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Sample search results for: a549 cell cultures

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1 Nucleic Acids Research doi:10.1093/nar/gki514
 

Summary:  A549 lung carcinoma cell line was obtained from the American Type Culture Collection (Rockville, MD... and apoptosis in the human A549 tumour cell line. We show here that 12459 induces a delayed apoptosis... that activates the mitochondrial ...

  

Source: Ecole Polytechnique, Centre de mathématiques

 

Collection: Mathematics

 
2 THE EFFECTS OF IN VIVO MECHANICAL FORCES ON MORPHOLOGY, PROLIFERATION AND PROTEIN EXPRESSION OF LUNG CANCER CELLS
 

Summary:  (NSCLC) cell lines. Methods: The epithelial lung carcinoma (A549 ) and a bronchioalveolar (NCI-H358... did not alter the morphology of A549 cells at either time points (not shown). Proliferation: Both cell... 3b). ...

  

Source: Peterson, Blake R. - Department of Medicinal Chemistry, University of Kansas

 

Collection: Chemistry

 
3 BioMed Central Page 1 of 8
 

Summary:  monolayers of SAECs and A549 cells were cultured in Transwell plates and SAECs were allowed to differentiate... mediumFigure 2 Gas phase NO release from stimulated SAECs/A549 cells and total nitrate/nitrite in culture... , *P ...

  

Source: George, Steven C. - Department of Biomedical Engineering, University of California, Irvine

 

Collection: Biology and Medicine

 
4 PrtT-Regulated Proteins Secreted by Aspergillus fumigatus Activate MAPK Signaling in Exposed A549
 

Summary:  Microarray experiments A549 cells were grown at a density of 16106 cells/100 mm dish, and cultured in serum... to the manufacturer's protocols. For RT-PCR, A549 cells were cultured on 10-cm ...

  

Source: Shamir, Ron - School of Computer Science, Tel Aviv University

 

Collection: Computer Technologies and Information Sciences

 
5 An NQO1-and PARP-1-mediated cell death pathway induced in non-small-cell lung cancer cells
 

Summary:  or expressed NQO1 along with A549 NSCLC cells treated with or without dicoumarol, were used to elucidate... -exposed NQO1 A549 cells were resistant (LD50, >40 M) to ROS formation and all cytotoxic effects of -lapachone... ). We then examined the responses of human ...

  

Source: Gao, Jinming - Department of Oncology and Pharmacology, University of Texas Southwestern Medical Center at Dallas

 

Collection: Biology and Medicine

 
6 Group B Streptococcal b-Hemolysin/Cytolysin Promotes Invasion of Human Lung Epithelial Cells and the Release of Interleukin-8
 

Summary:  inva- sion, and IL-8 release assays. Lung epithelial cell culture. A549 cells (American Type Cul- ture... , after transfer to A549 cell tissue culture media under conditions mimicking those used in the ...

  

Source: Doran, Kelly - Department of Biology, San Diego State University

 

Collection: Biology and Medicine

 
7 [CANCER RESEARCH 63, 64886495, October 1, 2003] Human Epithelial Cancers Secrete Immunoglobulin G with Unidentified Specificity
 

Summary:  of Military Medical Science); cervical cancer cell line (HeLa S3), lung cancer cell line (A549), ovarian... . To detect CD19 on cancer cells, cancer cell lines (A549, HT-29 and HeLaS3) and Raji cells were ...

  

Source: Ma, Dalong - Center for Human Disease Genomics, Peking University

 

Collection: Biology and Medicine

 
8 JOURNAL OF CELLULAR PHYSIOLOGY 176:216222 (1998) Primary Cell Cultures From Murine Kidney
 

Summary:  class include A549 artifact of extended cell culture. For example, muta-(Cain et al., 1989), BALB/c 3T3... alkalinization is observed in A549 cells afterwhich grew well in culture. Figure 3 shows micro- the minimal p... from ...

  

Source: Murphy, Robert F. - Department of Biological Sciences & School of Computer Science, Carnegie Mellon University

 

Collection: Biology and Medicine ; Computer Technologies and Information Sciences

 
9 Molecular Biology of the Cell Vol. 9, 26272638, September 1998
 

Summary:  ). Cells and Cell Culture Conditions A549 cells are derived from a human lung adenocarcinoma and were... - function in the ECM, we determined whether latent TGF- 1 interacts with integrins. A549 cells ...

  

Source: Wang, Da-Neng - Skirball Institute of Biomolecular Medicine & Department of Cell Biology, New York University

 

Collection: Biology and Medicine

 
10 BioMed Central Page 1 of 12
 

Summary:  culture and treatment We used human alveolar epithelial (A549) and mono- cyte/macrophage (THP-1) cell... diameter and various elemental compositions on 2 human pulmonary cell lines: A549 and THP-1. A secondary... : ...

  

Source: Ecole Polytechnique, Centre de mathématiques

 

Collection: Mathematics

 
11 Determinants of Cell-and Gene-Specific Transcriptional Regulation by the
 

Summary:  known or potentially glucocorticoid- responsive genes in A549 human lung cells for GR-occupied GREs. We... found that GR was bound in A549 cells predominately near genes responsive to glucocorticoids in those... is a primary determinant of glucocorticoid ...

  

Source: Walter, Peter - Department of Biochemistry and Biophysics, University of California at San Francisco

 

Collection: Biology and Medicine

 
12 TGF-2 reduces nitric oxide synthase mRNA through a ROCK-dependent pathway in airway epithelial cells
 

Summary:  prior to each experiment. A549 cells were cultured with serum-free F-12K medium. Previous studies have... - to threefold in both primary small airway epithelial cells (SAECs) and the A549 cell line. Preincubation... to near ...

  

Source: George, Steven C. - Department of Biomedical Engineering, University of California, Irvine

 

Collection: Biology and Medicine

 
13 BioMed Central Page 1 of 21
 

Summary:  in the supernatants of 16HBE, A549 and HNT primary culture cells was evalu- ated by sandwich-ELISA. As shown in Figure... by human bronchial epithelial 16HBE cells and A549 pneumocyte cells exposed to A. fumigatus... ]. This ...

