-1 Protease Catalytic Efficiency Effects Caused by Random Single Amino Acid Substitutions... immunodeficiency virus type 1 (HIV-1) protease was chosen as a model protein to quantify protein's tolerability

Source: Dean, Matthew D. - Department of Ecology and Evolutionary Biology, University of Arizona; Nachman, Michael - Department of Ecology and Evolutionary Biology, University of Arizona

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  , and it at least partially accounts for the activity of the inhibitor against target proteases (Figure 3C... Structure Article The Structure of Chagasin in Complex with a Cysteine Protease Clarifies... of the signature member of the I42 family, cha- gasin, in complex ...

Source: Sali, Andrej - Department of Biochemistry and Biophysics, University of California at San Francisco

Collection: Biology and Medicine ; Biotechnology

Summary:  -form of LP-149 complexed with FIV D30N protease, PDB accession code 3fiv.9 These FIV-PR structures provided... in Figures 3(c), 3(f), 4(c), and 4(f), with numerical values in Table I. In all cases, the improved versions... in the process. Optimal sizing of the P3/P3 side-chains is also essen- tial. As shown ...

Source: Sanner, Michel - Department of Molecular Biology, Scripps Research Institute

Collection: Chemistry ; Biology and Medicine

Summary:  localization, and in many cases act in the context of complex networks that comprise distinct proteases... - olytic enzymes, such as most membrane-type serine proteases60,61 , have a complex mosaic structure... of a complex protease catalogue will facilitate the ...

Source: Overall, Christopher M. - Department of Oral Biological and Medical Sciences, University of British Columbia

Collection: Biology and Medicine

Summary:  of protease & phosphatases & for proteases & assays Protease & Phosphatase Inhibitors & Proteases Protease... & Phosphatase Inhibitors & Proteases #12;ProteaseArrestTM Cat. # 786-108, 786-329 A Broad Range ...

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

Collection: Biotechnology ; Biology and Medicine

Summary:  .[13] The recent structures of the calcium-dependent protease calpain 2 in complex with the inhibitory... ] or TIMPs[19] bury when complexed with their respective sub- strates. The majority of competitive protease... - nines. The enzyme is still active; small-molecule substrates can be hydrolyzed by ...

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

Collection: Biology and Medicine

Summary:  . 3C). Because ProSGPB was capable of interacting with folded SGPB, we explored its ability... ) on the free energy profile (from Figs. 3C, 4A). Unlike LP, the SGPB native state (N) is marginally stable... , S.D., and Agard, D.A. 1998. Structure of -lytic protease ...

Source: Agard, David - Department of Biochemistry and Biophysics, University of California at San Francisco

Collection: Biotechnology ; Biology and Medicine

Summary:  specificities of the predicted forms of the viral protease, the 3C-like protease (NS6) and the 3CD-like protease... in viral replication, the viral chymotrypsin-like protease. The sapovirus protease ...

Source: Lübeck, Universität zu - Institute for Signal Processing

Collection: Computer Technologies and Information Sciences

Summary:  Lon-AP structure, the nucle- otide binding site of each subunit contains an ADP molecule (Fig. 3c). The protease... of these regions between related bacterial ATP- dependent proteases (Fig. 3c). In BsLon-AP, amino acids Gly354... of the complex, ...

Source: structure and motility & Molecular Machines.

Collection: Biology and Medicine

Summary:  mechanism of serine proteases: reexamination of the pH dependence of the histidyl lJl3C2-H coupling con... inactivation than in the mam- malian proteases or a more complex role for the precursor. Indeed, in the two... of an inhibitory complex of cc-lytic ...

Source: Agard, David - Department of Biochemistry and Biophysics, University of California at San Francisco

Collection: Biotechnology ; Biology and Medicine

Summary:  Enteropeptidase Thrombin 3C protease Factor Xa a b s t r a c t Although they are often exploited to facilitate... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 00 ...

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

Collection: Biotechnology ; Biology and Medicine

Summary:  on the availability of the structure of HIV1 protease, both as an apoenzyme and in complexes with inhibitors6... cat i o n s Crystal structure of XMRV protease differs from the structures of other retropepsins Mi Li... determined the crystal structure of the protease encoded by xenotropic murine leukemia ...

