Summary:  to solubilize and functionally stabilize CYP3A4 and other membrane proteins.33-36 Nanodiscs are self... - brane bilayer disk (Nanodisk) to functionally stabilize monomeric CYP3A4 on Ag nanoparticle ...

Source: Van Duyne, Richard P.- Department of Chemistry, Northwestern University

Collection: Chemistry

Summary:  simulation were carried out, for the WT and CYP3A4*18 proteins. As shown in Figure 3, both proteins have... were closer to SRS4 in the CYP3A4*18 than in the WT protein. Furthermore, the conformational ...

Source: Lee, Keun Woo - Department of Biochemistry, Gyeongsang National University

Collection: Biotechnology ; Biology and Medicine

Summary:  One Example of a Protein whose Stability is Controlled Partly Through Ubiquitination is _____ P53... the ________________ The _____________ of Proteins is Not Well Understood But Some Proteins ______________________While Other Last for Days Protein... ________________________ Proteasomes ...

Source: Cutler, Chris - Department of Biology, Georgia Southern University

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  secretory protein -F in a1-45 a2 a3 a4 mutants. Prepro- -F (pp -F) contains a signal sequence specific... - ture. a1-45 a2 a3 a4 and ydj1 are synthetically lethal. Several DnaJ-like proteins have been identified... of the ...

Source: Craig, Elizabeth A - Department of Biochemistry, University of Wisconsin at Madison

Collection: Biology and Medicine

Summary:  of monomeric cytochrome P450 3A4 in a nanoscale native bilayer environment. Arch Biochem Biophys 430:218­228 4... proteins, relating to their purification, stabilization, crystallization, phasing, and structure... -scale expression and purification platform to produce sufficient ...

Source: Hendrickson, Wayne A. - Department of Biochemistry and Molecular Biophysics, Columbia University; Wang, Da-Neng - Skirball Institute of Biomolecular Medicine & Department of Cell Biology, New York University

Collection: Biology and Medicine

Summary:  EIGENVALUES A1 A2 A:3 A4 11.404 10.406 9.786 9.211 where Am is the eigenvalue, m is the corresponding eigen... -local interac- tions for protein stability by structural motif engineering. J. Mol. Biol., 296:181-195, 2000. [6... ; Email:dshuang@ iim.ac.cn Abstract- This paper presents a ...

Source: Hefei Institute of Intelligent Machines - Intelligent Computing Lab

Collection: Computer Technologies and Information Sciences

Summary:  that increasing concentrations of BA have mini- mal effect on the global structure of Cyt c [Figs. 3(A), 4(A), 5(A... stability or structure,10,18,19 raising the question of whether protein stability plays any role in BA... proteins. ...

Source: Mallela, Krishna M. G. - Department of Pharmaceutical Sciences, University of Colorado at Denver

Collection: Chemistry ; Biology and Medicine

Summary:  Stability and the Evolvability of Function in a Model Protein Jesse D. Bloom,*y Claus O. Wilke... , Pasadena, California 91125 ABSTRACT Functional proteins must fold with some minimal stability... between the stability requirement and the capacity of a protein to ...

Source: Arnold, Frances H. - Division of Chemistry and Chemical Engineering, California Institute of Technology

Collection: Chemistry ; Biology and Medicine

Summary:  determination and biophysical characterization of proteins including stability and activities of SNP variants... dimensional structures of proteins of medical relevance, and place them in the public domain without... , Stockholm. The SGC works on structures of proteins from its Target List of ~3,000 ...

Source: Squire, Jeremy - Laboratory Division of Cellular and Molecular Biology, Ontario Cancer Institute, University of Toronto

Collection: Biology and Medicine

Summary:  Department of Biochemistry Module II: 9/27/10 ­ 10/29/10 99:245 Protein Stability, Folding... to protein stability, the thermodynamics and kinetics of protein folding, and molecular dynamics simulations... H on Protein Stability 10/15 F ...

Source: Iowa Center for Gene Therapy

Collection: Biotechnology ; Biology and Medicine

Summary:  for activity of the well-studied four-helix bundle protein Rop. To collect quantitative stability data... "Information in Protein Sequences: Combinatorial and Statistical Protein Design" Both the prediction and design... of protein structure, using computational and rational approaches, ...

Source: Hall, Sharon J. - School of Life Sciences, Arizona State University

Collection: Environmental Sciences and Ecology

Summary:  Evolving Protein Stability through Genetic Selection R. Luke Wiseman2,3,* and Jeffery W. Kelly1... -lactamase approach for evolving protein stability in the absence of any requirement for function. Since the discovery... is dependent on both the kinetic and thermodynamic stability of the ...

Source: Bardwell, James - Department of Molecular, Cellular and Developmental Biology, University of Michigan

Collection: Biology and Medicine ; Biotechnology

Summary:  major coat protein was derivatized with BODIPY (n-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a... ,7-dimethyl-4-bora-3a,4a- diaza-s-indacene-3-yl)methyl iodoacetamide (BODIPY FL C1-IA) or n... -sulfonaphthylamine (1,5-IAEDANS) and N-(4,4-difluoro- ...

