| Sample search results for: abc excision nuclease |
| 1 | Communications Conformation-Specific Recognition of Carcinogen-DNA Adduct in | ||
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Summary: . Nucleotide Excision Assays. The 5-terminally labeled DNA substrates (2 nM) were incised by UvrABC (UvrA, 15 n... adducts by UvrABC nuclease is adduct- and sequence-specific: comparison of the rates of UvrABC nuclease... nucleotide excision ... |
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Source: Cho, Bongsup P. - Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island |
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Collection: Biology and Medicine |
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| 2 | Diversity visualization by endonuclease: A rapid assay to monitor diverse nucleotide libraries | ||
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Summary: Theam Soon Lim a,b,c , Tatjana Schütze a,b , Hans Lehrach a , Jörn Glökler a , Zoltán Konthur a... 2010 Available online 23 December 2010 Keywords: S1 nuclease Diversity Assay Nucleotide library Phage... nuclease. The assay involves only a lim- ited number of steps and can be performed in less than 4 h |
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Source: Konthur, Zoltán - Max-Planck-Institut für molekulare Genetik |
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Collection: Biology and Medicine |
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| 3 | Identification of BFN1, a Bifunctional Nuclease Induced during Leaf and Stem Senescence in Arabidopsis1 | ||
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Summary: Identification of BFN1, a Bifunctional Nuclease Induced during Leaf and Stem Senescence... 1312 Nuclease I enzymes are responsible for the degradation of RNA and single-stranded DNA during several plant... - dopsis, and demonstrate that it is a senescence-associated nuclease I gene. BFN1 nuclease shows high |
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Source: Green, Pamela - Delaware Biotechnology Institute & Department of Plant and Soil Sciences, University of Delaware |
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Collection: Biotechnology ; Biology and Medicine |
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| 4 | Copyright 2009 by the Genetics Society of America DOI: 10.1534/genetics.109.108472 | ||
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Summary: of a lig4 mutant background for piggyBac or Minos excision screens. Zinc-finger nucleases (ZFNs) have... of the Bloom Syndrome DNA Helicase Extends the Utility of Imprecise Transposon Excision for Making Null... for imprecise excision screens to delete genes of interest. However, these screens are highly ... |
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Source: McVey, Mitch - Department of Biology, Tufts University |
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Collection: Biology and Medicine |
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| 5 | Plant Science, 81 (1992)93-102 93 ElsevierScientificPublishers Ireland Ltd. | ||
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Summary: Bgeneproductsduring riceplastiddevelopmentis light-regulatedprimarily at translational or post-translational level. Sl-nuclease mapping... . A heterologous in vitro transcription assaywith spinach transcriptionally active high salt extract, followedby Sl-nuclease... was characterized by nuclease S1 mapping and primer extension. ... |
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Source: Chung, Kuang-Ren - Citrus Research and Education Center, University of Florida |
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Collection: Biology and Medicine |
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| 6 | Supplemental Data Passenger-Strand Cleavage Facilitates Assembly | ||
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Summary: in water. Sample recovery was about 50%. Diastereomer identity was established by nuclease P1 treatment... . Samples were treated with 2 units nuclease P1 (US Biological) for 60 min at 37°C, dephosphorylated... -30% Buffer B over 35 min. The characteristic nuclease P1-resistant Rp CU dinucleotide eluted from the column |
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Source: Bartel, David - Whitehead Institute for Biomedical Research & Department of Biology, Massachusetts Institute of Technology (MIT) |
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Collection: Biotechnology ; Biology and Medicine |
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| 7 | Review Article Strategies for DNA Interstrand Crosslink Repair | ||
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Summary: , it appears that this organism has evolved an excision nuclease-based mecha- nism of repairing ICLs... ; homologous recombination; nucleotide excision repair; translesion synthesis INTRODUCTION DNA interstrand... pathways, including nucleo- tide excision repair (NER) and homologous recombination (HR). ... |
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Source: McVey, Mitch - Department of Biology, Tufts University |
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Collection: Biology and Medicine |
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| 8 | Summary and general discussion Even though DNA has the very important task of storing all genetic information its | ||
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Summary: . Examples of these are base excision repair (BER), UV damage endonuclease (UVDE) repair and nucleotide... excision repair (NER). Base excision repair requires the presence of a relatively large number of proteins... B and UvrC. The molecular mechanism of the UvrABC system has been extensively studied and is ... |
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Source: van den Brink, Jeroen - Leiden Institute of Physics, Universiteit Leiden |
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Collection: Physics |
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| 9 | Mutation Research 485 (2001) 2336 Nucleotide excision repair "a legacy of creativity" | ||
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Summary: of XPA/RPA to which the excision nucleases XPG and XPF/ERCC1 bind and carry out the 3 and 5 cleavages... systems, e.g. XPG-nth, an association between the NER 3 nuclease with the glycosylase for excision... Mutation Research 485 (2001) 2336 Nucleotide excision repair "a legacy of ... |
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Source: Karplus, Kevin - Department of Biomolecular Engineering, University of California at Santa Cruz |
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Collection: Biotechnology ; Chemistry |
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| 10 | THESIS APPROVAL The abstract and thesis of Katherine Ona for the Master of Science in Biology were | ||
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Summary: . Biochimie 64:595-598. 41. Sancar, A., and W. D. Rupp. 1983. A novel repair enzyme: UVRABC excision nuclease... and recovery following #12;2 treatment with nitrofurazone requires recA, nucleotide excision repair, the rec... excision repair pathways are important for cell survival in the presence of ... |
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Source: Courcelle, Justin - Department of Biology, Portland State University |
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Collection: Biology and Medicine |
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| 11 | Encyclopaedia of Life Science--Editor Stylesheet Copyright Macmillan Reference Ltd 20 November 1998 Page 1 | ||
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Summary: -repair nuclease subunit A; part of the UvrABC endonuclease that initiates excision repair uvrB 17.5 Helicase... and ATPase; excision-repair nuclease subunit B; part of the UvrABC endonuclease that initiates excision... repair uvrC 43.0 ... |
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Source: Kowalczykowski, Stephen C. - Section of Microbiology, University of California, Davis |
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Collection: Biology and Medicine |
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| 12 | RecA Protein Piero R Bianco,University of California, Davis, California, USA | ||
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Summary: with UmuD and Umu(D')2 uvrA 91.9 Excision-repair nuclease subunit A; part of the UvrABC endonuclease... that initiates excision repair uvrB 17.5 Helicase and ATPase; excision-repair nuclease subunit B; part of the UvrABC... endonu- clease that ... |
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Source: Kowalczykowski, Stephen C. - Section of Microbiology, University of California, Davis |
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Collection: Biology and Medicine |
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| 13 | Abasic Site Repair in Higher Eukaryotes Phyllis R. Strauss and Noreen E. O'Regan | ||
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Summary: Base-excision repair (BER) refers to a repair pathway that generates and repairs aba- sic sites... polymerase (pol ) fills the gap with the correct nucleotide (reaction 3) and then excises the dRP from... of PCNA causes accumulation of pre-excision reaction intermediates following strand displacement, which |