  

Source: Ecole Polytechnique, Centre de mathématiques

 

Collection: Mathematics

 
14 RESEARCH PAPER Microbioreactor designed for integration with piezoelectric
 

Summary:  cantilevers was verified by culturing A549 lung cancer cells on the cantilever array. The device was first... A549 lung cancer cells were then loaded into culture chamber, and all fluid flow were ceased... and proliferations ...

  

Source: Tang, William C - Department of Electrical and Computer Engineering, University of California, Irvine

 

Collection: Engineering

 
15 Original Article Efficient Derivation of Alveolar Type II Cells
 

Summary:  . To investigate this, we cultured ESCs in conditioned medium from A549 cells, an AEII-like cell line... -expressing cells (standard deviation ¼ 3.8%). Cell heterogeneity in A549 ...

  

Source: Lelkes, Peter I. - School of Biomedical Engineering, Drexel University

 

Collection: Materials Science ; Biology and Medicine

 
16 INTRODUCTION Acute lung injury is characterized by lesions of both lung
 

Summary:  . The A549 alveolar epithelial cell line and primary rat alveolar epithelial cell cultures were used. Cell... alveolar epithelial cells A549 human alveolar epithelial cells (American ...

  

Source: Ecole Polytechnique, Centre de mathématiques

 

Collection: Mathematics

 
17 Synergistic Cytokine-Induced Nitric Oxide Production in Human Alveolar Epithelial Cells
 

Summary:  (Cambridge, MA). Cell Culture Immortal Type II alveolar cell lines of human origin (A549) were obtained from... of A549 cells to a factorial combination of three cyto- kines (IL-1 , TNF- , and IFN- ) and three... IL-1 , TNF- ...

  

Source: George, Steven C. - Department of Biomedical Engineering, University of California, Irvine

 

Collection: Biology and Medicine

 
18 J Toxicol Environ Health A . Author manuscript Adverse effects of industrial multiwalled carbon nanotubes on human
 

Summary:  and crocidolite) and carbon black (CB) nanoparticles, not only in A549 cells, but also on mesothelial cells (MeT5A... ) as well as cellular internalization of CNT were analyzed in the human lung epithelial cell line A549... carbon black (CB) ...

  

Source: Ecole Polytechnique, Centre de mathématiques

 

Collection: Mathematics

 
19 Adverse effects of industrial multi-walled carbon nanotubes on human pulmonary cells.
 

Summary:  (see below). Cell culture and stimulation Human alveolar epithelial cells (A549 cell line, ATCC, France... epithelial cell line A549. MWCNT were dispersed in dipalmitoyl lecithin (DPL), a component of ...

  

Source: Ecole Polytechnique, Centre de mathématiques

 

Collection: Mathematics

 
20 Classification : Original Article VOLTAGE-GATED SODIUM CHANNELS POTENTIATE THE INVASIVE
 

Summary:  , Calu-1 and A549 cell lines are respectively lung adenocarcinomal cells (Gazdar et al., 1980), large... was purchased from Molecular Probes (The Netherlands). Confocal microscopy NL20, A549 and H460 cells were... in the ...

  

Source: Ecole Polytechnique, Centre de mathématiques

 

Collection: Mathematics


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21 Spring of 2004 Brain Tissue Culture Course 625
 

Summary:  Spring of 2004 1 Dandan Sun Brain Tissue Culture Course 625 I. Syllabus January 22: Cell Cultures... (Lecture-3hr) · type of nerve cell cultures · general principles of cell cultures · basic skills... to characterize cell ...

  

Source: Sun, Dandan - Center for Neuroscience, University of Wisconsin at Madison

 

Collection: Biology and Medicine

 
22 Chemistry & Biology, Vol. 10, 881892, September, 2003, 2003 Elsevier Science Ltd. All rights reserved. DOI 10.1016/j.chembiol.2003.08.009 Biological Mechanism Profiling Using an Annotated
 

Summary:  collection, 85 caused a 50% or more reduction in staining of A549 cells with the analysis [28]. We used our... ;Mechanism Profiling with Annotated Compounds 885 Figure 2. 85 ACL Compounds Inhibit A549 Cell Viability... and separates active compounds from all negative ...

  

Source: Stockwell, Brent R. - Department of Biological Sciences, Columbia University

 

Collection: Biology and Medicine

 
23 JOURNAL OF VIROLOGY, May 2006, p. 50215031 Vol. 80, No. 10 0022-538X/06/$08.00 0 doi:10.1128/JVI.80.10.50215031.2006
 

Summary:  in A549 lung alveolar epi- thelial cells demonstrated the biological relevance of these findings to RV... the type I interferon response and status of IRF-3 in infected HeLa and A549 cells. Our results indicate... , 2 mM L-glutamine, and penicillin-streptomycin at ...

  

Source: Gustin, Kurt E. - Department of Microbiology, Molecular Biology and Biochemistry, University of Idaho

 

Collection: Biology and Medicine

 
24 Microarrays of small molecules embedded in biodegradable polymers for use in mammalian
 

Summary:  in a 100 100 10-mm, square tissue-culture dish. Actively growing cells in 25 ml of culture medium (A549... podo- phyllotoxin (PTX) or PAO and cultured A549 cells on it (Fig. 1). Similar to previous ...

  

Source: Sabatini, David M. - Whitehead Institute for Biomedical Research & Department of Biology, Massachusetts Institute of Technology (MIT); Stockwell, Brent R. - Department of Biological Sciences, Columbia University

 

Collection: Biology and Medicine

 
25 YU ET AL. VOL. XXX ' NO. XX ' 000000 ' XXXX www.acsnano.org
 

Summary:  B-enhanced siRNA endosomal escape, A549-Luc cells were seeded in a 35 mm cell culture dish and grown for 24 h... knockdown studies were performed in A549-Luc cells. Cells were seeded into 96- well tissue ...

  

Source: Gao, Jinming - Department of Oncology and Pharmacology, University of Texas Southwestern Medical Center at Dallas

 

Collection: Biology and Medicine

 
26 A haploid genetic screen identifies the major facilitator domain containing 2A (MFSD2A) transporter as a key
 

Summary:  -overexpressing A549 cells in mouse xenograft studies (35). A potential trans- porter function for MFSD2A has... (KD) A549 cells were created (Figs. S2 C and D and S3 C and E show validation of hairpins; see Table S... 1 for shRNA sequences). The single GRP78 or DPAGT1 ...

