Summary:  myotubes, FRET efficiencies of doubly labeled 1a in puncta were similar to those of the same con- structs... or C terminus with either CFP or YFP or doubly labeled with CFP on one terminal and YFP on the other... - and Intermolecular FRET--For doubly labeled 1a, FRET ...

Source: Betz, William J. - Department of Physiology and Biophysics, University of Colorado Health Sciences Center

Collection: Biology and Medicine

Summary:  ]-glycine; TRT B (doubly labeled glycine treatment) [NH4]2SO4 U-[13 C2][15 N]-glycine; TRT C (control... NH4 13 C-glycine); solid symbols represent doubly labeled glycine (14 NH4 13 C15 N-glycine) treatment... in ...

Source: Taylor, Lee - International Arctic Research Center, University of Alaska Fairbanks; Wagner, Diane - Institute of Arctic Biology, Department of Biology and Wildlife, University of Alaska Fairbanks

Collection: Environmental Sciences and Ecology

Summary:  with a sample doubly labeled with [15 N]lysine and [methyl-13 C]methionine were carried out by measuring 13 C,1... increases the sensitivity, we prepared a sample of calmodulin doubly labeled with both [15N]lysine and [13C... ...

Source: Craik, Charles S. - Departments of Pharmaceutical Chemistry, Cellular and Molecular Pharmacology & Biochemistry and Biophysics,University of California at San Francisco

Collection: Biology and Medicine

Summary:  spectra in the rigid limit in the case of a singly labeled peptide (A) and in the case of a doubly labeled... peptide (B). The doubly labeled peptide is broadened due the dipolar interaction between the two electrons... is the spectral second moment of doubly labeled ...

Source: Hemminga, Marcus A. - Department of Molecular Physics, Wageningen Universiteit

Collection: Physics ; Biology and Medicine

Summary:  . The difference in the fluorescein decay of the singly and corresponding doubly labeled samples was utilized... analysis of the donor-acceptor distance of doubly labeled U3 MINI (main manuscript Fig. 4b... -Rh, decay data (red) with fits (black)) doubly labeled U3 ...

Source: Walter, Nils G. - Department of Chemistry, University of Michigan

Collection: Chemistry ; Biotechnology ; Biology and Medicine

Summary:  been made using doubly labeled water. Prior to extirpation of this species in the wild in 1972... leucoryx, field metabolic rate, water flux, doubly labeled water, metabolic rate, desert mammal... the doubly labeled water method, during the hot summer when plant ...

Source: Williams, Jos. B. - Department of Evolution, Ecology and Organismal Biology, Ohio State University

Collection: Environmental Sciences and Ecology

Summary:  unconditionally secure general metering schemes. As a tool to achieve this goal we introduce doubly- labelled... robust metering schemes. We introduce a new notion doubly-labelled matrices and an operation... introduce linear Secret Sharing Schemes (SSS) and Multiplicative linear SSSs. In Sect. 3 doubly- ...

Source: Nikova, Svetla Iordanova - Department of Electrical Engineering, Katholieke Universiteit Leuven

Collection: Computer Technologies and Information Sciences

Summary:  of this phenomenon, La1 was used for doubly labelling withdoubly labelling with 1515 N andN and 1313 C to investigate... --enrichment ofenrichment of urinaryurinary totaltotal--NN andand urinaryurinary ammoniaammonia total-15N OCTT 0 0,05 0,1 0... ,12 0,14 0,16 0,18 0,2 supernatant fibrinogen plasma protein ...

Source: Max-Planck-Institut für Biogeochemie, GasLab

Collection: Environmental Sciences and Ecology ; Geosciences

Summary:  microfluidic sample cell engineering · Trap doubly labeled dsDNA of varying size · Study cyclization of short... doubly labeled dsDNA of varying size · Study cyclization of short circular DNAs · Collaborations welcome... motion. A Flexible Anti-Brownian Electrokinetic (ABEL) Trap for ...

Source: Heller, Eric - Departments of Chemistry & Physics, Harvard University

Collection: Materials Science ; Physics

Summary:  (1995a) Daily energy expenditure of tufted ducks: a comparison between indirect calorimetry, doubly-labelled... and Williams TM (1995) Validation of heart-rate and doubly-labeled water as measures of metabolic-rate during... in the field: the pros and cons of the doubly labelled water and heart rate methods. ...

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  by the doubly labeled water method (DLW; Speak- man 1997); the coupling between BMR and FMR has become a central... -central region of Saudi Arabia (22 15 N, 41 50 E). Characterized as arid landscape (Meigs 1953), Ma- hazat as... labeled water method. Am J Physiol 79:39­62. ------. 1983. ...

Source: Williams, Jos. B. - Department of Evolution, Ecology and Organismal Biology, Ohio State University

Collection: Environmental Sciences and Ecology

Summary:  of the single-sex period, and DEE was measured from day 6 to day 7 using the doubly labelled water (DLW... OF ABBREVIATIONS BMR basal metabolic rate DEE daily energy expenditure DLW doubly labelled water RMR resting... ducks-a comparison between indirect calorimetry, doubly ...

