Home

About

Advanced Search

Browse by Discipline

Scientific Societies

E-print Alerts

Add E-prints

E-print Network
FAQHELPSITE MAPCONTACT US


  Topic List  Advanced Search  
Sample search results for: aav2 improves muscle

Page:   2  3  4  5 
 
1 Recombinant AAV Viral Vectors Pseudotyped with Viral Capsids from Serotypes 1, 2, and 5 Display
 

Summary:  @ufl.edu. Available online 1 July 2004 Recombinant adeno-associated virus 2 (rAAV2) has been shown to deliver genes... serotypes. The vectors contained AAV2 terminal repeats flanking a green fluorescent protein expression... cassette under the control of the synthetic CBA promoter, in AAV1, ...

  

Source: Kalil, Ronald E. - Neuroscience Training Program & W.M. Keck Laboratory for Biological Imaging, University of Wisconsin at Madison; Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
2 JOURNAL OF VIROLOGY, Aug. 2002, p. 76517660 Vol. 76, No. 15 0022-538X/02/$04.00 0 DOI: 10.1128/JVI.76.15.76517660.2002
 

Summary:  , respectively, for transduction of alveoli and airway epithelia (18, 19). AAV2/3 showed improved transduction... of smooth muscle cells in the mouse lung (18, 19). AAV5 was found to be more efficient than AAV2 at gene... transgene expression be- tween AAV5 and ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
3 JOURNAL OF VIROLOGY, Mar. 2002, p. 20432053 Vol. 76, No. 5 0022-538X/02/$04.00 0 DOI: 10.1128/JVI.76.5.20432053.2002
 

Summary:  protein expression, resulted in no improvement in the yield of the pseudopackaged rAAV-2cap5. Similarly... 2001 In the presence of complementing adeno-associated virus type 2 (AAV-2) Rep proteins, AAV-2 genomes... strategy, the involvement of the ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
4 JOURNAL OF VIROLOGY, 0022-538X/01/$04.00 0 DOI: 10.1128/JVI.75.16.76627671.2001
 

Summary:  compared the efficiency of gene delivery to mouse muscle cells for recombinant AAV type 2 (rAAV-2) and rAAV... the nonviral and viral vectors used in muscle gene transfer, recombinant adeno-associated virus type 2 (rAAV-2... has been examined in ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
5 original article 524 www.moleculartherapy.org vol. 17 no. 3, 524537 mar. 2009
 

Summary:  response in the second-injection site. In this study, we replicate our striatal rAAV2/2-GDNF... 2/2-GDNF readmin- istration, striatal rAAV2/2-GFP readministration leads to a loss of transgene... or the AAV2 capsid is the anti- genic stimulus in ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
6 Current Gene Therapy, 2003, 3, 281-304 281 1566-5232/03 $41.00+.00 2003 Bentham Science Publishers Ltd.
 

Summary:  the experience made over the last 20 years with AAV-2. Fortunately, all the basic improvements and tools... for the successful transition of AAV-2 vectors to human clinical trials was an improvement in methods for vector... Abstract: Gene transfer vectors based on ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
7 Capsid modifications overcome low heterogeneous expression of heparan sulfate proteoglycan that limits AAV2-mediated gene transfer and therapeutic
 

Summary:  improves transduction for OvCa cells Our data suggest that lack of HSPG expression is a barrier for AAV2... transduction of OvCa cells with the RGD-modified AAV2 capsid translated to improved gene-directed enzyme... that limits ...

  

Source: Hemminki, Akseli - Department of Biosciences, University of Helsinki

 

Collection: Biology and Medicine

 
8 Long-term Skeletal Muscle Protection After Gene Transfer in a Mouse Model of LGMD-2D
 

Summary:  analysis revealed that only those muscles injected with the rAAV2/1-tMCK-sgca construct expressed sgca... -/- mice. (Top left panel) sgca expression (green) in TA muscle injected with rAAV2/1-tMCK-sgca and Evans... muscles from the hindlimbs ...

  

Source: Campbell, Kevin P. - Department of Physiology and Biophysics, University of Iowa

 

Collection: Biology and Medicine

 
9 Expert Review Designer Gene Delivery Vectors: Molecular Engineering and Evolution
 

Summary:  muscle compared to AAV2/2 (83). In addition, AAV2/5 mediated efficient gene transfer to murine cerebellar... neurons (84), and AAV2/6 efficiently transduced skeletal muscle (85). Gao et al. have shown... that pseudotyped ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
10 JOURNAL OF VIROLOGY, Apr. 2004, p. 33613371 Vol. 78, No. 7 0022-538X/04/$08.00 0 DOI: 10.1128/JVI.78.7.33613371.2004
 

Summary:  . In contrast to AAV5, AAV2 does not bind sialic acid, but rather binds heparan sulfate proteo- glycans at its... phenotype common to all three viruses. In contrast, the overall surface topologies of AAV5 and AAV2... are similar. A pseudo-atomic model generated for AAV5 based on the crystal structure of ...

  

Source: Baker, Timothy S. - Department of Chemistry and Biochemistry, University of California at San Diego

 

Collection: Biology and Medicine

 
11 original article 2088 www.moleculartherapy.org vol. 17 no. 12, 20882095 dec. 2009
 

Summary:  , the enhanced transduction efficiencies of these AAV2- like mutants are likely due to improvements in either... as evidenced by the binding results for AAV4 (Figure 1) and the improved efficiencies of numerous AAV2- and AAV... , with the following order: ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
12 JOURNAL OF VIROLOGY, Apr. 2005, p. 50475058 Vol. 79, No. 8 0022-538X/05/$08.00 0 doi:10.1128/JVI.79.8.50475058.2005
 

Summary:  than AAV2. AAV1 has demonstrated an improved tro- pism for striated muscle cells compared to AAV2 (29... of its major capsid protein, VP3, with that of AAV2, to which AAV4 is 58% identical and constrained... for the ...

