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Sample search results for: abc excision nuclease

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1 Communications Conformation-Specific Recognition of Carcinogen-DNA Adduct in
 

Summary:  . Nucleotide Excision Assays. The 5-terminally labeled DNA substrates (2 nM) were incised by UvrABC (UvrA, 15 n... adducts by UvrABC nuclease is adduct- and sequence-specific: comparison of the rates of UvrABC nuclease... nucleotide excision ...

  

Source: Cho, Bongsup P. - Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island

 

Collection: Biology and Medicine

 
2 Diversity visualization by endonuclease: A rapid assay to monitor diverse nucleotide libraries
 

Summary:  Theam Soon Lim a,b,c , Tatjana Schütze a,b , Hans Lehrach a , Jörn Glökler a , Zoltán Konthur a... 2010 Available online 23 December 2010 Keywords: S1 nuclease Diversity Assay Nucleotide library Phage... nuclease. The assay involves only a lim- ited number of steps and can be performed in less than 4 h

  

Source: Konthur, Zoltán - Max-Planck-Institut für molekulare Genetik

 

Collection: Biology and Medicine

 
3 Identification of BFN1, a Bifunctional Nuclease Induced during Leaf and Stem Senescence in Arabidopsis1
 

Summary:  Identification of BFN1, a Bifunctional Nuclease Induced during Leaf and Stem Senescence... ­1312 Nuclease I enzymes are responsible for the degradation of RNA and single-stranded DNA during several plant... - dopsis, and demonstrate that it is a senescence-associated nuclease I gene. BFN1 nuclease shows high

  

Source: Green, Pamela - Delaware Biotechnology Institute & Department of Plant and Soil Sciences, University of Delaware

 

Collection: Biotechnology ; Biology and Medicine

 
4 Copyright 2009 by the Genetics Society of America DOI: 10.1534/genetics.109.108472
 

Summary:  of a lig4 mutant background for piggyBac or Minos excision screens. Zinc-finger nucleases (ZFNs) have... of the Bloom Syndrome DNA Helicase Extends the Utility of Imprecise Transposon Excision for Making Null... for imprecise excision screens to delete genes of interest. However, these screens are highly ...

  

Source: McVey, Mitch - Department of Biology, Tufts University

 

Collection: Biology and Medicine

 
5 Plant Science, 81 (1992)93-102 93 ElsevierScientificPublishers Ireland Ltd.
 

Summary:  Bgeneproductsduring riceplastiddevelopmentis light-regulatedprimarily at translational or post-translational level. Sl-nuclease mapping... . A heterologous in vitro transcription assaywith spinach transcriptionally active high salt extract, followedby Sl-nuclease... was characterized by nuclease S1 mapping and primer extension. ...

  

Source: Chung, Kuang-Ren - Citrus Research and Education Center, University of Florida

 

Collection: Biology and Medicine

 
6 Supplemental Data Passenger-Strand Cleavage Facilitates Assembly
 

Summary:  in water. Sample recovery was about 50%. Diastereomer identity was established by nuclease P1 treatment... . Samples were treated with 2 units nuclease P1 (US Biological) for 60 min at 37°C, dephosphorylated... -30% Buffer B over 35 min. The characteristic nuclease P1-resistant Rp CU dinucleotide eluted from the column

  

Source: Bartel, David - Whitehead Institute for Biomedical Research & Department of Biology, Massachusetts Institute of Technology (MIT)

 

Collection: Biotechnology ; Biology and Medicine

 
7 Review Article Strategies for DNA Interstrand Crosslink Repair
 

Summary:  , it appears that this organism has evolved an excision nuclease-based mecha- nism of repairing ICLs... ; homologous recombination; nucleotide excision repair; translesion synthesis INTRODUCTION DNA interstrand... pathways, including nucleo- tide excision repair (NER) and homologous recombination (HR). ...

  

Source: McVey, Mitch - Department of Biology, Tufts University

 

Collection: Biology and Medicine

 
8 Summary and general discussion Even though DNA has the very important task of storing all genetic information its
 

Summary:  . Examples of these are base excision repair (BER), UV damage endonuclease (UVDE) repair and nucleotide... excision repair (NER). Base excision repair requires the presence of a relatively large number of proteins... B and UvrC. The molecular mechanism of the UvrABC system has been extensively studied and is ...

  

Source: van den Brink, Jeroen - Leiden Institute of Physics, Universiteit Leiden

 

Collection: Physics

 
9 Mutation Research 485 (2001) 2336 Nucleotide excision repair "a legacy of creativity"
 

Summary:  of XPA/RPA to which the excision nucleases XPG and XPF/ERCC1 bind and carry out the 3 and 5 cleavages... systems, e.g. XPG-nth, an association between the NER 3 nuclease with the glycosylase for excision... Mutation Research 485 (2001) 23­36 Nucleotide excision repair "a legacy of ...

  

Source: Karplus, Kevin - Department of Biomolecular Engineering, University of California at Santa Cruz

 

Collection: Biotechnology ; Chemistry

 
10 THESIS APPROVAL The abstract and thesis of Katherine Ona for the Master of Science in Biology were
 

Summary:  . Biochimie 64:595-598. 41. Sancar, A., and W. D. Rupp. 1983. A novel repair enzyme: UVRABC excision nuclease... and recovery following #12;2 treatment with nitrofurazone requires recA, nucleotide excision repair, the rec... excision repair pathways are important for cell survival in the presence of ...

  

Source: Courcelle, Justin - Department of Biology, Portland State University

 

Collection: Biology and Medicine

 
11 Encyclopaedia of Life Science--Editor Stylesheet Copyright Macmillan Reference Ltd 20 November 1998 Page 1
 

Summary:  -repair nuclease subunit A; part of the UvrABC endonuclease that initiates excision repair uvrB 17.5 Helicase... and ATPase; excision-repair nuclease subunit B; part of the UvrABC endonuclease that initiates excision... repair uvrC 43.0 ...

  

Source: Kowalczykowski, Stephen C. - Section of Microbiology, University of California, Davis

 

Collection: Biology and Medicine

 
12 RecA Protein Piero R Bianco,University of California, Davis, California, USA
 

Summary:  with UmuD and Umu(D')2 uvrA 91.9 Excision-repair nuclease subunit A; part of the UvrABC endonuclease... that initiates excision repair uvrB 17.5 Helicase and ATPase; excision-repair nuclease subunit B; part of the UvrABC... endonu- clease that ...

  

Source: Kowalczykowski, Stephen C. - Section of Microbiology, University of California, Davis

 

Collection: Biology and Medicine

 
13 Abasic Site Repair in Higher Eukaryotes Phyllis R. Strauss and Noreen E. O'Regan
 

Summary:  Base-excision repair (BER) refers to a repair pathway that generates and repairs aba- sic sites... polymerase (pol ) fills the gap with the correct nucleotide (reaction 3) and then excises the dRP from... of PCNA causes accumulation of pre-excision reaction intermediates following strand displacement, which

  

Source: Strauss, Phyllis R. - Department of Biology, Northeastern University

 

Collection: Biology and Medicine

 
14 RADIATION RESEARCH 168, 499506 (2007) 0033-7587/07 $15.00
 

Summary:  , A novel repair enzyme: UVRABC excision nuclease of Escherichia coli cuts a DNA strand on both sides... complete degradation of the genomic DNA occurs. Although the RecBCD helicase/nuclease complex is known... -dependent helicase and nuclease that is capable of un- winding and degrading duplex DNA from a ...