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Source: Strauss, Phyllis R. - Department of Biology, Northeastern University |
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Collection: Biology and Medicine |
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| 14 | RADIATION RESEARCH 168, 499506 (2007) 0033-7587/07 $15.00 | ||
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Summary: , A novel repair enzyme: UVRABC excision nuclease of Escherichia coli cuts a DNA strand on both sides... complete degradation of the genomic DNA occurs. Although the RecBCD helicase/nuclease complex is known... -dependent helicase and nuclease that is capable of un- winding and degrading duplex DNA from a ... |
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Source: Courcelle, Justin - Department of Biology, Portland State University |
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Collection: Biology and Medicine |
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| 15 | The obesity-associated Fto gene is a transcriptional coactivator Qiong Wu a,1 | ||
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Summary: ,1 , Maria Szkudlarek-Mikho a , Ivana de la Serna b , Khew-Voon Chin a,b,c, a Department of Medicine... /EBPs) from unmethylated as well as methylation-inhibited gene promoters. Fto also exhibits nuclease activity... and base excision re- pair via the excision of methylated base by DNA glycosylases [33], or ... |
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Source: Toledo, University of - Center for Diabetes and Endocrine Research (CeDER) |
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Collection: Biology and Medicine |
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| 16 | Purification and Characterization of the DNA Polymerase R Associated Exonuclease: The RTH1 Gene Product | ||
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Summary: of RTH1 nuclease revealed that most likely the enzyme can excise an A or T much faster than a G or C. One... is also known as yeast RAD2 homolog 1 or RTH1 nuclease. The native exonuclease also had strong flap... endonuclease activity similar to that observed with RTH1 nuclease and homologous yeast (RAD2) and ... |
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Source: McQuade, D. Tyler - Department of Chemistry and Biochemistry, Florida State University |
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Collection: Chemistry |
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| 17 | Ctp1 and Exonuclease 1, alternative nucleases regulated by the MRN complex, are required | ||
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Summary: 10 nuclease, required for nucleotide excision- repair, is required for efficient recombination... Ctp1 and Exonuclease 1, alternative nucleases regulated by the MRN complex, are required... homologous DNA, which can produce genetic recombinants. To determine the role of nucleases in DSB end |
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Source: Smith, Gerry - Basic Sciences Division, Fred Hutchinson Cancer Research Center |
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Collection: Biology and Medicine |
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| 18 | Homingendonucleasestructureandfunction Barry L. Stoddard | ||
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Summary: , as well as inteins (intervening sequences that are spliced and excised post... a common ancestral nuclease. While crystal structures of several representatives of the LAGLIDADG... in proper splicing and excision of the RNA intron, forming a stable RNP in the process. The spliced intron |
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Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle |
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Collection: Biology and Medicine |
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| 19 | Selective Excision of the Centromere Chromatin Complex from Saccharomycescerevisiae | ||
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Summary: Selective Excision of the Centromere Chromatin Complex from Saccharomycescerevisiae Margaret... a CEN fragment that can be selec- tively excised from the chromatin DNA with restric- tion endonucleases... of centromere homology is resistant to nuclease digestion. Restriction enzyme linkers encod- ing the Bam HI |
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Source: Bloom, Kerry S.- Department of Biology, University of North Carolina at Chapel Hill |
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Collection: Biology and Medicine |
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| 20 | Holliday junctions in the eukaryotic nucleus: resolution in sight? | ||
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Summary: ,9]. Integrase and nuclease superfamilies of HJ resolvases: the RuvABC model and archaeal Hjc Homologous... action of RuvABC is well-supported by genetic evidence, and mutations in all three genes result... activi- ties suggest that the RuvABC mechanism might be conserved in eukaryotes [15 |
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Source: Heyer, Wolf-Dietrich - Sections of Microbiology & Molecular and Cellular Biology, University of California, Davis |
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Collection: Biology and Medicine |
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| 21 | THE ROLE OF RECOMBINATION PROTEINS IN MAINTAINING AND PROCESSING ARRESTED REPLICATION FORKS DURING THE | ||
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Summary: by the RecJ/RecQ, nuclease/helicase.......................................................34 Figure 1... I, nucleotide excision repair and branch migration by RecG or RuvAB are not required to generate... by the RecJ/RecQ, nuclease/helicase............................................................40 1.6 Model |
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Source: Courcelle, Justin - Department of Biology, Portland State University |
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Collection: Biology and Medicine |
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| 22 | Molecular Cell, Vol. 15, 3141, July 2, 2004, Copyright 2004 by Cell Press A Defined Human System that Supports | ||
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Summary: that Supports Bidirectional Mismatch-Provoked Excision prised of DNA helicase II and several single... break that directs repair may reside 3 or 5 to the mispair,Summary and excision removes that portion... of the incised strand spanning the nick and the mismatch (Fang and Modrich,Mismatch-provoked excision directed |
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Source: Burgers, Peter M. - Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis |
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Collection: Biology and Medicine |
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| 23 | Spectroscopic and Theoretical Insights into Sequence Effects of Aminofluorene-Induced Conformational Heterogeneity and Nucleotide Excision | ||
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Summary: . Nucleotide Excision Repair Assays NER assays were conducted using the E. coli UvrABC nuclease system. DNA... of Escherichia coli UvrABC-based nucleotide excision repair in a conformation-specific manner: i.e., greater... -Induced Conformational Heterogeneity and Nucleotide ... |
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Source: Cho, Bongsup P. - Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island |
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Collection: Biology and Medicine |
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| 24 | Annu. Rev. Biochem. 2005. 74:681710 doi: 10.1146/annurev.biochem.74.082803.133243 | ||
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Summary: by which the newly replicated strand is identified, and on excision of the replication error. CONTENTS... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 697 Strand Excision... GATC sites None - Complex Loads -clamp onto DNA RFC complex Loads PCNA, modulates excision polarity |
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Source: Erie, Dorothy A. - Department of Chemistry, University of North Carolina at Chapel Hill |
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Collection: Chemistry ; Biology and Medicine |
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| 25 | RecA Protein Piero R Bianco, University of California, Davis, California, USA | ||
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Summary: of the UvrABC endonuclease that initiates excision repair uvrB Helicase and ATPase; excision-repair nuclease... subunit B; part of the UvrABC endonuclease that initiates excision repair uvrC Excision-repair nuclease... with ... |
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Source: Kowalczykowski, Stephen C. - Section of Microbiology, University of California, Davis |
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Collection: Biology and Medicine |
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| 26 | A phosphoramidate substrate analog is a competitive inhibitor of the Tetrahymena group I ribozyme | ||