Source: Hemminga, Marcus A. - Department of Molecular Physics, Wageningen Universiteit

Collection: Physics ; Biology and Medicine

Summary:  to the stability of proteins in stabilizing sugar-based glass. This property had been completely overlooked... methods pioneered in our group at NIST for characterizing protein stabilizing materials. · Our methods... Laboratory Stability of ...

Source: Magee, Joseph W. - Experimental Properties of Fluids Group, Physical and Chemical Properties Division, National Institute of Standards and Technology; National Institute of Standards and Technology (NIST), Materials Reliability Division

Collection: Chemistry ; Materials Science

Summary:  the extended b-sheet surface formed by the coat protein dimer (blue, green). b c Val83 V83 A(­7) A(­2) G(­3) A... Structural basis for the coevolution of a viral RNA­ protein complex Jeffrey A Chao1, Yury... Patskovsky2, Steven C Almo2 & Robert H Singer1 The cocrystal structure of the PP7 ...

Source: Singer, Robert H. - Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Yeshiva University

Collection: Biology and Medicine

Summary:  of protein fluorescence amplitudes A2, A3, A4 and A5). No other significant change or trend was observed... - escence increase (i.e. the sum of bimane fluor- escence amplitudes A2, A3, A4 and A5). Thus, A3 for G113C... measurements. Thus, for ...

Source: Mason, Jody - Department of Biological Sciences, University of Essex

Collection: Biotechnology ; Biology and Medicine

Summary:  is the basis of protein stability? What are the sequence determinants of native-like thermodynamic properties... of the cell, involves proteins. Yet our understanding of the fundamental forces that stabilize structurally... , there is no generally valid way to calculate the stability of a ...

Source: Magliery, Thomas J. - Departments of Chemistry & Biochemistry, Ohio State University; Mochrie, Simon - Department of Applied Physics, Yale University; Regan, Lynne - Department of Molecular Biophysics and Biochemistry, Yale University

Collection: Biology and Medicine ; Biotechnology ; Chemistry ; Materials Science ; Physics

Summary:  Coarse-Grained Strategy for Modeling Protein Stability in Concentrated Solutions Jason K. Cheung... the thermodynamic stability of single-domain globular proteins in concentrated aqueous solutions. Our treatment... protein concentration impacts native-state stability ...

Source: Truskett, Thomas - Department of Chemical Engineering, University of Texas at Austin

Collection: Physics ; Materials Science

Summary:  : Expression of CYP3A4, CYP2A6, and CYP2E1. Arch Biochem Biophys 345:342­354 Pugsley AP (1993) The complete... . S. Y. Lee Secretory and extracellular production of recombinant proteins using Escherichia coli... for the production of recombinant proteins. Howev- er, there are often ...

Source: Economou, Tassos - Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology, Hellas; Korea Advanced Institute of Science and Technology, Department of Chemical and Biomolecular Engineering, Metabolic & Biomolecular Engineering National Research Laboratory

Collection: Biology and Medicine ; Biotechnology ; Engineering

Summary:  of aggregates that restrict and complicate the development of proteins, antibodies and enzymes. Stabil... dramatically reduce the overall protein yield. Utilising NVoy technology, Stabil-PAC is an alternative... proprietary carbohydrate polymers, NV molecules, designed to increase the solubility and ...

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

Collection: Biotechnology ; Biology and Medicine

Summary:  mapping of the native structure, stability and folding rate in sequence space using lattice proteins has... the prototype sequence to the fringe of the neutral network, the average protein stability gradually decreases... in their stability, folding rate and evolutionary preference. The ...

Source: Levitt, Michael - Department of Structural Biology, Stanford University; Xia, Yu "Brandon" - Bioinformatics Program and Department of Chemistry, Boston University

Collection: Biology and Medicine ; Biotechnology

Summary:  0.75 -19.17 -2.0 0.78 0.98 1mi7R 1trrA 1gdtA 89 0.68 -21.58 -15.0 0.38 0.52 2audA 1tx3A 4rveB 96 0... . This result highlights the stability of trained parameters with a 40% increase in DNA binding proteins... for structure selection and stability prediction. ...

Source: Zhou, Yaoqi - School of Informatics, Indiana University

Collection: Biology and Medicine

Summary:  for secondary structure and protein stability enhancement using GFP directed evolution, circular dichroism... /topics: Course Description: While important progress has been made in the expression of proteins for structural... of problems in protein expression requires wise choices in experimental design well ...

Source: Sahinalp, S. Cenk - School of Computing Science, Simon Fraser University

Collection: Biology and Medicine ; Computer Technologies and Information Sciences

Summary:  ( 0.3 a4 0.42 0.58 0.3 k4 × 103 5.3 ( 0.1 7.3 ( 0.6 6 ( 0.1 5 ( 0.1 a Data were fit to equations... Effect of Signal Peptide on the Stability and Folding Kinetics of Maltose Binding Protein K. Beena... on the stability and folding kinetics of the ...