Source: Baker, David - Center for Nanotechnology and NanoTechnology & Department of Biochemistry, University of Washington at Seattle; Wlodawer, Alexander - Macromolecular Crystallography Laboratory, National Cancer Institute

Collection: Biology and Medicine ; Biotechnology ; Chemistry

Summary:  by protease XXIV. Figure 3C, insets, show families of original current traces from cells expressing meag... V incre- ments every 15 s. The I-V relation in Fig. 3C shows averaged meag current (Imeag) and IKCNH2... represent IKr and are similar (see also IKCNH2 I-V plot in Fig. ...

Source: Kamp, Tim - Molecular and Cellular Pharmacology Training Program & Department of Physiology, University of Wisconsin at Madison

Collection: Biology and Medicine

Summary:  -soluble extracts (Figures 3B and 3C). Thus vinckepain-2 is translated and is enzymically active in erythrocytic... in mediating activity and specificity of vinckepain-2, a papain-family cysteine protease of rodent malaria... proteases of Plasmodium falciparum, known as falcipains, have been identified as ...

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

Collection: Biology and Medicine

Summary:  -extracellular protease secretion (eps) F(B) Localization of the GFP extracellular protease secretion (eps) complex at one... Induced by Protease GradientsAbstract Motion in Mucus Induced by Protease Gradientsy Mucus is a hydrogel... bacteria break through this line of defense O r h ...

Source: Heller, Eric - Departments of Chemistry & Physics, Harvard University

Collection: Materials Science ; Physics

Summary:  are shielded from solvent in the bi-molecular complexes involving serine proteases and different types... a suffi- cient number of PDB [17] structures containing complexes of serine proteases with respective... ), named here as a secondary datamart, and three prototype complexes, ...

Source: Neshich, Goran - Brazilian Agricultural Research Corporation & Instituto de Biologia, Universidade Estadual de Campinas

Collection: Biology and Medicine

Summary:  ,13]. The crystal structure of catalytically inactive TEV protease in complex with a peptide substrate [12] revealed... . Similarly, comparison of the crystal structure of TEV protease in complex with a peptide substrate... with the experimentally determined crystal structure of TEV ...

Source: Wlodawer, Alexander - Macromolecular Crystallography Laboratory, National Cancer Institute

Collection: Chemistry ; Biology and Medicine

Summary:  terminus of pep52 and crosslinking with a homobifunctional reagent (Fig. 3C). An in vitro analysis revealed... chemotherapeutic agents7. HIV-1 protease is critical to viral maturation and infectivity8,9, and is a primary... target in AIDS chemotherapy10­12. To treat AIDS, attempts have been made to inactivate the ...

Source: Park, Sang-Hyun - School of Biological Sciences, Seoul National University; Raines, Ronald T. - Departments of Biochemistry & Chemistry, University of Wisconsin at Madison

Collection: Biology and Medicine ; Chemistry

Summary:  (Fig. 3C), but in contrast expression of TEV protease prevented DNA replication (Fig. 3B), implying... is affected by TEV protease cleavage of Cdc23 (Mcm10) in fission yeast Xiaowen Yang1,2, Juraj Gregan1... by the TEV protease. Results: Insertion of a TEV protease ...

Source: Kearsey, Stephen - Department of Zoology, University of Oxford

Collection: Biology and Medicine

Summary:  in different species.Finally, and in keeping with the observed protease complexity, recent studies... specific proteases from complex biological samples. Despite the promise of such techniques, as shown... from complex mixtures. Retained proteases could be detected ...

Source: Overall, Christopher M. - Department of Oral Biological and Medical Sciences, University of British Columbia

Collection: Biology and Medicine

Summary:  , and the signal from the silver staining became very weak (Figure 3C). This observa- tion reflects that the Au... Energy Transfer-Based Multiplexed Assay of Proteases by Using Gold Nanoparticle and Quantum Dot... and sensitive assay of proteases and their inhibition in a high-throughput manner is of great ...

Source: Kim, Hak-Sung - Department of Biological Sciences, Korea Advanced Institute of Science and Technology

Collection: Biology and Medicine

Summary:  with stringent sequence speciWcity have become important tools [10]. The 3C-like proteases encoded by certain... of this type are rhinovirus 3C protease (also known as PreScission pro- tease) and the nuclear inclusion... -speciWc processing of fusion proteins by ...

Source: Wlodawer, Alexander - Macromolecular Crystallography Laboratory, National Cancer Institute

Collection: Chemistry ; Biology and Medicine

Summary:  @bcm.tmc.edu). ................................................................. Structure of a serpin±protease complex shows inhibition by deformation James A. Huntington, Randy J. Read... ± protease complex and show the mechanism of inhibition. The conformational change is initiated by reaction... ...

