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Sample search results for: aav initiator protein

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1 A Two-hybrid Screen Identifies Cathepsins B and L as Uncoating Factors for Adeno-associated Virus 2
 

Summary:  of purified cathepsin B and L proteins to bind and cleave intact AAV2 and AAV8 particles in vitro. These data... of cellular AAV infection stems from studies conducted with the AAV family prototype, AAV2. The initial step... , as ubiquitination of ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
2 JOURNAL OF VIROLOGY, Aug. 2002, p. 76517660 Vol. 76, No. 15 0022-538X/02/$04.00 0 DOI: 10.1128/JVI.76.15.76517660.2002
 

Summary:  Red.Long. In initial studies we assessed the ability of AAV2 and AAV5 to transduce retinal cells following subretinal... capsids) and AAV2 have the same trans- gene and ITRs but differ in capsid proteins. Subretinal injec... proteins of the various ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
3 Current Gene Therapy, 2003, 3, 281-304 281 1566-5232/03 $41.00+.00 2003 Bentham Science Publishers Ltd.
 

Summary:  AAV vector production is described, initial results from pre-clinical evaluation of the vectors... amount of time [Grimm and Kleinschmidt, 2000]. The enthusiasm initially associated with AAV-2 vectors... evade immune responses in animals having received an initial ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
4 JOURNAL OF VIROLOGY, Mar. 2002, p. 20432053 Vol. 76, No. 5 0022-538X/02/$04.00 0 DOI: 10.1128/JVI.76.5.20432053.2002
 

Summary:  2001 In the presence of complementing adeno-associated virus type 2 (AAV-2) Rep proteins, AAV-2 genomes... proteins may play a role as a barrier for rAAV-2 by rerouting intracellular trafficking to a nonexpressible... for 5 min at 90°C, and resolved by SDS­10% PAGE. The ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
5 Helper Virus-Free, Optically Controllable, and Two-Plasmid-Based Production of Adeno-associated
 

Summary:  , allowing detec- tion of Rep or VP proteins, or assembled capsids, of all six AAV serotypes. Finally, we... constructs for AAV protein expression, as well as their ability to support replication and encapsidation... proteins, capsid (VP) proteins from a non-type-2 ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
6 Intracellular trafficking of adeno-associated viral and JF Engelhardt1,2,3
 

Summary:  , comparative biology of these related parvoviruses has been useful to the study of AAVs. AAV was initially... attachment receptors and coreceptors As for most if not all viruses, infection with AAV is initiated... , an intracellular small GTP-binding protein.40 The ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
7 JOURNAL OF VIROLOGY, Oct. 2006, p. 98319836 Vol. 80, No. 19 0022-538X/06/$08.00 0 doi:10.1128/JVI.00878-06
 

Summary:  R). We document binding of LamR to AAV8 capsid proteins and intact virions in vitro and demonstrate its... analysis allowed us to map LamR binding to two protein subdomains predicted to be exposed on the AAV capsid... , including the initial steps of AAV8 binding and ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
8 Unique Biologic Properties of Recombinant AAV1 Transduction in Polarized Human Airway Epithelia*
 

Summary:  as a result of evolutionary diversity among capsid proteins. The identification of optimal AAV serotypes... Unique Biologic Properties of Recombinant AAV1 Transduction in Polarized Human Airway Epithelia... . In the present study, we sought to compare the biologic properties of rAAV2/1, ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
9 Recombinant AAV Viral Vectors Pseudotyped with Viral Capsids from Serotypes 1, 2, and 5 Display
 

Summary:  serotypes. The vectors contained AAV2 terminal repeats flanking a green fluorescent protein expression... Recombinant AAV Viral Vectors Pseudotyped with Viral Capsids from Serotypes 1, 2, and 5 Display... @ufl.edu. Available online 1 July 2004 Recombinant adeno-associated virus 2 (rAAV2) has been shown to ...

  

Source: Kalil, Ronald E. - Neuroscience Training Program & W.M. Keck Laboratory for Biological Imaging, University of Wisconsin at Madison; Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
10 JOURNAL OF VIROLOGY, Apr. 2005, p. 50475058 Vol. 79, No. 8 0022-538X/05/$08.00 0 doi:10.1128/JVI.79.8.50475058.2005
 

Summary:  of its major capsid protein, VP3, with that of AAV2, to which AAV4 is 58% identical and constrained... -derived growth factor recep- tors (PDGFRa and PDGFRb) have been identified as protein receptors for AAV5... capsid responsible for sialic acid binding, we initiated ...

  

Source: Baker, Timothy S. - Department of Chemistry and Biochemistry, University of California at San Diego

 

Collection: Biology and Medicine

 
11 JOURNAL OF VIROLOGY, Mar. 2004, p. 31103122 Vol. 78, No. 6 0022-538X/04/$08.00 0 DOI: 10.1128/JVI.78.6.31103122.2004
 

Summary:  .), or the anti-VP1,2,3 antibody, recognizing dissociated AAV2 capsid proteins (Amer- ican Research Products... the AAV capsid proteins had been degraded during the incubation with nuclear or cytoplasmic proteins... proteins in vitro. To rule out the possibility that ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
12 JOURNAL OF VIROLOGY, Apr. 2004, p. 33613371 Vol. 78, No. 7 0022-538X/04/$08.00 0 DOI: 10.1128/JVI.78.7.33613371.2004
 

Summary:  for replication. They are nontoxic and have not been linked to any disease. AAV capsids contain 60 protein... . A pseudo-atomic model of the AAV5 VP3 capsid protein was built by threading its amino acid sequence through... ), which compares equivalent sections. A pseudo-atomic model of the ...