Source: Udgaonkar, Jayant B. - National Centre for Biological Sciences, Tata Institute of Fundamental Research

Collection: Biology and Medicine

Summary:  when a specific buffer is required for protein stability. Extraction enhanced by the addition of r... of mild detergent, protein stabilization buffer, and tris(hydroxy propyl)phosphine (THP) reducing agent... ProteinPurification andDetectionTools Second Edition Novagen® What's Inside ...

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

Collection: Biotechnology ; Biology and Medicine

Summary:  A Novel Function of Rad54 Protein STABILIZATION OF THE Rad51 NUCLEOPROTEIN FILAMENT* Received... of each protein. The binding of Rad54 pro- tein significantly stabilizes the Rad51 nucleoprotein filament... Rad54 Protein Stabilizes the Rad51 Nucleoprotein Filament14030 ...

Source: Kowalczykowski, Stephen C. - Section of Microbiology, University of California, Davis

Collection: Biology and Medicine

Summary:  , for example, the relatively rigid CYP2A6 exhibits narrow substrate specificity, whereas CYP3A4, the most... thermodynamic stabil- ity (34). More than 80% of del- eterious mutations relate to loss of protein stability... , and the con- comitant decline in the levels ...

Source: Tawfik, Dan S. - Department of Biological Chemistry, Weizmann Institute of Science

Collection: Biotechnology ; Biology and Medicine

Summary:  Analytical model for studying how environmental factors influence protein conformational stability... for probing the conformational stability of globular proteins in aqueous solution. In this approach... , the intrinsic i.e., infinite dilution thermodynamic stability and coarse structural ...

Source: Truskett, Thomas - Department of Chemical Engineering, University of Texas at Austin

Collection: Physics ; Materials Science

Summary:  given by 0iso. Most lipids are organised in bilayers and integral proteins appear to stabilize... .,. ;", E all The interaction of MI3 1 I %LL coat protein with upid bilayers 9 1 - ;% - , b - I... a spectroscopic study J.C. Sanders #12;#12;The interaction of MI3 coat protein with lipid bilayers a ...

Source: Hemminga, Marcus A. - Department of Molecular Physics, Wageningen Universiteit

Collection: Physics ; Biology and Medicine

Summary:  developed a new experimental system in which the stability of a specific protein depends on the presence... protein without affecting the stability of the resulting fusion protein, so we screened a library of FKBP... for fusion proteins that are ...

Source: Wandless, Tom - Department of Molecular Pharmacology, Stanford University

Collection: Biology and Medicine

Summary:  of the Constitutive Repressor Cleavage Activityof recA730 Protein A COMPARISON TO recA441 AND recA803 PROTEINS... and prophageinduction. We examined biochemical properties of recA730 protein in an effort to explain the constitutive... A proteininthe competitionwith single-stranded DNA binding protein (SSB ...

Source: Kowalczykowski, Stephen C. - Section of Microbiology, University of California, Davis

Collection: Biology and Medicine

Summary:  / Published: 14th September 2010 Abstract The stability of a protein's native state has important implications... - ing temperature; this property has been previously implicated as a correlate of protein stability... -tuning the stability of a protein fold. 99 ...

Source: Xia, Yu "Brandon" - Bioinformatics Program and Department of Chemistry, Boston University

Collection: Biology and Medicine

Summary:  Available online at www.sciencedirect.com Protein stability by number: high... protein stability studies into the high- throughput realm. Some methods are based on inferential read... of a vast array of proteins with different folds and functions. A huge number of ...

Source: Magliery, Thomas J. - Departments of Chemistry & Biochemistry, Ohio State University

Collection: Biology and Medicine ; Chemistry

Summary:  .08.003 Available online at www.sciencedirect.com Stability effects of mutations and protein evolvability Nobuhiko... to undermine the thermodynamic and kinetic stability of protein, is a major constraint on protein evolvability... how mutations affect protein ...

Source: Tawfik, Dan S. - Department of Biological Chemistry, Weizmann Institute of Science

Collection: Biotechnology ; Biology and Medicine

Summary:  Protein Structure and Function I. Form follows function-review of protein structure a. alpha... helices b. ß sheets c. what structure tells us about function-lacY II. All Proteins function by selective... chemical binding a. small ligand binding sites b. protein binding sites: coiled coil movie c. molecular

Source: Lycan, Deborah E. - Department of Biology, Lewis and Clark College

Collection: Biology and Medicine

Summary:  area of the union, b1 b2 b3 b4, is Atotal = (A1 + A2 + A3 + A4) - (A12 + A23 + A24 + A34) + A234. We... + A4) - (A12 + A13 + A14 + A23 + A24 + A34) + (A123 + A124 + A134 + A234) - A1234. This contains 6... ], and is thought to play important roles in protein ...

Source: Liang, Jie - Department of Bioengineering, University of Illinois at Chicago

Collection: Engineering ; Biotechnology

Summary:  to develop atomic interac- tion potentials.15,22­26 We apply the protein stability re- quirement to a simple... of the above inequality. They related the actual thermodynamic stability of a protein to the potential energy... so that the enthalpy, neces- sary for protein ...

