Summary:  ribosome from the same source, T70S [6]. The earlier studies are reviewed extensively (e.g. [7­9]). More... to functional regions were proposed to support specific activities. Thus, proteins S5, S6 and S12 assist m... CAN STRUCTURES LEAD TO BETTER DRUGS? LESSONS FROM ...

Source: Martin, Jan M.L. - Department of Organic Chemistry, Weizmann Institute of Science

Collection: Chemistry

Summary:  in proximity to functional regions were proposed to support specific activities. Thus, proteins S5, S6 and S12... Correlating ribosome function with high-resolution structures Anat Bashan and Ada Yonath Department... ;Correlating ribosome function with high-resolution structures Anat Bashan and Ada Yonath ...

Source: Martin, Jan M.L. - Department of Organic Chemistry, Weizmann Institute of Science

Collection: Chemistry

Summary:  J.D. Puglisi (ed.), Biophysics and the Challenges of Emerging Threats, 121 RIBOSOME: AN ANCIENT... Threats, 121 © Springer Science + Business Media B.V. 2009 RIBOSOME: AN ANCIENT CELLULAR NANO... The ribosome is a ribozyme whose active site, the peptidyl trans- ferase center (PTC), is situated within

Source: Martin, Jan M.L. - Department of Organic Chemistry, Weizmann Institute of Science

Collection: Chemistry

Summary:  , respectively. At least two clus- ters of relative orientations were also found for the i-1 neighbors (Figure S6... led to insights into the struc- ture of the bacterial ribosome, but little is known about the 3D... organization of ribosomes in the context of translating polysomes. We employed cryoelectron ...

Source: Bedwell, David M. - Department of Microbiology, University of Alabama at Birmingham

Collection: Biology and Medicine

Summary:  . S6) (17, 18). A particular advantage of our strategy for quantitating mRNA levels... of runoff elongation (fig. S19). Overall, we found 143 non-AUG uORFs with evidence of translation (table S6... Resolution Using Ribosome Profiling Genome-Wide Analysis in Vivo of Translation with ...

Source: Economou, Tassos - Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology, Hellas

Collection: Biology and Medicine

Summary:  . S6) (17, 18). A particular advantage of our strategy for quantitating mRNA levels... of runoff elongation (fig. S19). Overall, we found 143 non-AUG uORFs with evidence of translation (table S6... Resolution Using Ribosome Profiling Genome-Wide Analysis in Vivo of Translation with ...

Source: Bedwell, David M. - Department of Microbiology, University of Alabama at Birmingham

Collection: Biology and Medicine

Summary:  Initiation of protein synthesis in eukaryotes requires the ordered assembly of ribosomal pre... moiety at the 5 end of mRNA, leading to 40S ribosome binding and scanning to the initiation codon... . This is followed by association with the 60S ribosomal subunit to form an active 80S ribosome (BOX 1

Source: Doudna, Jennifer A. - Department of Molecular and Cell Biology, University of California at Berkeley

Collection: Biology and Medicine

Summary:  RNA55 (http://www.nature.com/nature/journal/v438/n7067/extref/ nature04152-s6.mov, http... REVIEWS What recent ribosome structures have revealed about the mechanism of translation T. Martin... Schmeing1 & V. Ramakrishnan1 The high-resolution structures of ribosomal subunits published in 2000 have

Source: Joseph, Simpson - Department of Chemistry and Biochemistry, University of California at San Diego

Collection: Biology and Medicine ; Chemistry

Summary:  for the remaining four proteins (Figure S6). Protein levels confirm that paralog-specific phenotypes are not due... Functional Specificity among Ribosomal Proteins Regulates Gene Expression Suzanne Komili,1... RNAs. Intriguingly, these paralog-specific effects are limited to a distinct subset of duplicated ...

Source: Roth, Frederick - Department of Biological Chemistry and Molecular Pharmacology, Harvard University

Collection: Biotechnology ; Biology and Medicine

Summary:  associated with the path of the mRNA through the ribosome (Figure 1). For example, r-proteins S2, S6,S18,and... (pale blue), S6 (cyan), S7 (blue), S11 (teal), and S18 (yellow) are colored for reference. (B) Interface... .01.006 In this issue of Molecular Cell, Kaberdina et al. (2009) show ...

Source: Bedwell, David M. - Department of Microbiology, University of Alabama at Birmingham

Collection: Biology and Medicine

Summary:  not modeled explicitly. A rotation series of the small (Figure S5) and large subunits (Figure S6) shows... contains a helices and b strands with an S6p-like config- uration (see Intersubunit Bridges section... of S6p in eukaryotes, but this domain of S-IV may be ...

Source: Sali, Andrej - Department of Biochemistry and Biophysics, University of California at San Francisco

Collection: Biology and Medicine ; Biotechnology

Summary:  that are disordered in H50S are well resolved in D50S (6, 28). Among the well-ordered features are the intersubunit... -Resolution Structures of Ribosomal Particles from Mesophilic, Thermophilic, and Halophilic Bacteria at Various... , Israel and Max-Planck-Research Unit for Ribosomal Structure, Hamburg 22603, ...

Source: Martin, Jan M.L. - Department of Organic Chemistry, Weizmann Institute of Science

Collection: Chemistry

Summary:  - mentary Figure S6). In particular, sw1 adopts similar helical conformations in EF-TuKGDPNP and EF-G-2... (Figure 5; Supplementary Figure S6) from three to five independent reactions. c Biphasic kinetics (rate... spring- loaded interactions of its conserved Thr62 with the g-phos- phate of GTP (Supplementary ...