  

Source: Baker, Timothy S. - Department of Chemistry and Biochemistry, University of California at San Diego

 

Collection: Biology and Medicine

 
13 original article The American Society of Gene Therapy Molecular Therapy vol. 16 no. 10, 17031709 oct. 2008 1703
 

Summary:  to yields that were only approximately fivefold lower than wild-type AAV2. Our initial goal was to determine... chosen for further analysis. Protein sequence comparison revealed similarities to AAV2 of 91... neutralization properties of each. All rAAV green fluo- rescent ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
14 Expert Review Designer Gene Delivery Vectors: Molecular Engineering and Evolution
 

Summary:  of mice in vivo (6,86). The capsid proteins of AAV10 and AAV11 showed 84 and 65% similarity, respectively... , with the AAV2 capsid protein. Systemic injection of AAV2/10 led to persistent transduction in the murine liver... mosaic vectors composed of ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
15 original article 2088 www.moleculartherapy.org vol. 17 no. 12, 20882095 dec. 2009
 

Summary:  for the structural proteins VP1-3 that assemble into the icosahedral viral capsid.15 AAV vec- tors based... -titer recombinant AAV (rAAV) cytomegalovirus (CMV) ­green fluorescent protein (GFP) vectors30 were generated, and we... ). AAV, adeno-associated virus; CMV, ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
16 GENE THERAPY Preclinical in vivo evaluation of pseudotyped adeno-associated virus vectors
 

Summary:  . These pseudotyped AAV vectors were then evaluated in immunocompetent mice for their ability to express hFIX protein... and capsid (VP) proteins, pHLP19-2, was con- structed by subcloning AAV-2 nucleotides 315 to 4534 from p... sample, corresponding to the proteins of the particular ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
17 JOURNAL OF VIROLOGY, 0022-538X/00/$04.00 0
 

Summary:  2000/Accepted 28 July 2000 Recombinant adeno-associated virus (rAAV) vectors stably transduce... hepatocytes in experimental animals. Following portal-vein administration of rAAV vectors in vivo, single... -stranded (ss) rAAV genomes become dou- ble stranded (ds), circularized, and/or concatemerized concomitant

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
18 JOURNAL OF VIROLOGY, 0022-538X/99/$04.00 0
 

Summary:  proteins were detected only in cells infected with Ad.AAV1 (Fig. 3). The signals specific for the transgene... infection with Ad.AAV1 in vitro suggested random integra- FIG. 3. Expression of viral E4 proteins and SEAP... to the terminal protein, is required to stabi- FIG. 4. Analysis of ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
19 JOURNAL OF VIROLOGY, 0022-538X/01/$04.00 0 DOI: 10.1128/JVI.75.16.76627671.2001
 

Summary:  as a gene therapy vector. Detailed sequence comparisons indicate that the AAV-5 capsid proteins are sig... Recombinant AAV production. rAAV-2 virus expressing enhanced green fluorescent protein (EGFP) was generated... analyze the interaction between sialic acid and the ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
20 JOURNAL OF VIROLOGY, June 2008, p. 58875911 Vol. 82, No. 12 0022-538X/08/$08.00 0 doi:10.1128/JVI.00254-08
 

Summary:  strategies rely on the direct physical modification of the AAV capsid protein and gene. Early examples... here are approaches to fuse targeting ligands to the N termini of AAV capsid proteins (ideally, VP2 [45... right half of the goat AAV capsid protein, which ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine


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21 Current Gene Therapy, 2001, 1, 137-147 137 1566-5232/00 $20.00+.00 2001 Bentham Science Publishers Ltd.
 

Summary:  , 2001, Vol. 1, No. 2 Sanlioglu et al. several studies have been initiated to design rAAV vectors... in order to examine the potential role of these small GTP binding proteins in AAV endocytosis... -acid targeting peptide (L14) into six different putative loops of the AAV2 capsid ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
22 JOURNAL OF VIROLOGY, Oct. 2007, p. 1130411321 Vol. 81, No. 20 0022-538X/07/$08.00 0 doi:10.1128/JVI.01225-07
 

Summary:  by cells (18). In the present study, we injected DNA repair protein-proficient or -deficient mice with rAAV... -dependent protein kinase catalytic subunit (DNA-PKcs)- and Artemis-associated endonuclease. DNA hairpin termini... of adeno-associ- ated virus (AAV) are processed by DNA repair machinery; however, ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
23 JOURNAL OF VIROLOGY, 0022-538X/99/$04.00 0
 

Summary:  shuttle vector. An rAAV shuttle vector (AV.GFP3ori) containing a green fluorescent protein (GFP) transgene... -kDa E4 (ORF6) protein. Recent observations about the helper function of adenovirus protein in rAAV... transduction have also demonstrated that adenovirus E4 ORF6 ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
24 original article 594 www.moleculartherapy.org vol. 18 no. 3, 594600 mar. 2010
 

Summary:  at 37°C with peptide libraries derived from the AAV1 or AAV5-VP1 protein at the concentration of 2µg... Society of Gene & Cell Therapy Selecting the most efficient recombinant adeno- associated virus (rAAV... promis- ing rAAV serotypes following lung coinfection. Analysis of ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
25 NATURE BIOTECHNOLOGY VOL 18 MAY 2000 http://biotech.nature.com 527 Recombinant adeno-associated virus (rAAV) vectors based on AAV
 

Summary:  AmericaInc.·http://biotech.nature.com #12;compared the mRNA and protein expression in vitro to that of pAAV-EF1-nlsLacZ, pAAV... of transgene-specific mRNAs in each transfected cell. The low levels of -gal protein expression from pAAV... protein, respectively. ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
26 Enhanced Preparation of Adeno-Associated Viral Vectors by Using High Hydrostatic Pressure to
 