Source: Read, Randy J. - Department of Haematology, University of Cambridge

Collection: Biology and Medicine

Summary:  fourresiduesproximaltothescissilepeptidebond,andtheobservation that large PREs are observed for both sides of the active site (see Fig. 3c... of the encounter complexes adopt the same subunit orientation as in the mature protease, accounting for the very... ,j are the distance matrices in a calculated ...

Source: Clore, G. Marius - Laboratory of Chemical Physics. National Institutes of Health

Collection: Biology and Medicine

Summary:  models. Modifications of these models were made to include these. Figures 3a­3c are the normal... . However, heterologous protein production is often severely hampered by fungal proteases. In this work... , and dissolved oxygen tension (DO)--on extracellular protease inhibition and heterologous protein ...

Source: Gu, Tingyue - Department of Chemical and Biomolecular Engineering, Ohio University

Collection: Biotechnology ; Chemistry

Summary:  and Falcipain-3 Shoba Subramanian1¤a , Markus Hardt2¤b , Youngchool Choe3¤c , Richard K. Niles2 , Eric B... Hemoglobin Cleavage Site-Specificity of the Plasmodium falciparum Cysteine Proteases Falcipain-2... , California, United States of America Abstract The Plasmodium falciparum cysteine proteases ...

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

Collection: Biology and Medicine

Summary:  with BAPTA-AM (Fig. 3C, lane 4). In contrast to TgMIC2, the DG protein, GRA1, is present in extracellular... within the active site of the protease. A, recombinant TgPI-1 (rTgPI-1) forms a complex with purified... Functional Analysis of Toxoplasma gondii Protease Inhibitor 1* Received for ...

Source: Morris, James C. - Department of Genetics, Biochemistry and Life Science Studies, Clemson University

Collection: Biology and Medicine

Summary:  without surface glycoprotein (referred to herein as empp) (Figure 3C) on ice, hence allowing virus... Cleavage of the SARS Coronavirus Spike Glycoprotein by Airway Proteases Enhances Virus Entry... glycoproteins through cleavage by either intracellular or extracellular proteases. In order to gain insight

Source: Ecole Polytechnique, Centre de mathématiques

Collection: Mathematics

Summary:  structures of MA190, GA216 + MA190 and GL216 + MA190 a-lytic proteases, both free and in complex... complexes with position 216 mutants of MA190 a-lytic protease. (a) Re-refined structures of complexes... with MA190 a-lytic protease. (b) ...

Source: Agard, David - Department of Biochemistry and Biophysics, University of California at San Francisco

Collection: Biotechnology ; Biology and Medicine

Summary:  Protease · Branden & Tooze, Chapter 11 · An enzyme that hydrolyzes the peptide bond ­ works without... although thermodynamically unstable ­ study the mechanism of serine protease ­ determinants of specificity... · Role of protease in disease development ­ implicated in numerous hereditary diseases ­ normal

Source: Park, Sheldon - Department of Chemical and Biological Engineering, State University of New York at Buffalo

Collection: Biology and Medicine ; Biotechnology

Summary:  structure of Kaposi's sarcoma-associated herpesvirus protease in complex with a hexapeptide transition state... . Ordering of unstructured regions of KSHV protease The crystal structure of the KSHV protease complexed... that substrate buries upon binding is calculated from the structure ...

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

Collection: Biology and Medicine

Summary:  +FP ×100; (3c) Fsc = 2×Pre×Rec Pre+Rec (3d) MCC = TP×TN +FP×FN (TP+FP)(TP+FN)(TN +FP)(TN +FN) (3c) where... /btn538 Sequence analysis Protease substrate site predictors derived from machine learning on multilevel... publication October 29, 2008 Associate Editor: Burkhard Rost ABSTRACT ...

Source: Chu, Hao-hua - Department of Computer Science and Information Engineering, National Taiwan University; Hsu, Wen-Lian - Institute of Information Science, Academia Sinica (Taiwan)

Collection: Computer Technologies and Information Sciences

Summary:  -Glu-Gly-Arg-pNA effec- tively (Figure 3B). This activation step occurred in a time- dependent manner (Figure 3C... ), and the exocytosed serpin binds to the uPA/uPAR complex to dampen the protease-mediated activation response. Some... substrate of the human epithelium serine protease ...