Source: Simon Fraser University, Centre for Wildlife Ecology

Collection: Environmental Sciences and Ecology

Summary:  hindered C-H position, and it allows for the preparation of doubly labeled nitrile compounds for the first... to react with 15 N-labeled aqueous ammonia under the standard conditions, almost complete isotopic... ", it was envisioned that doubly labeled benzonitriles ...

Source: Chang, Sukbok - Department of Chemistry, Korea Advanced Institute of Science and Technology

Collection: Chemistry

Summary:  . Printed in the Netherlands. 127 NMR experiments for resonance assignments of 13 C, 15 N doubly-labeled... - signments in uniformly 15N, 13C-labeled flexible polypeptide chains is described. The 3D (H)N(CO-TOCSY)NH, 3... and are tailored for studies of `unfolded' ...

Source: Riek, Roland - Structural Biology Laboratory, Salk Institute for Biological Studies; Wider, Gerhard - Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule Zürich (ETHZ)

Collection: Biology and Medicine ; Biotechnology ; Chemistry

Summary:  . NMR 12, 373­383 28 Fernández, C. et al. (1998) NMR with 13C,15N-doubly- labeled DNA: the Antennapedia... of the large overall size of this protein assembly. Figure 1 shows a two-dimensional [15 N,1 H... novo protein structure determination by NMR12. Assignment of the polypeptide ...

Source: Riek, Roland - Structural Biology Laboratory, Salk Institute for Biological Studies

Collection: Biology and Medicine ; Chemistry

Summary:  reflect known differences in life history. Using the doubly labeled water method, we measured daily energy... . daily energy expenditure | energetics | life history | doubly labeled water All animals require energy... by using the doubly labeled water (DLW) method ...

Source: Pontzer, Herman - Department of Anthropology, Hunter College, City University of New York; Pontzer, Herman - Department of Anthropology, Washington University in St. Louis

Collection: Biology and Medicine

Summary:  general metering schemes. As a tool to achieve this goal we introduce doubly-labelled matrices... SSSs. In Sect. 3 doubly-labelled matrices are defined and certain properties of these matrices... (i) = 2(j) = r. 3 Operations on Doubly-Labelled Matrices Now let us consider a matrix A, which rows

Source: Nikova, Svetla Iordanova - Department of Electrical Engineering, Katholieke Universiteit Leuven

Collection: Computer Technologies and Information Sciences

Summary:  estimates of field metabolic rate (FMR) in leatherback turtles using doubly labeled water; however... Africa and then toward the Cape Verde Islands (15 N), while another went toward the Azores (39 N... , London. Booth D.T. 2002. The doubly-labeled water technique is im- practical for ...

Source: Hays, Graeme - School of Biological Sciences, University of Wales Swansea

Collection: Environmental Sciences and Ecology

Summary:  ,15N doubly labeled protomers and unlabeled protomers (Walters et al., 1997) (Figure 1B and C... ). Chemical cross-linking showed that this segment is trimeric (Park, 1995), and homonuclear TOCSY and 15N... -three interprotomer HN­HC NOEs were identified in the ...

Source: Harrison, Stephen C. - Department of Biological Chemistry and Molecular Pharmacology, Harvard University

Collection: Chemistry ; Biology and Medicine

Summary:  by doubly labelled water, during the breeding and non-breeding season are reported here. 2. FMR... from the oxidation of food stuffs. Key-words: Desert, doubly labelled water, energetics, field... study that examines the FMR and water flux, as measured by doubly ...

Source: Williams, Jos. B. - Department of Evolution, Ecology and Organismal Biology, Ohio State University

Collection: Environmental Sciences and Ecology

Summary:  by doubly labeled water over 14 days and resting metabolic rate (RMR) by indirect calorimetry. Measures... expenditure; doubly labeled water; accelerometers; phys- ical functionality ENERGY EXPENDITURE AND PHYSICAL... expenditure is by doubly labeled water (DLW) ...

Source: Batzer, Mark A. - Department of Biological Sciences, Louisiana State University

Collection: Biotechnology ; Biology and Medicine

Summary:  doubly labeled sample. Similarly, the arm, linker and DBD residue peaks were assigned on a 13 C,15 N... reached 0.8. 15 N-labeled proteins were over- expressed as described above. The purification of both DBD... deoxynucleotide extinction coefficients times their ...

Source: Fleming, Patrick - Department of Biophysics, Johns Hopkins University

Collection: Biology and Medicine ; Biotechnology

Summary:  , the method detected doubly labelled forskolin that is selectively bound only to the transport system under... are described in the remainder of this work. 3.3. DQ-¢ltered MAS NMR of doubly labelled glucose Preliminary e... of doubly labelled forskolin in frozen ...

Source: Watts, Anthony - Biomembrane Structure Unit, Department of Biochemistry, University of Oxford

Collection: Biology and Medicine

Summary:  . Doubly-labeled 15 N2O gas, purchased from CK-gas (UK), was dissolved in a degassed solution of DMSO-d6... 1 The Long-Lived Nuclear Singlet State of 15 N-Nitrous Oxide in Solution Giuseppe Pileio, Marina... lifetime (TS) of ~26 minutes for the nuclear spin singlet of ...