Source: MacMillan, Andrew - Department of Biochemistry, University of Alberta

Collection: Chemistry ; Biology and Medicine

Summary:  RpS6 leads to a reduction in RpS6 protein levels (Fig. 2B). Moreover, RNAi against other ribosomal... RNA treatment against ribosomal proteins RpS6 or RpL19 results in a significant effect on translation from DCV... ...

Source: Higgins, Darren - Department of Microbiology and Molecular Genetics, Harvard University

Collection: Biology and Medicine

Summary:  , L.M., Craven, G.R., and Noller, H.F. 1988. Interaction of ribosomal proteins, S6, S8, S15 and S18... 627, C739, C754, C770, and G838. Small subunit proteins S6, S8, S11, S15, S18, and S21 bind... . Shown are protection by primary binding proteins (S4, S8, S17, and S20), secondary binding ...

Source: Joseph, Simpson - Department of Chemistry and Biochemistry, University of California at San Diego

Collection: Biology and Medicine ; Chemistry

Summary:  of a disulfide bridge between ribosomal proteins S6 and L2 designed to restrict intersubunit movement, resulting... residues introduced by directed mutagenesis into ribosomal proteins S6 (D41C), S11 (E75C), and L9 (N11C... then associated to create two kinds of ...

Source: Ha, Taekjip - Department of Physics, University of Illinois at Urbana-Champaign

Collection: Physics ; Chemistry ; Biology and Medicine

Summary:  , University of California at Berkeley, Berkeley, CA, USA Abstract. The ribosome is a dynamic machine... initiation factors to promote efficient mRNA recruitment and ribosomal recognition of the start codon than... are needed for eubacterial initiation. The mechanisms by which these initiation factors promote ribosome

Source: Doudna, Jennifer A. - Department of Molecular and Cell Biology, University of California at Berkeley

Collection: Biology and Medicine

Summary:  for ribosomal proteins S6, S8, S11, S15 and S18 (refs 23, 32, 33). In articles NATURE |VOL 400 |26 AUGUST 1999... . Science 277, 1262­1267 (1997). 32. Gregory, R. J. et al. Interaction of ribosomal proteins S6, S8, S15... crystal structure into the electron density. ...

Source: Ramakrishnan, Venki - Laboratory of Molecular Biology, MRC

Collection: Biology and Medicine

Summary:  , there is noit is located more toward the side of the shoulder, a protein density corresponding to proteins S6p... Ribosome from Saccharomyces cerevisiae-- tRNA-Ribosome and Subunit-Subunit Interactions (Green and Noller... Center mark structures indicate that ribosomes are ...

Source: Economou, Tassos - Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology, Hellas; Sali, Andrej - Department of Biochemistry and Biophysics, University of California at San Francisco

Collection: Biology and Medicine ; Biotechnology

Summary:  (including S2, S5, S6, S7, S9, S11 and S18) but also with several domains of the 16S RNA. These interactions... of stalled ribosomes when translation occurs on truncated mRNAs in bacteria, followed by the degradation... acting as both a tRNA and an mRNA. tmRNA first enters the decoding site of stuck ...

Source: Rennes, Université de - Faculté de Pharmacie

Collection: Biology and Medicine

Summary:  of a disulfide bridge between ribosomal proteins S6 and L2 designed to restrict intersubunit movement, resulting... residues introduced by directed mutagenesis into ribosomal proteins S6 (D41C), S11 (E75C), and L9 (N11C... then associated to create two kinds of ...

Source: Joseph, Simpson - Department of Chemistry and Biochemistry, University of California at San Diego

Collection: Biology and Medicine ; Chemistry

Summary:  2 S17 L7a S5 L11 S21 L8 S18 L9 S6 L21 S28 L10a S23 L13 S10 L24 L12 S26 L13a S11 L32 L34 S27 L14 S13... ://www.landesbioscience.com/journals/psb/abstract.php?id=3724 Key worDS notchless, notch, WD repeat protein, ovule, fertilization, ribosomal protein, ribosomal... trans-acting factor, ...

Source: 

Collection: Biology and Medicine

Summary:  factor P10660 IPI00021840 28834 40S ribosomal protein S6 Ribosomal proteins Q9BRU9 IPI00031612 28811... 14692 IPI00006099 146571 Ribosome biogenesis protein BMS1 homolog RNA modifying enzymes and related... protein Ribosomal biogenesis Q14683 IPI00291939 143771 ...

Source: Lamond, Angus I. - Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee

Collection: Biology and Medicine

Summary:  28834 40S ribosomal protein S6 Ribosomal proteins P23821 IPI00013415 22113 40S ribosomal protein S7... Chromatin related factor Q9Y2S6 IPI00006378 7062 HSPC016 protein Chaperone Q9Y6E2 IPI00022305 48360 HSPC028... 4 homolog RNA modifying enzymes ...

Source: Lamond, Angus I. - Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee

Collection: Biology and Medicine

Summary:  in helices H89 and H91 (Fig. S6). Of the 34 eubacterial LSU ribosomal proteins, 11 were found to have... eubacterial SSU proteins, 10 have homologues in the Lmr SSU. These are proteins S5, S6, S8, S9, S11, S12... Structure of a mitochondrial ribosome with minimal RNA ...

Source: Simpson, Larry - Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles

Collection: Biology and Medicine

Summary:  , S6, S12, S18, and S21). Since the modern ribosome can be viewed as a protein-stabilized ribozyme... precursor 21S. The 61S particles were deficient in r-proteins S1, S2, S6, S12, S18, and S21 and contained... ).Theformationofh2wasshowntobeessentialforbinding of proteinsS1,S2, ...