  

Source: Sabatini, David M. - Whitehead Institute for Biomedical Research & Department of Biology, Massachusetts Institute of Technology (MIT)

 

Collection: Biology and Medicine

 
27 Identification of GAPDH as a protein target of the saframycin antiproliferative agents
 

Summary:  and Methods Cells and Reagents. HeLa-S3 and A549 cells (American Type Culture Collection) were cultured... by filtration through a Bio-spin column (Bio-Rad). Lysate from an A549 tumor-cell line was ...

  

Source: Myers, Andrew G.- Department of Chemistry and Chemical Biology, Harvard University

 

Collection: Chemistry

 
28 Physical plasticity of the nucleus in stem cell differentiation
 

Summary:  '' (average SD). Lamin A/C knockdown Control human A549 epithelial cell Nuclei B D C 1 10 1 10 ,ecnailpmo... CraelcuN)t(JaPk[1- ] t [s] Stem Cell Nuclei primary Fibroblast A549 epithelial cell A549 lamin A/C ...

  

Source: Discher, Dennis - Department of Bioengineering, University of Pennsylvania; Sniadecki, Nathan J. - Department of Mechanical Engineering, University of Washington at Seattle

 

Collection: Biology and Medicine ; Engineering

 
29 The Heparan Sulfate Proteoglycan GPC3 Is a Potential Lung Tumor Suppressor
 

Summary:  (22, 23). Two lung carcinoma cell lines (A549 and NCI-H460) were incubated with viral supernatant... with highest GPC3 expression as detected by immunoblot (clone 1 and clone 5 in A549 and H460 cells... (GFP alone) were controls for all experiments. ...

  

Source: Tian, Weidong - Institute of Biochemistry and Cell Biology, Shanghai Institute of Biological Sciences

 

Collection: Biology and Medicine

 
30 JOURNAL OF VIROLOGY, Nov. 2008, p. 1100911015 Vol. 82, No. 22 0022-538X/08/$08.00 0 doi:10.1128/JVI.01608-08
 

Summary:  nor- mal mode of miRNA action), we extracted RNA from Huh7 and A549 cell cultures infected for 1, 2... of firefly luciferase (FFluc) cDNA in the pcDNA3 (Invitro- gen)-derived plasmid psiRNALUC. A549 or Huh7 cells... cell ...

  

Source: Hemminki, Akseli - Department of Biosciences, University of Helsinki

 

Collection: Biology and Medicine

 
31 Mechanisms of Synergistic Cytokine-Induced Nitric Oxide Production in Human Alveolar Epithelial Cells
 

Summary:  NOS protein, and NO production in A549 monolayers (human alveolar epithelial cell line) in response... of the synergistic NO pro- duction by A549 cells (human alveolar epithelial cell line) to TNF- , IL-1 , and INF- (12... underlying the ...

  

Source: George, Steven C. - Department of Biomedical Engineering, University of California, Irvine

 

Collection: Biology and Medicine

 
32 Systematic discovery of multicomponent therapeutics Alexis A. Borisy, Peter J. Elliott, Nicole W. Hurst, Margaret S. Lee, Joseph Lehar, E. Roydon Price, George Serbedzija,
 

Summary:  of cytokine production. A549 Tumor Cell in Vitro BrdUrd Incorporation Assay. Cell cultures composed of A549... and pentamidine for 90 h and viability was measured by BrdUrd incorporation. A549 Tumor ...

  

Source: Stockwell, Brent R. - Department of Biological Sciences, Columbia University

 

Collection: Biology and Medicine

 
33 From Biology to DiscoveryTM LipodinAbTM
 

Summary:  Source 3T6 Embryonic fibroblasts Mouse A549 Nonsmall cell lung carcinoma Human B16F10 Melanoma... minutes. The mixture is then added to the cells in culture, the lipidantibody complexes... at a density of 2 to 5 x 105 cells/ml. Table 1: ...

  

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

 

Collection: Biotechnology ; Biology and Medicine

 
34 From Biology to DiscoveryTM LipodinProTM
 

Summary:  A549 Nonsmall cell lung carcinoma Human B16F10 Melanoma Mouse BEAS2B Bronchial epithelial cells... for 10 minutes. The mixture is then added to the cells in culture, the lipidprotein... experiment at a density of 2 to 5 x 105 ...

  

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

 

Collection: Biotechnology ; Biology and Medicine

 
35 Cellular and Pharmacological Selectivity of the Peroxisome Proliferator-Activated Receptor-/ Antagonist GSK3787S
 

Summary:  Culture. The human hepatocarcinoma cell lines HepG2 and Huh7, lung adenocarcinoma cell lines A549 and H... procedures: HepG2, Huh7, A431, and MCF7 cells were cultured in DMEM; A549 ...

  

Source: Omiecinski, Curtis - Department of Veterinary and Biomedical Sciences, Pennsylvania State University

 

Collection: Biology and Medicine

 
36 ForPeerReview High Kallikrein-Related Peptidase 6 in Non-Small Cell Lung
 

Summary:  in the recommended culture medium. The A549 Flp-In cell line was derived from A549 cells genetically modified in our... antibody. Equal volumes of cell culture supernatant were loaded onto 12% SDS-PAGE ...

  

Source: Ecole Polytechnique, Centre de mathématiques

 

Collection: Mathematics

 
37 Differential regulation of RANTES and IL-8 expression in lung adenocarcinoma cells
 

Summary:  (PKC)/ on the expression of RANTES and IL-8 in A549 lung adenocarcinoma cells. TNF- is a major mediator... into the mechanism underlying the differential regulation of RANTES and IL-8 in A549 cells. Materials and Methods... Macromoléculaire, Montpellier, France) ...

  

Source: Ecole Polytechnique, Centre de mathématiques

 

Collection: Mathematics

 
38 BioMed Central Page 1 of 9
 

Summary:  such as the A549 cell line and human umbilical vein endothelial cells for which phago- cytosis rates of 30... conidia on cultured A549 pneumocytes. J Infect Dis 1997, 175:146-150. 10. Wasylnka JA, Moore MM: Uptake... -15]. The HNEC ...