  

Source: Baker, Timothy S. - Department of Chemistry and Biochemistry, University of California at San Diego

 

Collection: Biology and Medicine

 
13 JOURNAL OF VIROLOGY, June 2004, p. 63446359 Vol. 78, No. 12 0022-538X/04/$08.00 0 DOI: 10.1128/JVI.78.12.63446359.2004
 

Summary:  . Circulating Anti-Wild-Type Adeno-Associated Virus Type 2 (AAV2) Antibodies Inhibit Recombinant AAV2 (rAAV2... -associated virus type 2 (AAV2), with 30% expressing neutralizing Ab (NAb). The blood-brain barrier (BBB) ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
14 HUMAN GENE THERAPY 18:367378 (April 2007) Mary Ann Liebert, Inc.
 

Summary:  optimization or scalability issues, and most AAV serotypes do not bind heparin or sialic acid, used for AAV2... such as the hexahistidine (His6) sequence. Through the combination of a diverse AAV2 library and rational peptide in... ) sequence. Through the combination of a diverse ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
15 Helper Virus-Free, Optically Controllable, and Two-Plasmid-Based Production of Adeno-associated
 

Summary:  - hibit host ranges largely different from AAV-2, as found in tissues such as skeletal muscle [6... -packaging of vector DNA derived from AAV-2, -3, or -6 was up to 10-fold more efficient using our novel plasmids... of pre- clinical studies and is supported by promising results from a ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
16 original article The American Society of Gene & Cell Therapy Molecular Therapy 1
 

Summary:  fluorescent protein (GFP) flanked by AAV2 inverted terminal repeats in the nonhuman primate basal ganglia... ). Six intact young adult cynomolgus monkeys received a single 10µl injection of AAV2/1-GFP, AAV2/5-GFP... , or ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
17 Systemic but Not Intraocular Epo Gene Transfer Protects the Retina from Light-and
 

Summary:  injected AAV2 vectors encoding either Epo or EGFP intraocularly and intramuscularly (im) into albino Lewis... rats and rds and rd10 mice. We injected the animals either intravitreally or subretinally with AAV2-CMV... -Epo in the right eye and performed control injections in the left eye. ...

  

Source: Sandini, Giulio - Dipartimento di Informatica Sistemistica e Telematica, Università degli Studi di Genova

 

Collection: Engineering ; Computer Technologies and Information Sciences

 
18 HUMAN GENE THERAPY 15:405413 (April 2004) Mary Ann Liebert, Inc.
 

Summary:  recombinant adenovirus (AdVLacZ), adeno-associated virus serotype 2 (AAV2LacZ), and pseudotyped adeno... efficiency of these four vectors in the pancreas of mice in vivo. We showed that adenovirus, AAV2, and AAV8... postdelivery, with greater than 90% of expression lost at 4 weeks. ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
19 Intracellular trafficking of adeno-associated viral and JF Engelhardt1,2,3
 

Summary:  viral genes are the inverted terminal repeats (ITRs, 145 bp in length for AAV2) at either end... as one of the primary attachment receptors for AAV2.39 To this end, soluble heparin can compete... for binding of AAV2 to the surface of many cell types.39,40 Mutagenesis ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
20 JOURNAL OF VIROLOGY, Mar. 2004, p. 31103122 Vol. 78, No. 6 0022-538X/04/$08.00 0 DOI: 10.1128/JVI.78.6.31103122.2004
 

Summary:  (AAV2) vectors is ineffi- cient; less than 10% of hepatocytes are permissive for stable transduction... , and transgene expression is characterized by a lag phase of up to 6 weeks. AAV2-based vector genomes packaged... of the liver with vectors based on AAV2 capsids ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine


Page:   2  3  4  5 
Page:   1  2  3  4  5 
 
21 JOURNAL OF VIROLOGY, Jan. 2005, p. 214224 Vol. 79, No. 1 0022-538X/05/$08.00 0 doi:10.1128/JVI.79.1.214224.2005
 

Summary:  tissues. However, with rAAV serotype 2 (rAAV2) vectors, liver transduction is confined to only a small... . The rAAV1 vector showed a blunted dose-response similar to that of rAAV2 at high doses, while the rAAV8... on AAV serotype 2 (rAAV2) have been most extensively ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
22 Copyright Lippincott Williams & Wilkins. Unauthorized reproduction of this article is prohibited. Gene therapy for hemophilia
 

Summary:  for dissemination of an AAV vector throughout muscle have been described. In the human muscle-directed AAV2 gene... demonstrates that use of genes that express modified human FIX proteins can improve the efficacy of AAV2-vector... delivery of ...

  

Source: Ponder, Katherine P. - Departments of Medicine & Biochemistry and Molecular Biophysics, Washington University in St. Louis

 

Collection: Biology and Medicine

 
23 JOURNAL OF VIROLOGY, June 2008, p. 58875911 Vol. 82, No. 12 0022-538X/08/$08.00 0 doi:10.1128/JVI.00254-08
 

Summary:  or individual residues. Notable examples include AAV-1/ AAV-2 chimeras with improved tropism in muscle tissue... performance in specific targets, exemplified by the AAV type 2 (AAV-2) prototype in the liver. Here, we... its closest natural relative ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
24 JOURNAL OF VIROLOGY, Jan. 2005, p. 364379 Vol. 79, No. 1 0022-538X/05/$08.00 0 doi:10.1128/JVI.79.1.364379.2005
 

Summary:  characterized by an AAV-2 and an AAV-5 ITR at opposite ends of the viral genome. This hybrid genome... was efficiently packaged into either AAV-2 or AAV-5 capsids to generate infectious virions. Hybrid AV2:5 ITR... have now been generated from eight different serotypes (3, 17), type 2 ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
25 original article The American Society of Gene Therapy Molecular Therapy vol. 16 no. 10, 17031709 oct. 2008 1703
 

Summary:  regions from AAV6, 8, and 9, all of which transduce heart and muscle significantly better than AAV2 (refs... mutagen- esis and selection, that successfully generated AAV2 vectors with novel gene-delivery properties... to yields that were only approximately fivefold lower than wild-type ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
26 HUMAN GENE THERAPY 16:781791 (July 2005) Mary Ann Liebert, Inc.
 