Source: Bedwell, David M. - Department of Microbiology, University of Alabama at Birmingham

Collection: Biology and Medicine

Summary:  ° structure of the whole ribosome from T. thermophilus, T70S6 and the unbound D50S.7 Note that this bridge... Functional aspects of ribosomal architecture: symmetry, chirality and regulationz Raz Zarivach,1 Anat Bashan... , 76100 Rehovot, Israel 2 Max-Planck-Research Unit for Ribosomal ...

Source: Martin, Jan M.L. - Department of Organic Chemistry, Weizmann Institute of Science

Collection: Chemistry

Summary:  , and the -helices are packed on one side. This exact topology is found in S6 (see Fig. 1), and variations containing... ­ / dsRBD 4 C-terminal domain ­ / Gyr B, EF-G S6 T.t. X-ray 1 / RNP domain 5 S7 B.s. and T.t. X-ray 1... to RNA21 pro- vides a model for the interac- tions between ...

Source: Ramakrishnan, Venki - Laboratory of Molecular Biology, MRC

Collection: Biology and Medicine

Summary:  S3 rpS3p 1­240 6-193 rpS9 rpS4p 1­197 6­21; 56­163 rpS2 rpS5p 1­254 75­236 rpS6p rpS5 rpS7p 1­225 40... Small subunit - E, A only rpS1e rpS4e rpS6e rpS8e rpS17e rpS19e 1­144 4­144 rpS24e rpS25e rpS26e rpS31e... Structure Article Comprehensive Molecular Structure of the Eukaryotic ...

Source: Sali, Andrej - Department of Biochemistry and Biophysics, University of California at San Francisco

Collection: Biology and Medicine ; Biotechnology

Summary:  . The small subunit (0.85 MDa) initiates mRNA en- 1999). We placed the structures of S4, S5, S6, S7, S8... at S18 and pointing toward S6.nate their direction, H44, H7, and H16/H17 (Figure 2) run parallel... H20­H24 and pro- teins S6, S8, S11, S15, and S18 (Figure 2). ...

Source: Martin, Jan M.L. - Department of Organic Chemistry, Weizmann Institute of Science

Collection: Chemistry

Summary:  of which show strong constitutive or conditional phenotypes (Figure S6). For example, deletion of PUF6, SAC... Rational Extension of the Ribosome Biogenesis Pathway Using Network-Guided Genetics Zhihua Li1... , Austin, Texas, United States of America Abstract Biogenesis of ribosomes is an essential cellular ...

Source: Bedwell, David M. - Department of Microbiology, University of Alabama at Birmingham

Collection: Biology and Medicine

Summary:  the ms2-RNA concentration distributions. Figure S6 ms2-RNA expression profiles from four single Frag1A... 100 120 140 160 Time after induction (min) [RNA](!M) Figure S6 #12;... was determined by absorbance at 280nm. ms2-RNA ribosome association assay Cells carrying both reporter

Source: Cluzel, Philippe - Department of Molecular and Cell Biology , Harvard University

Collection: Physics ; Biology and Medicine

Summary:  binding proteins (S4, S7, S8, S15, S17, and S20) and mid-binding proteins (S5, S6, S9, S11, S12, S13, S16... assembly, and these residues interact directly with S6 in the 30S subunit (Table 1; Schuwirth et al. 2005... ), supporting the known role of S6 in assembly ...

Source: Bedwell, David M. - Department of Microbiology, University of Alabama at Birmingham

Collection: Biology and Medicine

Summary:  by the RML murine prion strain [33,34]) and the MovS6 system (immortalized neuroglial cells from ovine... in the MovS6-based assay described above but also in vivo in a mouse model previously described (tg338 mice... against mammalian prion in the MovS6-based assays ...

Source: Uppsala Universitet - Department of Population Biology

Collection: Environmental Sciences and Ecology

Summary:  sequence. The profile accurately predicts position-dependent ribosomal density along yeast genes... . These data suggest that transla- tion speed and, as a consequence, ribosomal density are encoded by coding... stage of translation initiation, forming an optimal and robust means to reduce ribosomal traffic jams

Source: Tuller, Tamir - School of Computer Science, Tel Aviv University

Collection: Computer Technologies and Information Sciences

Summary:  -BP1 phosphorylation is mediated by the FRAP-p70s6k pathway and is independent of mitogen... RNA and ribosomes to focal adhesions Marina E. Chicurel*, Robert H. Singer, Christian J. Meyer* & Donald E. Ingber... -resolution in situ hybridization revealed that mRNA and ribosomes rapidly and specifically ...

Source: Ingber, Donald E. - Vascular Biology Program, Harvard University; Singer, Robert H. - Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Yeshiva University

Collection: Biology and Medicine

Summary:  of the ribosomal protein S6 from Thermus thermophilus. EMBO J. 13, 1249­1254. 10. Jaishree, T.N., Ramakrishnan, V... proteins S6, S8, S15 and S18 with the central domain of 16S ribosomal RNA from Escherichia coli. J. Mol... ). Interaction of ...

Source: Ramakrishnan, Venki - Laboratory of Molecular Biology, MRC

Collection: Biology and Medicine

Summary:  features (listed in Supplementary Table S6), as well as the extent of evolu- tionary conservation for both... not incorporate specificity among the computed parameters. The selected features (Supplementary Table S6) were... Structural signatures of antibiotic binding sites on the ribosome ...

