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Sample search results for: abasic endonuclease action

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1 Oligonucleotides with Bistranded Abasic Sites Interfere with Substrate Binding and Catalysis by Human Apurinic/Apyrimidinic Endonuclease
 

Summary:  stronger inhibitor of enzyme action than an oligonucleotide containing the corresponding abasic site... Oligonucleotides with Bistranded Abasic Sites Interfere with Substrate Binding and Catalysis... by Human Apurinic/Apyrimidinic Endonuclease Jose A. Mckenzie and Phyllis R. Strauss* Department of Biology

  

Source: Strauss, Phyllis R. - Department of Biology, Northeastern University

 

Collection: Biology and Medicine

 
2 Human Apurinic/Apyrimidinic Endonuclease Is Processive, Daniel C. Carey and Phyllis R. Strauss*
 

Summary:  Human Apurinic/Apyrimidinic Endonuclease Is Processive, Daniel C. Carey and Phyllis R. Strauss... Vised Manuscript ReceiVed October 7, 1999 ABSTRACT: Apurinic/apyrimidinic endonuclease (AP endo) is believed... abasic sites in the base excision repair pathway. AP endo makes a single nick 5 to an abasic site

  

Source: Strauss, Phyllis R. - Department of Biology, Northeastern University

 

Collection: Biology and Medicine

 
3 Submitted to J. Biol. Chem. Draft of July 13, 2001
 

Summary:  /apyrimidinic endonuclease; ASR, abasic site repair, bp, base pair; ds, double-stranded; ESR, electron spin resonance; HDP... -ligated through the action of a DNA ligase. The cleaved abasic site can also be displaced by one of several DNA... Submitted to J. Biol. Chem. Draft of July 13, 2001 Characterization of ...

  

Source: Strauss, Phyllis R. - Department of Biology, Northeastern University

 

Collection: Biology and Medicine

 
4 Abasic Site Repair in Higher Eukaryotes Phyllis R. Strauss and Noreen E. O'Regan
 

Summary:  3 Abasic Site Repair in Higher Eukaryotes Phyllis R. Strauss and Noreen E. O'Regan 1. INTRODUCTION... of abasic sites generated per mammalian cell per day run as high as 106/cell/d (88). Abasic sites... the large number of abasic sites generated per cell per day, the number of resulting mutations is extremely

  

Source: Strauss, Phyllis R. - Department of Biology, Northeastern University

 

Collection: Biology and Medicine

 
5 Clustered DNA damages induced in isolated DNA and in human cells by low doses of ionizing radiation
 

Summary:  (48­51) E. coli Nfo protein (endonuclease IV) Abasic sites Several types of abasic sites, including... bases and AP endonuclease action) generates de novo DSBs at cluster sites (block 4). (D) DNA molecules... , abasic sites, or oxidized bases) on opposing ...

  

Source: Brookhaven National Laboratory, Environmental Chemistry Division, Department of Applied Science

 

Collection: Environmental Sciences and Ecology

 
6 DNA Repair 3 (2004) 14471455 Novel role of tyrosine in catalysis by human AP endonuclease 1
 

Summary:  , to the abasic site. Tyr171 and Tyr128 have been identified as important for enzy- matic action [24], although... human abasic endonuclease: new insights from EDTA-resistant incision of acyclic abasic site analogs... of the role of the histidine-aspartate pair in the human exonuclease ...

  

Source: Strauss, Phyllis R. - Department of Biology, Northeastern University

 

Collection: Biology and Medicine

 
7 Domain Mapping of Human Apurinic/Apyrimidinic Endonuclease STRUCTURAL AND FUNCTIONAL EVIDENCE FOR A DISORDERED AMINO TERMINUS AND A TIGHT
 

Summary:  Domain Mapping of Human Apurinic/Apyrimidinic Endonuclease STRUCTURAL AND FUNCTIONAL EVIDENCE... /apyrimidinic endonuclease (AP endo). In this report we describe the domain struc- ture of the enzyme in solution determined... , became strikingly less accessible to protease digestion in the presence of abasic site-containing DNA

  

Source: Strauss, Phyllis R. - Department of Biology, Northeastern University

 

Collection: Biology and Medicine

 
8 Multiple Cleavage Activities of Endonuclease V from Thermotoga maritima: Recognition and Strand Nicking Mechanism
 

Summary:  the uracil base and with AP endonucleases and other enzymes to repair the subsequent abasic site (4, 5... , an inducible AP endonuclease in E. coli, recognizes a doubly flipped AP site; i.e., both the abasic sugar... Multiple Cleavage Activities of Endonuclease V from Thermotoga maritima: ...