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Summary: with natural nucleic acids, including triple helices [27], and they show signi¢cant nuclease resistance [28... a cyclic phosphate intermediate, recog- nizes and cleaves the r-NP backbone. Micrococcal nuclease (S7... to cleave both d-NP and r-NP. However, nucleases P1 and S1, which both target the O3P^P bond, are not able |
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Source: Doudna, Jennifer A. - Department of Molecular and Cell Biology, University of California at Berkeley |
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Collection: Biology and Medicine |
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| 27 | An Alternative Form of Replication Protein A Expressed in Normal Human Tissues Supports DNA Repair* | ||
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Summary: , the nucleotide excision repair activity (excision nuclease) has been reconsti- tuted in vitro with purified... or stabilizing the proper assembly of the excision nuclease. Formation of these complexes may be promoted... that undertheseconditions,therateofexcisionbyaRPA-reconstituted ... |
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Source: Borgstahl, Gloria - Eppley Institute for Cancer Research, University of Nebraska Medical Center |
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Collection: Biology and Medicine |
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| 28 | JOURNAL OF VIROLOGY, Oct. 1984, p. 255-265 0022-538X/84/100255-11$02.00/0 | ||
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Summary: were eluted from low-melting-point agarose (Bio-Rad Laboratories) gels by excising the DNA fragment... with S1 nuclease. The procedure of Berk and Sharp (7) was applied. The DNA used for hybridization... of denatured calf thymus DNA-100 U of Si nuclease (Sigma) was added. The mixture was incubated at 20°C for 1 h |
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Source: Lübbert, Hermann - Fakultät für Biologie, Ruhr-Universität Bochum |
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Collection: Biology and Medicine |
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| 29 | Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases | ||
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Summary: Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases Kelly... injection of mRNAs encoding specific zinc-finger nucleases (ZFNs). Both local mutagenesis via nonhomologous... experimental organisms. Zinc-finger nucleases (ZFNs) are proving to be powerful tools for directed genome |
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Source: NONE; Rutter, Jared - Department of Biochemistry, University of Utah |
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Collection: Biology and Medicine |
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| 30 | Holliday junction-binding peptides inhibit distinct junction-processing enzymes | ||
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Summary: of Escherichia coli and interfere with the resolution of HJ substrates by the RuvABC complex. Our results suggest... and in vivo. homologous recombination recombination-dependent repair RecG helicase RuvABC resolvasome tyrosine... -planar HJ and inhibit the RecG helicase and RuvABC resolvase of Escherichia coli. Materials and ... |
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Source: Segall, Anca - Department of Biology, San Diego State University |
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Collection: Biology and Medicine |
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| 31 | Nucleic Acids 3rd period (10:10 am-11:00 am) | ||
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Summary: Detection McEachern September 15 Alteration of cloned DNA: Nucleases and site-directed mutagenesis Mc... reactions involving RNA: Nucleases Kushner September 22 Enzymatic reactions involving RNA: poly... Eachern #12;2 October 27 DNA repair: excision repair Kushner October 29 Fall Break November 1 DNA repair |
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Source: Arnold, Jonathan - Nanoscale Science and Engineering Center & Department of Genetics, University of Georgia |
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Collection: Biotechnology |
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| 32 | Annu. Rev. Genet. 2003. 37:61146 doi: 10.1146/annurev.genet.37.110801.142616 | ||
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Summary: BCD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 630 RuvABC... -strand interruptions due to excision-repair events in progress. These biologically important lesions im- pose... bases and remove them to initiate base excision repair (64, 177). Bulky base ... |
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Source: Courcelle, Justin - Department of Biology, Portland State University |
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Collection: Biology and Medicine |
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| 33 | Copyright 1998 by the Genetics Society of America Damage Control: The Pleiotropy of DNA Repair Genes in Drosophila melanogaster | ||
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Summary: characteristics and their intide adduct on an otherwise normal duplex, which can be excised by the UvrABC... of unrepaired lesionsreconstituted human excision nuclease, cleave adjacent to interstrand crosslinks... transposase excises an ele- ment from a donor site, leaving a DSB (Figure ... |
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Source: Sekelsky, Jeff - Department of Biology, University of North Carolina at Chapel Hill |
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Collection: Biology and Medicine |
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| 34 | MOLECULAR PROCESSING OF REPLICATION INTERMEDIATES IN ESCHERICHIA COLI AFTER DNA DAMAGE | ||
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Summary: then be degraded by the RecBCD helicase-nuclease or collapse to from a double strand break if RuvABC resolves... ............................................................................................... 90 A.2 Nucleotide excision repair, but not translesion DNA synthesis, is required for the recovery... ... |
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Source: Courcelle, Justin - Department of Biology, Portland State University |
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Collection: Biology and Medicine |
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| 35 | Activities of human exonuclease 1 that promote cleavage of transcribed immunoglobulin | ||
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Summary: cleavage. Excision is nick-dependent and structure-dependent. These results lead to a model for exonuclease... to class III of the RAD2 family of nucleases, which includes structure-specific nucleases like flap... , dependent on either uracil excision by UNG or mismatch recognition by MutS (17). DNA nicking ... |
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Source: Maizels, Nancy - Department of Biochemistry, University of Washington at Seattle |
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Collection: Biology and Medicine |
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| 36 | ENZYMATIC PROCESSING OF REPLICATION INTERMEDIATES THAT OCCUR FOLLOWING UV-INDUCED DNA DAMAGE IN ESCHERICHIA COLI | ||
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Summary: nucleotide excision repair. Proc Natl Acad Sci U S A 99, 1467-72. Orren, D. K. & Sancar, A. (1989). The (A)BC... that RecG and RuvABC can catalyze this reaction on synthetic DNA substrates and it has been proposed... , indicating that RuvABC and RecG are not essential for the resumption of replication. ... |
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Source: Courcelle, Justin - Department of Biology, Portland State University |
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Collection: Biology and Medicine |
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| 37 | Creating Directed Double-strand Breaks with the Ref Protein A NOVEL RecA-DEPENDENT NUCLEASE FROM BACTERIOPHAGE P1*S | ||
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Summary: Creating Directed Double-strand Breaks with the Ref Protein A NOVEL RecA-DEPENDENT NUCLEASE FROM... A-dependent nuclease. Ref binds to ss- and dsDNA but does not cleave any DNA substrate until RecA protein and ATP... as a co-nuclease in the Ref/RecA system. Ref nuclease activity can be limited to the targeted ... |
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Source: Cox, Michael M. - Department of Biochemistry, University of Wisconsin at Madison |
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Collection: Biology and Medicine |
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| 38 | JOURNAL OF BACTERIOLOGY, Mar. 1996, p. 13471350 Vol. 178, No. 5 0021-9193/96/$04.00 0 | ||
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Summary: more readily than a CPD, which may indicate a greater affinity of the excision repair apparatus for (6... following irradiation. This may be a result of a greater affinity of the Uvr(A)BC nucleotide excision repair... - nuclease V to incise bacterial DNA specifically at the sites of CPDs and found about 80% repair in 20 min |