  

Source: Courcelle, Justin - Department of Biology, Portland State University

 

Collection: Biology and Medicine

 
15 The obesity-associated Fto gene is a transcriptional coactivator Qiong Wu a,1
 

Summary:  ,1 , Maria Szkudlarek-Mikho a , Ivana de la Serna b , Khew-Voon Chin a,b,c, a Department of Medicine... /EBPs) from unmethylated as well as methylation-inhibited gene promoters. Fto also exhibits nuclease activity... and base excision re- pair via the excision of methylated base by DNA glycosylases [33], or ...

  

Source: Toledo, University of - Center for Diabetes and Endocrine Research (CeDER)

 

Collection: Biology and Medicine

 
16 Purification and Characterization of the DNA Polymerase R Associated Exonuclease: The RTH1 Gene Product
 

Summary:  of RTH1 nuclease revealed that most likely the enzyme can excise an A or T much faster than a G or C. One... is also known as yeast RAD2 homolog 1 or RTH1 nuclease. The native exonuclease also had strong flap... endonuclease activity similar to that observed with RTH1 nuclease and homologous yeast (RAD2) and ...

  

Source: McQuade, D. Tyler - Department of Chemistry and Biochemistry, Florida State University

 

Collection: Chemistry

 
17 Ctp1 and Exonuclease 1, alternative nucleases regulated by the MRN complex, are required
 

Summary:  10 nuclease, required for nucleotide excision- repair, is required for efficient recombination... Ctp1 and Exonuclease 1, alternative nucleases regulated by the MRN complex, are required... homologous DNA, which can produce genetic recombinants. To determine the role of nucleases in DSB end

  

Source: Smith, Gerry - Basic Sciences Division, Fred Hutchinson Cancer Research Center

 

Collection: Biology and Medicine

 
18 Homingendonucleasestructureandfunction Barry L. Stoddard
 

Summary:  , as well as inteins (intervening sequences that are spliced and excised post... a common ancestral nuclease. While crystal structures of several representatives of the LAGLIDADG... in proper splicing and excision of the RNA intron, forming a stable RNP in the process. The spliced intron

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
19 Selective Excision of the Centromere Chromatin Complex from Saccharomycescerevisiae
 

Summary:  Selective Excision of the Centromere Chromatin Complex from Saccharomycescerevisiae Margaret... a CEN fragment that can be selec- tively excised from the chromatin DNA with restric- tion endonucleases... of centromere homology is resistant to nuclease digestion. Restriction enzyme linkers encod- ing the Bam HI

  

Source: Bloom, Kerry S.- Department of Biology, University of North Carolina at Chapel Hill

 

Collection: Biology and Medicine

 
20 Holliday junctions in the eukaryotic nucleus: resolution in sight?
 

Summary:  ,9]. Integrase and nuclease superfamilies of HJ resolvases: the RuvABC model and archaeal Hjc Homologous... action of RuvABC is well-supported by genetic evidence, and mutations in all three genes result... activi- ties suggest that the RuvABC mechanism might be conserved in eukaryotes [15

  

Source: Heyer, Wolf-Dietrich - Sections of Microbiology & Molecular and Cellular Biology, University of California, Davis

 

Collection: Biology and Medicine


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21 THE ROLE OF RECOMBINATION PROTEINS IN MAINTAINING AND PROCESSING ARRESTED REPLICATION FORKS DURING THE
 

Summary:  by the RecJ/RecQ, nuclease/helicase.......................................................34 Figure 1... I, nucleotide excision repair and branch migration by RecG or RuvAB are not required to generate... by the RecJ/RecQ, nuclease/helicase............................................................40 1.6 Model

  

Source: Courcelle, Justin - Department of Biology, Portland State University

 

Collection: Biology and Medicine

 
22 Molecular Cell, Vol. 15, 3141, July 2, 2004, Copyright 2004 by Cell Press A Defined Human System that Supports
 

Summary:  that Supports Bidirectional Mismatch-Provoked Excision prised of DNA helicase II and several single... break that directs repair may reside 3 or 5 to the mispair,Summary and excision removes that portion... of the incised strand spanning the nick and the mismatch (Fang and Modrich,Mismatch-provoked excision directed

  

Source: Burgers, Peter M. - Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis

 

Collection: Biology and Medicine

 
23 Spectroscopic and Theoretical Insights into Sequence Effects of Aminofluorene-Induced Conformational Heterogeneity and Nucleotide Excision
 

Summary:  . Nucleotide Excision Repair Assays NER assays were conducted using the E. coli UvrABC nuclease system. DNA... of Escherichia coli UvrABC-based nucleotide excision repair in a conformation-specific manner: i.e., greater... -Induced Conformational Heterogeneity and Nucleotide ...

  

Source: Cho, Bongsup P. - Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island

 

Collection: Biology and Medicine

 
24 Annu. Rev. Biochem. 2005. 74:681710 doi: 10.1146/annurev.biochem.74.082803.133243
 

Summary:  by which the newly replicated strand is identified, and on excision of the replication error. CONTENTS... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 697 Strand Excision... GATC sites None - Complex Loads -clamp onto DNA RFC complex Loads PCNA, modulates excision polarity

  

Source: Erie, Dorothy A. - Department of Chemistry, University of North Carolina at Chapel Hill

 

Collection: Chemistry ; Biology and Medicine

 
25 RecA Protein Piero R Bianco, University of California, Davis, California, USA
 

Summary:  of the UvrABC endonuclease that initiates excision repair uvrB Helicase and ATPase; excision-repair nuclease... subunit B; part of the UvrABC endonuclease that initiates excision repair uvrC Excision-repair nuclease... with ...

  

Source: Kowalczykowski, Stephen C. - Section of Microbiology, University of California, Davis

 

Collection: Biology and Medicine

 
26 A phosphoramidate substrate analog is a competitive inhibitor of the Tetrahymena group I ribozyme
 

Summary:  with natural nucleic acids, including triple helices [27], and they show signi¢cant nuclease resistance [28... a cyclic phosphate intermediate, recog- nizes and cleaves the r-NP backbone. Micrococcal nuclease (S7... to cleave both d-NP and r-NP. However, nucleases P1 and S1, which both target the O3P^P bond, are not able

  

Source: Doudna, Jennifer A. - Department of Molecular and Cell Biology, University of California at Berkeley

 

Collection: Biology and Medicine

 
27 An Alternative Form of Replication Protein A Expressed in Normal Human Tissues Supports DNA Repair*
 

Summary:  , the nucleotide excision repair activity (excision nuclease) has been reconsti- tuted in vitro with purified... or stabilizing the proper assembly of the excision nuclease. Formation of these complexes may be promoted... that undertheseconditions,therateofexcisionbyaRPA-reconstituted ...