Summary:  to separate AAV from the majority of the adenovirus components, but significant amounts of adenoviral protein... serotype 2 (AAV2), the first and still most broadly utilized AAV vector. For these initial experiments... be possible in an Ad5/AAV2 mixture, as our ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
27 JOURNAL OF VIROLOGY, Jan. 2005, p. 214224 Vol. 79, No. 1 0022-538X/05/$08.00 0 doi:10.1128/JVI.79.1.214224.2005
 

Summary:  by which rAAV2 vector genomes can be cross-packaged into heterologous cap- sid proteins derived from... 2 ge- nomes (delivered with AAV8 capsids) into duplex DNA. This implies that viral capsid proteins... adeno-associated virus (rAAV) vectors can mediate long-term stable transduction in ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
28 HUMAN GENE THERAPY 10:591 602 (March 1, 1999) Mary Ann Liebert, Inc.
 

Summary:  that augmentation of rAAV transduction by UV irradiation and the adenovi- ral protein E4orf6 correlates... have been demonstrated to enhance recombinant adeno-associated virus (rAAV) transduction. Such findings... are the foundation of developing new and innovative strategies to im- prove the efficiency of ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
29 JOURNAL OF VIROLOGY, Mar. 2004, p. 28632874 Vol. 78, No. 6 0022-538X/04/$08.00 0 DOI: 10.1128/JVI.78.6.28632874.2004
 

Summary:  - lutionary diversity among capsid proteins. The identification of optimal AAV serotypes for a given tissue... with proteasome inhibitors increases ubiquitination of both rAAV-2 and rAAV-2/5 capsid proteins (39). Lastly... , in vitro reconstitution assays have dem- onstrated that ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
30 [23] Adeno-Associated Virus Vectors for Short Hairpin RNA Expression
 

Summary:  ) and capsid (VP) proteins. In parallel, the wild-type AAV genome replicates to high copy numbers... ). In the cell nucleus, the AAV helper expresses Rep and VP proteins in trans (supported by adenoviral helper... the subsequent incubation of transfected cells, the Rep and VP proteins ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
31 original article 2096 www.moleculartherapy.org vol. 17 no. 12, 20962102 dec. 2009
 

Summary:  In recombinant vec- tors, genes encoding replication (rep) and capsid (cap) proteins from the wild-type AAV... strikingly similar localiza- tion patterns, only AAV2 resulted in green fluorescent protein (GFP) expression... AAV serotype Figure 4 Green fluorescent ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
32 JOURNAL OF VIROLOGY, 0022-538X/00/$04.00 0
 

Summary:  with AAV-CaPi did not require an interaction be- tween AAV capsid proteins and cell surface heparan sulfate... -CaPi and is more efficient than gene transfer with plasmid DNA-CaPi (9). Unlike plasmid DNA, AAV has viral proteins... 1999 Adeno-associated virus (AAV) is ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
33 Directed evolution of adeno-associated virus to an infectious respiratory virus
 

Summary:  5 VP1 region, which possesses only 67.7% protein sequence homology to the AAV2 VP1 region... of the AAV5 VP3 subunit were generated using Swiss Model with the coordinates of AAV2 (Protein Databank... resistance. Library Construction. For the initial plasmid library, ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
34 Capsid modifications overcome low heterogeneous expression of heparan sulfate proteoglycan that limits AAV2-mediated gene transfer and therapeutic
 

Summary:  characterized. AAV2 attachment to cells is mediated by a surface exposed loop within the AAV capsid protein... AAV vectors containing the enhanced green fluorescent protein gene (eGFP), or herpes simplex virus... the requirement for AAV second-strand DNA synthesis, ...

  

Source: Hemminki, Akseli - Department of Biosciences, University of Helsinki

 

Collection: Biology and Medicine

 
35 Loss of ATM Function Enhances Recombinant Adeno-Associated Virus Transduction and Integration through Pathways Similar to UV Irradiation
 

Summary:  binding protein (ssD-BP) has been correlated with the efficiency of rAAV transduction in certain cell... conjugates Cy3 to amine groups in viral capsid proteins through ester linkages. Purified rAAV was dialyzed... deficiency, and cancer. In this report we demonstrate that recombinant adeno-associated virus ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
36 JOURNAL OF VIROLOGY, Jan. 2006, p. 426439 Vol. 80, No. 1 0022-538X/06/$08.00 0 doi:10.1128/JVI.80.1.426439.2006
 

Summary:  IX) gene flanked by ITRs from AAV genotypes 1 through 6. Initial in vitro analyses of gfp vector DNA... the AAV-2 prototype ITR medi- ating most of these functions, i.e., the sites for Rep protein binding (rbs... recently found that among AAV-1 to -6, only AAV-5 Rep ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
37 Introduction Cystic fibrosis (CF) is the most common inherited dis-
 

Summary:  capsid proteins interferes with rAAV-2 transduction and provides new approaches to cir- cumvent... . Detection of ubiquitinated AAV capsid proteins by immunoprecipitation. To analyze the effect of the pro... by 15 days after the initial AAV infection (data not shown). ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
38 JOURNAL OF VIROLOGY, Oct. 2007, p. 1129011303 Vol. 81, No. 20 0022-538X/07/$08.00 0 doi:10.1128/JVI.00963-07
 

Summary:  ), and RAG protein-mediated transposition (45). Thus, the discovery that rAAV integrates preferentially... shown that recombinant adeno-associated virus (rAAV) vector integrates preferen- tially in genes, near... method relied on in vivo selection of rAAV integrants and could be employed for the liver

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
39 Adeno-associated viral vectors and stereotaxic
 

Summary:  to differences in capsid proteins #12;Tissue Liver AAV8, AAV9, AAVDJ Skeletal Muscle AAV1, AAV6-9 CNS AAV1, AAV2... for protein over-expression Serotype: AAV9 Protein Expressed: ...