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Source: Courcelle, Justin - Department of Biology, Portland State University |
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Collection: Biology and Medicine |
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| 39 | Molecular Cell 23, 133142, July 7, 2006 2006 Elsevier Inc. DOI 10.1016/j.molcel.2006.06.005 The Exonuclease TREX1 Is in the SET Complex | ||
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Summary: A-treated cells as well as a specific inhibitor of the nuclease. The GzmA-activated endonuclease is NM23- H1... 1 endo- nuclease is activated. The importance of NM23-H1 and SET in GzmA-mediated cell death... endonuclease re- sponsible for base excision repair, Ape1 (Fan et al., 2003b), and the DNA-binding protein HMG |
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Source: Lieberman, Judy - Immune Disease Institute, Harvard University |
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Collection: Biology and Medicine |
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| 40 | JOURNAL OF BACTERIOLOGY, Feb. 2010, p. 665673 Vol. 192, No. 3 0021-9193/10/$12.00 doi:10.1128/JB.01018-09 | ||
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Summary: Characterization of the Nucleotide Excision Repair System of Neisseria gonorrhoeae Brian E. LeCuyer, Alison K... excision repair (NER) is universally used to recognize and remove many types of DNA damage. In eubacteria... , nucleotide excision repair (NER), recombination repair, and replication restart (10, 18). Among the ... |
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Source: Seifert, Hank - Department of Microbiology-Immunology, Northwestern University |
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Collection: Biology and Medicine |
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| 41 | Supporting Information DNA-Templated Polymerization of Side-Chain-Functionalized Peptide Nucleic Acid | ||
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Summary: (for lane 13 in Figure 3a, the fastest migrating band was excised; the major bands were excised... for the other samples) were visualized by UV shadowing and excised. The excised polyacrylamide was homogenized... in 19 µL 1x S1 nuclease reaction buffer (Promega) and 1 µL S1 ... |
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Source: Liu, David R. - Department of Chemistry and Chemical Biology, Harvard University |
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Collection: Chemistry ; Biology and Medicine |
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| 42 | . The EMBO Journal vol.14 no.1 pp.17&-187, 1995 -S ^ In vitro RNA editing-like activity in a mitochondrial | ||
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Summary: was obtained. Inliibition by micrococcal nuclease suggests a requirement for some type of endogenous RNA... within the 5' RNase H fragment. Nuclease PI digestion of the 5' fragment of pNB2 RNA labeled with [a... and the oligomer S165. The S' fragment was gel-isolated, digested with IWase T2 (A) or nuclease PI (B |
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Source: Simpson, Larry - Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles |
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Collection: Biology and Medicine |
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| 43 | Principles that Govern the Folding of Protein Chains Author(s): Christian B. Anfinsen | ||
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Summary: received from the SwedishRoyalAcademyof Sciences specifically cites "... studies on ribo- nuclease... staphylococcal nuclease .The origi- molecule, but I will first summarize this conclu- some of the older... , by excision of two internal bits of se- quence. The elegant studies of Steiner and his |
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Source: Mittal, Aditya - School of Biological Sciences, Indian Institute of Technology, Delhi |
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Collection: Biotechnology ; Biology and Medicine |
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| 44 | MOLECULAR AND CELLULAR BIOLOGY, Dec. 2003, p. 85058518 Vol. 23, No. 23 0270-7306/03/$08.00 0 DOI: 10.1128/MCB.23.23.85058518.2003 | ||
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Summary: -PK) regulates the DNA damage introduced during Sleeping Beauty (SB) element excision and reinsertion... excision were efficiently and accurately processed by the major Ku-dependent nonhomologous... transposable elements were not found to be efficiently circularized after transposase-mediated excision, DNA |
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Source: Kay, Mark A. - Department of Genetics, Stanford University |
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Collection: Biology and Medicine |
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| 45 | Copyright 2002 by the Genetics Society of America Efficient Mobilization of mariner in Vivo Requires Multiple Internal Sequences | ||
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Summary: ABSTRACT Aberrant products of mariner excision that have an impaired ability to be mobilized often include... binding sites. The isolation of excision-defective elements with point mutations at nucleotide positions... of type II transposons (DNA transpo- nuclease degradation (Staveley et al. 1995), therebysons |
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Source: Hartl, Daniel L. - Department of Organismic and Evolutionary Biology, Harvard University |
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Collection: Environmental Sciences and Ecology ; Biology and Medicine |
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| 46 | This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research | ||
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Summary: potential biotechnologies, such as zinc finger nucleases, that could be also used for transgene excision... 's personal copy Keeping the genie in the bottle: transgene biocontainment by excision in pollen Hong S. Moon1... . Site- specific recombination systems could allow highly effi- cient excision of ... |
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Source: Dai, Pengcheng - Department of Physics and Astronomy, University of Tennessee |
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Collection: Materials Science ; Physics |
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| 47 | A human nuclease specific for G4 DNA , Akiko Yabuki* | ||
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Summary: A human nuclease specific for G4 DNA Hui Sun* , Akiko Yabuki* , and Nancy Maizels*§¶ Departments... 9, 2001) We have identified a human nuclease that specifically cleaves four-stranded DNA stabilized... by G quartets (G4 DNA). This nucle- ase, GQN1 (G quartet nuclease 1), cuts within the single |
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Source: Maizels, Nancy - Department of Biochemistry, University of Washington at Seattle |
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Collection: Biology and Medicine |
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| 48 | THE RECOVERY OF DNA REPLICATION IN ESCHERICHIA COLI IRRADIATED WITH ULTRAVIOLET LIGHT | ||
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Summary: Chapter 3.Recovery of DNA replication in UV-irradiated Escherichia coli requires both excision repair... ArecA double mutant (figure 2A). Since uvrA mutants are unable to excise UV-induced lesions, they hypothesized... of the uvrA and uvrB genes for excision repair and the umuCD genes for bypassing replication blocking DNA |
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Source: Courcelle, Justin - Department of Biology, Portland State University |
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Collection: Biology and Medicine |
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| 49 | Molecular Microbiology (2001) 40(1), 257269 BldD is a direct regulator of key developmental genes in | ||
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Summary: of mutation in bldD on the expression of these three target genes was assessed using S1 nuclease protection... al., 1987), an ATP-binding cassette (ABC) membrane-spanning transporter (bldK; Nodwell et al., 1996... implicated in signal uptake. bldK is a complex locus that encodes an ABC transporter, and bldK mutants |
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Source: Elliot, Marie A. - Department of Biology, McMaster University |
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Collection: Biology and Medicine |
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| 50 | Interstrand crosslink repair: can XPF-ERCC1 be let off the hook? | ||
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Summary: replication protein A positions nucleases in nucleotide excision repair. Genes Dev. 12, 25982609 27 Thompson... that the endonuclease xeroderma pigmentosum complementation group F-excision repair cross-comple- menting rodent repair... deficiency complementation group 1 (XPF-ERCC1) has been heretofore misplaced. During ... |