Source: Dokholyan, Nikolay V. - Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill

Collection: Physics ; Biology and Medicine

Summary:  peptide bond #12;Polypeptides are stabilized by: 1. Covalent bonds= amide bond 2. Noncovalent, weakly... between them and stabilize regular structures=secondary structure #12;Secondary structure elements = F... structure #12;Integral membrane proteins in a lipid bilayer membrane (plasma) #12;Folded protein

Source: Economou, Tassos - Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology, Hellas

Collection: Biology and Medicine

Summary:  COMMUNICATION The Constraints Protein­Protein Interactions Place on Sequence Divergence Sarah A... on a small number of protein families have shown that residues in protein interfaces are more conserved than... . This raises the question whether protein interactions place additional con- straints on sequence ...

Source: Teichmann, Sarah - Laboratory of Molecular Biology, MRC

Collection: Biotechnology ; Biology and Medicine

Summary:  . 26S proteasome unfolds and degrades marked proteins II. Regulation of p53 activation and stability A... stability is multiply regulated Post-translational regulation is the last point of gene regulation Protein... :36031" p53 activation occurs mostly through protein ...

Source: Lycan, Deborah E. - Department of Biology, Lewis and Clark College

Collection: Biology and Medicine

Summary:  by Escherichia coli single-strand DNA binding protein. We propose that Rad52 protein acts by stabilizing the Rad... a role for Rad52 protein during presynapsis, where it stabilizes the presynaptic filament and... stimulates the rate of Rad51 protein-dependent ATP ...

Source: Kowalczykowski, Stephen C. - Section of Microbiology, University of California, Davis

Collection: Biology and Medicine

Summary:  ,7-dimethyl-4-bora-3a,4a-diaza-s-indacene- 3-dodecanoicacid (BODIPY FL C-12; Haugland, 2005; Fig. 1... M 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a- diaza-s-indacene-3-dodecanoic acid (BODIPY FL C-12... of the surrounding medium we show that protein ...

Source: Allen, John F. - School of Biological and Chemical Sciences, Queen Mary, University of London

Collection: Renewable Energy ; Biology and Medicine

Summary:  ;Cofactor covalent bonds add stability to protein structures heme #12;Post-translational modifications can... alter structure/stability #12;Protein domains! #12;Making visual sense from information overflow... BIO1502/1403 Proteins in the cell µ µ II ...

Source: Economou, Tassos - Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology, Hellas

Collection: Biology and Medicine

Summary:  at room temperature without loss of biological activity. Stabilization of proteins in the dry state can... on the stability of proteins have not yet been investigated systematically. In this thesis, the high pH buffers... . In the literature it is often claimed that optimal stability is ...

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  L5 U6 V7 U2 U10 DL9 DA8 OMe L1 V3 A4 L5 U6 V7 U2 U10 DL9 DA8 Crystal structure of the decapeptide... -H...O stabilized motif in proteins showing additional side chain-backbone hydrogen bonds www... A C-H...O Hydrogen Bond Stabilized Polypeptide Chain Reversal Motif at ...

Source: Babu, M. Madan - Laboratory of Molecular Biology, MRC

Collection: Biotechnology

Summary:  Postdoctoral Positions in Protein Engineering and Biophysics Postdoctoral positions are available... include engineering proteins and peptides for biomedical applications in regenerative medicine and cancer... and in vivo testing of therapeutically-relevant proteins that have been engineered for enhanced affinity

Source: Quake, Stephen R. - Department of Bioengineering, Stanford University

Collection: Engineering ; Biotechnology

Summary:  multiple times 3747 N. Meridian Road P.O. Box 117 Rockford, IL 61105 TR0043.0 Protein stability and storage... stability. Instead, prepare small working aliquots so that, once thawed, the protein solution will not have... concentration of 25-50% help to stabilize ...

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

Collection: Biotechnology ; Biology and Medicine

Summary:  ­475 An Integrated Probabilistic Model for Functional Prediction of Proteins MINGHUA DENG, TING CHEN, and FENGZHU SUN... ABSTRACT We develop an integrated probabilistic model to combine protein physical interactions, genetic... interactions, highly correlated gene expression networks, protein complex data, and domain structures

Source: Chen, Ting - Department of Biological Sciences, University of Southern California

Collection: Biotechnology ; Biology and Medicine ; Mathematics

Summary:  is the energy of the native structure. #12;Review Fast folding Wolynes et al. 427 Figure 3 (a) . 4 a=o.z SE, Log... ] initiated folding photochemi- tally by exploiting the fact that the stability of the protein is enhanced... Review 425 Fast-folding experiments and the topography of ...

Source: Onuchic, José - Department of Physics, University of California at San Diego

Collection: Physics ; Biology and Medicine

Summary:  February 2001 Key words: protein G, protein stabilization, protein tag Abstract Protein-fusion constructs... with solubility and stability. Here we describe the use of N-terminally fused protein G (B1 domain) as a non... the solubility and ...

Source: Zhou, Pei - Departments of Chemistry & Biochemistry, Duke University

Collection: Chemistry ; Biology and Medicine

Summary:  unstable, poly-ubiquitinated protein that can be stabilized by inhibition of proteasomal degradation. Co... that this event plays a role in the stability of this repressor protein. Blockage of the identified... -responsive genes. Most Aux/IAA proteins are short-lived and degradation of ...