  

Source: Chapman, Michael S. - Department of Biochemistry and Molecular Biology, Oregon Health and Science University

 

Collection: Biology and Medicine

 
40 JOURNAL OF VIROLOGY, 0022-538X/00/$04.00 0
 

Summary:  pathway was necessary to initiate intracellular movement of AAV-2 to the nucleus via both microfilaments... of viral capsid proteins (VP1, VP2, and VP3) to total protein staining. Cy3 labeling of rAAV and analysis... antibody developed against the three AAV-2 capsid ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine


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41 ORIGINAL ARTICLE Surface immobilization of hexa-histidine-tagged
 

Summary:  . In particular, we inserted a 6xHis onto a physically exposed loop of the AAV capsid protein (amino acid 587... , Berkeley, CA, USA Adeno-associated viral (AAV) vectors, which are undergoing broad exploration in clinical... the potential of AAV in a variety of therapeutic applications by reducing spread ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
42 JOURNAL OF VIROLOGY, 0022-538X/99/$04.00 0
 

Summary:  have examined whether the endocytic pathways of rAAV-2 are dependent on dynamin, a GTPase protein... approximate AAV titers of 5 1012 DNA molecules/ml and 5 108 green fluorescent protein (GFP)-expressing units... pathway. In these experiments, Cy3-AAV and FITC-transferrin were ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
43 HUMAN GENE THERAPY 18:367378 (April 2007) Mary Ann Liebert, Inc.
 

Summary:  ). Previous insertion of a flexible linker fol- lowed by a His6 tag at the end of the AAV2 VP3 capsid protein... analysis, equivalent amounts of green fluo- rescent protein (GFP) vector packaged with wild-type AAV2... . These constructs along with the other rationally de- signed His6 clones were then used to ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
44 JOURNAL OF VIROLOGY, 0022-538X/00/$04.00 0
 

Summary:  the rAAV-mediated transgene product, as deter- mined by RNA in situ hybridization and protein immunohis... California Received 3 August 1999/Accepted 5 January 2000 Recombinant adeno-associated virus vectors (rAAV... a period of 5 weeks concomitant with the conversion of the single- stranded rAAV molecules ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
45 JOURNAL OF VIROLOGY, June 2004, p. 63446359 Vol. 78, No. 12 0022-538X/04/$08.00 0 DOI: 10.1128/JVI.78.12.63446359.2004
 

Summary:  of rAAV2 encoding human glial cell line-derived neurotrophic factor (GDNF) or green fluorescent protein... - bodies (Ab) to the capsid proteins of wt AAV2, and 30 to 70% demonstrate the presence of neutralizing... demonstrated. While AAV2 capsid proteins are subject ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
46 MOLECULAR AND CELLULAR BIOLOGY, May 2003, p. 35583565 Vol. 23, No. 10 0270-7306/03/$08.00 0 DOI: 10.1128/MCB.23.10.35583565.2003
 

Summary:  supplied the Rep and Cap proteins of AAV2; pXX6, which contained the adenovirus helper functions... targeting. Whether rAAV- mediated spontaneous gene targeting is also initiated by DNA damage remains... that infection with recombinant adeno-associated virus (rAAV) vectors can mediate ...

  

Source: Baltimore, David - Division of Biology, California Institute of Technology

 

Collection: Biotechnology ; Biology and Medicine

 
47 original article 524 www.moleculartherapy.org vol. 17 no. 3, 524537 mar. 2009
 

Summary:  readministration results and extend this effect to a second transgene, green fluo- rescent protein (GFP). Unlike rAAV... of study.2,16,17 While wt-AAV and rAAV capsid proteins are subject to humoral immunity, wt-AAV can... . Groups were initially ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
48 Gene Therapy (1999) 6, 14271437 1999 Stockton Press All rights reserved 0969-7128/99 $12.00
 

Summary:  the abundance of surface AAV receptor(s) and intracellular proteins (ie, D-sequence binding protein) important... protein (ssD-BP) has been cor- related with the activation of rAAV transduction.7,8 In order to test... of protein tyrosine phosphatase signaling ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
49 ELSEVIER Virus Research 48 (1997) 41-56 Structural and functional heterogeneity of integrated
 

Summary:  #12;D. Duan et al./ Virus Research 48 (1997) 41-56 43 proteins (i.e. E4) on the recombinant AAV trans... replication of wild type AAV which occurs in the presence of rep protein and produces concatemers which... of integrated recombinant AAV genomes Dongsheng Duan b, Krishna J. Fisher ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
50 JOURNAL OF VIROLOGY, Apr. 2003, p. 47514759 Vol. 77, No. 8 0022-538X/03/$08.00 0 DOI: 10.1128/JVI.77.8.47514759.2003
 

Summary:  exonuclease. Alternatively, terminal AAV ITR could have been protected by certain cellular proteins such as D... . Berns, and T. R. Flotte. 2001. Effect of DNA- dependent protein kinase on the molecular fate of the rAAV... . Consequences of DNA-Dependent Protein Kinase Catalytic Subunit ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
51 HUMAN GENE THERAPY 16:781791 (July 2005) Mary Ann Liebert, Inc.
 