Source: Levitt, Malcolm H. - School of Chemistry, University of Southampton

Collection: Chemistry

Summary:  of the 20 natural amino acids plus glutathione (Gsh) (25 pmol injected). Singly- and doubly-labeled lysine... ) with the result that all amino acids are detected between 324 (singly-labeled glycine) and 643 Da (doubly-labeled... acids are marked with an asterisk (*X) and doubly-labeled lysine is marked as **K. 0.5 1.0 1.5 2.0 2.5 ...

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  -DNA to an unlabeled complement. (We denote the doubly labeled samples as TMR- 10-TR and TMR-20-TR; the singly labeled... -color emission from a doubly labeled molecule (or possibly two single-stranded DNAs in very close proximity... , to acquire spectra of doubly ...

Source: Selvin, Paul R. - Department of Physics, University of Illinois at Urbana-Champaign

Collection: Physics ; Materials Science

Summary:  glucose as the sole nitrogen and carbon sources. 15 N and 13 C doubly labeled sample was prepared... Preparation. Protein S-824 was expressed in Escherichia coli strain BL21(DE3). Uniformly 15 N-labeled sample... was pre- pared by growing cells in M9 minimal media ...

Source: Hecht, Michael H. - Departments of Chemistry & Molecular Biology, Princeton University

Collection: Chemistry ; Biotechnology

Summary:  . Using 13 C and 15 N, we observed in situ the cycling dynamics of NH4 + and glycine through various soil... pools and fine roots over 14 days. Recovery of 15 N as soil N varied with respect to N form, forest type... forest types. Microbial immobilization of 15 N was ...

Source: Wagner, Diane - Institute of Arctic Biology, Department of Biology and Wildlife, University of Alaska Fairbanks

Collection: Environmental Sciences and Ecology

Summary:  . Using 13 C and 15 N, we observed in situ the cycling dynamics of NH4 + and glycine through various soil... pools and fine roots over 14 days. Recovery of 15 N as soil N varied with respect to N form, forest type... forest types. Microbial immobilization of 15 N was ...

Source: Wagner, Diane - Institute of Arctic Biology, Department of Biology and Wildlife, University of Alaska Fairbanks

Collection: Environmental Sciences and Ecology

Summary:  and 2001 in Mahazat as-Sayd, a nature reserve in the Arabian Desert (22 15 N, 41 50 E). Skylarks (Alauda... larks in this study have been reported in Tieleman et al. (2003a). Doubly labeled water Measurements... of WIR and FMR were obtained using the doubly labeled ...

Source: Williams, Jos. B. - Department of Evolution, Ecology and Organismal Biology, Ohio State University

Collection: Environmental Sciences and Ecology

Summary:  of two-dimensional 750 MHz proton NOESY spectra of the uniformly 13 C,15 N doubly labeled protein 434... low levelspectra of a uniformly 13 C, 15 N doubly labeled protein, where the unambiguous detection... segments. For the ...

Source: Wider, Gerhard - Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule Zürich (ETHZ)

Collection: Biotechnology ; Biology and Medicine

Summary:  of two-dimensional 750 MHz proton NOESY spectra of the uniformly 13 C,15 N doubly labeled protein 434... low levelspectra of a uniformly 13 C, 15 N doubly labeled protein, where the unambiguous detection... segments. For the ...

Source: Ecole Polytechnique, Centre de mathématiques

Collection: Mathematics

Summary:  . The ESR spectrum of doubly labeled peptide I (Fig. 3 B) is clearly broadened with respect to the singly... labeled peptide II due to dipolar interaction. Consequently, the second moment of the doubly labeled... of the singly labeled peptide II (A) and doubly labeled ...

Source: Hemminga, Marcus A. - Department of Molecular Physics, Wageningen Universiteit

Collection: Physics ; Biology and Medicine

Summary:  of the small-conductance potassium channel; EF, anthrax oedema factor; HSQC, 1 H­15 N heteronuclear single... in the absence of CaM, as demonstrated by the narrow dispersion of backbone resonances in the 1 H­15 N hetero... experiments on a number of protein complex samples with various combinations of ...

Source: Ikura, Mitsuhiko - Division of Molecular and Structural Biology, University of Toronto

Collection: Biotechnology ; Biology and Medicine

Summary:  such as the doubly labeled water method. Fourth, we have constructed a phylogeny of the lark family and can take... -individual flexibility in their physiology than do species from moderate climates. An over- view of the physiological... investigates the intra- individual phenotypic flexibility of these ...

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  and stereoselective route toward doubly labeled [13 C]methyl 2- [15 N]amino - 2 - deoxy - b - D - glucopyranoside... using 13 C,15 N-labeled methyl glucoside 8 yielded the corresponding doubly labeled N... ...