Summary:  Recombinant adeno-associated virus 2 (rAAV2) has been extensively used as a gene delivery vector... , 2003). rAAV2 has been the most widely used AAV serotype to deliver genes to the CNS (Kaplitt et al... ., 1998; Glatzel et al., 2000). rAAV2 transduces mostly neurons ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
27 Species-Specific Differences in Mouse and Human Airway Epithelial Biology of Recombinant
 

Summary:  nuclear uptake of rAAV2 and rAAV5, leading to significantly improved transduction (11). Proteasome... epithelia (rAAV2 rAAV5) as compared with mouse lung (rAAV5 rAAV2). It is unclear if these differences... biology. To this end, we compared ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
28 JOURNAL OF VIROLOGY, 0022-538X/00/$04.00 0
 

Summary:  controlling infection. In this study, we have examined endocytic and intracellular trafficking of AAV-2 using... that internalization of heparan sulfate proteo- glycan-bound AAV-2 requires V 5 integrin and activation of the small... pathway was necessary to initiate intracellular movement of ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
29 GENE THERAPY Preclinical in vivo evaluation of pseudotyped adeno-associated virus vectors
 

Summary:  far, those involving vectors derived from adeno-associated virus type 2 (AAV-2), a single... with the wild-type virus and vectors derived thereof, and the feasibility to produceAAV-2 vector particles... at high titer and purity.6 Preclinical analyses of AAV-2 vectors ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
30 Construction of diverse adeno-associated viral libraries for directed evolution of enhanced gene
 

Summary:  extension process. The resulting PCR product is then cloned into pSub2 to generate a diverse (>106) AAV2... plasmid library. Finally, the AAV2 plasmid library is used to package a diverse pool of mutant AAV2... and nonhuman serotypes have been isolated, but only ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
31 Unique Biologic Properties of Recombinant AAV1 Transduction in Polarized Human Airway Epithelia*
 

Summary:  . In the present study, we sought to compare the biologic properties of rAAV2/1, rAAV2/2, and rAAV2/5 transduction... method. Results demonstrated that apical transduction of human airway epithelia with rAAV2/1 was 100-fold... ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
32 Directed evolution of adeno-associated virus yields enhanced gene delivery vectors
 

Summary:  for improvement from the first to the second round. (b) A molecular model of the full AAV2 capsid, based... (AAV) 2 variants with enhanced properties. High-throughput selection processes were designed to isolate... serotypes have been identified2­5; of these, ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
33 A Two-hybrid Screen Identifies Cathepsins B and L as Uncoating Factors for Adeno-associated Virus 2
 

Summary:  with the corresponding portion of the AAV2 capsid in yeast, but not with AAV5. We demonstrate that cathepsins B and L... are essential for efficient AAV2- and AAV8-mediated transduction of mammalian cells, and document the ability... of purified cathepsin B and L proteins to bind and cleave intact ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
34 JOURNAL OF VIROLOGY, Mar. 2005, p. 36063614 Vol. 79, No. 6 0022-538X/05/$08.00 0 doi:10.1128/JVI.79.6.36063614.2005
 

Summary:  mutagenesis and the mechanisms of rAAV integration. Recombinant adeno-associated virus serotype 2 (rAAV2... animals and human subjects (14). rAAV2 vectors have the ability to inte- grate into host chromosomes... VII mice treated with a rAAV2 vector developed liver ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
35 Characterization of the Relationship of AAV Capsid Domain Swapping to Liver
 

Summary:  Intestine Muscle AAV2 0.4 <0.1b <0.1 <0.1 ndc <0.1 nd <0.1 AAV8 6.6 nd nd <0.1 nd nd <0.1 nd DS1 1.0 <0.1 <0... over the widely used but less efficient serotype AAV2, even though the capsid amino acid sequences... genes contain- ing exchanged domains between ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
36 original article The American Society of Gene & Cell Therapy Molecular Therapy 1
 

Summary:  recombinant adeno-associated virus type 2/1 (rAAV2/1), to provide neuroprotection and functional restoration... to adult striatal PTN expression and approximated peak endogenous developmental levels (P1). rAAV2/1 vector... striatum using recombinant adeno-associated virus 2/1 ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
37 Heparin binding induces conformational changes in Adeno-associated virus Hazel C. Levy a
 

Summary:  Heparin sulfate a b s t r a c t Adeno-associated virus serotype 2 (AAV2) uses heparan sulfate proteoglycan... as a cell surface-attachment receptor. In this study the structures of AAV2 alone and complexed with heparin... density in the interior of the capsid in the ...

  

Source: Baker, Timothy S. - Department of Chemistry and Biochemistry, University of California at San Diego

 

Collection: Biology and Medicine

 
38 REVIEW ARTICLE Prevention of spontaneous bleeding in dogs with haemophilia
 

Summary:  VR, Stedman HH, Nichols TC et al. Regional intravascular delivery of AAV-2-F.IX to skeletal muscle... FVIIa Bleeds per year post gene transfer 1. Herzog et al. 1999 [17] B45 B M AAV-2-CMV-cFIX i.m. 1.3 · 1011 vp... kg)1 16 20 ± 5 2.6 ± 0.7 0.3 B46 B M ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
39 HUMAN GENE THERAPY 18:8187 (January 2007) Mary Ann Liebert, Inc.
 