  

Source: Barany, Francis - Biochemistry and Structural Biology Program, Weill Cornell Graduate School of Medical Sciences

 

Collection: Biology and Medicine

 
9 3-Methyladenine DNA glycosylases: structure,
 

Summary:  to produce an abasic site. The abasic site is then processed by one of two pathways, termed short... generates a DNA strand break at the abasic site and the abasic terminus is removed by deoxyribo- phosphatase... by a replicative polymerase.AP endonucle- ase generates a strand break at the ...

  

Source: Lau, Albert Y. - Department of Biophysics and Biophysical Chemistry, Johns Hopkins University

 

Collection: Biology and Medicine

 
10 Oxidative DNA Base Damage by the Antitumor Agent 3-Amino-1,2,4-benzotriazine 1,4-Dioxide (Tirapazamine)
 

Summary:  . DNA repair endonucleases are useful tools for the ana- lysis of DNA damage.23,24 Used in this context... ) and endonuclease IV (endo IV) reveal damage to the DNA backbone by converting 0960-894X/02/$ - see front matter... (endo IV), and abasic sites (exo III and endo IV) into strand breaks (Fig. 1).23,25,26 The base excision

  

Source: Gates, Kent. S. - Departments of Chemistry & Biochemistry, University of Missouri-Columbia

 

Collection: Chemistry

 
11 DNA base excision repair activities and pathway function in mitochondrial and cellular lysates from
 

Summary:  endonuclease IV, to ensure complete cleavage of abasic sites. Reactions were terminated by addition of 20 ml... supplemented the BER assay of mitochondrial lysates with pure uracil DNA glycosylase, AP endonuclease and... . While nuclear BER protein levels and activities were generally not altered in r0 cells, AP ...

  

Source: Stuart, Jeffrey A. - Department of Biological Sciences, Brock University

 

Collection: Biology and Medicine

 
12 Molecular Cell, Vol. 20, 367375, November 11, 2005, Copyright 2005 by Elsevier Inc. DOI 10.1016/j.molcel.2005.09.018 MRE11/RAD50 Cleaves DNA
 

Summary:  generated by successive action of AID and UNG2, be- cause it is the major abasic endonuclease active in base... in diversifying Ig genes. In principle, the major abasic endonuclease APE1 could also attack these AP sites... by deamination of cytosine to uracil, followed by removal of ...

  

Source: Maizels, Nancy - Department of Biochemistry, University of Washington at Seattle

 

Collection: Biology and Medicine

 
13 Substrate Binding by Human Apurinic/Apyrimidinic Endonuclease Indicates a Briggs-Haldane Mechanism*
 

Summary:  Apurinic/apyrimidinic endonuclease (AP endo) makes a single nick 5 to a DNA abasic site. We have character... endonuclease cleavage of a 49-base pair oligonucleotide with a single abasic site Steady-state kinetic... Substrate Binding by Human Apurinic/Apyrimidinic Endonuclease Indicates a ...

  

Source: Strauss, Phyllis R. - Department of Biology, Northeastern University

 

Collection: Biology and Medicine

 
14 MOLECULAR AND CELLULAR BIOLOGY, Sept. 2002, p. 64806486 Vol. 22, No. 18 0270-7306/02/$04.00 0 DOI: 10.1128/MCB.22.18.64806486.2002
 

Summary:  endonuclease activity and functions in the removal of abasic sites from DNA. Here, we provide evidence... cerevisiae ho- molog of the major human AP endonuclease HAP1, and its role in the repair of abasic sites... activity of human apurinic endonuclease (Ape) at ...