Source: Mandel-Gutfreund, Yael - Department of Biology, Technion, Israel Institute of Technology

Collection: Biology and Medicine

Summary:  -terminal fusion constructs for highest in vivo activity (see Figs. S4, S5, and S6 and SI Materials and Methods... with the wild-type strain in amino acid rich buffers (Fig. S6 in the SI Appendix). Furthermore, its recovery... is induced by overload of selected amino acids (Fig. ...

Source: Dekker, Nynke - Kavli Institute of Nanoscience, Technische Universiteit Delft

Collection: Biology and Medicine

Summary:  of the C-terminal fusion constructs for highest in vivo activity (see Figs. S4, S5, and S6 and SI Materials... as compared with the wild-type strain in amino acid rich buffers (Fig. S6 in the SI Appendix). Furthermore... is induced by overload of selected amino acids (Fig. ...

Source: Ovryn, Ben - Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Yeshiva University

Collection: Biology and Medicine

Summary:  environments (Bremer and Dennis, 1996; Figure S6). SPR concentration (MIC) TMPconcentration(MIC) 6 0 0.5 1 0 1... inhibitors. Here, we show that this suppression results from nonoptimal regulation of ribosomal genes... that ribosomal genes are not directly regulated by DNA stress, leading to an imbal- ance ...

Source: Kishony, Roy - Department of Systems Biology, Harvard University

Collection: Environmental Sciences and Ecology ; Biology and Medicine

Summary:  S6). The observed power-law tail is similar to that found in microarray studies that measured... activities is found during exponential growth when cells are in balanced growth (Methods and Figs S5, S6... , is observed during well-aerated balanced growth as well (Fig ...

Source: Alon, Uri - Departments of Molecular Cell Biology & Physics of Complex Systems, Weizmann Institute of Science

Collection: Biology and Medicine

Summary:  of the ribosomal protein S6 from Thermus thermophilus. EMBO J. 13, 1249­1254. 7. Nikonov, S., et al., & Liljas, A... The structure of ribosomal protein S7 at 1.9 Å resolution reveals a b-hairpin motif that binds... : Ribosomal protein S7, a crucial RNA-binding component of the ...

Source: Ramakrishnan, Venki - Laboratory of Molecular Biology, MRC

Collection: Biology and Medicine

Summary:  and Ribosome Biogenesis in Saccharomyces cerevisiae Jesse W. Loar,* Robert M. Seiser,,1 Alexandra E. Sundberg... August 30, 2004 ABSTRACT In the yeast S. cerevisiae, ribosome assembly is linked to environmental... conditions by the coordinate transcrip- tional regulation of genes required for ribosome biogenesis

Source: Lycan, Deborah E. - Department of Biology, Lewis and Clark College

Collection: Biology and Medicine

Summary:  and Ribosome Biogenesis in Saccharomyces cerevisiae Jesse W. Loar,* Robert M. Seiser,,1 Alexandra E. Sundberg... August 30, 2004 ABSTRACT In the yeast S. cerevisiae, ribosome assembly is linked to environmental... conditions by the coordinate transcrip- tional regulation of genes required for ribosome biogenesis

Source: Craig, Elizabeth A - Department of Biochemistry, University of Wisconsin at Madison

Collection: Biology and Medicine

Summary:  initially organizes a subdomain that includes proteins S20, S16, S15, S6 and S18 (Nowotny and Nierhaus, 1988... The EMBO Journal Vol.17 No.16 pp.4545­4558, 1998 The crystal structure of ribosomal protein S4... .ramakrishnan@m.cc.utah.edu or draper@jhunix.hcf.jhu.edu We report the 1.7 Å crystal structure of ...

Source: Ramakrishnan, Venki - Laboratory of Molecular Biology, MRC

Collection: Biology and Medicine

Summary:  %, with their corresponding protein. Peptide Protein AEVSGPIPLPTDRS 30S ribosomal protein S10 AEVSGPIPLPTDRSVY 30S ribosomal... protein S10 VSGPIPLPTDRS 30S ribosomal protein S10 TNAEVSGPIPLPTDR 30S ribosomal protein S10 AEVESFQLDH 30... S ribosomal protein S10 EGISTDPYERKVI 30S ...

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  crystals of the small subunit from Thermus thermophilus (T30S) [6,7] and only recently we crystallized... Science Publishers Ltd. High-Resolution Structures of Large Ribosomal Subunits from Mesophilic Eubacteria... . of Science, Rehovot, Israel and Max-Planck- Research Unit for Ribosomal Structure, Hamburg ...

Source: Martin, Jan M.L. - Department of Organic Chemistry, Weizmann Institute of Science

Collection: Chemistry

Summary:  ). The pivot point is marked by a red dot. A- P-, and E tRNA were docked from the structure of T70S.6... Ribosomal Crystallography: Peptide Bond Formation and Its Inhibition Anat Bashan1 Raz Zarivach1... for Ribosomal Structure, 22603 Hamburg, Germany 3 FB Biologie, Chemie, Pharmazie, Frei Uni. Berlin, 14195 ...

Source: Martin, Jan M.L. - Department of Organic Chemistry, Weizmann Institute of Science

Collection: Chemistry

Summary:  , partial (36%) CN468730 TC69 40S ribosomal protein S6. fIctalurus punctatus;g, partial (66%) CN468833 CN... arbp-prov protein fXenopus laevis;g, complete CN468922 TC69 40S ribosomal protein S6. f... Ictalurus punctatus;g, partial (66%) CN469397 TC69 40S ...