Source: van den Brink, Jeroen - Leiden Institute of Physics, Universiteit Leiden

Collection: Physics

Summary:  117 a3-a4 119 a2 120 a2, bH, bE a Subscri8pts indicate 2-fold-related neighboring subunits... The Role of Scaffolding Proteins in the Assembly of the Small, Single-stranded DNA Virus fffX174... . This structure has an external scaffold made from 240 copies of protein D, 60 copies of the ...

Source: Rossmann, Michael G. - Department of Biological Sciences, Purdue University

Collection: Biology and Medicine

Summary:  increases in protein stability. The thermodynamic stability of proteins is deter- mined by the energy gap... and sequence features can reflect the intensity of stabilizing interactions for a given protein or family... of the interaction network of a ...

Source: Tawfik, Dan S. - Department of Biological Chemistry, Weizmann Institute of Science

Collection: Biotechnology ; Biology and Medicine

Summary:  -2 D-3 D-4 C-1 C-2 C-3 C-4 B-1 B-2 B-3 B-4 A-1 A-2 A-3 A-4 60 nm Control APTES Gluteraldehyde Porous... Enhanced microcontact printing of proteins on nanoporous silica surface This article has been... .1088/0957-4484/21/41/415302 Enhanced microcontact printing of proteins on nanoporous ...

Source: Zhang, Xiaojing "John" - Department of Biomedical Engineering, University of Texas at Austin

Collection: Engineering

Summary:  for extraction when a specific buffer is required for protein stability Extraction enhanced by the addition of r... utilizes a mix of mild detergents, protein stabilization buffer, and tris(hydroxypropyl)phosphine (THP... Sample Preparation Tools for Protein Research Second Edition ...

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

Collection: Biotechnology ; Biology and Medicine

Summary:  and in the test tube n Easy monitoring of protein folding and stability n Protein-protein interactions by FRET... Increasing thermal stability and folding of industrial proteins Summary: Proteins are essential parts of all... Licensable Technologies Applications: n ...

Source: Los Alamos National Laboratory, Theoretical Division, Condensed Matter and Statistical Physics Group

Collection: Materials Science

Summary:  Coarse-Grained Strategy for Modeling Protein Stability in Concentrated Solutions. III: Directional... provides new insights into how directional interactions can affect native-state protein stability... about how these factors affect protein stability comes from ...

Source: Errington, Jeffrey R. - Department of Chemical and Biological Engineering, State University of New York at Buffalo

Collection: Chemistry ; Physics

Summary:  found secondary half sites is T+1 A+3 A+4. A com- bination of these two half sites, -1T -2G -3A and T+1... A+3 A+4, is the C/A hybrid site, following pre- vious nomenclature (Izawa et al., 1993, 1994), which... ZIP proteins affects ...

Source: Guiltinan, Mark - Department of Horticulture, Pennsylvania State University

Collection: Biotechnology

Summary:  , the means by which those mutations enhance the stability and functionality of the protein were not well... of the protein surface, thereby decreasing protein aggregation. One mutation may stabilize the hydro- phobic face... , and thermal stability. We then used the ...

Source: Szostak, Jack W. - Department of Genetics, Harvard University

Collection: Environmental Sciences and Ecology ; Biology and Medicine

Summary:  Folding and Stability (im WS) oder V/S Protein Misfolding and Diseases (im SS) 30 h (2 SWS) Arbeitsaufwand... protein folding and stability. Ability to use biophysical techniques and interpret results of biophysical... and modulate the stability of a protein. The ...

Source: Baer, Christian - Institut für Mathematik, Universität Potsdam

Collection: Mathematics

Summary:  . Therefore, disulphide bridges that are necessary for the activity and stability of heterologous proteins... of proteins from their site of synthesis to their final destination in compartments inside or outside the cell... requires the crossing of membranes. The mechanisms for transport of these proteins ...

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  stability to polypeptide structures #12;Cofactor covalent bonds add stability to protein structures #12;Post... makes proteins #12;The peptide bond #12;Residue prefs in 2dary structure #12;Aminoacid rotational... flexibility Co-planar= rigidflexible #12;Polypeptides are stabilized by: 1. ...

Source: Economou, Tassos - Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology, Hellas

Collection: Biology and Medicine

Summary:  .0 0.8 Æ 0.1 1opd0 85 3a4b 70­85 (16) 1a 5.4 Æ 0.8 1.5 Æ 0.4 1.19 Æ 0.01 19 Æ 6 2.0 Æ 0.4 1.8 Æ 0.04 2... interactions as a determinant in the folding and stability of T4 lysozyme. Protein Sci 1998;7:96­104. 19... proteinsSTRUCTURE O FUNCTION O BIOINFORMATICS ...

Source: Zhou, Yaoqi - School of Informatics, Indiana University

Collection: Biology and Medicine

Summary:  they meet which will be further explained later. 2.2 Stability and Shape Analysis The motion of the protein... Mobile Robot Team Forming for Crystallization of Proteins Yuan F. Zheng1,2 and Weidong Chen2 1... The process of protein crystallization is explained using the theory of robotics, particularly path ...