  

Source: Borgstahl, Gloria - Eppley Institute for Cancer Research, University of Nebraska Medical Center

 

Collection: Biology and Medicine

 
28 JOURNAL OF VIROLOGY, Oct. 1984, p. 255-265 0022-538X/84/100255-11$02.00/0
 

Summary:  were eluted from low-melting-point agarose (Bio-Rad Laboratories) gels by excising the DNA fragment... with S1 nuclease. The procedure of Berk and Sharp (7) was applied. The DNA used for hybridization... of denatured calf thymus DNA-100 U of Si nuclease (Sigma) was added. The mixture was incubated at 20°C for 1 h

  

Source: Lübbert, Hermann - Fakultät für Biologie, Ruhr-Universität Bochum

 

Collection: Biology and Medicine

 
29 Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases
 

Summary:  Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases Kelly... injection of mRNAs encoding specific zinc-finger nucleases (ZFNs). Both local mutagenesis via nonhomologous... experimental organisms. Zinc-finger nucleases (ZFNs) are proving to be powerful tools for directed genome

  

Source: NONE; Rutter, Jared - Department of Biochemistry, University of Utah

 

Collection: Biology and Medicine

 
30 Holliday junction-binding peptides inhibit distinct junction-processing enzymes
 

Summary:  of Escherichia coli and interfere with the resolution of HJ substrates by the RuvABC complex. Our results suggest... and in vivo. homologous recombination recombination-dependent repair RecG helicase RuvABC resolvasome tyrosine... -planar HJ and inhibit the RecG helicase and RuvABC resolvase of Escherichia coli. Materials and ...

  

Source: Segall, Anca - Department of Biology, San Diego State University

 

Collection: Biology and Medicine

 
31 Nucleic Acids 3rd period (10:10 am-11:00 am)
 

Summary:  Detection McEachern September 15 Alteration of cloned DNA: Nucleases and site-directed mutagenesis Mc... reactions involving RNA: Nucleases Kushner September 22 Enzymatic reactions involving RNA: poly... Eachern #12;2 October 27 DNA repair: excision repair Kushner October 29 Fall Break November 1 DNA repair

  

Source: Arnold, Jonathan - Nanoscale Science and Engineering Center & Department of Genetics, University of Georgia

 

Collection: Biotechnology

 
32 Annu. Rev. Genet. 2003. 37:61146 doi: 10.1146/annurev.genet.37.110801.142616
 

Summary:  BCD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 630 RuvABC... -strand interruptions due to excision-repair events in progress. These biologically important lesions im- pose... bases and remove them to initiate base excision repair (64, 177). Bulky base ...

  

Source: Courcelle, Justin - Department of Biology, Portland State University

 

Collection: Biology and Medicine

 
33 Copyright 1998 by the Genetics Society of America Damage Control: The Pleiotropy of DNA Repair Genes in Drosophila melanogaster
 

Summary:  characteristics and their intide adduct on an otherwise normal duplex, which can be excised by the UvrABC... of unrepaired lesionsreconstituted human excision nuclease, cleave adjacent to interstrand crosslinks... transposase excises an ele- ment from a donor site, leaving a DSB (Figure ...

  

Source: Sekelsky, Jeff - Department of Biology, University of North Carolina at Chapel Hill

 

Collection: Biology and Medicine

 
34 MOLECULAR PROCESSING OF REPLICATION INTERMEDIATES IN ESCHERICHIA COLI AFTER DNA DAMAGE
 

Summary:  then be degraded by the RecBCD helicase-nuclease or collapse to from a double strand break if RuvABC resolves... ............................................................................................... 90 A.2 Nucleotide excision repair, but not translesion DNA synthesis, is required for the recovery... ...

  

Source: Courcelle, Justin - Department of Biology, Portland State University

 

Collection: Biology and Medicine

 
35 Activities of human exonuclease 1 that promote cleavage of transcribed immunoglobulin
 

Summary:  cleavage. Excision is nick-dependent and structure-dependent. These results lead to a model for exonuclease... to class III of the RAD2 family of nucleases, which includes structure-specific nucleases like flap... , dependent on either uracil excision by UNG or mismatch recognition by MutS (17). DNA nicking ...

  

Source: Maizels, Nancy - Department of Biochemistry, University of Washington at Seattle

 

Collection: Biology and Medicine

 
36 ENZYMATIC PROCESSING OF REPLICATION INTERMEDIATES THAT OCCUR FOLLOWING UV-INDUCED DNA DAMAGE IN ESCHERICHIA COLI
 

Summary:  nucleotide excision repair. Proc Natl Acad Sci U S A 99, 1467-72. Orren, D. K. & Sancar, A. (1989). The (A)BC... that RecG and RuvABC can catalyze this reaction on synthetic DNA substrates and it has been proposed... , indicating that RuvABC and RecG are not essential for the resumption of replication. ...

  

Source: Courcelle, Justin - Department of Biology, Portland State University

 

Collection: Biology and Medicine

 
37 Creating Directed Double-strand Breaks with the Ref Protein A NOVEL RecA-DEPENDENT NUCLEASE FROM BACTERIOPHAGE P1*S
 

Summary:  Creating Directed Double-strand Breaks with the Ref Protein A NOVEL RecA-DEPENDENT NUCLEASE FROM... A-dependent nuclease. Ref binds to ss- and dsDNA but does not cleave any DNA substrate until RecA protein and ATP... as a co-nuclease in the Ref/RecA system. Ref nuclease activity can be limited to the targeted ...

  

Source: Cox, Michael M. - Department of Biochemistry, University of Wisconsin at Madison

 

Collection: Biology and Medicine

 
38 JOURNAL OF BACTERIOLOGY, Mar. 1996, p. 13471350 Vol. 178, No. 5 0021-9193/96/$04.00 0
 

Summary:  more readily than a CPD, which may indicate a greater affinity of the excision repair apparatus for (6... following irradiation. This may be a result of a greater affinity of the Uvr(A)BC nucleotide excision repair... - nuclease V to incise bacterial DNA specifically at the sites of CPDs and found about 80% repair in 20 min

  

Source: Courcelle, Justin - Department of Biology, Portland State University

 

Collection: Biology and Medicine

 
39 Molecular Cell 23, 133142, July 7, 2006 2006 Elsevier Inc. DOI 10.1016/j.molcel.2006.06.005 The Exonuclease TREX1 Is in the SET Complex
 

Summary:  A-treated cells as well as a specific inhibitor of the nuclease. The GzmA-activated endonuclease is NM23- H1... 1 endo- nuclease is activated. The importance of NM23-H1 and SET in GzmA-mediated cell death... endonuclease re- sponsible for base excision repair, Ape1 (Fan et al., 2003b), and the DNA-binding protein HMG

  

Source: Lieberman, Judy - Immune Disease Institute, Harvard University

 

Collection: Biology and Medicine

 
40 JOURNAL OF BACTERIOLOGY, Feb. 2010, p. 665673 Vol. 192, No. 3 0021-9193/10/$12.00 doi:10.1128/JB.01018-09
 

Summary:  Characterization of the Nucleotide Excision Repair System of Neisseria gonorrhoeae Brian E. LeCuyer, Alison K... excision repair (NER) is universally used to recognize and remove many types of DNA damage. In eubacteria... , nucleotide excision repair (NER), recombination repair, and replication restart (10, 18). Among the ...