Summary:  ). Heterodimerization of AAV2 genomes has been studied ex- tensively in mouse skeletal muscle (Yang et al., 1999; Sun et... ). In comparison with homologous AAV2 or AAV5 ITR vectors, hybrid 2:5-ITR vec- tors have been shown to less... sequences de- rived from ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
40 Dual Therapeutic Utility of Proteasome Modulating Agents for Pharmaco-Gene Therapy of the
 

Summary:  agents (LLnL and doxorubicin) at the time of rAAV2 or rAAV2/5 infection dramatically enhanced CFTR gene... of CF patients [16­19]. Although recent trials with rAAV2 have demonstrated an impressive safety profile... to date not detected CFTR transgene expression in ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine


Page:   1  2  3  4  5 
Page:   1  2  3  4  5 
 
41 JOURNAL OF VIROLOGY, July 2003, p. 73617366 Vol. 77, No. 13 0022-538X/03/$08.00 0 DOI: 10.1128/JVI.77.13.73617366.2003
 

Summary:  -Strand Genome Conversion of Adeno-Associated Virus Type 2 (AAV-2) and AAV-5 Is Not Rate Limiting following... apical infection. In contrast, rAAV-2 has been shown to be inherently ineffective at transducing airway... enhance rAAV-2 transduction from the apical ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
42 Current Gene Therapy, 2007, 7, 347-360 347 1566-5232/07 $50.00+.00 2007 Bentham Science Publishers Ltd.
 

Summary:  serotype (AAV2) resulting in circulating neutralizing antibodies [Blacklow et al., 1968; Blacklow et al... ., 1971; Chirmule et al., 1999; Erles et al., 1999]. Immune responses to rAAV2 have been reported... response when injecting lower titers of rAAV2 vectors (2 x ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
43 COLE DOCTORALE : DES GNOMES AUX ORGANISMES UNIVERSIT D'VRY VAL D'ESSONNE
 

Summary:  . ...............................................................................................................................................42 Figure 29 ­ Sécrétion muscle-spécifique de mSeAP lors de l'injection d'AAV2 et d'adjuvant p... ................................................................................ 32 b) ...

  

Source: Ecole Polytechnique, Centre de mathématiques

 

Collection: Mathematics

 
44 Successful transduction of liver in hemophilia by AAV-Factor IX and limitations imposed by the host
 

Summary:  al. Regional intravascular delivery of AAV-2-F.IX to skeletal muscle achieves long-term correction... that rAAV-2 vectors can transduce human hepatocytes in vivo to result in therapeutically relevant levels... , T cell­mediated immunity to AAV capsid antigens causes destruction of ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
45 Current Gene Therapy, 2001, 1, 137-147 137 1566-5232/00 $20.00+.00 2001 Bentham Science Publishers Ltd.
 

Summary:  : Despite the fact that adeno-associated virus type 2 (AAV2) is an extremely attractive gene therapy vector... strategies capable of overcoming intracellular barriers to AAV2 transduction. The basis of these technologic... by AAV2 remains very inefficient (Duan et ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
46 Lucas et al. Comparative transductions of breast cancer cells by three DNA viruses
 

Summary:  serotype 2 (CAV-2) adenovirus, and a human serotype 2 adeno-associated virus (AAV-2) in breast cancer cells... . We observed an excellent infection efficiency for Ad5 vector, whereas both CAV-2 and AAV-2 vectors... promising types of vectors (Ad5, CAV-2 and AAV-2) in ...

  

Source: Ecole Polytechnique, Centre de mathématiques

 

Collection: Mathematics

 
47 original article 2114 www.moleculartherapy.org vol.15no.12,21142123dec.2007
 

Summary:  with three rAAV sero- types (AAV1, AAV2, AAV5). These findings suggest that the Indian Rhesus monkey may... lung,12 and liver.21,22 Pre-clinical studies in NHP lung using rAAV2-CFTR virus have clearly... of bioelectric properties and susceptibil- ity to rAAV infection with rAAV1, ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
48 Transposon-Based Mutagenesis Generates Diverse Adeno-Associated Viral Libraries with Novel Gene
 

Summary:  . Briefly, a selectable marker is randomly inserted throughout the AAV2 cap gene and the resulting... alternatives to the well-characterized and most clinically utilized AAV serotype 2 (AAV2) (8,9), numerous... identified amino acids critical to AAV2 function ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
49 Systemic Mannitol-Induced Hyperosmolality Amplifies rAAV2-Mediated Striatal Transduction to a
 

Summary:  Systemic Mannitol-Induced Hyperosmolality Amplifies rAAV2-Mediated Striatal Transduction... (rAAV2) have been investigated as highly effective vehicles for gene transfer to the central nervous... system (CNS). Transduction with rAAV2 vectors results in long-term ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
50 Recombinant Adeno-associated Viral Vectors as Therapeutic Agents to Treat Neurological Disorders
 

Summary:  in the substantia nigra using rAAV vectors might be a promising strategy [71]. Indeed, rAAV2 vectors display... and cholesterol within distended lyso- somes throughout the brain. Using an ASM knockout mouse model and a rAAV2... , and contralateral hippocampus consistent with retrograde axonal transport of ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
51 JOURNAL OF VIROLOGY, Jan. 2006, p. 426439 Vol. 80, No. 1 0022-538X/06/$08.00 0 doi:10.1128/JVI.80.1.426439.2006
 

Summary:  genotype and helper serotype improved the replication of the respective gfp cassette, compared to the AAV-2... with adeno-associated virus 8 (AAV-8) pseudotypes, i.e., AAV-2 genomes packaged into AAV-8 capsids. Here we... antibodies against the AAV prototype, ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
52 Adeno-associated viral vectors and stereotaxic
 

Summary:  to differences in capsid proteins #12;Tissue Liver AAV8, AAV9, AAVDJ Skeletal Muscle AAV1, AAV6-9 CNS AAV1, AAV2... , AAV5, AAV8, AAV9, AAVDJ Photoreceptor Cells AAV5 Lung AAV9 Heart AAV8 Pancreas AAV8 Kidneys AAV2... ;Serotype: AAV2 Protein ...