  

Source: Ecole Polytechnique, Centre de mathématiques

 

Collection: Mathematics

 
39 Fluorescent Peptide Biosensor for Probing the Relative Abundance of Cyclin-Dependent Kinases in Living Cells
 

Summary:  purchased from Invitrogen. HeLa, HS68, A549, MCF-7 and U2OS cells were obtained from ATCC and were cultured... at wild-type levels, or as full-length proteins, such as lung cancer A549 and breast cancer MCF-7 cells... or ...

  

Source: Paris-Sud XI, Université de - Institut d'Optique, Laboratoire Charles-Fabry, Groupe d'Optique Atomique

 

Collection: Physics

 
40 Supplementary Information A novel sialyltransferase inhibitor suppresses FAK/paxillin and
 

Summary:  3x104 of the CL1-0, CL1-5, A549, and H1299 lung cancer cells were seeded on 12-well culture dishes... knockdown or ectopic expression of ST3Gal I or ST6Gal I for 48 hr. A549 cells (2x105 ) were then seeded onto... treatment with Lith-O-Asp, the ...

  

Source: Gleeson, Joseph G. - Department of Neurosciences, University of California at San Diego

 

Collection: Biology and Medicine


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41 Loss of -Dystroglycan Laminin Binding in Epithelium-derived Cancers Is Caused by Silencing
 

Summary:  -essential amino acids and insulin (5 g/ml; Sigma Chemical). A549, H1299, and H2030 cells were cultured in RPMI... ) and a low metastasis lung cancer cell line (A549). MDA-MB-231, H1299, H2030, and HeLa cells were originally... ...

  

Source: Campbell, Kevin P. - Department of Physiology and Biophysics, University of Iowa

 

Collection: Biology and Medicine

 
42 Expression of Janus Kinase 3 in Human Endothelial and Other Non-lymphoid and Non-myeloid Cells*
 

Summary:  levels in human umbilical vein endothelial cells (HUVEC), human aortic smooth muscle cells (HASMC), A549... cell line U937, the T cell line MT2, HASMC, A549, and HMEC-1 cells (data not shown). PCR analysis ...

  

Source: Bach, Erika - Department of Pharmacology, New York University

 

Collection: Biology and Medicine

 
43 BIOLOGY 4201 -ANIMAL CELL CULTURE: METHODS & APPLICATIONS January 11, 2008
 

Summary:  BIOLOGY 4201 - ANIMAL CELL CULTURE: METHODS & APPLICATIONS January 11, 2008 Instructor: Dr. J... : There is no single textbook for this course. A general reference for animal cell culture methodology is Culture... lab exercises designed to integrate the study of tissue ...

  

Source: Carleton University, Geomatics and Landscape Ecology Research Laboratory

 

Collection: Environmental Sciences and Ecology ; Geosciences

 
44 2011;71:473-483. Published OnlineFirst January 11, 2011.Cancer Res Jia-Yang Chen, Yen-An Tang, Sin-Ming Huang, et al.
 

Summary:  lung cancer cell lines, H1299 and A549, were obtained from the American Type Culture Collection... -2,3-sialylation on cell surface. AL10 suppresses lung metastasis of A549 in tail... the ST3Gal I protein level by 20% to 25% in H1299, CL1-5, and ...

  

Source: Gleeson, Joseph G. - Department of Neurosciences, University of California at San Diego

 

Collection: Biology and Medicine

 
45 Algorithmic guided screening of drug combinations of arbitrary size for activity against cancer cells
 

Summary:  and the number of drugs. Using a WST-1 assay on the A549 cell line, through MACS, we screened 72 combinations... in A549, the one cell line in which this was evaluated. These results suggest that when guided by MACS... potentiated inhibition of ...

  

Source: Popova, Elmira - Department of Mechanical Engineering, University of Texas at Austin

 

Collection: Engineering

 
46 L E T T E R S NATURE CELL BIOLOGY VOLUME 6 | NUMBER 2 | FEBRUARY 2004 129
 

Summary:  expression in RASSF1A-deficient A549 lung cancer cells with a retroviral vector for this protein (Fig. 4a... in these cells (Fig. 4a). All of these modified A549 and HeLa cells under- went normal mitotic arrest in response... immunoprecipitates ...

  

Source: Kirschner, Marc W. - Department of Systems Biology, Harvard University

 

Collection: Biology and Medicine

 
47 Intracellular scFvs against the viral E6 oncoprotein provoke apoptosis in human papillomavirus-positive cancer cells
 

Summary:  two HPV16- transformed cell lines, SiHa and CaSki, and two control cell lines, A549 and HeLa. He... 177P) and negative control cell lines (HeLa and A549, Fig. 3A). This was very likely due to the toxic... -transduced cells (nt) ...

  

Source: Ecole Polytechnique, Centre de mathématiques

 

Collection: Mathematics

 
48 Hyperinduction of Cyclooxygenase-2Mediated Proinflammatory Cascade: A Mechanism for the
 

Summary:  . Influenza virus infection of A549. A549 cells were obtained from ATCC and maintained in culture using... (uninfected) A549 cells. The A549 ...

  

Source: Ecole Polytechnique, Centre de mathématiques

 

Collection: Mathematics

 
49 Growth-Inhibitory and Tumor-Suppressive Functions of p53 Depend
 

Summary:  in 5% CO2. For western blotting analysis of CD44 and p53 expressions, the A549 cells were cultured... suppression. To address these questions, we examined cells of the A549 human lung adenocarcinoma line, which... express wild-type p53. In ...

  

Source: Hardy, Christopher R. - Biology Department, Millersville University

 

Collection: Environmental Sciences and Ecology

 
50 Wheat Extracts as an Efficient Cryoprotective Agent for Primary
 

Summary:  to fresh hepatocytes. Viability of A549, Caco-2, CHO-B1, HeLa, HIEC, and Jurkat cells cryopreserved... .6, and 1.3-fold increase in the viability of the Caco-2, HIEC, Jurkat, and A549 cell lines cryopreserved... cryopreserved in their respective growth media ...

  

Source: Sarhan, Fathey - Département des Sciences Biologiques, Université du Québec à Montréal

 

Collection: Biology and Medicine

 
51 ORIGINAL ARTICLE Potent antitumor efficacy of XAF1 delivered by conditionally
 

Summary:  . Materials and methods Cell lines and culture conditions The human cell lines SW620 (colorectal cancer), A549... -kD deleted ZD55-XAF1 has tumor-selective replication ability, tumor cell lines (SW620, A549... as a loading ...