  

Source: Seifert, Hank - Department of Microbiology-Immunology, Northwestern University

 

Collection: Biology and Medicine


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41 Supporting Information DNA-Templated Polymerization of Side-Chain-Functionalized Peptide Nucleic Acid
 

Summary:  (for lane 13 in Figure 3a, the fastest migrating band was excised; the major bands were excised... for the other samples) were visualized by UV shadowing and excised. The excised polyacrylamide was homogenized... in 19 µL 1x S1 nuclease reaction buffer (Promega) and 1 µL S1 ...

  

Source: Liu, David R. - Department of Chemistry and Chemical Biology, Harvard University

 

Collection: Chemistry ; Biology and Medicine

 
42 . The EMBO Journal vol.14 no.1 pp.17&-187, 1995 -S ^ In vitro RNA editing-like activity in a mitochondrial
 

Summary:  was obtained. Inliibition by micrococcal nuclease suggests a requirement for some type of endogenous RNA... within the 5' RNase H fragment. Nuclease PI digestion of the 5' fragment of pNB2 RNA labeled with [a... and the oligomer S165. The S' fragment was gel-isolated, digested with IWase T2 (A) or nuclease PI (B

  

Source: Simpson, Larry - Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles

 

Collection: Biology and Medicine

 
43 Principles that Govern the Folding of Protein Chains Author(s): Christian B. Anfinsen
 

Summary:  received from the SwedishRoyalAcademyof Sciences specifically cites "... studies on ribo- nuclease... staphylococcal nuclease .The origi- molecule, but I will first summarize this conclu- some of the older... , by excision of two internal bits of se- quence. The elegant studies of Steiner and his

  

Source: Mittal, Aditya - School of Biological Sciences, Indian Institute of Technology, Delhi

 

Collection: Biotechnology ; Biology and Medicine

 
44 MOLECULAR AND CELLULAR BIOLOGY, Dec. 2003, p. 85058518 Vol. 23, No. 23 0270-7306/03/$08.00 0 DOI: 10.1128/MCB.23.23.85058518.2003
 

Summary:  -PK) regulates the DNA damage introduced during Sleeping Beauty (SB) element excision and reinsertion... excision were efficiently and accurately processed by the major Ku-dependent nonhomologous... transposable elements were not found to be efficiently circularized after transposase-mediated excision, DNA

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
45 Copyright 2002 by the Genetics Society of America Efficient Mobilization of mariner in Vivo Requires Multiple Internal Sequences
 

Summary:  ABSTRACT Aberrant products of mariner excision that have an impaired ability to be mobilized often include... binding sites. The isolation of excision-defective elements with point mutations at nucleotide positions... of type II transposons (DNA transpo- nuclease degradation (Staveley et al. 1995), therebysons

  

Source: Hartl, Daniel L. - Department of Organismic and Evolutionary Biology, Harvard University

 

Collection: Environmental Sciences and Ecology ; Biology and Medicine

 
46 This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research
 

Summary:  potential biotechnologies, such as zinc finger nucleases, that could be also used for transgene excision... 's personal copy Keeping the genie in the bottle: transgene biocontainment by excision in pollen Hong S. Moon1... . Site- specific recombination systems could allow highly effi- cient excision of ...

  

Source: Dai, Pengcheng - Department of Physics and Astronomy, University of Tennessee

 

Collection: Materials Science ; Physics

 
47 A human nuclease specific for G4 DNA , Akiko Yabuki*
 

Summary:  A human nuclease specific for G4 DNA Hui Sun* , Akiko Yabuki* , and Nancy Maizels*§¶ Departments... 9, 2001) We have identified a human nuclease that specifically cleaves four-stranded DNA stabilized... by G quartets (G4 DNA). This nucle- ase, GQN1 (G quartet nuclease 1), cuts within the single

  

Source: Maizels, Nancy - Department of Biochemistry, University of Washington at Seattle

 

Collection: Biology and Medicine

 
48 THE RECOVERY OF DNA REPLICATION IN ESCHERICHIA COLI IRRADIATED WITH ULTRAVIOLET LIGHT
 

Summary:  Chapter 3.Recovery of DNA replication in UV-irradiated Escherichia coli requires both excision repair... ArecA double mutant (figure 2A). Since uvrA mutants are unable to excise UV-induced lesions, they hypothesized... of the uvrA and uvrB genes for excision repair and the umuCD genes for bypassing replication blocking DNA

  

Source: Courcelle, Justin - Department of Biology, Portland State University

 

Collection: Biology and Medicine

 
49 Molecular Microbiology (2001) 40(1), 257269 BldD is a direct regulator of key developmental genes in
 

Summary:  of mutation in bldD on the expression of these three target genes was assessed using S1 nuclease protection... al., 1987), an ATP-binding cassette (ABC) membrane-spanning transporter (bldK; Nodwell et al., 1996... implicated in signal uptake. bldK is a complex locus that encodes an ABC transporter, and bldK mutants

  

Source: Elliot, Marie A. - Department of Biology, McMaster University

 

Collection: Biology and Medicine

 
50 Interstrand crosslink repair: can XPF-ERCC1 be let off the hook?
 

Summary:  replication protein A positions nucleases in nucleotide excision repair. Genes Dev. 12, 2598­2609 27 Thompson... that the endonuclease xeroderma pigmentosum complementation group F-excision repair cross-comple- menting rodent repair... deficiency complementation group 1 (XPF-ERCC1) has been heretofore misplaced. During ...

  

Source: Sekelsky, Jeff - Department of Biology, University of North Carolina at Chapel Hill

 

Collection: Biology and Medicine

 
51 Vol. 10, No. 11 Nucleosome Depletion Alters the Chromatin Structure of
 

Summary:  nuclease-resistant chromatin core of approximately 200 base pairs of DNA. The structure of the centromere... exhibit altered chromatin structures in vivo. Thus the structure, as delineated by nuclease digestion... the cell incapable of chromosome segregation. Histone repression resulted in increased nuclease sensitivity

  

Source: Bloom, Kerry S.- Department of Biology, University of North Carolina at Chapel Hill

 

Collection: Biology and Medicine

 
52 Domain Mapping of Human Apurinic/Apyrimidinic Endonuclease STRUCTURAL AND FUNCTIONAL EVIDENCE FOR A DISORDERED AMINO TERMINUS AND A TIGHT
 

Summary:  the nuclease fold with restoration of globular conformation for the carboxyl two-thirds of the molecule. Of all... of the nuclease fold, there must be multiple means by which DNA containing an abasic site associates... similarity, but also the tertiary structure of the putative active site (the nuclease fold) is conserved (5

  

Source: Strauss, Phyllis R. - Department of Biology, Northeastern University

 

Collection: Biology and Medicine

 
53 JOURNAL OF BACTERIOLOGY, Aug. 2007, p. 54525462 Vol. 189, No. 15 0021-9193/07/$08.00 0 doi:10.1128/JB.00408-07
 

Summary:  be repaired by nucleotide excision repair or bypassed by translesion DNA polymerases (7, 9, 10, 13, 15... , and are significantly reduced in ruvABC mutants, which lack an enzyme complex that catalyzes branch migration... that following replication arrest in dnaB(Ts) mutants, RuvABC catalyzes the displacement and regression

  

Source: Courcelle, Justin - Department of Biology, Portland State University

 

Collection: Biology and Medicine

 
54 Distinct Roles of Two Mg2+ Binding Sites in Regulation of Murine Flap
 

Summary:  ) and MutH (36) nucleases, which are involved in many DNA repair pathways, including nucleotide excision... + or Mn2+, are required for the active center of FEN-1 nucleases. However, it remains unclear as to how Mg... nuclease activities in a mode different from the "two-metal mechanism". Murine flap ...