  

Source: Chapman, Michael S. - Department of Biochemistry and Molecular Biology, Oregon Health and Science University

 

Collection: Biology and Medicine

 
53 Directed evolution of adeno-associated virus to an infectious respiratory virus
 

Summary:  of improved transduction, apical binding was analyzed. Recombinant AAV2.5T bound to the apical surface... distinct receptors, AAV2 (heparan sulfate) and AAV5 (sialic acid) (13­16). Subsequent selection... combined 2 divergent serotypes that use distinct receptors, ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
54 JOURNAL OF VIROLOGY, Oct. 2006, p. 98319836 Vol. 80, No. 19 0022-538X/06/$08.00 0 doi:10.1128/JVI.00878-06
 

Summary:  to be in part due to its ability to uncoat more readily than other AAV serotypes such as AAV2, understanding all... a role in transduction by three other closely related serotypes (AAV2, -3, and -9). Sequence and deletion... (typically derived from the AAV serotype 2 ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
55 BioMed Central Page 1 of 12
 

Summary:  Pseudotyped AAV vectors were generated by packaging AAV2-based recombinant genomes in AAV1, AAV2 or AAV5... XX6 [44], a pAAV packaging plasmid expressing the rep and cap genes (pACG2.1 for AAV2, pLT-RC02... for AAV1 and pLT- RC03 for AAV5) and the relevant ...

  

Source: Paris-Sud XI, Université de - Institut d'Optique, Laboratoire Charles-Fabry, Groupe d'Optique Atomique

 

Collection: Physics

 
56 446 MOLECULAR THERAPY Vol. 2, No. 5, November 2000 Copyright The American Society of Gene Therapy
 

Summary:  .RSV-gal) or a recombinant AAV serotype 2 (rAAV2) vector (vTR-CMV) mixed with wild-type adenovirus type 5 (Ad5). One week... throughout the injected hemisphere. Using an rAAV2 vector transducing a CMV-regulated green fluorescent... of transduced cells. Injection of ...

  

Source: Low, Walter C. - Department of Integrative Biology and Physiology, University of Minnesota

 

Collection: Biology and Medicine

 
57 ORIGINAL ARTICLE Surface immobilization of hexa-histidine-tagged
 

Summary:  that used AAV2-mediated gene delivery to the retina for treatment of Leber's congenital amaurosis... . Specifically, we had previously inserted a hexa-histidine (6xHis) onto a physically exposed loop of the AAV2... either wild-type (wt) AAV2 or 6xHis tag capsids) to ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
58 Sustained Striatal Ciliary Neurotrophic Factor Expression Negatively Affects
 

Summary:  by recombinant adeno-associated viral vectors (rAAV2). Wild-type and R6/1 HD transgenic (R6/1) mice that received... bilateral or unilateral intrastriatal injections of rAAV2-CNTF experienced weight loss. The CNTF- treated R6... to express a secretable form of CNTF and pack- aged into viral particles ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
59 Introduction Cystic fibrosis (CF) is the most common inherited dis-
 

Summary:  enhance rAAV-2­mediated gene transfer in muscle, rat cardiac muscle and mouse skeletal muscle were... , not only with rAAV-2, but also with all other types of viral vectors currently in use (1, 2). Several... proteoglycan receptor (3), and FGFR-1 ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
60 Long-Term Restoration of Rod and Cone Vision by Single Dose rAAV-Mediated Gene Transfer to the Retina
 

Summary:  . Results with AAV 2/2, 2/1, and 2/5 vector pseudotypes, human or canine RPE65 cDNA, and constitutive... received a surgically delivered subretinal injection of recombinant adeno-associated virus serotype 2 (AAV2... . Vector: 1, AAV2/2-CBA- cRPE65; 2, ...

  

Source: Palczewski, Krzysztof - Department of Pharmacology, Case Western Reserve University

 

Collection: Biology and Medicine


Page:   1  2  3  4  5 
Page:   1  2  3  4  5 
 
61 Enhanced Preparation of Adeno-Associated Viral Vectors by Using High Hydrostatic Pressure to
 

Summary:  serotypes 2 and 5 (AAV2, AAV5), which were previously unknown, were characterized. Adenovirus... was inactivated beyond detection at 260 MPa or higher, whereas AAV2 was stable up to $450 MPa, and surprisingly... , AAV5 was stable up to at least 700 MPa. The viral genomic DNA of pressure-inactivated ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
62 A Novel Adeno-Associated Viral Variant for Efficient and Selective Intravitreal Transduction of Rat Muller Cells
 

Summary:  significantly improved transduction relative to AAV2 (.60%) and AAV6. Conclusions/Significance: Our findings... dependences found among AAV serotypes. AAV2 shows strong dependence on heparan sulfate proteoglycans (HSPG... in vitro) with several distinct libraries: (1) an ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
63 HUMAN GENE THERAPY 16:11161123 (September 2005) Mary Ann Liebert, Inc.
 