Source: Zheng, Yuan F. - Department of Electrical and Computer Engineering, Ohio State University

Collection: Engineering

Summary:  Crystallographica Section D Biological Crystallography ISSN 0907-4449 Rapid and simple protein-stability screens... is thermodynamically stable for crystallization to be feasible. Thermodynamic stability is so fundamental to protein... for determining the relative stabilities of membrane ...

Source: Stowell, Michael - Department of Molecular, Cellular, and Developmental Biology, University of Colorado at Boulder

Collection: Biology and Medicine

Summary:  of disease- causing mutants--or other changes, such as ligand binding--on membrane protein stability. Pulse... of protein stability and ligand binding. Nat. Methods, 2, 207­212. 4. Schlebach, J. P., Kim, M.-S., Joh, N. H... ). Determination of protein stability in ...

Source: Clark, Patricia L. - Department of Chemistry and Biochemistry, University of Notre Dame

Collection: Biology and Medicine ; Chemistry

Summary:  Prediction of protein function using protein-protein interaction data Minghua Deng, Kui Zhang... running title: prediction of protein function Keyword: Protein-Protein Interaction, Pretein Function... -960, 2003 #12;Abstract Assigning functions to novel proteins is one of the most ...

Source: Chen, Ting - Department of Biological Sciences, University of Southern California

Collection: Biotechnology ; Biology and Medicine ; Mathematics

Summary:  stability. Proteins 2004;57:311­321. © 2004 Wiley-Liss, Inc. INTRODUCTION Inside cells, proteins fold... of cosolute with protein and the ensuing change in protein stability and conformation once cosolute... will compare thermal to cosolute denaturation. RESULTS ...

Source: Harries, Daniel - Institute of Chemistry, Hebrew University of Jerusalem; Parsegian, V. Adrian - Laboratory of Physical and Structural Biology, National Institutes of Health

Collection: Biology and Medicine ; Chemistry

Summary:  ? , a deep trough at 197 nm is observed for both TsDHN-1 and TsDHN-2 (Figs. 3a, 4a), indicating... that they stabilize proteins and mem- brane structures during environmental stress and also sequester metals... is induced and stabilized in these ...

Source: Dutcher, John - Department of Physics, University of Guelph

Collection: Materials Science ; Physics

Summary:  but overlapping sets of neurons (Figure 3(a),4). Since EXC-4 is required for the biogenesis of at least some... may be necessary for the stability of the protein. Thus, both -strands appear to be essential... Mapping Functional Domains of Chloride Intracellular Channel (CLIC) ...

Source: Hobert, Oliver - Department of Biochemistry and Molecular Biophysics, Columbia University

Collection: Biology and Medicine

Summary:  BIOPHYSICSAND COMPUTATIONALBIOLOGY Computing protein stabilities from their chain lengths Kingshuk... enough that it could be used to automatically annotate whole proteomes with protein stability information... . macromolecular confinement | protein electrostatics | ...

Source: Dill, Ken A. - Department of Pharmaceutical Chemistry, University of California at San Francisco

Collection: Biotechnology ; Biology and Medicine

Summary:  Molecular Cell Article Optimizing Protein Stability In Vivo Linda Foit,1,6,7 Gareth J. Morgan,3... mutations that stabilize proteins is chal- lenging because most substitutions are destabiliz- ing... . In addition to being of immense practical utility, the ability to evolve protein ...

Source: Bardwell, James - Department of Molecular, Cellular and Developmental Biology, University of Michigan

Collection: Biology and Medicine ; Biotechnology

Summary:  . Protein aggregation can often be avoided with proper choice of pH, salt or stabilizing additives... of the stabilizing solution. Protein Solubility Profiling NOTE: This protocol requires 2 mL of protein solution... OptiSolTM Protein Solubility Screening Kit ...

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

Collection: Biotechnology ; Biology and Medicine

Summary:  , however, we have not detected any de- stabilization of the RPA-ssDNA complex by Rad52 protein.2... Rad52 Protein Associates with Replication Protein A (RPA)-Single-stranded DNA to Accelerate Rad51... recombina- tion. This activity is stimulated by replication protein A (RPA), but only ...

Source: Kowalczykowski, Stephen C. - Section of Microbiology, University of California, Davis

Collection: Biology and Medicine

Summary:  ; nevertheless, it is possible to suggest some general guidelines for protein storage and stability. Common... .1 Protein stability and storage #12;In the USA call: 800-8-PIERCE (800-874-3723) or 815-968-0747 · Fax: 815... Stabilizing Cocktail (Product No. 89806) is a 4X solution that ...

Source: Chen, Lingling - Department of Biology, Indiana University

Collection: Biology and Medicine

Summary:  of the expressed protein, if you are having stability problems or are requesting the CSB provide all services... : For crystallization, the protein or macromolecular complex of interest should be ~98% pure and have a single... . Precipitation Point: It is advantageous to know the maximum protein or complex ...