  

Source: Tian, Weidong - Institute of Biochemistry and Cell Biology, Shanghai Institute of Biological Sciences

 

Collection: Biology and Medicine

 
52 Molecular Biology of the Cell Vol. 18, 44934507, November 2007
 

Summary:  ), human embryonic kidney (HEK)293, and A549 human lung carcinoma cells (ATCC CCL-185) were maintained... as described previously (Dutko and Oldstone, 1983). Virus Infection of Cells Infections of Vero, 293T, A549... -regulation of -DG protein, HEK293T and ...

  

Source: Campbell, Kevin P. - Department of Physiology and Biophysics, University of Iowa

 

Collection: Biology and Medicine

 
53 TISSUE ENGINEERING Volume 7, Number 2, 2001
 

Summary:  alveolar epithelial cell line (A549) or human bronchial epithelial cell line (16HBE14o2 ). Tissues were... of a human lung fibroblast cell line, and human lung epithelial cell lines (alveolar, A549; bronchial, 16HBE... as a ...

  

Source: George, Steven C. - Department of Biomedical Engineering, University of California, Irvine

 

Collection: Biology and Medicine

 
54 M t i Bi h iM t i Bi h i Experimental Techniques in BiophysicsExperimental Techniques in Biophysics
 

Summary:  Cantilever deflection #12;AFM imaging: contact mode AFM image of a human alveolar epithelial cell (A549) in culture... Topographic image Error image #12;AFM imaging ­ contact modeg g human alveolar epithelial cell (A549... laser spot ...

  

Source: Ritort, Felix - Departament de Fisica Fonamental, Universitat de Barcelona

 

Collection: Physics

 
55 Gold nanorod delivery of an ssRNA immune activator inhibits pandemic H1N1 influenza viral replication
 

Summary:  Nanoplexes Are Internalized by A549 Cells with Minimal Cytotoxicity. The longitudinal surface plasmon... the uptake and intracellular distri- bution of our nanoplexes (GNR-5PPP-ssRNA) in A549 cells we employed TEM... experiments in our laboratory have shown that ...

  

Source: Iowa Center for Gene Therapy

 

Collection: Biotechnology ; Biology and Medicine

 
56 Caspase-8 Association with the Focal Adhesion Complex Promotes Tumor Cell Migration and Metastasis
 

Summary:  from the American Type Culture Collection. To create FAK-deficient and control cell lines, A549 cells... ) was from Sigma. Anti-myc antibody was from Invitrogen. Cell lines. A549 lung carcinoma cells were ...

  

Source: Bogyo, Matthew - Department of Microbiology and Immunology, Stanford University

 

Collection: Biology and Medicine

 
57 Characterization of Reemerging Chikungunya Marion Sourisseau1,2
 

Summary:  an epithelial cell line (A549 alveolar cells), which was resistant to CHIKV infection. Less than 5% of the cells... Bronchial epithelial cell line þþ þþ A549 Alveolar epithelial cell line þ À Vero ...

  

Source: Ecole Polytechnique, Centre de mathématiques

 

Collection: Mathematics

 
58 The Expression of Human Microsomal Epoxide Hydrolase Is Predominantly Driven by a Genetically Polymorphic Far
 

Summary:  the specificity of the assays. Cell Culture. The hepatoma HepG2 and lung carcinoma A549 cell lines were grown... obtained from HepG2, A549, and 293A cells were loaded on a 10% SDS-polyacrylamide gel. After separation... ...

  

Source: Omiecinski, Curtis - Department of Veterinary and Biomedical Sciences, Pennsylvania State University

 

Collection: Biology and Medicine

 
59 JOURNAL OF VIROLOGY, Sept. 2010, p. 95339545 Vol. 84, No. 18 0022-538X/10/$12.00 doi:10.1128/JVI.01005-10
 

Summary:  the con- current depletion of cytoplasmic antioxidases. MATERIALS AND METHODS Cell culture. Human A549... in Hep2 cells and purified on a sucrose cushion gradient (11). A549 cells were infected with RSV... and nuclear ...

  

Source: Wood, James B. - Bermuda Biological Station for Research

 

Collection: Environmental Sciences and Ecology

 
60 JOURNAL OF VIROLOGY, Mar. 2004, p. 28632874 Vol. 78, No. 6 0022-538X/04/$08.00 0 DOI: 10.1128/JVI.78.6.28632874.2004
 

Summary:  . MATERIALS AND METHODS Cell culture. IB3 and A549 immortalized human airway cell lines were used for in vitro... a human lung carcinoma. IB3 and A549 cells were cultured as monolayers in Dulbecco's ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine


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61 THE JOURNAL OF GENE MEDICINE RESEARCH ARTICLE J Gene Med 2008; 10: 744753.
 

Summary:  NSCLC cell line A549 (ATCC CCL-185) and human transformed embryonic kidney cell line 293 (ATCC CRL-1573... . 293 and A549 cells were used in vivo, because they form tumors and allow effective (293) or adequate... viruses were propagated on 293 and ...

  

Source: Hemminki, Akseli - Department of Biosciences, University of Helsinki

 

Collection: Biology and Medicine

 
62 Evaluation of antitumor properties of novel saframycin analogs in vitro and in vivo
 

Summary:  The saframycin analog QAD (3) has been shown to inhibit the growth of A549 lung tumor cells (GI50 value 4.4 n... M (Nalm-6 B-cell leuke- mia) to a high of 10.7 nM (A549 lung tumor cells), con- firming that 3 exhibits... ) For comparison, the ...

  

Source: Myers, Andrew G.- Department of Chemistry and Chemical Biology, Harvard University

 

Collection: Chemistry

 
63 Measurement of IL-13Induced iNOS-Derived Gas Phase Nitric Oxide in Human Bronchial Epithelial Cells
 

Summary:  cell (A549) line (16). Similar results have been found in cultures of nonpulmo- nary cells (17, 18... in cultures of A549 cells (19). However, it has been shown that gene expression and iNOS activity in ...