Summary:  epithelia were infected from the apical mem- brane with rAAV2 or rAAV5 CFTR-PTM vectors in the presence... . Levels of CFTR correction seen with rAAV2 (1.07 0.24 A) and rAAV5 (0.90 0.20 A) CFTR-PTM vectors were... -CF epithelia, and was similar for both rAAV2 and rAAV5 ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
64 JOURNAL OF VIROLOGY, Mar. 2004, p. 28632874 Vol. 78, No. 6 0022-538X/04/$08.00 0 DOI: 10.1128/JVI.78.6.28632874.2004
 

Summary:  - tion and transduction of recombinant adeno-associated virus type 2 (rAAV-2) and rAAV-5 serotypes... polarized epithelia with either rAAV-2 or rAAV-5. In summary, the cell type specificity of LLn... lung disease with rAAV type 2 (rAAV-2) have demonstrated a promising ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
65 [23] Adeno-Associated Virus Vectors for Short Hairpin RNA Expression
 

Summary:  facts and numbers presented refer to AAV serotype 2 (AAV-2), which is considered the prototype... the transgene (here labeled as shRNA) flanked by the AAV-2 ITRs, (2) an AAV helper plasmid encoding the rep... serotype (Erles et al., 1999). This detail may have led to the very early definition of ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
66 Molecular Engineering of Viral Gene Delivery Vehicles
 

Summary:  of the resulting variants were functional, and an AAV1 domain that conferred enhanced muscle tropism to AAV2... and nonhuman tissues (34, 35). Most studies to date have focused on AAV serotype 2 (AAV2), but recently several... cotransfection of a noninfectious ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
67 PEG Conjugation Moderately Protects Adeno-Associated Viral Vectors Against
 

Summary:  ; Nakai et al., 1999). Over 90% of the human population is seropositive for anti-AAV2 antibodies... to advance serotypes other than AAV2 to the clinic until they are better characterized. Furthermore, even... to design strategies to protect AAV2 from neutralization by ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
68 Neurotechnique Reversible Silencing of Neuronal Excitability in
 

Summary:  constructs were commercially packaged and serotyped with the AAV2 capsid protein (Virapur, Inc.), yielding... titers of 1012 particles per ml. We refer to the viruses encoding the a and b subunit as AAV2-a and AAV2... . The suspension contained either ...

  

Source: Lester, Henry - Division of Biology, California Institute of Technology

 

Collection: Biology and Medicine

 
69 Robust Systemic Transduction with AAV9 Vectors in Mice: Efficient Global Cardiac Gene Transfer Superior
 

Summary:  vector that overcomes many of the limitations of rAAV2 and transduces various tissues efficiently... (rAAV2) is a promising vector, in vivo gene transfer with rAAV2 is suboptimal in many instances... . To overcome such drawbacks of ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
70 HUMAN GENE THERAPY 18:344354 (April 2007) Mary Ann Liebert, Inc.
 

Summary:  ;LUNG GENE TRANSFER USING AAV6 VECTORS 345 airway expression from an AAV2 vector is consistent... with the low level of heparan sulfate proteoglycans, a receptor for AAV2, on the apical surface of the airway... ) and is an abundant sugar on the apical sur- face, consistent with the better performance of AAV5 ...

  

Source: Miller, Dusty - Division of Human Biology, Fred Hutchinson Cancer Research Center

 

Collection: Biology and Medicine

 
71 Current Gene Therapy, 2009, 9, 375-388 375 1566-5232/09 $55.00+.00 2009 Bentham Science Publishers Ltd.
 

Summary:  ]. Along these lines, AAV capsid immunogenicity has been a particular problem when using the AAV2 capsid... of transgene expression in the AAV2 trials performed to date. However, capsid serotypes that are proc- essed... more rapidly than AAV2 appear to be less immuno- ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
72 JOURNAL OF VIROLOGY, 0022-538X/99/$04.00 0
 

Summary:  for molecular medicine. Recent identification of the viral receptor and corecep- tors for AAV type 2 (AAV-2) has... with this vector. However, the mechanisms by which AAV-2 enters cells remain unknown. In the present report, we... have examined whether the endocytic pathways of ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
73 NATURE MEDICINE VOLUME 8 NUMBER 3 MARCH 2002 253 Duchenne muscular dystrophy (DMD) is the most common form
 

Summary:  improvement over mdx diaphragms (Table 1). Furthermore, TA muscles from the R4­R23 and R2­R21+H3 micro... transgenic mdx mice with muscles that were not measurably different from wild type. This improvement... vectors. Micro-dystrophin cDNAs were cloned into the ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
74 JOURNAL OF VIROLOGY, 0022-538X/00/$04.00 0
 

Summary:  that is resistant to gene transfer with AAV (2). Differen- tiated epithelia were infected with recombinant AAV type... 2 (rAAV2) which was produced by a CaPi cotransfection proto- col and was purified through three... plasmid pCisAv.GFP3ori was used to generate rAAV2 ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
75 Trans-splicing vectors expand the utility of adeno-associated virus for gene therapy
 

Summary:  . First, the AAV-2 cell membrane receptor, heparan sulfate proteoglycan, as well as two corecep- tors... adenovirus and wild-type AAV2 was determined as previously described (7) and was less than one functional... erythropoietin protein was expressed in muscle subsequent to the formation ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
76 SHORT COMMUNICATION Comparative biology of rAAV transduction in ferret,
 

Summary:  AAV serotypes closely mirrors that observed in human epithelia (rAAV14 rAAV2DrAAV5), while transduction of mouse... epithelia was significantly different (rAAV14rAAV5brAAV2). Similarly, ferret, pig and human epithelia also... transgene expression in the airway epithe- lium using ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
77 Molecular Vision 2005; 11:1236-45 <http://www.molvis.org/molvis/v11/a141/> Received 5 July 2005 | Accepted 7 December 2005 | Published 30 December 2005
 

Summary:  with two different recombinant adeno-associated virus (AAV) serotypes (AAV2/2 andAAV2/ 5) in utero... of AAV2/2.CMV.EGFP, or AAV2/ 5.CMV.EGFPwere performed in utero at E14 in C57Bl/6 mice [4]; the same... subretinally by ...

  

Source: Sandini, Giulio - Dipartimento di Informatica Sistemistica e Telematica, Università degli Studi di Genova

 

Collection: Engineering ; Computer Technologies and Information Sciences

 
78 Sites in the AAV5 capsid tolerant to deletions and tandem duplications Kaoru Hida a
 

Summary:  in clinical trials with recombinant AAV serotype 2 (rAAV2) vectors [6,7], there still remain a number... receptors for AAV2, are widespread, leading to difficulties in achieving good tissue disper- sion... identified sites amenable to insertion of foreign peptides [13­16] as well as sites critical for ...