Summary:  ., 1998), although initial experiments with rAAV2 ex- pressing -galactosidase ( -Gal) in the rat brain... for terminally dif- ferentiated neurons, Neu-N (neuron-specific nuclear protein). rAAV1 and rAAV5 show higher... are un- expected because no viral proteins are ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
52 Construction of diverse adeno-associated viral libraries for directed evolution of enhanced gene
 

Summary:  virions, such that particles are composed of a mutant AAV genome surrounded by the capsid proteins encoded... large, diverse AAV2 libraries for novel gene- delivery properties21. Directed evolution of proteins... contains a mutant AAV genome that encodes the surrounding capsid ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
53 Directed evolution of adeno-associated virus yields enhanced gene delivery vectors
 

Summary:  collected from two animals before rAAV inoculations, as well as serum depleted of IgG protein through... (AAV) 2 variants with enhanced properties. High-throughput selection processes were designed to isolate... neutralization and deliver genes more efficiently than wild-type capsid in the presence of ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
54 JOURNAL OF VIROLOGY, Jan. 2005, p. 364379 Vol. 79, No. 1 0022-538X/05/$08.00 0 doi:10.1128/JVI.79.1.364379.2005
 

Summary:  systems. For example, genetic modification of AAV capsid proteins and the identification of new AAV... conformations. Despite the fact that the Rep protein-binding motifs of AAV-2 and AAV-5 ITRs are highly conserved... , it has been reported that ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
55 original article The American Society of Gene & Cell Therapy Molecular Therapy vol. 18 no. 5, 873880 may 2010 873
 

Summary:  was pseudotyped with the AAV5 capsid proteins and produced at the University of Florida Vector Core. The virus... model of MPS IIIB, neo- natal mice were treated with intracranial AAV2/5-NAGLU (AAV), syngeneic bone... marrow transplant (BMT), or both (AAV/BMT). All treatments resulted in ...

  

Source: Orrock, John - Department of Zoology, University of Wisconsin at Madison

 

Collection: Environmental Sciences and Ecology ; Biology and Medicine

 
56 JOURNAL OF VIROLOGY, 0022-538X/99/$04.00 0
 

Summary:  on the viral Rep proteins and the presence of the target sequence, i.e., AAVS1 (2, 9, 16, 18, 33). Although... January 1999/Accepted 30 March 1999 Recombinant adeno-associated virus (rAAV) vectors allow for sustained... expression of transgene products from mouse liver following a single portal vein administration. Here a ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
57 original article 2114 www.moleculartherapy.org vol.15no.12,21142123dec.2007
 

Summary:  Society of Gene Therapy Biological Differences in rAAV Transduction of Airway Epithelia in Humans... reflect the effectiveness of recombinant adeno-associated virus (rAAV)-mediated gene delivery... to assessing the biology of rAAV-mediated transduction for three serotypes, we characterized the bioelectric

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
58 Sites in the AAV5 capsid tolerant to deletions and tandem duplications Kaoru Hida a
 

Summary:  ,31], but like all the other AAV ser- otypes, it is composed of three structural proteins designated VP1, VP2... .3. Construction of the plasmid library For the initial step in the library process, the pDIM-N2-AAV5 plasmid... plasmid (pAd12) containing the VA RNA and coding for the E2 and E4 ...

  

Source: Gray, Jeffrey J. - Department of Biomolecular and Chemical Engineering, Johns Hopkins University; Ostermeier, Marc - Department of Biomolecular and Chemical Engineering, Johns Hopkins University

 

Collection: Biology and Medicine ; Biotechnology ; Chemistry

 
59 Heparin binding induces conformational changes in Adeno-associated virus Hazel C. Levy a
 

Summary:  of replicating AAV DNA and Rep protein. The presence of such highly ordered density suggests that DNA may play... Heparin sulfate a b s t r a c t Adeno-associated virus serotype 2 (AAV2) uses heparan sulfate proteoglycan... as a cell surface-attachment receptor. In this study the structures of AAV2 alone and ...

  

Source: Baker, Timothy S. - Department of Chemistry and Biochemistry, University of California at San Diego

 

Collection: Biology and Medicine

 
60 original article The American Society of Gene & Cell Therapy Molecular Therapy 1
 

Summary:  fluorescent protein (GFP) flanked by AAV2 inverted terminal repeats in the nonhuman primate basal ganglia... -specific nuclear protein (NeuN)]. The current data suggest that AAV2/5 and AAV2/1 are supe- rior to AAV2/8 for gene... a promising candidate vector for the ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine


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61 A Novel Adeno-Associated Viral Variant for Efficient and Selective Intravitreal Transduction of Rat Muller Cells
 

Summary:  with the coordinates of AAV2 (Protein Databank accession no. 1LP3) supplied as a template and images were rendered... , or intravitreal, injection of an efficient vehicle such as an adeno- associated viral vector (AAV). Although... several naturally occurring AAV variants have been isolated with a variety of ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
62 JOURNAL OF VIROLOGY, 0022-538X/98/$04.00 0
 

Summary:  Adeno-associated viral (AAV) vectors have demonstrated great utility for long-term gene expression... in muscle tissue. However, the mechanisms by which recombinant AAV (rAAV) genomes persist in muscle tissue... remain unclear. Using a recombinant shuttle vector, we have demonstrated that circularized rAAV ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
63 Recombinant Adeno-associated Viral Vectors as Therapeutic Agents to Treat Neurological Disorders
 

Summary:  Ah cDNA (rAAV-Abeta) has been used to induce autoantibodies to h-amyloid protein. Intramuscular... ]. (D) Quantification of striatal htt protein after rAAV-siHunt-1 injections. Protein levels were... @ufl.edu. Available online 18 January 2006 Recombinant adeno-associated virus ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
64 MOLECULAR THERAPY Vol. 6, No. 3, September 2002 Copyright The American Society of Gene Therapy
 