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Source: Sekelsky, Jeff - Department of Biology, University of North Carolina at Chapel Hill |
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Collection: Biology and Medicine |
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| 51 | Vol. 10, No. 11 Nucleosome Depletion Alters the Chromatin Structure of | ||
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Summary: nuclease-resistant chromatin core of approximately 200 base pairs of DNA. The structure of the centromere... exhibit altered chromatin structures in vivo. Thus the structure, as delineated by nuclease digestion... the cell incapable of chromosome segregation. Histone repression resulted in increased nuclease sensitivity |
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Source: Bloom, Kerry S.- Department of Biology, University of North Carolina at Chapel Hill |
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Collection: Biology and Medicine |
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| 52 | Domain Mapping of Human Apurinic/Apyrimidinic Endonuclease STRUCTURAL AND FUNCTIONAL EVIDENCE FOR A DISORDERED AMINO TERMINUS AND A TIGHT | ||
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Summary: the nuclease fold with restoration of globular conformation for the carboxyl two-thirds of the molecule. Of all... of the nuclease fold, there must be multiple means by which DNA containing an abasic site associates... similarity, but also the tertiary structure of the putative active site (the nuclease fold) is conserved (5 |
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Source: Strauss, Phyllis R. - Department of Biology, Northeastern University |
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Collection: Biology and Medicine |
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| 53 | JOURNAL OF BACTERIOLOGY, Aug. 2007, p. 54525462 Vol. 189, No. 15 0021-9193/07/$08.00 0 doi:10.1128/JB.00408-07 | ||
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Summary: be repaired by nucleotide excision repair or bypassed by translesion DNA polymerases (7, 9, 10, 13, 15... , and are significantly reduced in ruvABC mutants, which lack an enzyme complex that catalyzes branch migration... that following replication arrest in dnaB(Ts) mutants, RuvABC catalyzes the displacement and regression |
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Source: Courcelle, Justin - Department of Biology, Portland State University |
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Collection: Biology and Medicine |
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| 54 | Distinct Roles of Two Mg2+ Binding Sites in Regulation of Murine Flap | ||
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Summary: ) and MutH (36) nucleases, which are involved in many DNA repair pathways, including nucleotide excision... + or Mn2+, are required for the active center of FEN-1 nucleases. However, it remains unclear as to how Mg... nuclease activities in a mode different from the "two-metal mechanism". Murine flap ... |
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Source: Krishnamoorthi, Ramaswamy - Department of Biochemistry, Kansas State University |
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Collection: Biology and Medicine |
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| 55 | DNA Repair 8 (2009) 803812 Contents lists available at ScienceDirect | ||
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Summary: of the FHA domain. 3.3. Synthesis-dependent strand annealing in nbs mutants We used a P element excision... instead of red eyes. Excision of the P{wa} element was carried out in males by crossing in P transposase... . Excision generates a 14-kb gap relative to the sister chromatid, which is the only template for HR repair |
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Source: Sekelsky, Jeff - Department of Biology, University of North Carolina at Chapel Hill |
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Collection: Biology and Medicine |
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| 56 | www.landesbioscience.com Fly 1 Fly 4:2, 1-4;April/May/June 2010; 2010 Landes Bioscience | ||
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Summary: excision for making null mutations in Drosophila. Genetics 2009; 183:118793; PMID: 19687136; DOI: 10... are frequently utilized in imprecise excision screens to generate deletions in genes of interest. In general... restored P elements, internally deleted P elements, precise excisions, or repair events that delete DNA |
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Source: McVey, Mitch - Department of Biology, Tufts University |
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Collection: Biology and Medicine |
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| 57 | 2000 Macmillan Magazines Ltd NATURE CELL BIOLOGY | VOL 2 | JULY 2000 | www.nature.com/ncb 399 | ||
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Summary: .nature.com/ncb 399 ABC1 promotes engulfment of apoptotic cells and transbilayer redistribution of phosphatidylserine... -binding-cassette transporter 1 (ABC1) has been implicated in processes related to membrane-lipid turnover. Here, using in vivo... loss-of-function and in vitro gain-of-function models, we show that ABC1 ... |
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Source: Institut de Biologie Physico-Chimique CNRS, Physico-Chimie Moléculaire des Membranes Biologiques |
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Collection: Chemistry ; Biology and Medicine |
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| 58 | Distinct Activities of Exonuclease 1 and Flap Endonuclease 1 at Telomeric G4 DNA | ||
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Summary: endonuclease 1 (FEN1) are members of the RAD2 family of structure-specific nucleases. Genetic analysis has... Exonuclease 1 (EXO1) and Flap endonuclease 1 (FEN1) belong to the RAD2 family of structure-specific nucleases... . They share a core nuclease domain that is remarkably conserved from yeast to mammals [1], and display both ... |
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|
Source: Maizels, Nancy - Department of Biochemistry, University of Washington at Seattle |
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Collection: Biology and Medicine |
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| 59 | Samenvatting en algemene discussie Het DNA, de drager van alle genetische informatie, wordt constant bedreigd | ||
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Summary: laatstgenoemde klassen van herstelmechanismen zijn base-excision herstel (BER), UV damage endonuclease (UVDE... herkennen en het DNA te knippen: UvrA, UvrB en UvrC. Het moleculaire mechanisme van het UvrABC systeem... C is de nuclease van het systeem en knipt aan weerszijden van de schade zodra de aanwezigheid van de |
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|
Source: van den Brink, Jeroen - Leiden Institute of Physics, Universiteit Leiden |
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Collection: Physics |
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| 60 | Cell, Vol. 90, 10311039, September 19, 1997, Copyright 1997 by Cell Press The Transmembrane Kinase Ire1p Is a | ||
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Summary: activation of an unknown nuclease. In the absence of the second step in the splicing reaction (ligation... RNA, to a constitutively active endo- after binding to the unfolded protein response element nuclease (such as t... Has Both Kinase and Nuclease Activity In Vitro (A) Amino-acid sequence alignment of the C |
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|
Source: Walter, Peter - Department of Biochemistry and Biophysics, University of California at San Francisco |
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Collection: Biology and Medicine |
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| 61 | Copyright 2007 by the Genetics Society of America DOI: 10.1534/genetics.106.070052 | ||
|
Summary: ), and defects in repair of DSBs generated by excision of transposable elements (Boyd et al. 1981; Beall and Rio... generated by P-element excision (reviewed in Adams and Sekelsky 2002). P{EPgy2}mus309EY03745 harbors a P... of mus309 mutations in these homozygotes (data not shown). A total of 759 excisions were screened by PCR |
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|
Source: McVey, Mitch - Department of Biology, Tufts University; Sekelsky, Jeff - Department of Biology, University of North Carolina at Chapel Hill |
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Collection: Biology and Medicine |
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| 62 | Sequence effects of aminofluorene-modified DNA duplexes: thermodynamic and circular | ||
|
Summary: are elimi- nated by NER (3,12,17). The AF-lesion excision efficiency by the E.coli UvrABC system is known... and incision by UvrABC nuclease. Biochemistry, 42, 1265412661. 12. Singer,B. and Hang,B. (2000) Nucleic acid... ,L.J. (1998) Rate of incision of N-acetyl-2-aminofluorene and N-2-aminofluorene adducts ... |
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|