  

Source: Krishnamoorthi, Ramaswamy - Department of Biochemistry, Kansas State University

 

Collection: Biology and Medicine

 
55 DNA Repair 8 (2009) 803812 Contents lists available at ScienceDirect
 

Summary:  of the FHA domain. 3.3. Synthesis-dependent strand annealing in nbs mutants We used a P element excision... instead of red eyes. Excision of the P{wa} element was carried out in males by crossing in P transposase... . Excision generates a 14-kb gap relative to the sister chromatid, which is the only template for HR repair

  

Source: Sekelsky, Jeff - Department of Biology, University of North Carolina at Chapel Hill

 

Collection: Biology and Medicine

 
56 www.landesbioscience.com Fly 1 Fly 4:2, 1-4;April/May/June 2010; 2010 Landes Bioscience
 

Summary:  excision for making null mutations in Drosophila. Genetics 2009; 183:1187­93; PMID: 19687136; DOI: 10... are frequently utilized in imprecise excision screens to generate deletions in genes of interest. In general... restored P elements, internally deleted P elements, precise excisions, or repair events that delete DNA

  

Source: McVey, Mitch - Department of Biology, Tufts University

 

Collection: Biology and Medicine

 
57 2000 Macmillan Magazines Ltd NATURE CELL BIOLOGY | VOL 2 | JULY 2000 | www.nature.com/ncb 399
 

Summary:  .nature.com/ncb 399 ABC1 promotes engulfment of apoptotic cells and transbilayer redistribution of phosphatidylserine... -binding-cassette transporter 1 (ABC1) has been implicated in processes related to membrane-lipid turnover. Here, using in vivo... loss-of-function and in vitro gain-of-function models, we show that ABC1 ...

  

Source: Institut de Biologie Physico-Chimique CNRS, Physico-Chimie Moléculaire des Membranes Biologiques

 

Collection: Chemistry ; Biology and Medicine

 
58 Distinct Activities of Exonuclease 1 and Flap Endonuclease 1 at Telomeric G4 DNA
 

Summary:  endonuclease 1 (FEN1) are members of the RAD2 family of structure-specific nucleases. Genetic analysis has... Exonuclease 1 (EXO1) and Flap endonuclease 1 (FEN1) belong to the RAD2 family of structure-specific nucleases... . They share a core nuclease domain that is remarkably conserved from yeast to mammals [1], and display both ...

  

Source: Maizels, Nancy - Department of Biochemistry, University of Washington at Seattle

 

Collection: Biology and Medicine

 
59 Samenvatting en algemene discussie Het DNA, de drager van alle genetische informatie, wordt constant bedreigd
 

Summary:  laatstgenoemde klassen van herstelmechanismen zijn base-excision herstel (BER), UV damage endonuclease (UVDE... herkennen en het DNA te knippen: UvrA, UvrB en UvrC. Het moleculaire mechanisme van het UvrABC systeem... C is de nuclease van het systeem en knipt aan weerszijden van de schade zodra de aanwezigheid van de

  

Source: van den Brink, Jeroen - Leiden Institute of Physics, Universiteit Leiden

 

Collection: Physics

 
60 Cell, Vol. 90, 10311039, September 19, 1997, Copyright 1997 by Cell Press The Transmembrane Kinase Ire1p Is a
 

Summary:  activation of an unknown nuclease. In the absence of the second step in the splicing reaction (ligation... RNA, to a constitutively active endo- after binding to the unfolded protein response element nuclease (such as t... Has Both Kinase and Nuclease Activity In Vitro (A) Amino-acid sequence alignment of the C

  

Source: Walter, Peter - Department of Biochemistry and Biophysics, University of California at San Francisco

 

Collection: Biology and Medicine


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61 Copyright 2007 by the Genetics Society of America DOI: 10.1534/genetics.106.070052
 

Summary:  ), and defects in repair of DSBs generated by excision of transposable elements (Boyd et al. 1981; Beall and Rio... generated by P-element excision (reviewed in Adams and Sekelsky 2002). P{EPgy2}mus309EY03745 harbors a P... of mus309 mutations in these homozygotes (data not shown). A total of 759 excisions were screened by PCR

  

Source: McVey, Mitch - Department of Biology, Tufts University; Sekelsky, Jeff - Department of Biology, University of North Carolina at Chapel Hill

 

Collection: Biology and Medicine

 
62 Sequence effects of aminofluorene-modified DNA duplexes: thermodynamic and circular
 

Summary:  are elimi- nated by NER (3,12,17). The AF-lesion excision efficiency by the E.coli UvrABC system is known... and incision by UvrABC nuclease. Biochemistry, 42, 12654­12661. 12. Singer,B. and Hang,B. (2000) Nucleic acid... ,L.J. (1998) Rate of incision of N-acetyl-2-aminofluorene and N-2-aminofluorene adducts ...

  

Source: Cho, Bongsup P. - Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island

 

Collection: Biology and Medicine

 
63 J. Mol.Biol. (1985) 185,625-637 Bacterial DNA Topoisomerase I Can Relax Positively
 

Summary:  section (see the legend to Fig. 4). Restriction sites used in excising and labeling fragments are Bam... reaction mixtures. Staphylococcal nuclease footprints were performed in the same way, except that a 5 mg... /ml Staphylococcal nuclease stock solution was diluted to 25 pg/ml before the addition of 1 gl to the reaction

  

Source: Kirkegaard, Karla - Department of Microbiology and Immunology, Stanford University

 

Collection: Biology and Medicine

 
64 nature structural biology volume 5 number 8 august 1998 707 1Structural Biology Center, Korea Institute of Science & Technology, P.O. Box 13, Cheongryang, Seoul 130-650, South Korea. 2Both authors contributed equally to the work.
 