Source: Rosenberg, Noah - Departments of Human Genetics & Biostatistics, University of Michigan

Collection: Biotechnology

Summary:  to stabilize the proteins. They are hence named osmolytes [1,2]. In another instance, mutations that impair... , it is evident that certain small molecules are effective in facilitating folding and stabilizing proteins... disulfides formed. Another advantage may be afforded on multi-domain ...

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

Collection: Biotechnology ; Biology and Medicine

Summary:  Integrated Probabilistic Model for Functional Prediction of Proteins Minghua Deng, Ting Chen... @hto.usc.edu or tingchen@hto.usc.edu ABSTRACT We develop an integrated probabilistic model to combine protein physical... interactions, genetic interactions, highly cor- related gene expression network, protein complex data

Source: Sun, Fengzhu - Sun, Fengzhu - Departments of Biological Sciences & Mathematics, University of Southern California

Collection: Mathematics ; Biotechnology

Summary:  lifetime of RecA803 protein-ssDNA complexes, demonstrating that altered protein-DNA stability... , 1985). We find that, despite our initial expectation, the stability of the RecA803 protein... kinetic stability, it associates with ssDNA more rapidly than does ...

Source: Kowalczykowski, Stephen C. - Section of Microbiology, University of California, Davis

Collection: Biology and Medicine

Summary:  volumes of dilute protein solutions, for buffer exchange, and for removing different types of salts from... protein samples. The kit is highly efficient in removing many different salts commonly used... of salts while concentrating a dilute protein solution makes the kit a convenient method for preparing

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

Collection: Biotechnology ; Biology and Medicine

Summary:  @cs.fiu.edu http://www.cs.fiu.edu/~giri/teach/BSC4934_Su10.html July 2010 #12;7/21/10 Protein Structures... ;7/21/10 Proteins" Primary structure is the sequence of amino acid residues of the protein, e.g., Flavodoxin... " Stability provided by H-bonds between CO and NH groups of residues 4 locations away. ...

Source: Narasimhan, Giri - School of Computer Science, Florida International University

Collection: Computer Technologies and Information Sciences

Summary:  Coarse-Grained Strategy for Modeling Protein Stability in Concentrated Solutions. II: Phase... stability of the native state and protein-protein interactions from basic information about protein sequence... unfolding and aggregation processes. One of the practical barriers to studying ...

Source: Errington, Jeffrey R. - Department of Chemical and Biological Engineering, State University of New York at Buffalo; Truskett, Thomas - Department of Chemical Engineering, University of Texas at Austin

Collection: Chemistry ; Materials Science ; Physics

Summary:  A7 U8 A9 R8 R7 R6 R5 R4 R3 R2 R1 ProteinRNA U1 G2 U3 A4 C5 A6 U7 A8 R8 R7 R6 R5 R4 R3 R2 R1 Fig. 1... 429:299e321. 10. Coller JM, Gray NK, Wickens MP (1998) mRNA stabilization by poly(A) binding protein... Targeted translational regulation using the PUF ...

Source: Bedwell, David M. - Department of Microbiology, University of Alabama at Birmingham; Wickens, Marv - Department of Biochemistry, University of Wisconsin at Madison

Collection: Biology and Medicine

Summary:  Prediction of protein function using protein-protein interaction data Minghua Deng, Kui Zhang... fsun@hto.usc.edu or tingchen@hto.usc.edu Abstract Assigning functions to novel proteins is one... , including ana- lyzing gene expression patterns, phylogenetic profiles, pro- tein fusions and protein-protein

Source: Sun, Fengzhu - Sun, Fengzhu - Departments of Biological Sciences & Mathematics, University of Southern California

Collection: Mathematics ; Biotechnology

Summary:  to proteases (i). Although thermal stability of a protein is a desirable property for many industrial uses... as the template protein. The inherent stability of the template molecule may have been a contributing factor... Protein Modeling and Engineering Increased Pepsin Susceptibility ...

Source: Sahinalp, S. Cenk - School of Computing Science, Simon Fraser University

Collection: Biology and Medicine ; Computer Technologies and Information Sciences

Summary:  -mail: truskett@che.utexas.edu Insights Into Crowding Effects on Protein Stability From a Coarse-Grained Model... to model the thermodynamic and kinetic aspects of protein stability in these crowded cellular or solution... to moderate protein concentrations, we find that crowding species ...

Source: Truskett, Thomas - Department of Chemical Engineering, University of Texas at Austin

Collection: Physics ; Materials Science

Summary:  -mail: truskett@che.utexas.edu Insights Into Crowding Effects on Protein Stability From a Coarse-Grained Model... to model the thermodynamic and kinetic aspects of protein stability in these crowded cellular or solution... to moderate protein concentrations, we find that crowding species ...