Source: Cho, Bongsup P. - Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island |
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Collection: Biology and Medicine |
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| 63 | J. Mol.Biol. (1985) 185,625-637 Bacterial DNA Topoisomerase I Can Relax Positively | ||
|
Summary: section (see the legend to Fig. 4). Restriction sites used in excising and labeling fragments are Bam... reaction mixtures. Staphylococcal nuclease footprints were performed in the same way, except that a 5 mg... /ml Staphylococcal nuclease stock solution was diluted to 25 pg/ml before the addition of 1 gl to the reaction |
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|
Source: Kirkegaard, Karla - Department of Microbiology and Immunology, Stanford University |
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Collection: Biology and Medicine |
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| 64 | nature structural biology volume 5 number 8 august 1998 707 1Structural Biology Center, Korea Institute of Science & Technology, P.O. Box 13, Cheongryang, Seoul 130-650, South Korea. 2Both authors contributed equally to the work. | ||
|
Summary: , also called RAD27/RTH1/MF-1/DNase IV), a structure specific nuclease with 5' flap DNA endonuclease... , proliferating cell nuclear antigen physically interacts with FEN-1 and stimu- lates its nuclease activity by 10... endonuclease-1 (FEN-1), a structure specific nuclease, is an essential enzyme for eukaryotic DNA replication |
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|
Source: Cho, Yunje - Department of Life Science, Pohang University of Science and Technology |
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Collection: Biology and Medicine |
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| 65 | RuvABC Is Required to Resolve Holliday Junctions That Accumulate following Replication on Damaged Templates | ||
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Summary: RuvABC Is Required to Resolve Holliday Junctions That Accumulate following Replication on Damaged... 97207 RuvABC is a complex that promotes branch migration and resolution of Holliday junctions. Although... ruv mutants are hypersensitive to UV irradiation, the molecular event(s) that necessitate RuvABC |
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|
Source: Courcelle, Justin - Department of Biology, Portland State University |
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Collection: Biology and Medicine |
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| 66 | Molecular Cell, Vol. 15, 607620, August 27, 2004, Copyright 2004 by Cell Press The Fanconi Anaemia Gene FANCC Promotes | ||
|
Summary: DNA damage. Here, we use the model vertebrate cell excision repair nucleases ERCC1 and XPF results... substrate of after the initial excision step. Studies in budding yeast point to a similar situation. Mutants... in the Rad3 (deficientthis nuclear complex is the downstream FA protein in nucleotide excision ... |
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|
Source: Patel, KJ - Laboratory of Molecular Biology, MRC |
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Collection: Biology and Medicine |
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| 67 | Comparative Biochemistry and Physiology Part C 136 (2003) 343356 1532-0456/03/$ -see front matter 2003 Elsevier Inc. All rights reserved. | ||
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Summary: al., 1995a,b,c), and the toxicity of the recom- binant enzyme has been extended to oocyte mat... , respectively, of a ribonuclease. Both testes with their epididymides and seminal vesicles were excised after 20... with collagen fibres, fibroblasts, and fibrocytes was included in the histological assessment of excised testes |
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|
Source: Raines, Ronald T. - Departments of Biochemistry & Chemistry, University of Wisconsin at Madison |
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Collection: Chemistry ; Biology and Medicine |
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| 68 | ITCHY Libraries 129 From: Methods in Molecular Biology, vol. 231: Directed Evolution Library Creation: Methods and Protocols | ||
|
Summary: are prepared by treatment with a single-strand nuclease and a DNA polymerase. This synchronized truncation... - rated S-dNMP (6). Blunt ends are prepared by treatment with a single-strand nuclease and a DNA... .6 mM MgCl2. 7. Mung Bean Nuclease. 8. 10X Mung bean buffer: 500 mM sodium acetate, pH 5.0, 300 mM Na |
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|
Source: Gray, Jeffrey J. - Department of Biomolecular and Chemical Engineering, Johns Hopkins University; Ostermeier, Marc - Department of Biomolecular and Chemical Engineering, Johns Hopkins University |
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Collection: Biology and Medicine ; Biotechnology ; Chemistry |
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| 69 | The EMBO Journal vol.7 no. 1 1 pp.3559 -3569, 1988 Ribosome pausing and stacking during translation of a | ||
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Summary: . Translating extracts are treated with micrococcal nuclease at a concentration sufficient to trim away portions... .Walter nuclease Iextract mRNA fragments fragments (steps D and E). Because this polymerase, in the absence... are treated with nuclease to trim away fragments of RNA that are not associated with ribosomes (A |
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|
Source: Walter, Peter - Department of Biochemistry and Biophysics, University of California at San Francisco |
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Collection: Biology and Medicine |
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| 70 | Cytogenet Genome Res 107:172179 (2004) DOI: 10.1159/000080595 | ||
|
Summary: com- plex of human DNA repair excision nuclease. J Biol Chem 230:2265722660 (1995). Riechmann V... -mail: sekelsky@unc.edu ABC Fax + 41 61 306 12 34 E-mail karger@karger.ch www.karger.com © 2004 S. Karger AG... -9 is the Drosophila ortholog of the nucleotide excision repair (NER) endonuclease XPF (human |
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|
Source: Sekelsky, Jeff - Department of Biology, University of North Carolina at Chapel Hill |
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Collection: Biology and Medicine |
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| 71 | New Pathways in Folding of the Tetrahymena Group I Rick Russell and Daniel Herschlag* | ||
|
Summary: of the periph- eral element P5abc. This, along with other results, has suggested that P5abc may serve... cleavage activity as a readout for native state formation and investigate the effect of P5abc on the rate... of folding to the native structure. Despite the early folding of P5abc, its removal to give the ... |
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|
Source: Herschlag, Dan - Department of Biochemistry, Stanford University |
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Collection: Biology and Medicine |
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| 72 | www.landesbioscience.com Cell Cycle 221 [Cell Cycle 4:2, 221-224; February 2005]; 2005 Landes Bioscience | ||
|
Summary: polymerase, Okazaki fragment, idling, nuclease, nick translation, FEN1, PCNA ABBREVIATIONS Pol , DNA... metabolic processes, such as base excision repair, that depend on Pol for synthesis. INTRODUCTION... maturation where it degrades the initiator RNA.8 However, FEN1 also functions in long patch base-excision |
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|
Source: Burgers, Peter M. - Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis |
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|
Collection: Biology and Medicine |
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| 73 | Genome sequence of Halobacterium species NRC-1 Wailap Victor Nga,b | ||
|
Summary: appear to be complete as copies of the uvrABC nuclease and uvrD helicase, and endonucleases... . Accordingly, NRC-1 has multiple active K transporters, includ- ing KdpABC, an ATP-driven K transport system... described previously (41). Interestingly, Kd- pABC, TrkA (three of five copies), and NhaC (one of ... |
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|
Source: Lowe, Todd M. - Department of Biomolecular Engineering, University of California at Santa Cruz |
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|
Collection: Biology and Medicine ; Biotechnology |
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| 74 | JOURNAL OF VIROLOGY, 0022-538X/00/$04.00 0 | ||
|
Summary: repair or base excision repair similarly require a polymerase, a nuclease, and a ligase to repair gaps... , or reverse transcriptase), a nuclease (flap endonuclease), and a ligase (ligase I, III, or IV and its... , a nuclease to remove the 5 flap, and a ligase to join the strands (Fig. 1A). Repair pathways ... |
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|
Source: Bushman, Frederic - Department of Microbiology, University of Pennsylvania |
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|
Collection: Biology and Medicine |