Source: Hofmann, Hans A. - Section for Integrative Biology, Division of Biological Sciences, University of Texas at Austin; Needleman, Daniel - School of Engineering and Applied Sciences, Harvard University

Collection: Biology and Medicine

Summary:  in ribosome occupancy; Figure S6 and Text S3). These results are consistent with the effects on ribosome... (Spearman r = 0.45, p,10225 ) (Figure S6A), although many mRNAs appeared to differentially change in either... of mRNA targets for which the translational ...

Source: Herschlag, Dan - Department of Biochemistry, Stanford University

Collection: Biology and Medicine

Summary:  Dulin Observation de l'activité traductionnelle d'un ribosome unique par microscopie de uorescence... laboratoire à tenter d'observer l'activité de nos coriaces ribosomes. Il a toujours répondu présent à la... travaux. Grâce à eux, j'ai beaucoup appris sur le ribosome et sur comment réaliser des expériences de

Source: Ecole Polytechnique, Centre de mathématiques

Collection: Mathematics

Summary:  processing speed. In an NGD inactivation context, Rio1p­TAP level is not modified (Supplementary Figure S6... Immature small ribosomal subunits can engage in translation initiation in Saccharomyces cerevisiae... , in Saccharomyces cerevisiae, immature 40S ribosomal subunits are not competent for translation ...

Source: Bedwell, David M. - Department of Microbiology, University of Alabama at Birmingham

Collection: Biology and Medicine

Summary:  distinct shapes, such as S2, S6, al., 1999; Gabashvili et al., 2000; Yusupov et al., 2001). For each bridge... , and is surrounded by S7, S11, S18 and helices 23, 24, 28, and 45 of the 16S rRNA, becomes arrangement comprises S6... and shaded in red. X marks regions of close contact between proteins ...

Source: Sali, Andrej - Department of Biochemistry and Biophysics, University of California at San Francisco

Collection: Biology and Medicine ; Biotechnology

Summary:  ., Stout, C. D. & Williamson, J. R. Structure of the S15,S6,S18-rRNA complex: assembly of the 30S ribosome... . Crystal structure of the ribosomal protein S6 from Thermus thermophilus. EMBO J. 13, 1249±1254 (1994). 42... ®gures is the same as in Figs 3±6. a, The ...

Source: Ramakrishnan, Venki - Laboratory of Molecular Biology, MRC

Collection: Biology and Medicine

Summary:  the polysomal distribution of SUT1 (YGL162W) mRNA before and after osmotic stress (Figure S6). Indeed... %, providing further evidence that this message is subject to translational regulation (Figure S6... (Figures 4 and S6). Instead, our approach allows several ...

Source: 

Collection: Biology and Medicine

Summary:  subunits (Figure S6A). Therefore, these findings show an important role for RPL38 in facilitating 80S... and Figure S6B) (Simeone et al., 1990, 1991). This localization is similar to other RPs, such as RPL4... belonging to the large subunit. Data are presented as the average ± SEM. See also Figure ...

Source: Bedwell, David M. - Department of Microbiology, University of Alabama at Birmingham

Collection: Biology and Medicine

Summary:  Dynamics Inherent in Helix 27 from Escherichia coli 16S Ribosomal RNA John A. H. Hoerter, Meredith... suggested that the highly conserved Escherichia coli 16S ribosomal RNA helix 27 (H27) adopts two alternative... Cl). UV irradiation assays conducted under conditions analogous to those used for assays of ribosomal

Source: Walter, Nils G. - Department of Chemistry, University of Michigan

Collection: Chemistry ; Biotechnology ; Biology and Medicine

Summary:  57619 3 UTR 40S ribosomal protein S6 (Nicotiana tabacum) T72938 3 UTR Ribosomal protein L10 R02593 3 UTR... , as demonstrated for the ribosomal proteins. Clustering also separated cancerous from noncancerous tissue and cell... expression in these tissues. It is demonstrated, for the case ...

Source: Alon, Uri - Departments of Molecular Cell Biology & Physics of Complex Systems, Weizmann Institute of Science

Collection: Biology and Medicine

Summary:  of these strains (Table S6) we derived the genetic Table 2. CGGR variables, parameters and constants. Genetic... packed with ribosomes and proteins. Assuming that under such overcrowded conditions prolonged diffusion... of America Introduction The rRNA (rrn) operons of E. coli have the important role of determining ...

Source: Tlusty, Tsvi - Department of Physics of Complex Systems, Weizmann Institute of Science

Collection: Physics

Summary:  activation by the ribosome Andrew J. Adamczyk and Arieh Warshel1 Department of Chemistry, 418 Seeley G. Mudd... -tRNA delivery to the ribosome is energized by the GTPase reaction of the elongation factor Tu (EF-Tu). Advances... in the elucidation of the structure of the EF-Tu/ribosome complex provide the rare opportunity of ...

Source: Barton, Paul I. - Department of Chemical Engineering, Massachusetts Institute of Technology (MIT)

Collection: Engineering

Summary:  of limited res- olution, correct side chain rotamers were in some instances difficult to ascertain (Figure S6... the TF:S7 Complex TF within the complex comprises three domains (Figures 3A­3C and S6­S8), each... S subunit S1,a S10, S14, S19, S21 S2,a S3, S5,a S7,a S9, S11 S4, ...

Source: Hendrickson, Wayne A. - Department of Biochemistry and Molecular Biophysics, Columbia University

Collection: Biology and Medicine

Summary:  on the top of the sucrose gradient, therefore, they are ribosome free (Fig. S6b). Ribosome recognition... ! mutants (Fig. 3c and Fig. S6b, respectively). These data strongly indicate that decapping and 5... since they persist in dom34! mutants (Fig. ...