Summary:  of the GFP protein consistent with mitochondria. (G) Confocal image of the rAAV-GFP injected hemisphere... between PDH E1 and the humanized enhanced green flu- orescent protein (GFP). A control vector, rAAV-UF11... (rAAV2) Vectors Renius Owen IV, Ronald J. Mandel, Chandramohan V. Ammini, ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
65 JOURNAL OF VIROLOGY, Nov. 2002, p. 1134311349 Vol. 76, No. 22 0022-538X/02/$04.00 0 DOI: 10.1128/JVI.76.22.1134311349.2002
 

Summary:  , California 945022 Received 23 May 2002/Accepted 7 August 2002 Recombinant adeno-associated virus (rAAV... of liver transduction are not fully understood, and furthermore, the relationships between rAAV dose... with rAAV vectors, we injected mice with two different human factor IX or Escherichia coli lac

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
66 SHORT COMMUNICATION Comparative biology of rAAV transduction in ferret,
 

Summary:  SHORT COMMUNICATION Comparative biology of rAAV transduction in ferret, pig and human airway... - associated virus (rAAV)-mediated gene therapies to the lung for cystic fibrosis (CF). As new ferret and pig... the transduction biology of three rAAV serotypes (AAV1, 2 and 5) in human, ferret, pig and ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
67 Characterization of the Relationship of AAV Capsid Domain Swapping to Liver
 

Summary:  and compari- son with AAV2 is yet to be worked out. AAV capsid proteins can be divided into a series... AAV Capsid Domain Swapping 82% homology in the primary sequence between AAV2 and AAV8 capsid proteins... transduction efficiencies in the ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
68 JOURNAL OF VIROLOGY, Mar. 2005, p. 36063614 Vol. 79, No. 6 0022-538X/05/$08.00 0 doi:10.1128/JVI.79.6.36063614.2005
 

Summary:  that ITRs with sec- ondary structure are substrates for rAAV2 integration and that initial resolution... 2004 Recombinant adeno-associated virus (rAAV) vector holds promise for gene therapy. Despite a low... of rAAV integration because integration occurs preferentially in genes and accompanies chromosomal

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
69 HUMAN GENE THERAPY 18:344354 (April 2007) Mary Ann Liebert, Inc.
 

Summary:  which the vector capsid proteins are derived. Among the AAV types studied, AAV6 efficiently transduces... that with the proper AAV capsid proteins and promoter sequences, efficient trans- duction can be achieved. OVERVIEW... of enzyme product (MU) produced per minute per microgram of ...

  

Source: Miller, Dusty - Division of Human Biology, Fred Hutchinson Cancer Research Center

 

Collection: Biology and Medicine

 
70 Recombinant Adeno-associated Viral Vector-Mediated Glial Cell Line-Derived Neurotrophic Factor Gene Transfer Protects
 

Summary:  are initiated just before the rAAV-MD-GDNF administra- tion, a significant proportion of nigral DA neurons... -MD-GDNF Mediated Nigral GDNF Protein Levels Time after rAAV injection (days) n pg GDNF ipsilateral to rAAV... protein levels (pg) from 2-mm punches taken in ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
71 Adeno-Associated Virus Gene Repair Corrects a Mouse Model of Hereditary Tyrosinemia In Vivo
 

Summary:  protein-coding genes have open reading frames small enough to fit within the low coding capacity of AAV (4... to normal Fah gene and protein expression. AAV-Fah Mediates Stable Gene Repair In Vivo. The evaluation... Grompe1,5 Adeno-associated virus (AAV) vectors are ideal for performing ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
72 Unexpected off-targeting effects of anti-huntingtin ribozymes and siRNA in vivo
 

Summary:  . Levels of mHtt protein were decreased by rAAV5-HD7 in R6/1 mice. The mean levels of Hum1-immunoreactive... high-molecular weight mHtt protein for the left (uninjected) and right (rAAV5-HD7) striatum were... in the levels of mHtt protein between the untreated or ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
73 original article The American Society of Gene & Cell Therapy Molecular Therapy 1
 

Summary:  functional recovery. Additionally, we report that the levels of PTN protein expression achieved via rAAV2... that a single injec- tion of either rAAV2/1-PTN/green fluorescent protein (GFP) or rAAV2/1-GFP vector [vector... recombinant adeno-associated virus type 2/1 ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
74 Transposon-Based Mutagenesis Generates Diverse Adeno-Associated Viral Libraries with Novel Gene
 

Summary:  therapy; protein engineering. 1. Introduction Adeno-associated viral (AAV) vectors show tremendous... to generate large diverse adeno- associated viral (AAV) libraries containing a randomly located peptide... . Briefly, a selectable marker is randomly inserted throughout the AAV2 cap gene and the resulting

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
75 HUMAN GENE THERAPY 16:299306 (March 2005) Mary Ann Liebert, Inc.
 

Summary:  ,4 HIROYUKI NAKAI,1 LINDA B. COUTO,3 and MARK A. KAY1,2 ABSTRACT Recombinant adeno-associated virus (rAAV... serotype 2 (AAV-2). However, when this vector is delivered at high doses into the portal vein or hepatic... the relative level of transduction. First, we delivered an AAV vector expressing the human factor IX gene from

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
76 REVIEW ARTICLE Adeno-Associated Virus Vectors and Hematology
 

Summary:  for delivering secreted proteins such as clotting factors and cytokines. BIOLOGY OF AAV AAV is a dependent... , and leads to synthesis of the AAV proteins. In the absence of helper virus, the AAV genome can integrate... a binding site for the AAV ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
77 HUMAN GENE THERAPY 15:405413 (April 2004) Mary Ann Liebert, Inc.
 