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| 75 | Annu. Rev. Genet. 2001. 35:5382 Copyright c 2000 by Annual Reviews. All rights reserved | ||
|
Summary: of DNA metabolism, and an illuminating one. Replication fork repair can take its place with excision... repair, mismatch repair, base excision re- pair, and direct repair [such as the reaction catalyzed by DNA... of replication forks functions to maintain genome integrity. Most DNA repair processes (such as excision repair |
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|
Source: Cox, Michael M. - Department of Biochemistry, University of Wisconsin at Madison |
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|
Collection: Biology and Medicine |
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| 76 | Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection | ||
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Summary: Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection Yoko... variant of gene targeting uses direct microinjection of custom-designed Zinc Finger Nuclease (ZFN) m... finger nucleases (ZFNs), which are * Corresponding author. Tel.: þ420 387775283; fax: þ420 385310354. E |
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|
Source: urovec, Michal - Department of Physiology, University of South Bohemia |
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|
Collection: Biology and Medicine |
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| 77 | Proc. Nati, Acad. Sci. USA Vol. 79, pp. 4030-4034, July 1982 | ||
|
Summary: RNA and S1 Nuclease Analysis. Totalcellular RNA was prepared as described by Favaloro et aL (13) except... the text. Excised segment in pBRdrepresents the deletionthatremoves sequences that inhibit SV40-pBR322... gene. H, Hindm; B, BamHI. S1 nuclease analysis (13, 14) the antisense strand ofa Hae III/ HinfI ,B |
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|
Source: DiMaio, Daniel - Departments of Genetics & Molecular Biophysics and Biochemistry, Yale University |
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|
Collection: Biology and Medicine |
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| 78 | Genomic demography: a life-history analysis of transposable element evolution | ||
|
Summary: 1998; Jordan & McDonald 1998, 1999a,b,c; Kim et al. 1998). Knowledge of transposition and excision... ^transposon coevolution. Evolutionary change in Ty elements depends on their replication and excision rates, which have... /2 families, we develop a novel `genomic demography' model to estimate long-term transposition and ... |
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|
Source: Promislow, Daniel - Department of Genetics, University of Georgia |
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|
Collection: Biology and Medicine |
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| 79 | Annu. Rev. Genet. 2001. 35:24374 Copyright c 2001 by Annual Reviews. All rights reserved | ||
|
Summary: , as assayed by the accessibility of micro- coccal nuclease to the DNA in isolated chromatin. This alteration... . The recB1080 (D1080A) mutation, which abolishes the nuclease activities (132), blocks RecA loading (3, 5... ), suggesting that some aspect of the Chi-nuclease interaction must precede RecA loading. In accord with a ... |
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|
Source: Smith, Gerry - Basic Sciences Division, Fred Hutchinson Cancer Research Center |
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|
Collection: Biology and Medicine |
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| 80 | Molecular Microbiology (2003) 50(2), 475486 doi:10.1046/j.1365-2958.2003.3728.x 2003 Blackwell Publishing Ltd | ||
|
Summary: is taken up via the bldK-specified ABC transporter (Nodwell et al., 1996; Nodwell and Losick, 1998... delayed produc- tion of Red (Takano et al., 2001). We analysed adpAc expression by S1 nuclease mapping... eliminating g-butyrolactone production in liquid cultures. A. S1 nuclease mapping of the transcriptional start |
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|
Source: Bibb, Mervyn - Department of Molecular Microbiology, John Innes Centre; Buttner, Mark - Department of Molecular Microbiology, John Innes Centre |
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|
Collection: Biology and Medicine |
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| 81 | Z .Mutation Research 459 2000 219228 www.elsevier.comrlocaterdnarepair | ||
|
Summary: and characterization of the XPFERCC1 complex of human Z .DNA repair excision nuclease, J. Biol. Chem. 230 1995 22657... .elsevier.comrlocatermutres Nucleotide excision repair endonuclease genes in Drosophila melanogaster Jeff J. Sekelsky a,b,) , Katheryn J... .Nucleotide excision repair NER is the primary ... |
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|
Source: Sekelsky, Jeff - Department of Biology, University of North Carolina at Chapel Hill |
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|
Collection: Biology and Medicine |
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| 82 | Stimulation of RTH1 Nuclease of the Yeast Saccharomyces cereVisiae by Replication Protein A | ||
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Summary: Stimulation of RTH1 Nuclease of the Yeast Saccharomyces cereVisiae by Replication Protein A Esther... Vember 22, 1996; ReVised Manuscript ReceiVed March 19, 1997X ABSTRACT: The RTH1 nuclease is involved... in the function of RTH1 nuclease, including its structure specific endonuclease activity. Initial in Vitro studies |
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|
Source: McQuade, D. Tyler - Department of Chemistry and Biochemistry, Florida State University |
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|
Collection: Chemistry |
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| 83 | Q 1992 by The AmericanSociety for Biochemistry and MolecularBiology, Inc. OFBIOLOGICAL CHEMISTRY Vol. 267, No. 11, Issue of April 15,pp. 7683-7689,1992 | ||
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Summary: ; SDS, sodium dodecyl sulfate; LB, Luria broth;TB, terrific broth; SN,staphylococ- cal nuclease; PMSF... of the transmembranedomainofGpA. This domain is fusedto the C terminus of staphylococcal nuclease via a flexible linker... nuclease Q149P (pPCV-I) was kindly suppliedby A. Frankel (WistarInstitute).Restriction enzymes, T4 DNA |
|||
|
Source: Engelman, Donald M.- Department of Molecular Biophysics and Biochemistry, Yale University |
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|
Collection: Biology and Medicine |
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| 84 | The role of tryptophan in staphylococcal nuclease stability Hong-Yu Hu a | ||
|
Summary: The role of tryptophan in staphylococcal nuclease stability Hong-Yu Hu a , Ming-Chya Wu a,b,c... : Staphylococcal nuclease W140A F61W/W140A Y93W/W140A Tryptophan modification Local hydrophobicity Staphylococcal... nuclease (SNase) has a single Trp residue at position 140. Circular dichroism, intrinsic and ANS |
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|
Source: Academia Sinica (Taiwan), Institute of Physics, Laboratory of Statistical and Computational Physics |
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|
Collection: Physics |
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| 85 | Supporting Information For: Removal of Covalent Heterogeneity Reveals Simple Folding Behavior for P4-P6 RNA | ||
|
Summary: standard P1 nuclease reaction conditions involving a 30 minute incubation with 1x P1 (see Experimental... ) and bovine serum albumin (BSA) were purchased from New England BioLabs (MA). P1 Nuclease from Penicillium... TAT A #12;S13 Native Gel Assays and P1 Nuclease Digestions P4-P6 was transcribed from a double |
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|
Source: Herschlag, Dan - Department of Biochemistry, Stanford University |
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|
Collection: Biology and Medicine |
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| 86 | Modeling of Flap Endonuclease Interactions with DNA Substrate | ||
|
Summary: of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037 USA Structure-specific 50 nucleases... -specific 50 nucleases. q 2003 Elsevier Science Ltd. All rights reserved Keywords: structure-specific 50... nuclease; FEN1; point mutation; methylphosphonate; 20 -O-methyl*Corresponding author Introduction Structure |
|||
|
Source: Onufriev, Alexey - Departments of Computer Science & Physics, Virginia Tech |
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|
Collection: Physics ; Biology and Medicine |
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| 87 | RESEARCH ARTICLE Open Access Characterization of a Mycobacterium smegmatis | ||
|
Summary: sleeves": DNA damage recognition by the UvrABC nuclease system. Mutat Res 2005, 577:92-117. 15. Kurthkoti... of the Nucleotide Excision Repair system (NER). The two mutants showed identical phenotypes, although the respective... excision repair system (NER) and is highly conserved among mycobacteria. NER ... |
|||
|
Source: Paris-Sud XI, Université de - Institut d'Optique, Laboratoire Charles-Fabry, Groupe d'Optique Atomique |
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|
Collection: Physics |