Source: Baker, Kristian E. - Center for RNA Molecular Biology, Case Western Reserve University

Collection: Biology and Medicine

Summary:  in the head; S6, S11, and S18 in the platform; and S4, S5, S8, and S16 in the body can be seen... . The platform of the 30S subunit, around proteins S11, S6, and S15, contacts the 50S subunit near protein L2... distinct contacts with 16S rRNA (B7b), at helices 23 and 24; L2 is also very close to protein ...

Source: Economou, Tassos - Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology, Hellas

Collection: Biology and Medicine

Summary:  structure (S6), a homology model for ribosomal protein L11 and the L11 binding region of 23S RNA was derived... (blue) with its conformation in the empty E. coli ribosome (red, from ref. S6). Domain 1 of RF2 consists... to the structure of an empty ...

Source: Ramakrishnan, Venki - Laboratory of Molecular Biology, MRC

Collection: Biology and Medicine

Summary:  to the compound, as evidenced by the increase in apparent IC50,suggests that between Akt/ PKB and S6 ribosomal... and the phosphorylation levels of Akt/PKB and S6 ribosomal protein were assessed with antibodies specific for Ser473... -phospho-Akt (blue) and ...

Source: Boguski, Mark S. - Center for Biomedical Informatics, Harvard University

Collection: Mathematics ; Biology and Medicine

Summary:  Ribosome The blueprint of life embedded in the genomic DNA, is first transcribed into mRNA, which... is then translated into protein. In cells, the organelle for protein synthesis is the ribosome, which is comprised... of two subunits. The mRNA is threaded through the small subunit of the ribosome and tRNA binds via base

Source: Wechsler, Risa H. - Kavli Institute for Particle Astrophysics and Cosmology, Stanford Linear Accelerator Center

Collection: Physics

Summary:  COMMUNICATION Conformational Changes in the Ribosome Induced by Translational Miscoding Agents Olga... , 9500 Gilman Drive, La Jolla CA 92093-0314, USA Ribosomes are dynamic complexes responsible... are a group of miscod- ing agents that bind to the ribosome and reduce the ®delity of trans- lation. Previous

Source: Joseph, Simpson - Department of Chemistry and Biochemistry, University of California at San Diego

Collection: Biology and Medicine ; Chemistry

Summary:  TABLE S6 Transcriptional protein-interaction mediated regulatory loop clusters with the topology... control 1, 6, 38, 47, 50, 56, 73, 78, 79 Transcription activation 31 Ribosomal proteins 54 Protein binding... complex P-value 1 RAP1,FHL1 27 0.79 Ribosomal large subunit 8 × 10-12 ...

Source: 

Collection: Physics ; Mathematics ; Biotechnology

Summary:  against ribosomal protein S6 or a mono- clonal antibody against Mengo virus 2A. (B) Samples from... factor), 2A, or S6 (integral ribosome pro- tein). Only the portions of the blots relative... has been described previously (1). Polyclonal antibodies against ...

Source: Palmenberg, Ann C.- Institute for Molecular Virology & Department of Biochemistry, University of Wisconsin at Madison

Collection: Biology and Medicine

Summary:  ; Makkerh et al., 1996). However, the three NLSs of human S6 do fit the tetrapeptide consensusribosomal... Pathway Used by Ribosomal Proteins Michael P. Rout, Gu¨ nter Blobel, of NLSs (Fischer et al., 1991; Garcia... are ribosomal proteins, which must be A detailed analysis of the composition of a highly en- ...

Source: Rout, Michael P. - Laboratory of Cellular and Structural Biology, Rockefeller University

Collection: Biology and Medicine

Summary:  IF4E) nor 40S ribosomal subunits (as exemplified by S6 ribosomal protein) were co-depleted with PABP... ) using antibodies against eIF4GI, PABP, eIF4AI, S6 ribosomal protein, and eIF4E as indicated. Lanes 3... E were described (Khaleghpour et al, ...

Source: Bedwell, David M. - Department of Microbiology, University of Alabama at Birmingham

Collection: Biology and Medicine

Summary:  .sciencemag.org/cgi/content/full/310/5753/1513/ DC1 Materials and Methods Figs. S1 to S6 Movie S1 17 August 2005; accepted 1 November... that controls assembly of 40S ribosomal subunits on mes- senger RNAs (mRNAs) bearing either a 5¶-cap... or an internal ribosome entry site (IRES). Cryo­electron microscopy ...

Source: Doudna, Jennifer A. - Department of Molecular and Cell Biology, University of California at Berkeley

Collection: Biology and Medicine

Summary:  Molecular Biology Organization The EMBO Journal VOL 27 | NO 19 | 2008 2537 #12;Figure S6); LS-2 where... , two landing sites were observed, GGA48­50 and GGG9­11 (Supplementary Figure S6 and Figure 7B). Landing... , UT, USA Half the ribosomes translating the mRNA for phage T4 gene 60 ...

Source: Bedwell, David M. - Department of Microbiology, University of Alabama at Birmingham

Collection: Biology and Medicine

Summary:  Polar bears, antibiotics, and the evolving ribosome Nobel Lecture Ada Yonath Angew Chem Int Ed Engl... Lectures DOI: 10.1002/anie.201001297 Hibernating Bears, Antibiotics, and the Evolving Ribosome (Nobel... Lecture)** Ada Yonath* antibiotics · Nobel lecture · protein synthesis · ribosomes 1. Introduction

Source: Martin, Jan M.L. - Department of Organic Chemistry, Weizmann Institute of Science

Collection: Chemistry

Summary:  gene resulted in the same topology as in Fig. 1 (Fig. S6). Second, we explored the effect of using... such as ribosomal RNA (rRNA). However, phylogenies reconstructed from single or a small number of genes are hampered... of the Metazoa based on morphological and 18S ribosomal DNA evidence. Cladistics 14, ...