Source: Wei, Alexander - Department of Chemistry, Purdue University

Collection: Materials Science ; Chemistry

Summary:  of stable isotopes (expressed as ratios 13 C/12 C, 15 N/14 N) in avian foods differ, and this `isotopic... of doubly-labelled water in incubating shorebirds (nine species, see Piersma et al. 2003; Piersma... metabolic costs. Parental energetics during incubation can be assessed by using doubly-labelled water

Source: Kleyer, Michael - Institut für Biologie und Umweltwissenschaften, Carl von Ossietzky Universität Oldenburg

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  flights and of 7 Hermit and 19 Swainson's Thrushes during stopover using doubly-labeled water (Fig. 3... AND ENERGETICS IN SMALL SONGBIRDS While the doubly-labeled water method can provide valuable insights into energy... . Furthermore, doubly-labeled water per se (without behavioral observations) cannot provide information on ...

Source: Wikelski, Martin - Department of Ecology and Evolutionary Biology, Princeton University

Collection: Environmental Sciences and Ecology

Summary:  . Since the advent of the doubly labeled water method it has been possible to measure the field met... . 1980. CO2 production in animals: Analysis of potential errors in the doubly labeled water method. Am. J... , Bloomington. Speakman, J. R. 1997. Doubly labelled ...

Source: Williams, Jos. B. - Department of Evolution, Ecology and Organismal Biology, Ohio State University

Collection: Environmental Sciences and Ecology

Summary:  of many doubly labeled SNase molecules were found to display gradual fluctuations in the donor... . (a) Emission time trace of a doubly labeled TMR Cy5 SNase molecule immobilized on glass in buffer... ) Histogram of E(t) fluctuation time constants E for 100 doubly ...

Source: Croquette, Vincent - Laboratoire de Physique Statistique, Département de Physique, École Normale Supérieure

Collection: Physics

Summary:  with solid-state nuclear magnetic resonance (NMR) methods. Chemical shift and intramolecular 13CO-15N... mol % cholesterol. Interstrand 13CO-13CO and 13CO-15N distance measurements were not consistent... , chemical shift anisotropy; dCC, 13 C-13 C dipolar coupling; dCN, 13 C- 15 ...

Source: Weliky, David - Department of Chemistry, Michigan State University

Collection: Chemistry

Summary:  probability density in selectively labeled at several positions with 13 C and 15 N in- corporated into field... field. The doublets arise from carbonyl carbon­alpha carbon pairs from a doubly labeled phenylalanine... ). The initial starting point wasand a doubly ...

Source: Prestegard, James H. - Complex Carbohydrate Research Center & Department of Chemistry, University of Georgia

Collection: Chemistry ; Biology and Medicine

Summary:  for this was obtained by NMR and EPR spectroscopy. [1H,15N]-HSQC spectra of the talin fragment indicate that vinculin... coli BL21 Star (DE3), cultured either in LB or, for preparation of 15 N/13 C-labeled samples for NMR... (Vivascience). For experiments in 100% 2 H2O, 13 C,15 ...

Source: Steinhoff, Heinz-Jürgen - Fachbereich Physik, Universität Osnabrück

Collection: Biology and Medicine ; Physics

Summary:  with different line shapes are present. Here, we present a method to decompose the spectrum of a doubly labeled... peptide that is composed of a singly labeled, noninteracting component and a doubly labeled, dipolar... of the spin label into the membrane, or, in the case of doubly ...

Source: Hemminga, Marcus A. - Department of Molecular Physics, Wageningen Universiteit

Collection: Physics ; Biology and Medicine

Summary:  ,712 613 1099 1.56 L Huli (PNG) Yamauchi et al., 2001 HRM M 15 n/a 63.6 (7.3) 3,138 (506.4) 1434 1704 1... rate monitors; TIME, time allocation; DLW, doubly labeled water. c Activity level is determined using... physical activity levels and TDEE, the most accurate of which is the doubly ...

Source: Sugiyama, Lawrence S. - Center for Ecology and Evolutionary Biology & Department of Anthropology, University of Oregon

Collection: Biology and Medicine

Summary:  labelled sample and new 15 N/13 C-edited NOESY (mixing time 100 ms) and 13 C-NOESY spectra acquired... the NH of Val64 was not observed in any experiment except the 15 N/13 C-edited nuclear Overhauser effect... ; however, at least 12 sharp peaks could be observed in the ...

Source: Williamson, Mike P. - Department of Molecular Biology and Biotechnology, University of Sheffield

Collection: Biology and Medicine

Summary:  using a new 15 N2 method, and found a strong negative relationship between the ratio of N2:C2H4 and N2... fixationrates (Anderson et al. in final prep) (see Figure). He is currently focusing on how 15 N2 fixation rates... of these processes (McFarland, in final prep). These studies have involved the use of ...

Source: Ruess, Roger W. - Institute of Arctic Biology, Department of Biology and Wildlife, University of Alaska Fairbanks

Collection: Environmental Sciences and Ecology

Summary:  conditions: a study with doubly labeled water. Ornis Scandinavica, 24, 161-164. Carmi-Winkler, N., Degen, A... -energy budget, food intake and doubly labeled water methods. Ardea, 76, 64-81. Mathieu-Costello, O., Agey, P. J

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  between the amide 15N and the C. #12;11 Threonines are characterized by strong C-C cross peaks (doubly... . Printed in the Netherlands. 1 Resonance assignment of 13 C/15 N labeled solid proteins by two- and three... - specific assignment of 13C and ...