Source: Sali, Andrej - Department of Biochemistry and Biophysics, University of California at San Francisco

Collection: Biology and Medicine ; Biotechnology

Summary:  Genes encoding proteases within any genome are easily identified, and three-dimensional structures... of many proteases have been solved. Mostproteolyticcleavagemechanismsandpro... - tors. Transgenic approaches have been used for targeted deletions of protease- or protease inhibitor

Source: Bogyo, Matthew - Department of Microbiology and Immunology, Stanford University

Collection: Biology and Medicine

Summary:  ). The LMA2-based reaction is sensitive to each of the characterized fusion inhibitors (Fig. 3C) that also... inhibit the cytosol-driven vacuole fusion reaction (refs. 14, 16, and 18 and Fig. 3C). Since I2 B (LMA2... and protease B inhibitor 2 (I2 B ). Here we show that the second low ...

Source: Haas, Albert - Institute for Cell Biology, Universität Bonn

Collection: Biology and Medicine

Summary:  ..........................................................................................................2 Description 2 Components 2 Storage 2 HRV 3C Protease... -column cleavage 6 Removal of HRV 3C Protease 6 Troubleshooting Guide... . User Protocol TB420 Rev. A 0804 ...

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

Collection: Biotechnology ; Biology and Medicine

Summary:  structure of the native subtype F HIV-1 protease (PR) in complex with the protease inhibitor TL-3. We have... ±3 24±8 180±29 PDB ID code 2P3B 2P3A 2P3C 2P3D All structures were solved and refined in the space group... assays and crystallization trials. Crystallization The ...

Source: Wlodawer, Alexander - Macromolecular Crystallography Laboratory, National Cancer Institute

Collection: Chemistry ; Biology and Medicine

Summary:  the eight monomers are arranged in a barrel-shaped architecture. In the protease complex, monomers associate... members, indicating similar catalytic pathways despite different subtrate specificities (Figure 3C... al. (2002) Tetrahedral aminopeptidase: a novel large protease ...

Source: Ecole Polytechnique, Centre de mathématiques

Collection: Mathematics

Summary:  construct (Figure 3c). Similar FRET-based fluorescent reporters have been generated for both serine [39... to measure and are controlled by a complex set of mechanisms that are independent of transcription... - teins, nucleic acids and small-molecule binding partners. This daunting level of complexity

Source: Bogyo, Matthew - Department of Microbiology and Immunology, Stanford University

Collection: Biology and Medicine

Summary:  phase are independent of Pro concentration [Fig. 3(c)], and they indicate that the formation... .3) 10 2 3.2 ( 0.3) 10 2(a) KM ( M) 11 2 24 6a ki (s 1 ) 1.8 ( 0.1) 10 2 5.7 ( 0.5) 10 3(c) kcat (s 1 ) 4... of -lytic protease complexed with its pro region. Nat Struct ...

Source: Agard, David - Department of Biochemistry and Biophysics, University of California at San Francisco

Collection: Biotechnology ; Biology and Medicine

Summary:  of the difficulty in understanding protease biology is a direct result of the complexity of regulation, localization... . 163, 1398­1408 13 Noessner, E. et al. (2002) Tumor-derived heat shock protein 70 peptide complexes... , an extracellular receptor for binding and uptake of Hsp70-peptide complexes. J. Cell ...

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

Collection: Biology and Medicine

Summary:  of protease (C). The complex decomposes into a modified receptor (RV ), which is subsequently recycled back... that is in the intermediate #12;126 complex (CAF). Angiostatin (A) combines with active protease to form inactive pro- tease... vp 1 + v + K2 (V (x, 0, t) - v (x, t)) , (5.44) c t = K1 vp 1 + v - ...

Source: Plank, Mike - Department of Mathematics and Statistics, University of Canterbury

Collection: Mathematics

Summary:  on chromosome 16p13.3 within a 2.5-Mb complex of serine protease genes. Although at least 7 of the 14 genes... epithelial cell line (Fig. 3C). The tran- script also was detected in varied human tumor xenografts... transcript was detected in a number of trans- formed human epithelial cell lines (Fig. ...

Source: Sali, Andrej - Department of Biochemistry and Biophysics, University of California at San Francisco

Collection: Biology and Medicine ; Biotechnology

Summary:  the protease active site. Instead, the catalytic site is fully functional in the complex. Pro region inhibition... is fully formed and functional within the pro region-protease complex. Further, this study indicates... spectrum of the complex is nearly identical to the sum of the ...