Source: Ecole Polytechnique, Centre de mathématiques

Collection: Mathematics

Summary:  /MS in the excised bands 1­22 (see Figure 3D) Band Ribosomal subunits 1 L3 2 L1 3 L5 4 S2, S3A, S4 5 S6 6 L7 7 L8 8 S... Sequence and structure evolved separately in a ribosomal ubiquitin variant Andre´ Catic1,5,7 , Zhen... of three precursor proteins, two of which are fusions to the ...

Source: Ratner, Daniel M. - Department of Bioengineering, University of Washington at Seattle

Collection: Biology and Medicine ; Biotechnology

Summary:  . 4 A and B and Fig. S6). The universally conserved GGQ motif (residues 251­253 in T. thermophilus RF2... - plex formed by the Thermus thermophilus 70S ribosome bound with release factor RF2, in response... of the RF2 complex supports our previous proposal that conformational changes in the ribosome in ...

Source: Scott, William - Department of Chemistry and Biochemistry, University of California at Santa Cruz

Collection: Chemistry

Summary:  and trehalose biosynthesis). In contrast, 95% of ribosomal proteins, as well as tRNA syn- thetases... . It also highlights the induction of heat shock proteins and the repression of ribosome biosynthesis... ribosomal subunits, nucleolar rRNA processing and translational elongation is faithfully reconstructed

Source: Breitling, Rainer - Groningen Bioinformatics Centre, Rijksuniversiteit Groningen

Collection: Biology and Medicine ; Biotechnology

Summary:  particle and the ribosome Ted Powers and Peter Walter Background: The first step in the co... sequences by the 54 kDa subunit of the signal recognition particle (SRP) as they emerge from the ribosome... to the fidelity of targeting by modulating interactions that occur between the ribosome­nascent chain complex

Source: Walter, Peter - Department of Biochemistry and Biophysics, University of California at San Francisco

Collection: Biology and Medicine

Summary:  S ribosomes from Bacillus stearothermophilus J. Pie&e, T. Arad*, H.S. Gewitz, A. Yonath* and H.G. Wittmann Max... of intact 70 S ribosomes from Bacillus stearothermophilus have been obtained in vitro using salt... features to 42 A resolution. Ribosome Two-dimensional sheet 70 S ribosomal particle ...

Source: Martin, Jan M.L. - Department of Organic Chemistry, Weizmann Institute of Science

Collection: Chemistry

Summary:  - lian cell size is controlled by mTOR and its downstream targets S6K1 and 4EBP1/eIF4E. Genes Dev. 16... Reserved. Ribosomal Protein L11 Negatively Regulates Oncoprotein MDM2 and Mediates a p53-Dependent... Ribosomal-Stress Checkpoint Pathway Yanping Zhang,1 * Gabrielle White Wolf,2 Krishna Bhat,1 Aiwen Jin,1

Source: Xiong, Yue - Lineberger Comprehensive Cancer Center & Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill

Collection: Biology and Medicine

Summary:  supercomplex, the translasome, which contains elongation factors, tRNA-synthetases, 40S and 60S ribosomal... proteins, chaperones, and the proteasome. eIF3 also associates with ribosome biogenesis factors... interactome and suggest that factors involved in translation initiation, ribosome biogenesis, translation

Source: Wolf, Dieter A.- Burnham Institute for Medical Research

Collection: Biology and Medicine

Summary:  Dispatch R731 Ribosomes: Cashing in on crystals Jennifer A. Doudna Structures of the ribosome... in the intact ribosome. Address: Howard Hughes Medical Institute, Department of Molecular Biophysics... . The ribosome has long been subject to intense scrutiny by molecular and cell biologists because of its ...

Source: Doudna, Jennifer A. - Department of Molecular and Cell Biology, University of California at Berkeley

Collection: Biology and Medicine

Summary:  BiP Modulates the Affinity of Its Co-chaperone ERj1 for Ribosomes*S Received for publication,May 11... of Freiburg, 79104 Freiburg, Germany Ribosomes synthesizing secretory and membrane proteins are bound... to the endoplasmic reticulum (ER) membrane and attach to ribosome-associated membrane proteins such as the Sec61

Source: Snapp, Erik Lee - Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Yeshiva University

Collection: Biology and Medicine

Summary:  similarities of ribosome- associated chaperones from Saccharomyces cerevisiae and Escherichia coli Thomas Rauch... , University of Wisconsin- Madison, 433 Babcock Drive, Madison, WI 53706, USA. Summary Ribosome... and eukaryotic systems. However, these ribosome-associated chaperones share no sequence similarity: bacterial

Source: Craig, Elizabeth A - Department of Biochemistry, University of Wisconsin at Madison

Collection: Biology and Medicine

Summary:  Abstract. Ribosomes synthesizing nascent secretory proteins are targeted to the membrane by the signal... the ribosome. SRP arrests further elongation, causing ribosomes to stack behind the arrested ribosome. Upon... interaction of SRP with its receptor on the ER membrane, the translation arrest is released ...