Source: Hong, Mei - Department of Chemistry, Iowa State University

Collection: Chemistry

Summary:  costs in the studies mentioned before were measured with the doubly labelled water (DLW) method. DLW... might have been able to compensate for the workload. We know only little about the flexibility

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  incubation with 5 mM NH4Cl, doubly-labelled glutamine was also detectable (ratio of mono-/doubly labelled... glutamine, corrected for natural abundance, is 3:2; fig. 3b). MSO treatment caused the appearance of doubly-labelled... glutamate with a ratio of mono-/doubly-labelled glutamate ...

Source: Diekmann, Martin - Institut für Ökologie und Evolutionsbiologie, Universität Bremen

Collection: Environmental Sciences and Ecology

Summary:  (Centre for Isotope Research) CW Continuous Wave DLW Doubly Labelled Water DSP Digital Signal Processing

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  and neighboring amide 15 N nuclei) depend on the kinetics of slow exchange processes, as well... as on the populations of the conformations and chemical shift differences of 13 C and 15 N nuclei. The relaxation rates... ) internal dynamics as reflected in relaxation rates (R1, R2, and Overhauser effects) of ...

Source: Ecole Normale Supérieure & Universite Pierre et Marie Curie, Nuclear Magnetic Resonance Group

Collection: Chemistry

Summary:  of the DPPC acyl chains. The 20-13 C/15 N REDOR ex- periments using doubly labeled anandamide with 15 N in its... different nuclei, such as 13 C­31 P, 13 C­15 N, and 13 C­2 H (21, 22). This technique uniquely allows... C, ...

Source: Yang, De-Ping - Department of Physics, College of the Holy Cross

Collection: Biology and Medicine ; Materials Science

Summary:  Measurements. Steady-state FRET measurements of HDV ribozyme doubly labeled with fluorescein... complexes doubly labeled with fluorescein and tetramethylrhodamine. The annealed ribozyme complexes (150 µ... L; 1 µM doubly labeled ribozyme strand B, 3 µM strand A, ...

Source: Walter, Nils G. - Department of Chemistry, University of Michigan

Collection: Chemistry ; Biotechnology ; Biology and Medicine

Summary:  Aust. J. Zool., 1990, 38, 469-77 Doubly Labelled Water Validation in the Marsupial Petauroides... by means of potassium hydroxide absorption, and by the low-level, doubly labelled water method, which... methods yielded significantly different values, but doubly ...

Source: Foley, Bill - School of Botany and Zoology, Australian National University

Collection: Environmental Sciences and Ecology ; Biology and Medicine

Summary:  estimated by the doubly labelled water method Published in 2003 in Journal of Experimental Biology, 206... .e. crushing). Energy expenditures, estimated with the doubly labelled water (DLW) method calibrated for use... and subcutaneously with a precisely known amount of doubly-labelled ...

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  or sparse NOEs [1, 2, 3]. However, these algorithms either require several sets of RDCs and an 15 N,13 C... doubly-labeled sample, or rely heavily on molecular dynamics or statistics from the Protein Databank (PDB

Source: Lilien, Ryan - Department of Computer Science, University of Toronto; Mettu, Ramgopal - Department of Electrical and Computer Engineering, University of Massachusetts at Amherst

Collection: Computer Technologies and Information Sciences ; Engineering

Summary:  by ss-FRET. The doubly labeled ribozyme displays a strong signal for the acceptor fluorophore... of these mea- surements. The hairpin ribozyme is doubly labeled with a donor (fluorescein) and an acceptor... ­ substrate complex is measured. Then, the donor flu- orescence decay in the donor­acceptor ...

Source: Walter, Nils G. - Department of Chemistry, University of Michigan

Collection: Chemistry ; Biotechnology ; Biology and Medicine

Summary:  angle around the central Ca­Cb bond [12]. 2. Materials and methods 13 C0 and 15 N doubly labeled glycine... experiment for extracting / angles on 13 C0 , 15 N doubly labeled N-t-Boc­glycine. The /H-angle dependence... . The ...

Source: Hong, Mei - Department of Chemistry, Iowa State University

Collection: Chemistry

Summary:  ., Géloën, A., Pachiaudi, C., Gharib, C., Normand, S., Validation of the Doubly Labeled Water Method in Rats... .L., Midwood, A.J., Estimation of Energy Expenditure in Free-living Red Deer (Vervus Elaphus) with the Doubly-Labelled... Group), The Doubly-Labelled Water Method for Measuring Energy Expenditure, ...

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  . Separately, stopped- flow FRET was carried out using an optimized doubly labeled DNA substrate in which... , a substantial FRET of 40% is observed in the DNA duplex doubly labeled with Fl and RhX fluorophores covalently... ). For this reason, the additional energy transfer from Fl to RhX upon the addition of ...