Source: Agard, David - Department of Biochemistry and Biophysics, University of California at San Francisco; Rader, Stephen - Chemistry Program, University of Northern British Columbia

Collection: Biology and Medicine ; Biotechnology ; Chemistry

Summary:  substrate/inhibitor complexes. Further, mutation of two protease residues near the active site have... of the refolding reaction (Figures 1 and 3c and Table 1). Interestingly, binding to the native state improves... shown to bind in the protease active site in the native state ...

Source: Agard, David - Department of Biochemistry and Biophysics, University of California at San Francisco

Collection: Biotechnology ; Biology and Medicine

Summary:  polyprotein. Two viral proteases, 2A and 3C, co-translationally cleave the viral polyprotein into mature pep... 3C (Pro3C); and the viral RNA polymerase (3DPOL). © 1999 Nature America Inc. · http... - tides21 . In addition to the cleavage ...

Source: Campbell, Kevin P. - Department of Physiology and Biophysics, University of Iowa

Collection: Biology and Medicine

Summary:  HIV protease inhibitors and nuclear lamin processing: Getting the right bells and whistles Steven... design has been the development of drugs that in- hibit the protease cleaving the polypro- teins... target the HIV protease and not the hundreds of other proteases re- quired for human health

Source: Clarke, Steven - Department of Chemistry and Biochemistry, University of California at Los Angeles

Collection: Biology and Medicine

Summary:  colonies than WT E. coli after 6 h incubation with NE (Figure 3C). The observation that the presence... Biochem. J. (2004) 379, 107­118 (Printed in Great Britain) 107 The periplasmic serine protease... proteases of widely varying substrate specificity. To help elucidate the physiological function of ecotin

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

Collection: Biology and Medicine

Summary:  complexes of a, -lytic protease and b, subtilisin. For -lytic protease only the C-terminal domain of the pro... ­mature protease complex The determination of the structure of the pro region­mature -lytic protease com- plex... . The structure of the ...

Source: Baker, David - Center for Nanotechnology and NanoTechnology & Department of Biochemistry, University of Washington at Seattle

Collection: Chemistry ; Biotechnology

Summary:  ; PLP, papain-like protease; 3Clpro, 3C like protease; IBV, avian infectious bronchitis virus; TGEV... His containing substrate Ac-T- S-A-V-L-H-(ACC) against the related 3C-like proteases from IBV and MHV... -A-V-L-H-(ACC) shows that IBV and MHV ...

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

Collection: Biology and Medicine

Summary:  in the cytoplasmic or nuclear fractions of Tpap / and Tpap­/­ testes (Fig. 3C). The specific reduction of TAF10... is severely impaired in Tpap­/­ testes by insufficient transport of TAF10 into the nucleus (Fig. 3, C and D... in the nucleus of round spermatids in Tpap­/­ mice (Fig. ...

Source: Arnold, Jonathan - Nanoscale Science and Engineering Center & Department of Genetics, University of Georgia; Bogyo, Matthew - Department of Microbiology and Immunology, Stanford University

Collection: Biology and Medicine ; Biotechnology

Summary:  was also detected with greater sensitivity by microarrays (Figures 3C, S6). The enhanced sensitivity... Microarray Platform for Profiling Enzyme Activities in Complex Proteomes Stephan A. Sieber, Tony S... to develop methods for the comparative analysis of large numbers of proteins in complex biological ...

Source: Cravatt, Benjamin - Department of Cell Biology, Scripps Research Institute

Collection: Biology and Medicine

Summary:  the substrate peptide being cleaved. As shown in Fig- ure 3c, before PSA addition, the NPR nanosensor showed... seminal plasma by column chromatography, eliminating all known PSA complexes and retaining its protease... Time-Resolved Single-Step Protease Activity Quantification Using ...

Source: Zhang, Xiang - Department of Mechanical Engineering, University of California at Berkeley

Collection: Physics ; Engineering

Summary:  the substrate peptide being cleaved. As shown in Fig- ure 3c, before PSA addition, the NPR nanosensor showed... known PSA complexes and retaining its protease fraction. Cleavage of the substrate peptide immobilized... Time-Resolved Single-Step Protease Activity Quantification Using ...

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

Collection: Biology and Medicine

Summary:  , Medivation, Inc. higaki_97@yahoo.com McGrath, M. Post 1986-1993 Structural biology of proteases... , CA DeCamp, D. Post 1988-1993 Elucidation of the active site of HIV proteases University of Delaware... , L. Post 1988-1994 Structural biology of the HIV protease University of Miami, PhD, 1988 Genencot

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

Collection: Biology and Medicine

Summary:  of Specificity: a-Lytic Protease Complexes with Analogues of Reaction Intermediates+?$ Roger Bone,! Dan Frank... substrates. Yet, on the basis of the structure of a complex formed between a-lytic protease and R3-boro... the crystal structures of the complexes formed between a-lytic ...