  

Source: George, Steven C. - Department of Biomedical Engineering, University of California, Irvine

 

Collection: Biology and Medicine

 
64 Raman spectroscopy of single human tumour cells exposed to ionizing radiation in vitro This article has been downloaded from IOPscience. Please scroll down to see the full text article.
 

Summary:  human tumour cells. Prostate tumour cells (cell line DU145) are cultured in vitro and irradiated... Currently, most experimental radiobiological data are obtained from tumour cells cultured in vitro since... cultured in vitro, and observed ...

  

Source: Brolo, Alexandre G. - Department of Chemistry, University of Victoria

 

Collection: Chemistry

 
65 High-throughput screening of small molecules in miniaturized mammalian cell-based assays involving post-translational
 

Summary:  epithelial cells (Figure 2c), A549 human lung carcinoma cells (data not shown), HeLaS3 human cervical... No signal detected Antigen Chemistry & Biology #12;and trichostatin [35]. Treatment of A549 cells... histone H3. (a) 4000 ...

  

Source: Stockwell, Brent R. - Department of Biological Sciences, Columbia University

 

Collection: Biology and Medicine

 
66 JOURNAL OF VIROLOGY, 0022-538X/97/$04.00 0
 

Summary:  is essentially equivalent to that of HeLa (ATCC CCL-2) and A549 (ATCC CCL-185) cells (data not shown). As previ... between different cells (Table 1). For example, replication was observed in A549 cells at an MOI of only... of the ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
67 UNIT 1.1Basic Techniques for Mammalian Cell Tissue Culture
 

Summary:  UNIT 1.1Basic Techniques for Mammalian Cell Tissue Culture Tissue culture technology has found wide... application in the field of cell biology. Cell cultures are utilized in cytogenetic, biochemical... be grown in culture for days or weeks to ...

  

Source: George, Steven C. - Department of Biomedical Engineering, University of California, Irvine

 

Collection: Biology and Medicine

 
68 Application Note 116 Percell Biolytica AB
 

Summary:  Spher-G. In theexponentialgrowthphasethecelldoublingtime was 19 hours. During the later stages of culture a large number of cells appeared... % of the media was exchanged daily the majority of these cells were withdrawn from the culture. Discussion He... of culture an average of 70. 106 ...

  

Source: Yao, Shao Q - Department of Chemistry, National University of Singapore

 

Collection: Chemistry ; Biology and Medicine

 
69 DNA-templated assembly of droplet-derived PEG microtissues Cheri Y. Li,b
 

Summary:  streptomycin (Invitrogen). TK6 lymphoblasts (suspension culture) and A549 lung adenocarcinoma cells were... C. Cell encapsulation Prior to encapsulation, adherent cells (J2-3T3 and A549) were detached with 0... microtissues ...

  

Source: Bhatia, Sangeeta - Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology (MIT)

 

Collection: Biotechnology ; Biology and Medicine

 
70 Triple-Targeted Oncolytic Adenoviruses Featuring the Cox2 Promoter, E1A Transcomplementation, and
 

Summary:  adenocarcinoma cell line A549 (positive control for cox2 [17,26]) were obtained from the American Type Culture... -type E1A (Supplementary Fig. 2). For the positive control cell line A549 and the control cells low in cox... ...

  

Source: Hemminki, Akseli - Department of Biosciences, University of Helsinki

 

Collection: Biology and Medicine

 
71 Re.: MS: 1160590469401361 Dear Dr. Robertson,
 

Summary:  cells, but induces "only" a G1 arrest in HCT116 and A549 cancer cells [3]. In conclusion, our data show... in Cancer Cells". My colleagues and I are truly disappointed about your decision to decline our paper... that there is "no selectivity of these drugs for those cancer ...

  

Source: Wolf, Dieter A.- Burnham Institute for Medical Research

 

Collection: Biology and Medicine

 
72 Highly parallel identification of essential genes in cancer cells
 

Summary:  , and MYB were in the top 1% of essential genes in at least 1 cancer cell line (KRAS in LN229, A549, Jurkat... cell line, A549 (9th ranking gene, Dataset S1). To extend this approach to genes resident in regions... no oncogenes were previously known (26). We confirmed ...

  

Source: Sabatini, David M. - Whitehead Institute for Biomedical Research & Department of Biology, Massachusetts Institute of Technology (MIT)

 

Collection: Biology and Medicine

 
73 Respiratory Physiology & Neurobiology Special Issue on Respiratory Biomechanics
 

Summary:  in full in previous papers, e.g., A549 cell line culture was described in (Laurent et al., 2003; Planus et... epithelial A549 cell lines (AECs) and primary culture of AMs over a complete measurement cycle: e.g., ...

  

Source: Ecole Polytechnique, Centre de mathématiques

 

Collection: Mathematics

 
74 THE JOURNAL OF GENE MEDICINE RESEARCH ARTICLE J Gene Med 2010; 12: 435445.
 

Summary:  the American Type Culture Collection (ATCC, Manassas, VA, USA). 293 and A549 cell lines were cultured... was purchased from Microbix (Toronto, Ontario, Canada), and human lung adenocarcinoma cell line A549 from... Ad300wt ...

  

Source: Hemminki, Akseli - Department of Biosciences, University of Helsinki

 

Collection: Biology and Medicine

 
75 A novel oncolytic adenovirus targeting to telomerase activity in tumor cells with potent
 

Summary:  SW620, human lung cancer cell line A549, human normal embryonic lung cells WI38, human lung... tumor cell lines (Bcap37, BEL7404, Hep 3B, A549, Hela, and SW620) of various origins (breast cancer... cells (SW620, Bcap37, ...

  

Source: Tian, Weidong - Institute of Biochemistry and Cell Biology, Shanghai Institute of Biological Sciences

 

Collection: Biology and Medicine

 
76 ORIGINAL ARTICLE A three-dimensional assay for measurement of viral-induced
 

Summary:  ;respectively. The 293 and A549 cell lines were purchased from Microbix (Toronto, Canada) and the American Type... were propagated on 911 cells. Ad5-D24RGD and Ad5-D24RGD were propagated on A549 cells. The other... system superior to conventional ...