Source: Reich, Norbert O. - Department of Chemistry and Biochemistry, University of California at Santa Barbara

Collection: Chemistry ; Biology and Medicine

Summary:  of experiments with doubly labeled precursors. Samples of [1(R,S)-3H]-, [l(R)-)H]-, and [1(S)-3H... study terpenoid and polyketide metaboli~m,~precursors doubly labeled with carbon-13 have been used... .' used a variation of the doubly labeled acetate technique in ...

Source: Boxer, Steven G. - Department of Chemistry, Stanford University

Collection: Renewable Energy ; Chemistry ; Biology and Medicine

Summary:  Scandinavica 20, 156-158. Speakman, J. R. 1997. Doubly labelled water: Theory and practice. London: Chapman... -66. T Tatner, P. & Bryant, D. M. 1989. The doubly labelled water technique for measuring energy expenditure. In... -living female savannah spar- rows during incubation - A study using ...

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  to the energy expenditure (ef, in W) estimated via doubly labelled water measurements conducted under comparable... , food balance, mass loss, direct respirometry (with a respiratory mask), doubly labelled water (DLW... and the doubly labelled water methods, that ...

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  . Freeman, San Francisco Speakman JR (1997) Doubly labelled water. Theory and practice. Chapman and Hall... :457-470 T Tatner P, Bryant DM (1986) Flight costs of a small passerine measured using doubly labelled water... ) Validation of the doubly labeled water method in ...

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  curves of E. coli under conditions that enable rapid switching from unlabeled to [15N]ammonia (filter... from unlabeled to [15N]ammonia. (c) Pathways of ammonia assimilation in E. coli; (2) indicates... , glutamate dehydrogenase; GS, glutamine synthetase. (d) Kinetics of formation of ...

Source: Rabinowitz, Joshua D. - Lewis-Sigler Institute for Integrative Genomics & Department of Molecular Biology, Princeton University

Collection: Biology and Medicine ; Biotechnology

Summary:  expenditure · DEE · Doubly labeled water · Food availability · Black- legged kittiwake · Pacific herring... in 1997 and 1998 at 2 colonies in PWS. The North Icy Bay colony (60° 15' N, 148° 20' W, ca. 1500 pairs... consisting of each age class. Energy expenditure. We used the ...

Source: Jodice, Patrick - Department of Forestry and Natural Resources, Clemson University

Collection: Environmental Sciences and Ecology

Summary:  . 3b)27 as well as 15 N- and 13 C- edited nuclear Overhauser enhancement spectro- scopy (NOESY... -peaks of 1 H­15 N HSQC spectrum of the FH domain of FOXO3a are assigned, and the assignments of 93 (of 97... structural insights into this FH­CR3 interaction. The 15 ...

Source: Ikura, Mitsuhiko - Division of Molecular and Structural Biology, University of Toronto

Collection: Biotechnology ; Biology and Medicine

Summary:  Homonuclear decoupled 13 C chemical shift anisotropy in 13 C doubly labeled peptides by selective... ° pulse lengths were 3.5 ls for 1 H and 5 ls for 13 C and 15 N. The 1 H decoupling field was 75 kHz during... acquisition and increased to 95 kHz during the CSA evolution. A 15 ...

Source: Hong, Mei - Department of Chemistry, Iowa State University

Collection: Chemistry

Summary:  of fluorescein-tetra- methylrhodamine doubly labeled constructs, showing either green (donor)- or red (acceptor... To further examine the role of joiner J1/2 in the global structure, we doubly-labeled the common PEX-1B... /2 that share the same doubly labeled 39- segment. The donor and ...

Source: Walter, Nils G. - Department of Chemistry, University of Michigan

Collection: Chemistry ; Biotechnology ; Biology and Medicine

Summary:  dimensions or plumage. Doubly labelled water experiments Measurements of DEE (kJ/day) were made in a variety... of weather conditions, using the Doubly Labelled Water (DLW) method (Lifson and McClintock 1966; Speakman... for every doubly labelled water experiment and ...

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  in Bacillus subtilis. Three-dimensional 1H, 15N, and 13C experiments have been used to obtain full side chain... atoms. Analyses of backbone 15N relaxation measurements demonstrate relative rigidity in most regions... for protein-protein interactions. EXPERIMENTAL PROCEDURES Sample Preparation. Uniformly ...

Source: McIntosh, Lawrence P. - Department of Biochemistry and Molecular Biology, University of British Columbia

Collection: Biotechnology ; Biology and Medicine

Summary:  to identify sequential residues, and 1 H-15 N HSQC cross-peaks to unambiguously distinguish G from U imino... of exchangeable NOESY and 1 H-15 N HSQC spectra, with se- quential connectivities drawn and resonance assignments... . Dashed lines connect imino-amino NOEs with the diagonal. Bottom panel depicts ...

Source: Walter, Nils G. - Department of Chemistry, University of Michigan

Collection: Chemistry ; Biotechnology ; Biology and Medicine

Summary:  experiment is attractive also for use with proteins that are available in 13 C/15 N doubly labeled form... . Printed in The Netherlands. J-Bio NMR 478 Arginine side chain assignments in uniformly 15 N... (NE)HGHH, is presented for the assignment of arginine side chain ...