Summary:  , also called RAD27/RTH1/MF-1/DNase IV), a structure specific nuclease with 5' flap DNA endonuclease... , proliferating cell nuclear antigen physically interacts with FEN-1 and stimu- lates its nuclease activity by 10... endonuclease-1 (FEN-1), a structure specific nuclease, is an essential enzyme for eukaryotic DNA replication

  

Source: Cho, Yunje - Department of Life Science, Pohang University of Science and Technology

 

Collection: Biology and Medicine

 
65 RuvABC Is Required to Resolve Holliday Junctions That Accumulate following Replication on Damaged Templates
 

Summary:  RuvABC Is Required to Resolve Holliday Junctions That Accumulate following Replication on Damaged... 97207 RuvABC is a complex that promotes branch migration and resolution of Holliday junctions. Although... ruv mutants are hypersensitive to UV irradiation, the molecular event(s) that necessitate RuvABC

  

Source: Courcelle, Justin - Department of Biology, Portland State University

 

Collection: Biology and Medicine

 
66 Molecular Cell, Vol. 15, 607620, August 27, 2004, Copyright 2004 by Cell Press The Fanconi Anaemia Gene FANCC Promotes
 

Summary:  DNA damage. Here, we use the model vertebrate cell excision repair nucleases ERCC1 and XPF results... substrate of after the initial excision step. Studies in budding yeast point to a similar situation. Mutants... in the Rad3 (deficientthis nuclear complex is the downstream FA protein in nucleotide excision ...

  

Source: Patel, KJ - Laboratory of Molecular Biology, MRC

 

Collection: Biology and Medicine

 
67 Comparative Biochemistry and Physiology Part C 136 (2003) 343356 1532-0456/03/$ -see front matter 2003 Elsevier Inc. All rights reserved.
 

Summary:  al., 1995a,b,c), and the toxicity of the recom- binant enzyme has been extended to oocyte mat... , respectively, of a ribonuclease. Both testes with their epididymides and seminal vesicles were excised after 20... with collagen fibres, fibroblasts, and fibrocytes was included in the histological assessment of excised testes

  

Source: Raines, Ronald T. - Departments of Biochemistry & Chemistry, University of Wisconsin at Madison

 

Collection: Chemistry ; Biology and Medicine

 
68 ITCHY Libraries 129 From: Methods in Molecular Biology, vol. 231: Directed Evolution Library Creation: Methods and Protocols
 

Summary:  are prepared by treatment with a single-strand nuclease and a DNA polymerase. This synchronized truncation... - rated S-dNMP (6). Blunt ends are prepared by treatment with a single-strand nuclease and a DNA... .6 mM MgCl2. 7. Mung Bean Nuclease. 8. 10X Mung bean buffer: 500 mM sodium acetate, pH 5.0, 300 mM Na

  

Source: Gray, Jeffrey J. - Department of Biomolecular and Chemical Engineering, Johns Hopkins University; Ostermeier, Marc - Department of Biomolecular and Chemical Engineering, Johns Hopkins University

 

Collection: Biology and Medicine ; Biotechnology ; Chemistry

 
69 The EMBO Journal vol.7 no. 1 1 pp.3559 -3569, 1988 Ribosome pausing and stacking during translation of a
 

Summary:  . Translating extracts are treated with micrococcal nuclease at a concentration sufficient to trim away portions... .Walter nuclease Iextract mRNA fragments fragments (steps D and E). Because this polymerase, in the absence... are treated with nuclease to trim away fragments of RNA that are not associated with ribosomes (A

  

Source: Walter, Peter - Department of Biochemistry and Biophysics, University of California at San Francisco

 

Collection: Biology and Medicine

 
70 Cytogenet Genome Res 107:172179 (2004) DOI: 10.1159/000080595
 

Summary:  com- plex of human DNA repair excision nuclease. J Biol Chem 230:22657­22660 (1995). Riechmann V... -mail: sekelsky@unc.edu ABC Fax + 41 61 306 12 34 E-mail karger@karger.ch www.karger.com © 2004 S. Karger AG... -9 is the Drosophila ortholog of the nucleotide excision repair (NER) endonuclease XPF (human

  

Source: Sekelsky, Jeff - Department of Biology, University of North Carolina at Chapel Hill

 

Collection: Biology and Medicine

 
71 New Pathways in Folding of the Tetrahymena Group I Rick Russell and Daniel Herschlag*
 

Summary:  of the periph- eral element P5abc. This, along with other results, has suggested that P5abc may serve... cleavage activity as a readout for native state formation and investigate the effect of P5abc on the rate... of folding to the native structure. Despite the early folding of P5abc, its removal to give the ...

  

Source: Herschlag, Dan - Department of Biochemistry, Stanford University

 

Collection: Biology and Medicine

 
72 www.landesbioscience.com Cell Cycle 221 [Cell Cycle 4:2, 221-224; February 2005]; 2005 Landes Bioscience
 

Summary:  polymerase, Okazaki fragment, idling, nuclease, nick translation, FEN1, PCNA ABBREVIATIONS Pol , DNA... metabolic processes, such as base excision repair, that depend on Pol for synthesis. INTRODUCTION... maturation where it degrades the initiator RNA.8 However, FEN1 also functions in long patch base-excision

  

Source: Burgers, Peter M. - Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis

 

Collection: Biology and Medicine

 
73 Genome sequence of Halobacterium species NRC-1 Wailap Victor Nga,b
 

Summary:  appear to be complete as copies of the uvrABC nuclease and uvrD helicase, and endonucleases... . Accordingly, NRC-1 has multiple active K transporters, includ- ing KdpABC, an ATP-driven K transport system... described previously (41). Interestingly, Kd- pABC, TrkA (three of five copies), and NhaC (one of ...

  

Source: Lowe, Todd M. - Department of Biomolecular Engineering, University of California at Santa Cruz

 

Collection: Biology and Medicine ; Biotechnology

 
74 JOURNAL OF VIROLOGY, 0022-538X/00/$04.00 0
 

Summary:  repair or base excision repair similarly require a polymerase, a nuclease, and a ligase to repair gaps... , or reverse transcriptase), a nuclease (flap endonuclease), and a ligase (ligase I, III, or IV and its... , a nuclease to remove the 5 flap, and a ligase to join the strands (Fig. 1A). Repair pathways ...

  

Source: Bushman, Frederic - Department of Microbiology, University of Pennsylvania

 

Collection: Biology and Medicine

 
75 Annu. Rev. Genet. 2001. 35:5382 Copyright c 2000 by Annual Reviews. All rights reserved
 

Summary:  of DNA metabolism, and an illuminating one. Replication fork repair can take its place with excision... repair, mismatch repair, base excision re- pair, and direct repair [such as the reaction catalyzed by DNA... of replication forks functions to maintain genome integrity. Most DNA repair processes (such as excision repair

  

Source: Cox, Michael M. - Department of Biochemistry, University of Wisconsin at Madison

 

Collection: Biology and Medicine

 
76 Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection
 

Summary:  Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection Yoko... variant of gene targeting uses direct microinjection of custom-designed Zinc Finger Nuclease (ZFN) m... finger nucleases (ZFNs), which are * Corresponding author. Tel.: þ420 387775283; fax: þ420 385310354. E

  

Source: Žurovec, Michal - Department of Physiology, University of South Bohemia

 

Collection: Biology and Medicine

 
77 Proc. Nati, Acad. Sci. USA Vol. 79, pp. 4030-4034, July 1982
 

Summary:  RNA and S1 Nuclease Analysis. Totalcellular RNA was prepared as described by Favaloro et aL (13) except... the text. Excised segment in pBRdrepresents the deletionthatremoves sequences that inhibit SV40-pBR322... gene. H, Hindm; B, BamHI. S1 nuclease analysis (13, 14) the antisense strand ofa Hae III/ HinfI ,B

  

Source: DiMaio, Daniel - Departments of Genetics & Molecular Biophysics and Biochemistry, Yale University

 

Collection: Biology and Medicine

 
78 Genomic demography: a life-history analysis of transposable element evolution
 

Summary:  1998; Jordan & McDonald 1998, 1999a,b,c; Kim et al. 1998). Knowledge of transposition and excision... ^transposon coevolution. Evolutionary change in Ty elements depends on their replication and excision rates, which have... /2 families, we develop a novel `genomic demography' model to estimate long-term transposition and ...