  

Source: Hemminki, Akseli - Department of Biosciences, University of Helsinki

 

Collection: Biology and Medicine

 
77 The CRAL/TRIO and GOLD domain protein TAP-1 regulates RAF-1 activation Kenneth G. Johnson, Kerry Kornfeld
 

Summary:  -target effects. To determine if TAP-1 regulates ERK signaling in additional cell types, we examined the A549 lung... form of Ras. In contrast to the MDA-MB-231 and A549 cell lines, we noted that Ras signaling in He... phosphorylation of ERK in ...

  

Source: Kopan, Raphael - Department of Molecular Biology and Pharmacology, Washington University in St. Louis

 

Collection: Biology and Medicine

 
78 THE JOURNAL OF GENE MEDICINE RESEARCH ARTICLE J Gene Med 2003; 5: 300310.
 

Summary:  carcinoma line, HepG2, and the lung carcinoma cell line, A549, were obtained from the American Type Culture... [19]. SLPI promoter activity in this cell line was 6.9% the activity of the CMV promoter. A549 cells... of ...

  

Source: Hemminki, Akseli - Department of Biosciences, University of Helsinki

 

Collection: Biology and Medicine

 
79 Gene Expression Signature of Human Cancer Cell Lines Treated with the Ras Inhibitor Salirasib (S-Farnesylthiosalicylic Acid)
 

Summary:  overexpression of tyrosine kinase recep- tors, A549 non­small cell lung carcinoma (NSCLC) cells... 87 and LAN1 cells were taken from our earlier studies (5, 6), and data on SHEP, A549, and NCIH929... of total RNA (1 Ag) from U87 ...

  

Source: Shamir, Ron - School of Computer Science, Tel Aviv University

 

Collection: Computer Technologies and Information Sciences

 
80 Microbiology (2001), 147, 23072314 Printed in Great Britain Serine/threonine protein kinases PknF and
 

Summary:  by Harlow & Lane (1988). Expression of PknF in A549 cell line. The human lung epithelial cell line A549... epithelial cell line A549 was trans- fected with the pCDNA3-pknF construct, which en- coded a ...

  

Source: Kramer, Fred - Public Health Research Center, International Center for Public Health, University of Medicine and Dentistry of New Jersey

 

Collection: Biology and Medicine


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81 The mitochondrial ARTS protein promotes apoptosis through targeting XIAP
 

Summary:  Mammalian cell culture and plasmids K562 and HL-60 cells were grown in RPMI 1640 medium. A549, COS-7 cells... carcinoma cell line (A549) in addition to NRP154 and COS-7 cells in the ...

  

Source: Steller, Hermann - Laboratory of Apoptosis and Cancer Biology, Rockefeller University

 

Collection: Biology and Medicine

 
82 [CANCER RESEARCH 64, 53905397, August 1, 2004] Effective Gene-Viral Therapy for Telomerase-Positive Cancers by Selective
 

Summary:  the American Type Culture Collection (Manassas, VA): A549 (human lung cancer cell line), PANC-1 (human pancreas... cultured in DMEM (Life Tech- nologies, Rockville, MD). A549 and PANC-1 were cultured in RPMI 1640 medium... ...

  

Source: Tian, Weidong - Institute of Biochemistry and Cell Biology, Shanghai Institute of Biological Sciences

 

Collection: Biology and Medicine

 
83 COLE DOCTORALE SCIENCES ET TECHNOLOGIES Centre de Biophysique Molculaire/Acadmie des Sciences de Pologne
 

Summary:  ............................................................................................................................... 32 a) Cell lines and cell culture conditions... ................................................................ 32 b) Primary cells and their culture ...

  

Source: Ecole Polytechnique, Centre de mathématiques

 

Collection: Mathematics

 
84 Supplemental Data Prolonged Rapamycin Treatment
 

Summary:  human rh30 human Glioblastoma u87 null human 827 null human Lung Cancer A549 human H460 human Renal... of the Raptor-mTOR but Not Rictor-mTOR Interaction A HeLa cell lysate was prepared with a CHAPS-based lysis... of the behavior of many cell lines (Figure 2), several tissues, notably the stomach and ...

  

Source: Sabatini, David M. - Whitehead Institute for Biomedical Research & Department of Biology, Massachusetts Institute of Technology (MIT)

 

Collection: Biology and Medicine

 
85 Signaling and Regulation Rictor Phosphorylation on the Thr-1135 Site Does Not Require
 

Summary:  . Mouse embryonic fibroblasts (MEF), A549, HeLa, and MDA- MB-435 cells were cultured in DMEM/F12 with 10... and culture A549, HeLa, MDA-MB-435, and MCF10A were ob- tained from the American Type Culture Collection... in the ...

  

Source: Sabatini, David M. - Whitehead Institute for Biomedical Research & Department of Biology, Massachusetts Institute of Technology (MIT)

 

Collection: Biology and Medicine

 
86 ORIGINAL ARTICLE Oncolytic adenovirus based on serotype 3
 

Summary:  ). The following cell lines were obtained from ATCC (Manassas, VA): ACHN kidney cell carcinoma, A549 lung... by second amplification in A549 cells. Oncolytic adenovirus based on serotype 3 O Hemminki et al 2 Cancer... subcutaneous injections of 2 ...

  

Source: Hemminki, Akseli - Department of Biosciences, University of Helsinki

 

Collection: Biology and Medicine

 
87 High-throughput RNAi screening in vitro: From cell lines to primary cells
 

Summary:  . Cells were transfected between passages 17 and 25. Human lung epithelial cells (A549; ATCC) were... sequence-specific gene silencing in cultured cells to study mammalian gene function. Libraries of si... of cell culture and ...

  

Source: Noriega, Fernando Gabriel - Department of Biological Sciences, Florida International University

 

Collection: Biology and Medicine

 
88 Mitochondrial Function and Nuclear Factor-KBMediated Signaling in Radiation-Induced Bystander Effects
 

Summary:  airway epithelial (A549) cells by nitric oxide. Br J Pharmacol 1997;121:1482­8. 37. Hacker H, Karin M... mitochondrial­functional cells (R+ ). However, using mixed cultures of Ro and R+ cells and targeting only one... - dependent regulation of iNOS and COX2 ...