Source: Walter, Peter - Department of Biochemistry and Biophysics, University of California at San Francisco

Collection: Biology and Medicine

Summary:  Ribosome-tethered molecular chaperones: the first line of defense against protein misfolding... revealed the presence of ribosome- associated molecular chaperones, thought to be the first line of defense... against protein aggregation as translating polypeptides emerge from the ribosome. However, structurally

Source: Craig, Elizabeth A - Department of Biochemistry, University of Wisconsin at Madison

Collection: Biology and Medicine

Summary:  Complementary roles of initiation factor 1 and ribosome recycling factor in 70S ribosome splitting... Biology, BMC, Uppsala University, Uppsala, Sweden We demonstrate that ribosomes containing a messenger RNA... (IF1) and 3 (IF3), but slowly split by ribosome recycling factor (RRF) and elongation factor G (EF

Source: Joseph, Simpson - Department of Chemistry and Biochemistry, University of California at San Diego

Collection: Biology and Medicine ; Chemistry

Summary:  to the phosphorylation of eIF4E- binding proteins (4E-BPs) and of the S6 ribosomal pro- tein (S6) by the ribosomal... to the phosphorylated form of eIF2 and the S6 ribosomal protein (bottom). Total eIF2 ...

Source: Paris-Sud XI, Université de - Institut d'Optique, Laboratoire Charles-Fabry, Groupe d'Optique Atomique

Collection: Physics

Summary:  ribosome. Philos Trans R Soc Lond B Biol Sci 366, 2972-2978. Author's personal copy #12;Research A vestige... of a prebiotic bonding machine is functioning within the contemporary ribosome Miri Krupkin, Donna Matzov, Hua... , it is suggested that a universal symmetrical region identified within all contemporary ribosomes ...

Source: Yonath, Ada E. - Department of Structural Biology, Weizmann Institute of Science

Collection: Biology and Medicine

Summary:  -line version of this article (available at http://www.jbc.org) contains supplemental Table 1 and Figs. S1­S6. 1... are conserved. However, there are several distinct differences between them (supple- mental Fig. S6). First... in RelE and aRelE than in YoeB (supplemental Fig. ...

Source: Ikura, Mitsuhiko - Division of Molecular and Structural Biology, University of Toronto

Collection: Biotechnology ; Biology and Medicine

Summary:  and ribosome concentrations as a function of the rrn operon copy number 24 Figure S6 Breakdown of the ribosome... doub/h, 37°C 9 Table S5 Genetic parameters for E. coli growing at 2 doub/h, 37°C 12 Table S6 Lineage... rate (Eq. ...

Source: Tlusty, Tsvi - Department of Physics of Complex Systems, Weizmann Institute of Science

Collection: Physics

Summary:  immunoprecipitation Category Proteins identified by mass spectrometry Small ribosomal subunits S2, S3, S3a, S4, S5, S6... ribosomal protein S6 and PABP. The results show that PYM interacts with the small ribosomal subunit... initiation complexes ...

Source: Dreyfuss, Gideon - Department of Biochemistry and Biophysics, University of Pennsylvania

Collection: Biology and Medicine

Summary:  -cycle arrest caused by depletion of S6 in A549 cells (Fumagalli et al., 2009). Alternately, at least... Molecular Cell Article Ribosomal Protein S7 Is Both a Regulator and a Substrate of MDM2 Yan Zhu,1... .07.014 SUMMARY MDM2 associates with ribosomal protein S7, and this interaction is required to inhibit ...

Source: Bedwell, David M. - Department of Microbiology, University of Alabama at Birmingham

Collection: Biology and Medicine

Summary:  Structure of the S15,S6,S18-rRNA Complex: Assembly of the 30S Ribosome Central Domain Sultan C... , and the ribosomal proteins S15, S6, and S18. S15 binds the ribosomal RNA early in the assembly of the 30S ribosomal... proteins ...

Source: Stout, Charles D. - Department of Molecular Biology, Scripps Research Institute

Collection: Biology and Medicine

Summary:  The EMBO Journal vol.7 no. 1 1 pp.3559 - 3569, 1988 Ribosome pausing and stacking during... a sensitive assay to determine the distribution oftranslating ribosomes on a mRNA. Using this assay to monitor... ribosome transit on bovine preprolactin mRNA, we have detected four major positions of ribosome pausing

Source: Walter, Peter - Department of Biochemistry and Biophysics, University of California at San Francisco

Collection: Biology and Medicine

Summary:  kinase related to AKT and S6K) that activate expression of tran- scriptional units encoding ribosomal... availability, stress, and growth factors [1]. One well-described downstream target of mTORC1 is the S6 kinase 1... (S6K1) [1]. mTORC1 ...

Source: Sabatini, David M. - Whitehead Institute for Biomedical Research & Department of Biology, Massachusetts Institute of Technology (MIT)

Collection: Biology and Medicine

Summary:  Ribosome's mode of function: myths, facts and recent results Itai Wekselman,a§ Chen Davidovich... December 2008 (www.interscience.com) DOI 10.1002/psc.1077 Ribosome's mode of function: myths, facts... Bashan,a Rita Berisiob and Ada Yonatha Ribosomes translate the genetic code into proteins in all living

Source: Martin, Jan M.L. - Department of Organic Chemistry, Weizmann Institute of Science

Collection: Chemistry

Summary:  for studying the structure and func- tion of bacterial ribosomes. During the late `nineties significant... for ribosomes and their subunits at a resolution of 10 ­ 15 Å. In collaboration with the cryo-EM group of Marin... -EM envelopes. The cryo-EM structures could also be used by ribosomal crystallographers to phase their ...

Source: Spang, Rainer - Computational Molecular Biology Group, Max-Planck-Institut für molekulare Genetik

Collection: Computer Technologies and Information Sciences ; Biotechnology ; Biology and Medicine