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| 88 | COMMUNICATION Identification of the RecA Protein-loading Domain of | ||
|
Summary: helicase, and an ATP-depen- dent nuclease. The enzyme initiates the process of homologous recombination... to be productive in recombination, the destructive nuclease activity must be carefully controlled. w exerts its... degradation of DNA by regu- lating the enzyme's asymmetric nuclease activities (Anderson & ... |
|||
|
Source: Kowalczykowski, Stephen C. - Section of Microbiology, University of California, Davis |
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Collection: Biology and Medicine |
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| 89 | Xpf and Not the Fanconi Anaemia Proteins or Rev3 Accounts for the Extreme Resistance to Cisplatin in | ||
|
Summary: excision repair nuclease subcomponent XPF is essential for crosslink repair The fact that Dictyostelium... ). In summary, the excision repair nuclease subcomponent xpf is essential for repairing crosslinks... for crosslink repair all involve a nuclease(s) carrying out the ... |
|||
|
Source: Babu, M. Madan - Laboratory of Molecular Biology, MRC; Patel, KJ - Laboratory of Molecular Biology, MRC |
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|
Collection: Biology and Medicine ; Biotechnology |
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| 90 | DNA Unwinding Step-size of E. coli RecBCD Helicase Determined from Single Turnover Chemical | ||
|
Summary: DNA exo- nuclease, ssDNA endonuclease, DNA-dependent ATPase, and helicase activities.11,12 Through a com... DNA end. The enzyme shows no detectable nuclease activity on circular dsDNA; potent nuclease activity... helicase involved in methyl-directed mismatch repair29 and nucleotide excision repair,30,31 ... |
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|
Source: Cistola, David P. - Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis; Lohman, Timothy M. - Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis; Smith, Gerry - Basic Sciences Division, Fred Hutchinson Cancer Research Center |
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|
Collection: Biology and Medicine |
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| 91 | JOURNAL OF BACTERIOLOGY, 0021-9193/99/$04.00 0 | ||
|
Summary: -Irradiated Escherichia coli Requires both Excision Repair and RecF Protein Function JUSTIN COURCELLE,* DAVID J. CROWLEY... that the disrupting lesions be removed. In the absence of excision repair, recF cells protect the nascent DNA... replication at UV doses from which wild-type cells recover efficiently, suggesting that recF and ... |
|||
|
Source: Courcelle, Justin - Department of Biology, Portland State University |
|||
|
Collection: Biology and Medicine |
|||
| 92 | TB261 02/00 Novagen 1United States & Canada 800-207-0144 Germany 0800 6931 000 | ||
|
Summary: Benzonase ® Nuclease TB261 02/00 Novagen 1United States & Canada 800-207-0144 Germany 0800 6931 000... U it d Ki d 0800 622935 Benzonase Nuclease Benzonase Nuclease, Purity >99% 10,000 U 70664... -3 Benzonase Nuclease, Purity >90% 10,000 U 70746-3 Benzonase is a genetically engineered endonuclease ... |
|||
|
Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem |
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|
Collection: Biotechnology ; Biology and Medicine |
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| 93 | 84 BIOGENESIS AND ASSEMBLY OF MEMBRANE PROTEINS [6] that incorporation of radioactivity into hot trichloroacetic acid-insoluble | ||
|
Summary: by severance of the great vessels at the base of the heart or excision of the heart, and the pancreas... in another 50- ml aliquot of buffer A on ice. According to the size of the dog, the excised pancreas weighed... of load to cushion was about 3 : 1. The resulting pellet was resuspended in 20 ml of buffer B. Nuclease |
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|
Source: Walter, Peter - Department of Biochemistry and Biophysics, University of California at San Francisco |
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|
Collection: Biology and Medicine |
|||
| 94 | Copyright 2004 by the Genetics Society of America RuvAB and RecG Are Not Essential for the Recovery of DNA Synthesis Following | ||
|
Summary: is incorporated, rearrangement if excision repair or bypassed by translesion DNA polymer- replication resumes from... the rep- repaired by nucleotide excision repair (Courcelle et lication fork DNA and limiting... the degradation of the al. 1999, 2003). Cells deficient in nucleotide excision nascent DNA by RecJ and Rec |
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|
Source: Courcelle, Justin - Department of Biology, Portland State University |
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|
Collection: Biology and Medicine |
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| 95 | Genome Dyn Stab (3) R. Egel, D.-H. Lankenau: Recombination and Meiosis | ||
|
Summary: by the Spo11 nuclease, a meiosis-specific protein with homology to the Top6A subunit of archaeal type IIB... -terminal phosphoesterase motif that provides the single catalytic center for all its nuclease activities (see below... ). This nuclease func- tion is essential for Mre11 in meiosis, but in vegetative (somatic) cells, the ... |
|||
|
Source: Heyer, Wolf-Dietrich - Sections of Microbiology & Molecular and Cellular Biology, University of California, Davis |
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|
Collection: Biology and Medicine |
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| 96 | Molecular Cell, Vol. 10, 15031509, December, 2002, Copyright 2002 by Cell Press Drosophila MUS312 Interacts with the | ||
|
Summary: - cation and characterization of the XPF-ERCC1 complex of human DNA repair excision nuclease. J. Biol. Chem... Interacts with the Nucleotide Excision Repair Endonuclease MEI-9 to Generate Meiotic Crossovers tains two... ). These proteins share a highly conserved nuclease domain (Aravind et al., 1999). A ... |
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|
Source: Sekelsky, Jeff - Department of Biology, University of North Carolina at Chapel Hill |
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|
Collection: Biology and Medicine |
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| 97 | BATES, GINGER MUSE. Characterization of the protein component of Methanococcus jannaschii RNase P. (Under the direction of James W. Brown.) | ||
|
Summary: to nuclease treatment, whereas the holoenzyme from S. acidocaldarius has a buoyant density of 1.27g... /ml suggesting that it is largely made of protein and is insensitive to nuclease treatment (14,33). These two... in the form of a DEAD box helicase motif seen in ABC transporters (34). Organellar RNase P Of all RNase Ps |
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|
Source: Brown, James W. - Department of Microbiology, North Carolina State University |
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|
Collection: Environmental Sciences and Ecology ; Biology and Medicine |
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| 98 | SURVEY AND SUMMARY Comparative analysis of editosome proteins in | ||
|
Summary: , contain two putative nuclease motifs. Thus, editosomes contain a plethora of nucleases or proteins presum... - ably derived from nucleases. Five additional related proteins, three of which have zinc ®ngers, each... are involved in eukaryotic base excision repair. The critical residues in DNA polymerase b ... |
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|
Source: Schnaufer, Achim - Institute of Immunology and Infection Research, University of Edinburgh |
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Collection: Biology and Medicine |
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| 99 | SOLiDTM Total RNA-Seq Kit For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. | ||
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Summary: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24 Expected lengths of the insert and PCR product according to excised cDNA length . 25 Amplify the c... DNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 Expected lengths of the insert and PCR product ... |
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Source: Dratz, Edward A. - Department of Chemistry and Biochemistry, Montana State University |
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Collection: Biology and Medicine ; Chemistry |
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| 100 | Intersubunit signaling in RecBCD enzyme, a complex protein machine regulated by Chi hotspots | ||
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Summary: RecBCD helicase-nuclease, a paradigm of complex protein machines, initiates homologous genetic... that, in the mutant enzymes when RecD reaches the DNA end, it signals RecB's nuclease domain to cut... to the nuclease domain of RecB (Singleton et al., 2004). The steps between Chi recognition and alteration |
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Source: Smith, Gerry - Basic Sciences Division, Fred Hutchinson Cancer Research Center |
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Collection: Biology and Medicine |
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