Source: Agard, David - Department of Biochemistry and Biophysics, University of California at San Francisco

Collection: Biotechnology ; Biology and Medicine

Summary:  , but also distinct differences in mechanism and structure. Thus, the picornaviral 3C cysteine protease... B, and the 3C protease (Ryan and Flint, 1997). This enzyme carries out all remaining cleavages... ). References Allaire,M., Chernaia,M.M., Malcolm,B.A. and ...

Source: Guarne, Alba - Department of Biochemistry and Biomedical Sciences, McMaster University

Collection: Biology and Medicine

Summary:  by the main protease (Mpro , also often called 3C- like protease) and the papain-like cysteine protease (PL2... RESEARCH ARTICLE Liberation of SARS-CoV main protease from the viral polyprotein: N... @biochem.uni-luebeck.de Received October 27, 2009; accepted November ...

Source: 

Collection: Computer Technologies and Information Sciences

Summary:  protea- some complex. Distinct Proteases Catalyze Signal Sequence Cleavage and Signal Peptide Processing... value determined by our assay was 50 nM for (Z-LL)2 ketone (Fig. 3C) and 1­2 M for (Bio-LL)2 ketone (not... by a Protease Activity That Is Specifically Blocked by a Novel Cysteine ...

Source: Bogyo, Matthew - Department of Microbiology and Immunology, Stanford University

Collection: Biology and Medicine

Summary:  structures of protease­inhibitor complexes. Using this model, A(m; i) increases with the difference... in the genetic algorithm, not natural mutation of the protease. 16 #12; repair attempt begins again. Such complex... using structures of 12 protease­inhibitor ...

Source: Belew, Richard K. - Departments of Computer Science and Engineering & Cognitive Science, University of California at San Diego

Collection: Computer Technologies and Information Sciences

Summary:  share conformational features present in all crystal structures of serpins and serpin-protease complexes... . The protease is no longer trapped in a covalent complex as opposed to wild type LEI. As a result, equilibrium... ;0 !( ),!3 3 )! *! 2 , ) & 8 0 $ ' 3 # # 9 3* = << '* ( ! !; , >? ( ) * ( 1 ...

Source: Ecole Polytechnique, Centre de mathématiques

Collection: Mathematics

Summary:  are becoming shielded from a solvent upon complex formation among serine proteases and different types... ] structures containing complexes of serine proteases with respective inhibitors. It became clear very rapidly... of the interfaces around active sites of serine proteases (even if ...

Source: Neshich, Goran - Brazilian Agricultural Research Corporation & Instituto de Biologia, Universidade Estadual de Campinas

Collection: Biology and Medicine

Summary:  medium of Adye & Mateles (1964); -N = AM without NaNO3; -C/N = AM without sucrose or NaNO3; ND... reservesrepresentsubstantialcarbonandnitrogenresources available to the fungus during seed infection. Fungal proteases are an important hydrolytic tool... , allowing the utilization of seed reserves. Soybean seed protein induces ...

Source: Cotty, Peter J. - School of Plant Sciences, University of Arizona

Collection: Biology and Medicine

Summary:  - cess. The surface projected along the QA and QB coordinates (Figure 3C) demonstrates the lack... contactsli2jl.3 1 5 r0ij rij 12 26 r0ij rij 10 " #( ) þ X non-native contacts;li2jl.3 C rij 12 ð1Þ... The Folding and Dimerization of HIV-1 Protease: Evidence for a Stable Monomer from ...

Source: Caflisch, Amedeo - Department of Biochemistry, Universität Zürich; Onuchic, José - Department of Physics, University of California at San Diego

Collection: Biology and Medicine ; Physics

Summary:  Classification of ATP-dependent proteases Lon and comparison of the active sites... , National Cancer Institute at Frederick, MD, USA ATP-dependent Lon proteases belong to the superfamily... of more than 100 Lon proteases from various sources. The presence of the catalytic dyad was experimentally

Source: Wlodawer, Alexander - Macromolecular Crystallography Laboratory, National Cancer Institute

Collection: Chemistry ; Biology and Medicine

Summary:  to the cortical granule is further supported by immunoblot analysis of cell surface complex with the anti-protease... chromatography. Lane 1, cell surface complex, 20 g/lane. Lane 2, affinity-purified protease extract, 10 g/lane. 8... The Cortical Granule Serine Protease CGSP1 of the Sea ...