  

Source: Promislow, Daniel - Department of Genetics, University of Georgia

 

Collection: Biology and Medicine

 
79 Annu. Rev. Genet. 2001. 35:24374 Copyright c 2001 by Annual Reviews. All rights reserved
 

Summary:  , as assayed by the accessibility of micro- coccal nuclease to the DNA in isolated chromatin. This alteration... . The recB1080 (D1080A) mutation, which abolishes the nuclease activities (132), blocks RecA loading (3, 5... ), suggesting that some aspect of the Chi-nuclease interaction must precede RecA loading. In accord with a ...

  

Source: Smith, Gerry - Basic Sciences Division, Fred Hutchinson Cancer Research Center

 

Collection: Biology and Medicine

 
80 Molecular Microbiology (2003) 50(2), 475486 doi:10.1046/j.1365-2958.2003.3728.x 2003 Blackwell Publishing Ltd
 

Summary:  is taken up via the bldK-specified ABC transporter (Nodwell et al., 1996; Nodwell and Losick, 1998... delayed produc- tion of Red (Takano et al., 2001). We analysed adpAc expression by S1 nuclease mapping... eliminating g-butyrolactone production in liquid cultures. A. S1 nuclease mapping of the transcriptional start

  

Source: Bibb, Mervyn - Department of Molecular Microbiology, John Innes Centre; Buttner, Mark - Department of Molecular Microbiology, John Innes Centre

 

Collection: Biology and Medicine


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81 Z .Mutation Research 459 2000 219228 www.elsevier.comrlocaterdnarepair
 

Summary:  and characterization of the XPF­ERCC1 complex of human Z .DNA repair excision nuclease, J. Biol. Chem. 230 1995 22657... .elsevier.comrlocatermutres Nucleotide excision repair endonuclease genes in Drosophila melanogaster Jeff J. Sekelsky a,b,) , Katheryn J... .Nucleotide excision repair NER is the primary ...

  

Source: Sekelsky, Jeff - Department of Biology, University of North Carolina at Chapel Hill

 

Collection: Biology and Medicine

 
82 Stimulation of RTH1 Nuclease of the Yeast Saccharomyces cereVisiae by Replication Protein A
 

Summary:  Stimulation of RTH1 Nuclease of the Yeast Saccharomyces cereVisiae by Replication Protein A Esther... Vember 22, 1996; ReVised Manuscript ReceiVed March 19, 1997X ABSTRACT: The RTH1 nuclease is involved... in the function of RTH1 nuclease, including its structure specific endonuclease activity. Initial in Vitro studies

  

Source: McQuade, D. Tyler - Department of Chemistry and Biochemistry, Florida State University

 

Collection: Chemistry

 
83 Q 1992 by The AmericanSociety for Biochemistry and MolecularBiology, Inc. OFBIOLOGICAL CHEMISTRY Vol. 267, No. 11, Issue of April 15,pp. 7683-7689,1992
 

Summary:  ; SDS, sodium dodecyl sulfate; LB, Luria broth;TB, terrific broth; SN,staphylococ- cal nuclease; PMSF... of the transmembranedomainofGpA. This domain is fusedto the C terminus of staphylococcal nuclease via a flexible linker... nuclease Q149P (pPCV-I) was kindly suppliedby A. Frankel (WistarInstitute).Restriction enzymes, T4 DNA

  

Source: Engelman, Donald M.- Department of Molecular Biophysics and Biochemistry, Yale University

 

Collection: Biology and Medicine

 
84 The role of tryptophan in staphylococcal nuclease stability Hong-Yu Hu a
 

Summary:  The role of tryptophan in staphylococcal nuclease stability Hong-Yu Hu a , Ming-Chya Wu a,b,c... : Staphylococcal nuclease W140A F61W/W140A Y93W/W140A Tryptophan modification Local hydrophobicity Staphylococcal... nuclease (SNase) has a single Trp residue at position 140. Circular dichroism, intrinsic and ANS

  

Source: Academia Sinica (Taiwan), Institute of Physics, Laboratory of Statistical and Computational Physics

 

Collection: Physics

 
85 Supporting Information For: Removal of Covalent Heterogeneity Reveals Simple Folding Behavior for P4-P6 RNA
 

Summary:  standard P1 nuclease reaction conditions involving a 30 minute incubation with 1x P1 (see Experimental... ) and bovine serum albumin (BSA) were purchased from New England BioLabs (MA). P1 Nuclease from Penicillium... TAT A #12;S13 Native Gel Assays and P1 Nuclease Digestions P4-P6 was transcribed from a double

  

Source: Herschlag, Dan - Department of Biochemistry, Stanford University

 

Collection: Biology and Medicine

 
86 Modeling of Flap Endonuclease Interactions with DNA Substrate
 

Summary:  of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037 USA Structure-specific 50 nucleases... -specific 50 nucleases. q 2003 Elsevier Science Ltd. All rights reserved Keywords: structure-specific 50... nuclease; FEN1; point mutation; methylphosphonate; 20 -O-methyl*Corresponding author Introduction Structure

  

Source: Onufriev, Alexey - Departments of Computer Science & Physics, Virginia Tech

 

Collection: Physics ; Biology and Medicine

 
87 RESEARCH ARTICLE Open Access Characterization of a Mycobacterium smegmatis
 

Summary:  sleeves": DNA damage recognition by the UvrABC nuclease system. Mutat Res 2005, 577:92-117. 15. Kurthkoti... of the Nucleotide Excision Repair system (NER). The two mutants showed identical phenotypes, although the respective... excision repair system (NER) and is highly conserved among mycobacteria. NER ...

  

Source: Paris-Sud XI, Université de - Institut d'Optique, Laboratoire Charles-Fabry, Groupe d'Optique Atomique

 

Collection: Physics

 
88 COMMUNICATION Identification of the RecA Protein-loading Domain of
 

Summary:  helicase, and an ATP-depen- dent nuclease. The enzyme initiates the process of homologous recombination... to be productive in recombination, the destructive nuclease activity must be carefully controlled. w exerts its... degradation of DNA by regu- lating the enzyme's asymmetric nuclease activities (Anderson & ...