  

Source: Burgers, Peter M. - Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis

 

Collection: Biology and Medicine

 
15 Notes & Tips A nonisotopic assay for unambiguous assignment of DNA
 

Summary:  but leave be- hind an abasic or apurinic/apyrimidinic (AP)1 site in DNA [4]. Thus, for the analysis... strand scission adjacent to the abasic site. The lack of current DNA glycosylase assays to concur- rently... detect abasic DNA products and AP-lyase products has likely been a contributing factor in controversies

  

Source: Boston University, Center for Advanced Biotechnology

 

Collection: Biotechnology

 
16 Molecular Cell, Vol. 13, 751762, March 12, 2004, Copyright 2004 by Cell Press Snapshots of Replication through
 

Summary:  of Replication through an Abasic Lesion: Structural Basis for Base Substitutions and Frameshifts and Dbh (Din... B homolog) from two related Sulfolobus strains are able to extend primer strands past abasic sites... an abasic le- right-hand-like structure. The thumb and little finger do-sion. We have determined crystal

  

Source: Ling, Hong - Department of Biochemistry, University of Alberta

 

Collection: Biology and Medicine

 
17 Base Excision and DNA Binding Activities of Human Alkyladenine DNA Glycosylase Are Sensitive to the Base Paired with a Lesion*
 

Summary:  affinity of human alkylad- enine DNA glycosylase to a DNA product containing an abasic site was similar... 1 -N glycosylic bond, forming a free DNA base and an abasic sugar residue. Once the damaged base... 16% of the sub- strates were converted to abasic DNA products during the time course of the EMSA

  

Source: Lau, Albert Y. - Department of Biophysics and Biophysical Chemistry, Johns Hopkins University

 

Collection: Biology and Medicine

 
18 Effect of Protein Binding on Ultrafast DNA Dynamics: Characterization of a DNA:APE1 Complex
 

Summary:  that lacks endonuclease activity, but retains the ability to bind to abasic sites (29,30). In a second set... . Demple. 1995. Incision activity of human apurinic endonuclease (APE) at abasic site analogs in DNA. J... and specific amino acid residues in the catalytic reaction of the major human ...

  

Source: Coleman, Robert S. - Department of Chemistry, Ohio State University

 

Collection: Chemistry

 
19 JOURNAL OF BACTERIOLOGY, July 2002, p. 35013507 Vol. 184, No. 13 0021-9193/02/$04.00 0 DOI: 10.1128/JB.184.13.35013507.2002
 

Summary:  and the deoxyribose, resulting in an abasic (AP) site. Subsequently, AP endonucleases incise the DNA backbone... by the action of AP endonucleases. Alternatively, un- cleaved AP sites may be less easily converted... , including formation of deaminated and oxidized bases. Apurinic/apyrimidinic (AP) ...

  

Source: Engelward, Bevin - Division of Bioengineering, Massachusetts Institute of Technology (MIT)

 

Collection: Biology and Medicine

 
20 Single-Turnover Analysis of Mutant Human Apurinic/Apyrimidinic Endonuclease, Julie A. Lucas, Yuji Masuda,|, Richard A. O. Bennett,| Nathaniel S. Strauss,,@ and Phyllis R. Strauss*,
 

Summary:  and cleavage of abasic site-containing DNA. Apurinic/apyrimidinic endonuclease (AP endo) is a human enzyme... Single-Turnover Analysis of Mutant Human Apurinic/Apyrimidinic Endonuclease, Julie A. Lucas,§ Yuji... Ved February 8, 1999 ABSTRACT: Apurinic/apyrimidinic endonuclease (AP endo) is a key enzyme in the ...

  

Source: Strauss, Phyllis R. - Department of Biology, Northeastern University

 

Collection: Biology and Medicine


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21 Genome Biology 2004, 5:211 commentreviewsreportsdepositedresearchinteractionsinformationrefereedresearch
 

Summary:  endonucleases to nick the abasic site so that new DNA synthe- sis can recreate the duplex [6]. Reasoning... removes U, leaving an abasic site. Subsequent steps generate single-strand breaks [17], which become... , and that uracil-DNA glyco- sylase removes U to produce an abasic site (see shaded box in Figure ...