Summary:  -associated virus (AAV) are two of the most efficient at transducing nondividing cells. We have constructed... recombinant adenovirus (AdVLacZ), adeno-associated virus serotype 2 (AAV2LacZ), and pseudotyped adeno... -associated virus serotype 5 and 8 (AAV5LacZ, AAV8LacZ) carrying the LacZ reporter, and ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
78 Convection-Enhanced Delivery of AAV Vector in Parkinsonian Monkeys; In Vivo Detection of Gene Expression and Restoration
 

Summary:  . Intrastriatal Infusion of AAV The animals were initially sedated with Ketamine (Ketaset; 10 mg/kg, im... Convection-Enhanced Delivery of AAV Vector in Parkinsonian Monkeys; In Vivo Detection of Gene... treatment strategies, which are largely based on systemically administering small molecules or proteins

  

Source: California at Davis, University of - Department of Neurology, Center for Neuroscience

 

Collection: Computer Technologies and Information Sciences ; Biology and Medicine

 
79 Characterization of Intrastriatal Recombinant Adeno-Associated Virus-Mediated Gene Transfer of Human Tyrosine Hydroxylase
 

Summary:  genes are re- moved in the case of rAAV, and hTH and hGTPCHI are cyto- plasmic enzymes, foreign proteins... ­MD­hTH rAAV­MD­ hGTPCHI groups) displayed a positive protein band at the ex- pected size for h... used high titer purified recombi- nant adeno-associated virus (rAAV) ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
80 RNA interference improves motor and neuropathological abnormalities in a Huntington's
 

Summary:  neurons provide a second target for as- sessing the effects of AAV.shHD2.1 on target protein levels... .shHD2.1, compared with uninjected or AAV.shLacZ-injected striata. Prion protein was used as a loading... , conferring a toxic gain of function on the protein huntingtin (htt). Currently, ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine


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81 rAAV2 Traffics through both the Late and the Recycling Endosomes in a Dose-Dependent Fashion
 

Summary:  Rab11 protein levels more significantly inhibited rAAV2 transduction on a per genome basis compared... of colocalization with Cy3­rAAV2 following infection at the two m.o.i. EGFP-tagged Rab fusion proteins have been... approaches were used to modulate Rab7 and Rab11 protein abundance ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
82 Robust Systemic Transduction with AAV9 Vectors in Mice: Efficient Global Cardiac Gene Transfer Superior
 

Summary:  with 86% amino acid sequence homology in the VP1 capsid protein [1]. The mecha- nism(s) by which rAAV8... Robust Systemic Transduction with AAV9 Vectors in Mice: Efficient Global Cardiac Gene Transfer... Superior to That of AAV8 Katsuya Inagaki,1 Sally Fuess,2 Theresa A. Storm,2 Gregory A. Gibson,3 Charles F

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
83 original article The American Society of Gene Therapy Molecular Therapy vol. 17 no. 2, 285293 feb. 2009 285
 

Summary:  . 2009 285 Although recombinant adeno-associated virus (rAAV) has been widely used in lung gene therapy... approaches, it remains unclear to what extent commonly used AAV serotypes transduce adult progenitors... in the lung. In this study, we evaluated the life span and proliferative capac- ity of rAAV1-, 2-, and 5

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
84 Intrastriatal rAAV-Mediated Delivery of Anti-huntingtin shRNAs Induces Partial Reversal of Disease Progression
 

Summary:  HUNT-1) resulted in reduction in the levels of mHtt mRNA (À75%) and protein (À60%). Long-term in vivo rAAV... RNA and protein levels in cultured HEK293 cells. Long-Term Striatal Expression of rAAV5-shRNAs in the R6/1 Mouse... ­37]. hrGFP protein expression was intense and paralleled the ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
85 HUMAN GENE THERAPY 12:563573 (March 20, 2001) Mary Ann Liebert, Inc.
 

Summary:  in- travenous administration of an AAV vector that expresses the coagulation protein factor X from... the hypothesis that the higher levels of protein that are XU ET AL.566 FIG. 1. (A) AAV vectors containing hAAT c... ,4 and KATHERINE PARKER PONDER1,5 ABSTRACT Although AAV vectors show ...

  

Source: Ponder, Katherine P. - Departments of Medicine & Biochemistry and Molecular Biophysics, Washington University in St. Louis

 

Collection: Biology and Medicine

 
86 Trans-splicing vectors expand the utility of adeno-associated virus for gene therapy
 

Summary:  . In vivo production of Epo protein from trans-splicing rAAV vectors in muscle. The tibialis muscles of C57... , Gainesville, FL, and approved March 23, 2000 (received for review January 5, 2000) Adeno-associated viral (AAV... on the finding that AAV genomes undergo intermolecular circular concatamerization ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
87 Zerit (Bristol-Myers) Emtriva (HIV/Gilead)
 

Summary:  -P101 (Neurologix) AAV hAADC2G ) The Yale Pharmaceutical Pipeline Preclinical Phase I Phase II Phase III... NDA/BLA Market AAV-hAADC2Genzyme) FESTINAVIR® (Oncolys/Bristol Myers Squibb) Radezolid (RX-1741) (Rib... ) Curcumin (Allertein) GGTI 2418 (Tigris) hI-conI (Iconics) AAV/dXIAP(Nuerologix) AAV/NPY ...