Source: Errington, Jeffrey R. - Department of Chemical and Biological Engineering, State University of New York at Buffalo

Collection: Chemistry ; Physics

Summary:  SYSTEMATIC ANALYSIS OF MOTIONS AND COMMUNICATION NETWORKS IN A BENCHMARK SET OF ALLOSTERIC PROTEINS... #12;ii Abstract Allosteric proteins bind an effector molecule at one site resulting in a functional... by a systematic analysis of the differences between known inactive and active structures of allosteric proteins

Source: Gray, Jeffrey J. - Department of Biomolecular and Chemical Engineering, Johns Hopkins University

Collection: Chemistry ; Biology and Medicine

Summary:  -measure protein A1­A3 4497 0.874 0.852 0.863 A1­A4 4769 0.884 0.904 0.894 A3­A4 4476 0.830 0.870 0.849 Average 0... .862 0.875 0.868 longform A1­A3 172 0.720 0.599 0.654 A1­A4 241 0.837 0.840 0.838 A3­A4 175 0.608 0.732 0... .930 ...

Source: Georgetown University Medical Center, Protein Information Resource (PIR)

Collection: Biology and Medicine

Summary:  in understanding the regulation of protein stability and folding in vitro and in the cell, the mechanisms... but not to the dissociative transition state, thereby enhancing the protein's kinetic stability. Single-molecule experiments... of proteins. Such endeavours will be important for ...

Source: Onuchic, José - Department of Physics, University of California at San Diego

Collection: Physics ; Biology and Medicine

Summary:  of RecA protein from linear ssDNA, presumably by stabilizing the RecA­ssDNA complex (27). One biochemical... Escherichia coli RecO protein anneals ssDNA complexed with its cognate ssDNA-binding protein... evidence for the functional similarity of Escherichia coli RecO protein and ...

Source: Kowalczykowski, Stephen C. - Section of Microbiology, University of California, Davis

Collection: Biology and Medicine

Summary:  Modeling Intrinsically Disordered Proteins A number of neurodegenerative disorders... such as Alzheimer's disease and Parkinson's disease involve the formation of protein aggregates. The primary... proteins (IDPs). While many proteins fold to a unique conformation that is determined by their amino acid

Source: Jackson, Daniel - Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology (MIT)

Collection: Computer Technologies and Information Sciences

Summary:  117 Summary Proteins are the media through which genetic information is expressed... or even chemical reaction catalysis. This diversity of activities is made possible because proteins come... in all sizes and "shapes" (their structures). In most cases, each protein has one unique structure (the

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  Thermal and Structural Stability of Adsorbed Proteins Sumit Sharma, B. J. Berne, and Sanat K. Kumar... are stabilized relative to the bulk. For larger coverages, proteins unfold and form b-sheets. We performed... thermo- dynamic factors then explain protein ...

Source: Berne, Bruce J. - Department of Chemistry, Columbia University

Collection: Physics ; Chemistry

Summary:  /a2 a3 2 /a4, c1 : a2, c3 : a4, c2 : a1/a2, c4 : a3/a4, hi : hi c2, and qi : qi c4, and transforming... H dependence of protein stability. Proc Natl Acad Sci USA 2003;100:4545­4550. 47. Laurents DV, Huyghes... Inferring Ideal ...

Source: Pokarowski, Piotr - Instytut Matematyki Stosowanej i Mechaniki, Uniwersytet Warszawski

Collection: Computer Technologies and Information Sciences ; Mathematics

Summary:  The continuity of protein structure space is an intrinsic property of proteins Jeffrey Skolnick1... , and approved August 3, 2009 (received for review July 9, 2009) The classical view of the space of protein... structures is that it is populated by a discrete set of protein folds. For ...

Source: Skolnick, Jeff - Center for the Study of Systems Biology, Georgia Institute of Technology

Collection: Chemistry ; Biology and Medicine

Summary:  alignments of sequences selected with SF2/ ASF, SRp40, or SRp55 are shown in Figures 3A, 4, and 5... , SRp40, or SRp55. The sequences of the B, E, and C winner series are given in Figs. 3A, 4, and 5. (N... Identification of functional exonic splicing enhancer motifs ...

Source: Zhang, Michael Q.- Watson School of Biological Sciences, Cold Spring Harbor Laboratory

Collection: Biotechnology

Summary:  of proteins and their complexes. There are several weak and strong interactions that render stability... . CONCLUSIONS Analysing the interactions that stabilize tertiary and quaternary structures and protein­protein... ,M.M and Selvaraj,S. (2004) Inter-residue interactions in protein ...

Source: Srinivasan, N. - Molecular Biophysics Unit, Indian Institute of Science, Bangalore

Collection: Biology and Medicine

Summary:  -phosphoethanolamine, triethylammonium salt) and Bodipy Fatty Acid (5-butyl-4,4-difluoro-4-bora-3a,4a- diaza... by integrating a surface sensitive mode as a tool to monitor the formation and stability of the membrane- protein... Transport, Separation, and Accumulation of ...

Source: Boston University, Center for Nanoscience and Nanobiotechnology

Collection: Materials Science

Summary:  to be a specific codon. Stabilizing the proteins expressed The hydrophobic nature of their membrane... -spanning regions makes stabilization of expressed membrane pro- teins more complex than soluble proteins. The impor... of NMR and micelles to stabilize the protein ...

Source: Stroud, Robert - Department of Biochemistry and Biophysics, University of California at San Francisco

Collection: Biology and Medicine