  

Source: Maizels, Nancy - Department of Biochemistry, University of Washington at Seattle

 

Collection: Biology and Medicine

 
22 Characterization of DNA Damage Induced by a Natural Product Antitumor Antibiotic Leinamycin in Human Cancer Cells
 

Summary:  , 1­12. (24) Wilson, D. M., III., and Barsky, D. (2001) The major human abasic endonuclease: Formation... ., and Wilson, D. M., III. (2008) Characterization of abasic endonuclease activity of human Ape1 on alternative... checkpoint mechanisms irrespective of their mode of actions (50). ...

  

Source: Gates, Kent. S. - Departments of Chemistry & Biochemistry, University of Missouri-Columbia

 

Collection: Chemistry

 
23 Z .Mutation Research 460 2000 201210 www.elsevier.comrlocaterdnarepair
 

Summary:  then expose the target nu- cleotide and cleave the glycosylic bond. The result- ing abasic site is processed... by a repair endonuclease ) Corresponding author. Z .E-mail address: tome@hms.harvard.edu T. Ellenberger... A protein is a member of the Helix-hairpin-Helix family of glycosylases that in- cludes the endonuclease III

  

Source: Lau, Albert Y. - Department of Biophysics and Biophysical Chemistry, Johns Hopkins University

 

Collection: Biology and Medicine

 
24 Homologous recombination prevents methylation-induced toxicity in Escherichia coli
 

Summary:  resulting from AP-endonuclease action. INTRODUCTION Multiple DNA repair systems specific for alkylation... not shown) which is consistent with previous studies and confirms that AP-endonuclease action is important... arise in methylated DNA by at least two mechanisms. First, AlkA or Tag glycosylase ...

  

Source: Engelward, Bevin - Division of Bioengineering, Massachusetts Institute of Technology (MIT)

 

Collection: Biology and Medicine

 
25 JOURNAL OF BACTERIOLOGY, 0021-9193/01/$04.00 0 DOI: 10.1128/JB.183.1.131138.2001
 

Summary:  /apurinic (AP) endonucleases at the abasic site. This results in a 3 -OH terminus, which can act as a primer... ]) showed essentially wild-type levels of NO resistance. However, apyrimidinic/apurinic (AP) endonuclease... of abasic sites is critical for defense against NO. In addition, recA mutant cells were ...

  

Source: Engelward, Bevin - Division of Bioengineering, Massachusetts Institute of Technology (MIT)

 

Collection: Biology and Medicine

 
26 BioMed Central Page 1 of 6
 

Summary:  the DNA leaving an abasic site where the strand is nicked at both the 3' and 5' ends [6]. The abasic site... the remaining C. The action of MutM brings the number of adenines A misincorpo- rated opposite 8-oxo-G during... Y [9,10], which excises the mispaired adenine A leaving an abasic site. The ...

  

Source: Lunds Universitet, - Computational Biology and Biological Physics Group

 

Collection: Physics ; Biology and Medicine

 
27 Mitochondrial and nuclear DNA base excision repair are affected differently by caloric restriction1
 

Summary:  ) and processing of the resulting abasic site by AP endonuclease. These activities were measured as percent... at a defined position in the oligonucleotide), processing of the abasic site, incorporation of the new... in AP endonuclease and polymerase activities in mitochondria The first steps in the repair of ...

  

Source: Stuart, Jeffrey A. - Department of Biological Sciences, Brock University

 

Collection: Biology and Medicine

 
28 DEMETER DNA Glycosylase Establishes MEDEA Polycomb Gene Self-Imprinting
 

Summary:  -glycosylic bond, creating an abasic site, whereas the lyase activity nicks the DNA. An AP endonuclease generates... ) and an abasic site at 18 (top strand). Re- actions were performed, terminated by addition of NaOH, boiled... of the phospho- diester bonds (Figure 3A). Cleavage 50 to the ...