  

Source: Wang, Xiao-Jing - Kavli Institute for Neuroscience & Department of Neurobiology, Yale University

 

Collection: Biology and Medicine

 
88 MOLECULAR THERAPY Vol. 4, No. 4, October 2001 Copyright The American Society of Gene Therapy
 

Summary:  proteins. Our results suggested that adenoviral FIG. 1. Trans-splicing and overlapping dual rAAV vector... commonly used helper virus for AAV production. Several adenoviral proteins, including E1 and E4 proteins... transduction [17,18]. These adenoviral proteins also ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
89 LONG-TERM RESTORATION OF STRIATAL l-AROMATIC AMINO ACID DECARBOXYLASE ACTIVITY USING RECOMBINANT
 

Summary:  confirmed that all rAAV­MD­hAADC animals expressed high levels of hAADC protein (Fig. 3B). Time... that punches of striata that received rAAV­MD­hAADC virus contained levels of immunoreactive hAADC protein... encodes a protein 480 amino acids in length, the same predicted size as the ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
90 Successful transduction of liver in hemophilia by AAV-Factor IX and limitations imposed by the host
 

Summary:  for subject E, who received 2 Â 1012 vg/kg rAAV-F.IX. Arrows at upper left denote days when F.IX protein... with overlapping peptide matrices spanning the AAV-2 VP1 capsid protein and wild-type F.IX (Fig. 2). The ELISPOT... of 145 peptides spanning the sequence of the AAV-2 VP-1 ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
91 JOURNAL OF VIROLOGY, Apr. 2004, p. 41654175 Vol. 78, No. 8 0022-538X/04/$08.00 0 DOI: 10.1128/JVI.78.8.41654175.2004
 

Summary:  of these agents also affect rAAV transduction, we removed all compounds after the initial in- fection period... 28 July 2003/Accepted 2 January 2004 Recombinant adeno-associated virus (rAAV) vectors possess... of this approach using a mutant enhanced green fluorescent protein (eGFP) fluorescence recovery ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
92 Free DNA Ends Are Essential for Concatemerization of Synthetic Double-Stranded Adeno-Associated
 

Summary:  ­3], modification of capsid proteins for retargeting [4], development of rAAV vectors based on sero- types other... be addressed. Fax: (650) 498-6540. E-mail: markay@stanford.edu. Recombinant adeno-associated virus (rAAV... , we analyzed the role of inverted terminal repeat (ITR) sequences of ds circular or linear ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
93 Progress in Direct Striatal Delivery of L-Dopa via Gene Therapy for Treatment of Parkinson's Disease Using Recombinant
 

Summary:  .e., the gene therapy procedure and whether the protein functions appropriately in humans. INTRACEREBRAL rAAV... plasmids which provide the adenoviral proteins have been described, thus enabling adenovirus-free rAAV... ). Neuronal Specificity of rAAV-Mediated Gene Expression Kaplitt et al. ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
94 PEG Conjugation Moderately Protects Adeno-Associated Viral Vectors Against
 

Summary:  and in vivo (Croyle et al., 2000; O'Riordan et al., 1999). In this initial study of AAV PEGylation, we... protein function during cell binding and entry. AAV2 utilizes heparan sulfate proteoglycans (HSPG) as its... ) (Table I). This initial study of AAV PEGylation ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
95 JOURNAL OF VIROLOGY, July 2003, p. 73617366 Vol. 77, No. 13 0022-538X/03/$08.00 0 DOI: 10.1128/JVI.77.13.73617366.2003
 

Summary:  -Strand Genome Conversion of Adeno-Associated Virus Type 2 (AAV-2) and AAV-5 Is Not Rate Limiting following... Recombinant adeno-associated virus type 5 (rAAV-5) is known to efficiently transduce airway epithelia via... apical infection. In contrast, rAAV-2 has been shown to be inherently ineffective at ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
96 Anterograde delivery of brain-derived neurotrophic factor to striatum via nigral transduction of recombinant adeno-
 

Summary:  to BDNF­ rAAV transduction (Fig. 1G). There were no pronounced changes of BDNF protein levels... -derived neurotrophic factor (GDNF)­rAAV transduction supports much higher levels of GDNF protein as com- pared... viral vectors carrying brain-derived neurotrophic factor or green fluorescent ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
97 This is an Accepted Article that has been peer-reviewed and approved for publication in the Journal of Neurochemistry, but has yet to undergo copy-editing and proof correction. Please
 

Summary:  -quantitative western blot analysis. Ectopic expression of rAAV5-TH alone resulted in restoration of TH protein levels... . As an increase was observed in the TH protein levels in most groups that received rAAV5-TH, we next tested... of the virally expressed proteins, suggesting that there ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
98 Sustained Striatal Ciliary Neurotrophic Factor Expression Negatively Affects
 

Summary:  proteins dispersed within neurons or aggregated in NIIs was observed in animals receiving rAAV2-CNTF (data... - tingtin protein in vivo, CNTF was continuously expressed following transduction of the striatum... by recombinant adeno-associated viral vectors (rAAV2). Wild-type and R6/1 HD transgenic (R6/1) mice ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
99 Adenovirus Transduction is Required for the Correction of Diabetes Using Pdx-1 or
 

Summary:  mice. We delivered Pdx-1 and Ngn3 to the livers of diabetic mice using adeno-associated virus (AAV... of the transgene. We were unable to correct hyperglycemia in mice with streptozotocin- induced diabetes using AAV... liver dysmorphogenesis owing to the initiation of both endocrine and exocrine pancreas differentiation

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
100 Virus Research 104 (2004) 5159 Novel approaches to augment adeno-associated virus
 

Summary:  Available online 27 April 2004 Abstract Recombinant adeno-associated virus (rAAV) receptor binding... -limiting steps in rAAV type-2 (rAAV-2) transduction. Several strategies have been developed to enhance rAAV-2... therapy vector. To this end, the current study has investigated novel methods for augmenting ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine


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