  

Source: Gray, Jeffrey J. - Department of Biomolecular and Chemical Engineering, Johns Hopkins University; Ostermeier, Marc - Department of Biomolecular and Chemical Engineering, Johns Hopkins University

 

Collection: Biology and Medicine ; Biotechnology ; Chemistry

 
79 MOLECULAR THERAPY Vol. 4, No. 4, October 2001 Copyright The American Society of Gene Therapy
 

Summary:  on the molecular fate of the rAAV2 genome in skeletal muscle. Proc. Natl. Acad. Sci. USA 98: 4084­4088. 32... Z (from nt 1027 to 2299) between pCisLacZUpstream and pCisLacZDownstream. Recombinant AAV-2 production... ]. Briefly, 60% confluent 293 cells were co-transfected with a cis ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
80 Variable gearing in pennate muscles Emanuel Azizi*, Elizabeth L. Brainerd, and Thomas J. Roberts
 

Summary:  measurements may represent a previously unrecognized mechanism for improving muscle performance over a range... ratio is variable, muscle performance can be improved for both fast and forceful contractions. Given... Variable gearing in pennate muscles Emanuel Azizi*, Elizabeth L. ...

  

Source: Brainerd, Elizabeth - Department of Ecology and Evolutionary Biology, Brown University

 

Collection: Environmental Sciences and Ecology


Page:   1  2  3  4  5 
Page:   1  2  3  4  5 
 
81 Supplementary Appendix This appendix has been provided by the authors to give readers additional information about their work.
 

Summary:  of AAV2.hRPE65v2. Reduction in nystagmus may account for the improved visual acuity in the uninjected... of AAV2.hRPE65v2 was performed at The Children's Hospital of Philadelphia for all subjects. After... ,3 the #12;2 eye with worse function was selected for delivery of ...

  

Source: Gleeson, Joseph G. - Department of Neurosciences, University of California at San Diego

 

Collection: Biology and Medicine

 
82 original article 2096 www.moleculartherapy.org vol. 17 no. 12, 20962102 dec. 2009
 

Summary:  and survival of retinal neurons. Intravitreal administration could cir- cumvent this problem, but only AAV2 can... several AAV serotype capsids and fol- lowed their retinal distribution after intravitreal injection. AAV2... for some AAV serotypes, and AAV2 uses ...

  

Source: Long, Jeffrey R.- Department of Chemistry, University of California at Berkeley

 

Collection: Materials Science ; Chemistry

 
83 GENE THERAPY AAV-mediated factor IX gene transfer to skeletal muscle in patients with
 

Summary:  we hypothesized that slow fibers of human muscle are preferentially transduced by rAAV-2. Pruchnic et... ). The expression cassette is flanked by AAV-2 ITRs. (B-D) Total gDNAwas isolated from biopsied muscle tissue... al showed abundant presence of HSPGs, which act as ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
84 JOURNAL OF VIROLOGY, Apr. 2004, p. 41654175 Vol. 78, No. 8 0022-538X/04/$08.00 0 DOI: 10.1128/JVI.78.8.41654175.2004
 

Summary:  rAAV2 as the serotype for testing gene correction in vivo. In tibialis muscle, the onset of gene... - gene cassette were cloned into a rAAV2 proviral plasmid to create various truncation mutants... according to the base location of truncation in the eGFP gene relative to the starting ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
85 MOLECULAR THERAPY Vol. 6, No. 3, September 2002 Copyright The American Society of Gene Therapy
 

Summary:  -dependent pro- tein kinase on the molecular fate of the rAAV2 genome in skeletal muscle. Proc. Natl. Acad. Sci... (rAAV2) Vectors Renius Owen IV, Ronald J. Mandel, Chandramohan V. Ammini, Thomas J. Conlon, Douglas S... are depicted. ITR, AAV2 ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
86 Simulation Lab #2: Dynamic Modeling and Simulation of Muscle-Tendon Actuators
 

Summary:  Simulation Lab #2: Dynamic Modeling and Simulation of Muscle-Tendon Actuators Professor B.J. Fregly... properties of muscle are complex, highly nonlinear and can have substantial effects on movement (see Mc... Mahon 1984 for review). For simplicity, lumped-parameter dimensionless muscle models, capable of representing

  

Source: Fregly, B. J. - Department of Mechanical and Aerospace Engineering, University of Florida

 

Collection: Engineering ; Biology and Medicine

 
87 JOURNAL OF VIROLOGY, Oct. 2007, p. 1129011303 Vol. 81, No. 20 0022-538X/07/$08.00 0 doi:10.1128/JVI.00963-07
 

Summary:  from mouse liver, skeletal muscle, and heart. rAAV2 and rAAV8 vectors with AAV-ISce I.AO3 genomes were... .8 SCID AAV2 30 PV Liver 86 82 2 79 95.3 SCID AAV8 720 TV Muscle 189 161 5 148 85.2 SCID AAV8 720 TV Heart... .4 5.5 14.9 B6 ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
88 JOURNAL OF VIROLOGY, 0022-538X/99/$04.00 0
 

Summary:  the A and A regions had occurred. To create a second hybrid vector (Ad.AAV2) (Fig. 1), the AAVSNori cassette developed... were analyzed for integrated AAV provirus by Southern analysis of genomic DNA by using the AAV2 wild... obtained from pALSAPSN, the 4.5-kb BsaI/SnaBI fragment of pAAV/Ad containing the ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
89 2003 NaturePublishing Group 346 | MAY 2003 | VOLUME 4 www.nature.com/reviews/genetics
 

Summary:  was recently shown in haemophilia B patients after muscle-directed gene transfer of an AAV2 vector20... -associated viruses(AAV) Most rAAV vectors have been derived fromAAV2,but,so far,a total of eight distinct... ­49 .Pseudotyping the rAAV2 ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
90 This article was published in an Elsevier journal. The attached copy is furnished to the author for non-commercial research and
 

Summary:  and extremely high titer pure virus is commonly obtained (Zolotukhin et al., 2002). rAAV2 has been the most... ). The receptor for AAV2 has been characterized and is composed of the heparan sulfate proteoglycan receptor... and Samulski, 1998). The relative abundance of these receptors in the brain probably ...