Source: Wider, Gerhard - Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule Zürich (ETHZ)

Collection: Biotechnology ; Biology and Medicine

Summary:  ] unications of the ACM, v.15 n.12, pp.1053- [12] ulation Interoperability [13] , MA, [14] ulations... Language Support for Identifying Flexible Points in Coercible Simulations Joseph C. Carnahan Paul F... @virginia.edu, reynolds@virginia.edu, brogan@virginia.edu Keywords: COERCE, coercible simulations, ...

Source: Brogan, David - Susquehanna International Group

Collection: Computer Technologies and Information Sciences

Summary:  as doubly-labeled Cy3­DNA­FL were gel- purified and were each determined to be .95% free from other... for the singly-labeled DNA­FL (orange), as well as for the doubly- labeled Cy3­DNA­FL (red). FL emission... in the doubly-labeled sample is substantially quenched relative to its emission in DNA­FL, ...

Source: Levin, Judith G. - Laboratory of Molecular Genetics, National Institutes of Health

Collection: Biology and Medicine

Summary:  -energy budget, food intake and doubly labelled water methods. Ardea 76: 64-81. Masman, D., C. Dijkstra, S. Daan... .R. 1997. Doubly labelled water: Theory and practice. Chapman & Hall. Speakman, J.R. & J. McQueenie. 1996... flexibility in garden warblers Sylvia borin during ...

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  . The decay of fluorescein emission in the doubly- labeled complex was analyzed by a model of distance... using time-resolved fluorescence reso- nance energy transfer (tr-FRET). a, Schematic of the basic doubly... - labeled ribozyme­substrate com- plex. The two-strand hairpin ribo- zyme (Rz) binds the ...

Source: Walter, Nils G. - Department of Chemistry, University of Michigan

Collection: Chemistry ; Biotechnology ; Biology and Medicine

Summary:  , and the structural transparency offered by 6Li-15N doubly labeled LiHMDS in conjunction with 6Li, 15N, and 13C NMR... , Cornell UniVersity, Ithaca, New York 14853-1301 ReceiVed May 30, 1996X Abstract: 6Li, 15N, and 13C NMR... ...

Source: Collum, David B. - Department of Chemistry and Chemical Biology, Cornell University

Collection: Chemistry

Summary:  et al., 1996), or from free-ranging birds using the time­energy budget (TEB) and the doubly labelled... by Kooyman et al. (1992a), who measured values between 2.4 and 4.8·W·kg­1 using doubly labelled water... dive limit DLT diving lactate threshold DLW doubly ...

Source: Fahlman, Andreas - Department of Zoology, University of British Columbia

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  individuals using the doubly labelled water technique, which averaged 609.9 kJ day-1 . We integrated... , energy-rich copepods. Keywords Doubly labelled water Á Dovekie Á Foraging behaviour Á Greenland Á Time... procedure We used the two-point doubly labelled ...

Source: Gregoire, Arnaud - Centre d’Ecologie Fonctionnelle et Evolutive

Collection: Biology and Medicine

Summary:  individuals after journeys of up to 600 km and determining their energy expenditure by using doubly labelled... ) thrushes, we injected 38 birds (each weighing about 30 g; ref. 10) with 0.1 ml doubly labelled water... .4, n 26, P<0.001). We calibrated measurements using doubly ...

Source: Wikelski, Martin - Department of Ecology and Evolutionary Biology, Princeton University

Collection: Environmental Sciences and Ecology

Summary:  = Daily Energy Expenditure (kJ d-1) DL = Dry lean mass (g) DLW = Doubly labeled Water ERS = Event

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  .R.Th., Neubert, R.E.M., Meijer, H.A.J., McLean, M., Visser, G.H., Validation of the Doubly Labeled Water Method

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  -called `doubly-labelled water method' have revolutionized our understanding of energy requirements of wild... . New York: Cambridge University Press. Speakman JR (1997) Doubly Labelled Water: Theory and Practice... and specialists, although more comparative studies are needed to deter- mine if dietary ...

Source: McWilliams, Scott R. - Department of Natural Resources Science, University of Rhode Island

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  protein was separated from unlabelled and doubly labelled protein by ion exchange chromatography (MonoQ HR... by electrospray ionization mass spectroscopy, were labelled with Alexa Fluor 594 maleimide. Doubly labelled... (Amersham Pharmacia) and labelled with Alexa Fluor 594 succinimidyl ester. ...

Source: Lipman, Everett A. - Department of Physics, University of California at Santa Barbara; Schuler, Ben - Biochemisches Institut, Universität Zürich

Collection: Biology and Medicine ; Chemistry ; Physics

Summary:  indicate that KAPP may be a negative regulator of the BRI1 signaling transduction pathway. 15N-Labeled KI... of FHA Domain of KAPP Biochemistry, Vol. 46, No. 10, 2007 2685 #12;15 N KI-FHA NMR Titrations... with Phosphopeptides. 15 N- Labeled KI-FHA was prepared with ...