Source: Wessel, Gary M. - Department of Molecular Biology, Cell Biology and Biochemistry, Brown University

Collection: Biology and Medicine

Summary:  a method for directly detecting protease activity in complex lysate mixtures. Future research will focus... An Autoinhibited Coiled-Coil Design Strategy for Split-Protein Protease Sensors Sujan S. Shekhawat... Versity of Arizona, Tucson, Arizona 85721 Received June 26, 2009; E-mail: ghosh@email.arizona.edu Abstract: ...

Source: Ghosh, Indraneel - Department of Chemistry and Biochemistry, University of Arizona

Collection: Chemistry

Summary:  lysosomal proteases. This inhibitory activity is specific of C3b and is supported by the C3c fragment... by lysosomal proteases is impaired in the presence of C3, C3b and C3c fragments (40-50% inhibition... cell line (U937) ...

Source: Ecole Polytechnique, Centre de mathématiques

Collection: Mathematics

Summary:  of herpes simplex virus protease/inhibitor complex, Biochemistry 36, 14023-14029. 24. Qiu, X., Janson, C. A... One Functional Switch Mediates Reversible and Irreversible Inactivation of a Herpesvirus Protease... ABSTRACT: Distinct mechanisms have evolved to regulate the function of proteolytic enzymes. Viral ...

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

Collection: Biology and Medicine

Summary:  to that of the various generations of 4, with the third generation again showing no activity (Figure 3c). We then tested... ­000 COMMUNICATION pubs.acs.org/JACS HIV Protease-Mediated Activation of Sterically Capped Proteasome Inhibitors... a cytotoxic, small- molecule proteasome inhibitor upon cleavage by HIV-1 ...

Source: Crews, Craig M. - Department of Molecular Biophysics and Biochemistry, Yale University

Collection: Chemistry ; Biology and Medicine

Summary:  protease activity at concentrations of up to seven times this concentration (Fig. 3c, lane 5). A low level... of residual activity was only detectable at much higher concentrations (Fig. 3c, lane 6). The mutant protein... critical for homotrimerization9 . As formation of a homotrimeric ...

Source: Meisler, Miriam - Department of Human Genetics, University of Michigan

Collection: Biology and Medicine

Summary:  of the flexible N-terminal helix on protease binding. As expected for a tight-binding inhibitory complex, the Pro... stability as a mechanism for protease longevity ERIN L. CUNNINGHAM, SHEILA S. JASWAL, JULIE L. SOHL... of the extracellular serine pro- tease, -lytic protease ( LP; EC 3.4.21.12) reveals a ...

Source: Agard, David - Department of Biochemistry and Biophysics, University of California at San Francisco

Collection: Biotechnology ; Biology and Medicine

Summary:  . C3c is fairly resistant to proteolysis by lysosomal proteases (12.5 degradation upon a 18h... complexes are delivered to lysosomes, where C3c fragments are generated upon lysosomal proteolytic... in the presence of C3, ii) this effect is C3-specific and involves the ...

Source: Ecole Polytechnique, Centre de mathématiques

Collection: Mathematics

Summary:  their transcription (48). While the methylation state of the chromosome 14 complex of serine protease genes has... proteases whose genes reside at the chromosome 14 complex, the propeptides of the MC chymases consist... .0 environment, most MC proteases remain in the macromolecular ...

Source: Sali, Andrej - Department of Biochemistry and Biophysics, University of California at San Francisco

Collection: Biology and Medicine ; Biotechnology

Summary:  B) peptide separated on 16.5%T, 3%C Tris-Tricine SDS-PAGE probed with A, anti-Smd1 sera (MJL3), 1... ), 1 : 50 dilution (lane 1); 1 : 100 dilution (lane 2); 1 : 200 dilution (lane 3). C, control sera (MJL... contents. We now found that the > 26 kDa band was only present in the reservoir tissue (Fig. ...

Source: Hamilton, Jo - Institute of Biological Sciences, University of Wales, Aberystwyth

Collection: Biology and Medicine

Summary:  viral proteases have been difficult, given the complexity of the the viral biology and intricacies... similar to spectra of DMP323 complexes with other mature protease variants studied by NMR26,27. This high... and signal assignments of human- immunodeficiency-virus-1 protease ...