  

Source: Nevis Laboratories, Radiological Research Accelerator Facility

 

Collection: Fission and Nuclear Technologies ; Biology and Medicine

 
89 ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Jan. 2010, p. 298304 Vol. 54, No. 1 0066-4804/10/$12.00 doi:10.1128/AAC.00973-09
 

Summary:  of a 96-well plate. For both assays, washed A549 cells were cultured with 100 l of staphylococcal... to prevent injury to cultured A549 human alveolar epithelial cells. We have previously demon- strated that ...

  

Source: Chicago, University of - Department of Microbiology

 

Collection: Biology and Medicine

 
90 Hydralazine Rescues PC12 Cells From Acrolein-Mediated Death
 

Summary:  JP. 1997. Relationships between cell density, glutathione and proliferation of A549 human lung... model in cultured PC12 cells to examine whether the adducting of hydrala- zine with acrolein... % CO2 at 378C. Culture media were changed every other day, and ...

  

Source: Shi, Riyi - Weldon School of Biomedical Engineering, Purdue University

 

Collection: Biology and Medicine ; Engineering

 
91 doi:10.1016/j.ijrobp.2004.09.018 BIOLOGY CONTRIBUTION
 

Summary:  ) and in previous reports (1, 3, 9, 10). Furthermore, in our recent study using A549 human lung cancer cells and RKO... , and appropriate numbers of cells were seeded in 25 cm2 plastic tissue culture flasks with 5 mL RPMI 1640 medium... examined. For clonogenic survival assays, mock or ...

  

Source: Gao, Jinming - Department of Oncology and Pharmacology, University of Texas Southwestern Medical Center at Dallas

 

Collection: Biology and Medicine

 
92 THE TECHNOLOGY Required performance of gels for 3D Cell Culture
 

Summary:  THE TECHNOLOGY Required performance of gels for 3D Cell Culture: (1) Ease of cell distribution; (2... ; incorporation of biologically functional molecules. BACKGROUND Commercial scaffolds for cell culture are widely... that there are some 165,000 researchers actively engaged with in vitro ...

  

Source: Mottram, Nigel - Department of Mathematics, University of Strathclyde

 

Collection: Mathematics

 
93 B 2004 Wiley Periodicals, Inc. The Opportunity of Stem Cell Bioengineering
 

Summary:  culture­based expertise in bioprocess design, technology development, and quan- titative cell fate... more than 100 years of work in tissue and ex- plant culture) and primary and transformed cell culture... review of 40 years of complex media and culture equipment ...

  

Source: Zandstra, Peter W. - Department of Chemical Engineering and Applied Chemistry, University of Toronto

 

Collection: Biotechnology

 
94 Comparison of b-Tubulin mRNA and Protein Levels in 12 Human Cancer Cell Lines
 

Summary:  (1.5­6.4%) in breast cancer cell lines, BT-549 and MCF-7; lung cancer cells, A549 and HOP 18... was not one-to- one. MATERIALS AND METHODS Cell Culture Twelve cell lines were selected on the basis... ://dtp.nci.nih.gov/docs/com- ...

  

Source: Correia, John J. - Department of Biochemistry, University of Mississippi

 

Collection: Biology and Medicine

 
95 BioMed Central Page 1 of 11
 

Summary:  have focused on the interactions of the fungus with alveolar macrophages [6,7] or with cell line A549... of Aspergil- lus fumigatus spores to A549 epithelial cells and extracellu- lar matrix proteins by a component... epithelium cell (HNEC) ...

  

Source: Ecole Polytechnique, Centre de mathématiques

 

Collection: Mathematics

 
96 Metalloprotease Inhibitors GM6001 and TAPI-0 Inhibit the Obligate Intracellular Human Pathogen
 

Summary:  to expand the resistant clones. Immunostaining of Chlamydial Inclusions--HeLa and A549 cells grown... ). Immunoblotting--HeLa or A549 cells were seeded onto 24-well plates without coverslips; infection and inhibitor... observed in human lung carcinoma ...

  

Source: Cravatt, Benjamin - Department of Cell Biology, Scripps Research Institute

 

Collection: Biology and Medicine

 
97 Pseudomonas aeruginosaExsE, a secreted regulator of type III secretion genes in Arne Rietsch, Isabelle Vallet-Gely, Simon L. Dove, and John J. Mekalanos
 

Summary:  rounding in cultured A549 lung epithelial cells. Cells were grown in RPMI supple- mented with 10% FBS 2 m... . To assess whether an exsE mutant is still capable of intoxicating cells, we infected A549 cells ...

  

Source: Mekalanos, John - Department of Microbiology and Molecular Genetics, Harvard University

 

Collection: Biology and Medicine

 
98 International Journal of Modern Physics B Vol. 23, Nos. 6 & 7 (2009) 15661571
 

Summary:  dependent manner in A549 cells. On the other hand, recent study by Hanley[7] found that ZnO nanoparticles... sterilized and ultrasonically vibrated for 10 minutes before use. L929 and Hela cells were cultured in RPMI... the living cell, apoptotic ...

  

Source: Zheng, Yufeng - Department of Advanced Materials and Nanotechnology, Peking University

 

Collection: Materials Science ; Biology and Medicine

 
99 High-sensitivity vibrational imaging with frequency modulation coherent anti-Stokes
 

Summary:  A549 human lung cancer cell cultured with deuterium-labeled oleic acids taken at =2100 cm-1 . f, FM... , of a fixed A549 human lung cancer cell cultured with deu- terated oleic acid. The forward-CARS image... species at low ...

  

Source: Heller, Eric - Departments of Chemistry & Physics, Harvard University; Xie, Xiaoliang Sunney - Department of Chemistry and Chemical Biology, Harvard University

 

Collection: Biotechnology ; Chemistry ; Materials Science ; Physics

 
100 Comparison of Rhinovirus A Infection in1 Human Primary Epithelial Cells and HeLa Cells2
 

Summary:  ; Wang et al, 2009) H292 (Bianco et al, 1998) and A549 cells (Wang et al., 2009; Peng199 et al., 2006... on rhinovirus-16 infection in427 A549 cells. Eur Respir J. 27(1), 12-9.428 Konno, S.I., Grindle, K.A., Lee, W... and maintain the29 relevant primary ...

  

Source: Palmenberg, Ann C.- Institute for Molecular Virology & Department of Biochemistry, University of Wisconsin at Madison

 

Collection: Biology and Medicine


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