Source: Skolnick, Jeff - Center for the Study of Systems Biology, Georgia Institute of Technology

Collection: Chemistry ; Biology and Medicine

Summary:  as energy expenditure excluding that which is deposited into tissue, was measured using the doubly labelled... distributed along the fitted curve through the body mass range (Runs test: N1 = 15, N2 = 14, U = 12, P > 0... : analysis of potential errors in the doubly ...

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  is typically measured using time allocation, doubly-labeled water (DLW), or heart rate (HR) monitors, but all... are available for estimating physical activity levels and TDEE, the most accurate of which is the doubly labeled

Source: Sugiyama, Lawrence S. - Center for Ecology and Evolutionary Biology & Department of Anthropology, University of Oregon

Collection: Biology and Medicine

Summary:  with natural flight. We measured energy expenditure during flight was with the doubly labelled water (DLW... in body mass. Heart rate measurements have a high time resolution in contrast to the doubly labelled water... of flight was measured with the doubly labelled ...

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  not shown). The data for the doubly labeled mutant L37R1/A107R1 (Fig. 4 e) are also significantly... behavior is observed for the doubly labeled mutant A107R1/S223R1 (Fig. 4 f). Again the data are sig... a high flexibility of the studied loop regions. An increase of the distance between ...

Source: Steinhoff, Heinz-Jürgen - Fachbereich Physik, Universität Osnabrück

Collection: Biology and Medicine ; Physics

Summary:  that is 2.6-fold larger than doubly labeled TMR-K560 (red spectrum). Adding a 5-fold molar excess... the fluorescence of doubly labeled TMR-K560 to that in the absence of microtubules Author contributions: S... by demonstrating 1-step photobleaching for singly labeled TMR- K560 and 2-step photobleaching for ...

Source: Selvin, Paul R. - Department of Physics, University of Illinois at Urbana-Champaign

Collection: Physics ; Materials Science

Summary:  that is 2.6-fold larger than doubly labeled TMR-K560 (red spectrum). Adding a 5-fold molar excess... the fluorescence of doubly labeled TMR-K560 to that in the absence of microtubules Author contributions: S... by demonstrating 1-step photobleaching for singly labeled TMR- K560 and 2-step photobleaching for ...

Source: Yildiz, Ahmet - Departments of Molecular and Cell Biology & Physics, University of California at Berkeley

Collection: Biology and Medicine ; Physics

Summary:  ´andez, T. Szyperski, A. Ono, H. Iwai, S. Tate, M. Kainosho, and K. W¨uthrich, NMR with 13C,15N-doubly-labeled... acceptor 15 N. As a reference, the cross-correlated relaxation be- tween 1 H chemical shift anisotropy... and dipole­dipole coupling of 1 H and its hydrogen bond donor 15 ...

Source: Riek, Roland - Structural Biology Laboratory, Salk Institute for Biological Studies

Collection: Biology and Medicine ; Chemistry

Summary:  costs estimated by the doubly labelled water method. Journal of Experimental Biology, 206, 3361... vary flexibly in size (Piersma & Lindström 1997; Piersma & Drent 2003), and it has recently been shown... on intake rates, organ flexibility is therefore likely to lead to differences in foraging ...

Source: Groningen, Rijksuniversiteit - Centre for Ecological and Evolutionary Studies, Department of Marine Benthic Ecology and Evolution,

Collection: Biology and Medicine ; Environmental Sciences and Ecology

Summary:  ) and 12kHz from resonance (bottom). ionic), doubly labelled with 13C and 15N at the peptide bond [ 11J

Source: Pines, Alexander - Department of Chemistry, University of California at Berkeley; Suter, Dieter - Fachbereich Physik, Universität Dortmund

Collection: Chemistry ; Physics

Summary:  at position 76 was studied by trFRET analysis of ribozyme complexes doubly labeled with fluorescein... and tetramethylrhodamine as previously described (18). Annealed ribozyme complexes (70 µL; 1 µM doubly labeled ribozyme... the acceptor in place. The effect of the acceptor on the decay of fluorescein ...

Source: Walter, Nils G. - Department of Chemistry, University of Michigan

Collection: Chemistry ; Biotechnology ; Biology and Medicine

Summary:  , this was achieved with a 1:4 labeled/unlabeled mixture that leads to a negligible amount of doubly labeled dimers... on the dipolar broadening because of the recep- tor-transducer interaction, the spectra of the doubly labeled... obtained from the simulations of low temperature spectra of singly labeled ...

Source: Steinhoff, Heinz-Jürgen - Fachbereich Physik, Universität Osnabrück

Collection: Biology and Medicine ; Physics

Summary:  and the doubly labeled Leu, and correction was made for the spillover from the [1-13 C]Leu (M + 1) into [15 N, 1... by using [15 N, 1-13 C]Leu kinetics measured during amino acid (AA) infusion and a hyperinsulinemic... by continuous infusion (8 h) of ...

Source: Bequette, Brian J. - Department of Animal and Avian Sciences, University of Maryland at College Park

Collection: Biology and Medicine