Source: Clore, G. Marius - Laboratory of Chemical Physics. National Institutes of Health

Collection: Biology and Medicine

Summary:  University of California, San Francisco, USA In vivo, many proteases are synthesized as larger precursors... . During the maturation process, the catalytically active protease domain is released from the larger... -terminal pro regions have been shown to play a fundamental role in the folding of the associated protease

Source: Agard, David - Department of Biochemistry and Biophysics, University of California at San Francisco

Collection: Biotechnology ; Biology and Medicine

Summary:  determine its activity by localizing GUS expression in the anther under a light microscope (Figure 3c­ e... Isolation and characterization of a rice cysteine protease gene, OsCP1, using T-DNA gene... , cell death, cysteine protease, rice, tapetum, T-DNA insertional mutagenesis Abstract The T-DNA gene

Source: An, Gynheung - Department of Life Science, Pohang University of Science and Technology

Collection: Biotechnology ; Biology and Medicine

Summary:  classes of protease for exploring proteolytic function in complex and dynamic biological contexts. In all... of such biochemical complexity, this emphasizes the need to identify protease-cleaved substrates and not just enzyme... the potential to identify protease substrates in ...

Source: Overall, Christopher M. - Department of Oral Biological and Medical Sciences, University of British Columbia

Collection: Biology and Medicine

Summary:  , but involves forming a binary complex with plasminogen inducing a conformational change into an active protease... Proteases as therapeutics Charles S. CRAIK*1 , Michael J. PAGE* and Edwin L. MADISON *Department... Biosciences, 260 Littlefield Avenue, South San Francisco, CA 94080, U.S.A. Proteases are an ...

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

Collection: Biology and Medicine

Summary:  ) of the proteins in the membrane protease complex. In support of this idea, during sporulation the degradation... of A triggers intramembrane proteolysis by a conformational change in the membrane protease complex. We present... detectable cleavage by IVB (Figure 3C ...

Source: Rudner, David - Department of Microbiology and Molecular Genetics, Harvard University

Collection: Biology and Medicine

Summary:  ). As shown in Figure 3C, WB800 cells producing LipA did not transcribe htrB-lacZ at significantly elevated... levels in the cells producing LipA were slightly increased (Fig. 3C). To verify whether the production... factors, such as folding catalysts and "cleaning proteases". The ...

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  600 nm (Fig. 3A). As shown in Figure 3C, WB800 cells producing LipA did not transcribe htr... B-lacZ expression levels in the cells producing LipA were slightly increased (Fig. 3C). To verify whether... action of quality control factors, such as folding catalysts and "cleaning proteases". ...

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  and in complexes with inhibitors6. Although all retroviral proteases studied to date are structurally similar7... and HIV-1 proteases complexed with an efficient inhibitor of FIV protease. Proteins: Struct. Funct. Genet... publication b r i e F com mu n i cat i on s Crystal structure of ...

Source: Wlodawer, Alexander - Macromolecular Crystallography Laboratory, National Cancer Institute

Collection: Chemistry ; Biology and Medicine

Summary:  incubated with recombinant HAI-1 (Fig. 3C and D). Cells that were treated with HAI-1 be- fore the addition... of macromolecular protease inhibitors to dissect complex biological processes and identify a membrane-type serine... . Farady CJ, Egea PF, Schneider EL, Darragh MR, Craik CS. Structure of an ...

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

Collection: Biology and Medicine

Summary:  ). In addition, Rao's group also elucidated the complex structure of SARS 3C-like proteinase with a covalent... protease family both in cis- or in trans-cleavage assays. SUBSTRATE SPECIFICITY OF SARS 3C-LIKE PROTEINASE... as a transition state analog of the HIV ...

Source: Luhua, Lai - Institute of Physical Chemistry, Peking University

Collection: Chemistry ; Biotechnology

Summary:  protease domains) [3], (c) Atomic structures are available for all the domains in the macromolecule... for C3, C4, C5, C4b binding protein (C4BP), factor H (FH), properdin (P), and C9 [3]. In such cases... . To facilitate studies of the multidomain serine proteases of ...

Source: Ecole Polytechnique, Centre de mathématiques

Collection: Mathematics

Summary:  polypeptide Human rhinovirus 3C protease Methodological synchronization Isotopic labeling of peptides Peptide... . The native sequence of aPP allows inclusion of an N-terminal cleavage site for human rhinovirus 3C protease... rhinovirus ...

Source: Hardy, Jeanne - Department of Chemistry, University of Massachusetts at Amherst

Collection: Chemistry