  

Source: Kowalczykowski, Stephen C. - Section of Microbiology, University of California, Davis

 

Collection: Biology and Medicine

 
89 Xpf and Not the Fanconi Anaemia Proteins or Rev3 Accounts for the Extreme Resistance to Cisplatin in
 

Summary:  excision repair nuclease subcomponent XPF is essential for crosslink repair The fact that Dictyostelium... ). In summary, the excision repair nuclease subcomponent xpf is essential for repairing crosslinks... for crosslink repair all involve a nuclease(s) carrying out the ...

  

Source: Babu, M. Madan - Laboratory of Molecular Biology, MRC; Patel, KJ - Laboratory of Molecular Biology, MRC

 

Collection: Biology and Medicine ; Biotechnology

 
90 DNA Unwinding Step-size of E. coli RecBCD Helicase Determined from Single Turnover Chemical
 

Summary:  DNA exo- nuclease, ssDNA endonuclease, DNA-dependent ATPase, and helicase activities.11,12 Through a com... DNA end. The enzyme shows no detectable nuclease activity on circular dsDNA; potent nuclease activity... helicase involved in methyl-directed mismatch repair29 and nucleotide excision repair,30,31 ...

  

Source: Cistola, David P. - Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis; Lohman, Timothy M. - Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis; Smith, Gerry - Basic Sciences Division, Fred Hutchinson Cancer Research Center

 

Collection: Biology and Medicine

 
91 JOURNAL OF BACTERIOLOGY, 0021-9193/99/$04.00 0
 

Summary:  -Irradiated Escherichia coli Requires both Excision Repair and RecF Protein Function JUSTIN COURCELLE,* DAVID J. CROWLEY... that the disrupting lesions be removed. In the absence of excision repair, recF cells protect the nascent DNA... replication at UV doses from which wild-type cells recover efficiently, suggesting that recF and ...

  

Source: Courcelle, Justin - Department of Biology, Portland State University

 

Collection: Biology and Medicine

 
92 TB261 02/00 Novagen 1United States & Canada 800-207-0144 Germany 0800 6931 000
 

Summary:  Benzonase ® Nuclease TB261 02/00 Novagen 1United States & Canada 800-207-0144 Germany 0800 6931 000... U it d Ki d 0800 622935 Benzonase Nuclease Benzonase Nuclease, Purity >99% 10,000 U 70664... -3 Benzonase Nuclease, Purity >90% 10,000 U 70746-3 Benzonase is a genetically engineered endonuclease ...

  

Source: Lebendiker, Mario - Wolfson Centre for Applied Structural Biology, Hebrew University of Jerusalem

 

Collection: Biotechnology ; Biology and Medicine

 
93 84 BIOGENESIS AND ASSEMBLY OF MEMBRANE PROTEINS [6] that incorporation of radioactivity into hot trichloroacetic acid-insoluble
 

Summary:  by severance of the great vessels at the base of the heart or excision of the heart, and the pancreas... in another 50- ml aliquot of buffer A on ice. According to the size of the dog, the excised pancreas weighed... of load to cushion was about 3 : 1. The resulting pellet was resuspended in 20 ml of buffer B. Nuclease

  

Source: Walter, Peter - Department of Biochemistry and Biophysics, University of California at San Francisco

 

Collection: Biology and Medicine

 
94 Copyright 2004 by the Genetics Society of America RuvAB and RecG Are Not Essential for the Recovery of DNA Synthesis Following
 

Summary:  is incorporated, rearrangement if excision repair or bypassed by translesion DNA polymer- replication resumes from... the rep- repaired by nucleotide excision repair (Courcelle et lication fork DNA and limiting... the degradation of the al. 1999, 2003). Cells deficient in nucleotide excision nascent DNA by RecJ and Rec

  

Source: Courcelle, Justin - Department of Biology, Portland State University

 

Collection: Biology and Medicine

 
95 Genome Dyn Stab (3) R. Egel, D.-H. Lankenau: Recombination and Meiosis
 

Summary:  by the Spo11 nuclease, a meiosis-specific protein with homology to the Top6A subunit of archaeal type IIB... -terminal phosphoesterase motif that provides the single catalytic center for all its nuclease activities (see below... ). This nuclease func- tion is essential for Mre11 in meiosis, but in vegetative (somatic) cells, the ...

  

Source: Heyer, Wolf-Dietrich - Sections of Microbiology & Molecular and Cellular Biology, University of California, Davis

 

Collection: Biology and Medicine

 
96 Molecular Cell, Vol. 10, 15031509, December, 2002, Copyright 2002 by Cell Press Drosophila MUS312 Interacts with the
 

Summary:  - cation and characterization of the XPF-ERCC1 complex of human DNA repair excision nuclease. J. Biol. Chem... Interacts with the Nucleotide Excision Repair Endonuclease MEI-9 to Generate Meiotic Crossovers tains two... ). These proteins share a highly conserved nuclease domain (Aravind et al., 1999). A ...

  

Source: Sekelsky, Jeff - Department of Biology, University of North Carolina at Chapel Hill

 

Collection: Biology and Medicine

 
97 BATES, GINGER MUSE. Characterization of the protein component of Methanococcus jannaschii RNase P. (Under the direction of James W. Brown.)
 

Summary:  to nuclease treatment, whereas the holoenzyme from S. acidocaldarius has a buoyant density of 1.27g... /ml suggesting that it is largely made of protein and is insensitive to nuclease treatment (14,33). These two... in the form of a DEAD box helicase motif seen in ABC transporters (34). Organellar RNase P Of all RNase Ps

  

Source: Brown, James W. - Department of Microbiology, North Carolina State University

 

Collection: Environmental Sciences and Ecology ; Biology and Medicine

 
98 SURVEY AND SUMMARY Comparative analysis of editosome proteins in
 

Summary:  , contain two putative nuclease motifs. Thus, editosomes contain a plethora of nucleases or proteins presum... - ably derived from nucleases. Five additional related proteins, three of which have zinc ®ngers, each... are involved in eukaryotic base excision repair. The critical residues in DNA polymerase b ...

  

Source: Schnaufer, Achim - Institute of Immunology and Infection Research, University of Edinburgh

 

Collection: Biology and Medicine

 
99 SOLiDTM Total RNA-Seq Kit For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
 

Summary:  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24 Expected lengths of the insert and PCR product according to excised cDNA length . 25 Amplify the c... DNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 Expected lengths of the insert and PCR product ...

  

Source: Dratz, Edward A. - Department of Chemistry and Biochemistry, Montana State University

 

Collection: Biology and Medicine ; Chemistry

 
100 Intersubunit signaling in RecBCD enzyme, a complex protein machine regulated by Chi hotspots
 

Summary:  RecBCD helicase-nuclease, a paradigm of complex protein machines, initiates homologous genetic... that, in the mutant enzymes when RecD reaches the DNA end, it signals RecB's nuclease domain to cut... to the nuclease domain of RecB (Singleton et al., 2004). The steps between Chi recognition and alteration

  

Source: Smith, Gerry - Basic Sciences Division, Fred Hutchinson Cancer Research Center

 

Collection: Biology and Medicine


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