  

Source: Mandel, Ronald J. - Department of Neuroscience, University of Florida

 

Collection: Biology and Medicine

 
91 brief report The new engl and jour nal of medicine
 

Summary:  adverse-event pro- file after delivery of AAV2.hRPE65v2. Each patient had a modest improvement in measures... with LCA2 who received AAV2.hRPE65v2 by subretinal injection showed evidence of improvement in retinal... ,10-12 Recombinant ...

  

Source: Gleeson, Joseph G. - Department of Neurosciences, University of California at San Diego

 

Collection: Biology and Medicine

 
92 doi:10.1182/blood-2010-08-302729 Prepublished online November 24, 2010;
 

Summary:  %) with the use of the vectorAAV2-hFIX16.1 Although AAV vectors are predominantly nonintegrating, with most... serotype 2 (AAV2) vector encoding -glucuronidase in neonatal mice resulted in a significant increase... performed liver- directed gene transfer with the single-stranded ...

  

Source: Bushman, Frederic - Department of Microbiology, University of Pennsylvania

 

Collection: Biology and Medicine

 
93 The idea of gene-based therapeutics has been around for some time, but it only received serious attention
 

Summary:  natural humoral adeno-associated virus 2 (AAV2)-specific antibodies as a result of naturally occurring... . The prototype AAV2 vector, which is based on a human-derived virus, can be pseudotyped with one of various AAV... trials. One of the most notable trials includes retinal infusion of an ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
94 Architectural Properties of Distal Forelimb Muscles in Horses, Equus caballus
 

Summary:  improve locomotor efficiency (Biewener, 1998). To date, a complete ar- chitectural profile of the muscles... Architectural Properties of Distal Forelimb Muscles in Horses, Equus caballus Nicholas A.T. Brown,1... . To accurately and noninvasively predict muscle and joint contact forces, a detailed model of ...

  

Source: Meyers, Ron - Department of Zoology, Weber State University

 

Collection: Environmental Sciences and Ecology ; Biology and Medicine

 
95 BioMed Central Page 1 of 15
 

Summary:  of the i.p. injection method could be improved when compared to the one of the muscle local procedure... Research article Optical imaging of luminescence for in vivo quantification of gene electrotransfer in mouse muscle... cranial muscle electrotransfer of a luciferase encoding plasmid. Optical imaging was ...

  

Source: Ecole Polytechnique, Centre de mathématiques

 

Collection: Mathematics

 
96 HUMAN GENE THERAPY 16:299306 (March 2005) Mary Ann Liebert, Inc.
 

Summary:  serotype 2 (AAV-2). However, when this vector is delivered at high doses into the portal vein or hepatic... for adeno-associated vector serotype 2 (AAV-2) vectors show promise for gene therapy trials. We established... al., 2003). Among those, adeno- associated vector serotype 2 ...

  

Source: Kay, Mark A. - Department of Genetics, Stanford University

 

Collection: Biology and Medicine

 
97 Introduction Fish vary in terms of swimming form and can employ a
 

Summary:  and recruitment of the various myotomal muscle fiber types. Red, slow-twitch fibers provide the propulsive energy... ., 1984). At higher swimming speeds, the pink and/or white muscle fibers are recruited (Coughlin and Rome... but fatigue readily in most fish species (Webb, 1994). Red, pink and white muscles represent fiber types

  

Source: Coughlin, David J. - Science Division, Widener University

 

Collection: Environmental Sciences and Ecology

 
98 Virus Research 104 (2004) 5159 Novel approaches to augment adeno-associated virus
 

Summary:  -limiting steps in rAAV type-2 (rAAV-2) transduction. Several strategies have been developed to enhance rAAV-2... AAV transduction by enhancing endocytosis of rAAV-2. A selective trypsinization assay demonstrated... pathway to enhance uptake of ...

  

Source: Engelhardt, John F. - Department of Anatomy and Cell Biology, University of Iowa

 

Collection: Biology and Medicine

 
99 Directed evolution for drug and nucleic acid delivery Kaoru Hida a
 

Summary:  were inserted into an AAV2 at a position previously identified as an efficient site for the insertion... a variety of related techniques to rapidly evolve peptides and proteins into new forms that exhibit improved... been recently described, including those that improve the effectiveness of therapeutic ...

  

Source: Gray, Jeffrey J. - Department of Biomolecular and Chemical Engineering, Johns Hopkins University; Ostermeier, Marc - Department of Biomolecular and Chemical Engineering, Johns Hopkins University

 

Collection: Biology and Medicine ; Biotechnology ; Chemistry

 
100 Journal of Biomechanics 39 (2006) 13831391 ASB 2004 Pre-Doctoral Young Scientist Award
 

Summary:  of these muscles. These new representations of muscle architecture can improve the accuracy of computer simulations... capture the effects of complex features of muscle architecture, can improve the accuracy of computer... femoris and vastus intermedius fiber excursions predicted by ...

  

Source: Blemker, Silvia Salinas - Department of Mechanical and Aerospace Engineering, University of Virginia; Stanford University - Department of Bioengineering, Skeletal Mechanobiology Research Grouop

 

Collection: Biology and Medicine ; Engineering


Page:   1  2  3  4  5