  

Source: Goldberg, Robert B. - Department of Molecular, Cell, and Developmental Biology, University of California at Los Angeles

 

Collection: Biology and Medicine

 
29 Direct Real Time Observation of Base Flipping by the EcoRI DNA Methyltransferase*
 

Summary:  for the binding of the methyltransferase to DNA containing abasic sites or base analogs incorpo- rated... parameters and are cleaved by the EcoRI endonuclease (28­30). Using a steady-state 2AP-based base flipping... with the EcoRI endonuclease (data not shown). M.EcoRI concentration (108 M) was determined spectrophoto

  

Source: Reich, Norbert O. - Department of Chemistry and Biochemistry, University of California at Santa Barbara

 

Collection: Chemistry ; Biology and Medicine

 
30 Localization of mitochondrial DNA base excision repair to an inner membrane-associated
 

Summary:  of the abasic site following uracil removal even in fractions deficient in AP endonuclease activity (r0... includes four distinct steps: lesion removal by a glycosylase, abasic site processing by an apurinic... /apyrimidinic (AP) endonuclease, insertion of a new nucleotide by polymerase g and ligation of the ...

  

Source: Stuart, Jeffrey A. - Department of Biological Sciences, Brock University

 

Collection: Biology and Medicine

 
31 Advances in Engineering Homing Endonucleases for GeneTargeting
 

Summary:  Chapter 6 Advances in Engineering Homing Endonucleases for GeneTargeting: TenYears After Structures... Barry L Stoddard,, Andrew M Scharenberg and Raymond J Monnat, Jr§ Homing endonucleases (HEs) are highly... site-specific endonucleases that induce homologous recombination or gene conversion in vivo by cleaving

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
32 Mutational Analysis of Endonuclease V from Thermotoga maritima Jianmin Huang,, Jing Lu,,,| Francis Barany, and Weiguo Cao*,,|
 

Summary:  Mutational Analysis of Endonuclease V from Thermotoga maritima Jianmin Huang,,§ Jing Lu... Ved NoVember 19, 2001; ReVised Manuscript ReceiVed April 28, 2002 ABSTRACT: Endonuclease V nicks damaged... DNA at the second phosphodiester bond 3 to inosine, uracil, mismatched bases, or abasic (AP) sites

  

Source: Barany, Francis - Biochemistry and Structural Biology Program, Weill Cornell Graduate School of Medical Sciences

 

Collection: Biology and Medicine

 
33 Homingendonucleasestructureandfunction Barry L. Stoddard
 

Summary:  endonucleases are encoded by open reading frames that are embedded within group I, group II and archael introns... of intron and intein mobility. Homing endonucleases can be divided into several unique families... , and they display disparate DNA cleavage mechanisms. A significant number of homing endonucleases also act

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
34 Ubiquitinated proliferating cell nuclear antigen activates translesion DNA polymerases and REV1
 

Summary:  , the flap endonuclease FEN1, and DNA ligase I. However, whereas the activity of DNA polymerase remains... is a deoxycytidyl transferase that shows the highest catalytic activity opposite template guanines and abasic sites... (14, 15). Rev1 is primarily responsible for inserting dC residues opposite abasic sites during

  

Source: Burgers, Peter M. - Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis

 

Collection: Biology and Medicine

 
35 Holliday junctions in the eukaryotic nucleus: resolution in sight?
 

Summary:  resolvases in the eukaryotic nucleus have long eluded identifi- cation. The endonucleases Mus81/Mms4-Eme1... in crossover formation in budding yeast and flies, respect- ively. Although these endonucleases might represent... resolution to non-cross- over products can also be achieved topologically, for example, by the action of ...

  

Source: Heyer, Wolf-Dietrich - Sections of Microbiology & Molecular and Cellular Biology, University of California, Davis

 

Collection: Biology and Medicine

 
36 Effect of lesions on the dynamics of DNA on the picosecond and nanosecond timescales using a
 

Summary:  of the abasic site is important in its recognition by the endonuclease (46,47). The second lesion studied... is the ¯ipping of the abasic sugar out of the helix and the concomitant intrusion of water into the interior... DNA: an abasic site and a helix terminus. Because these comparisons are looking for ...

  

Source: Coleman, Robert S. - Department of Chemistry, Ohio State University

 

Collection: Chemistry

 
37 An Overview of Chemical Processes That Damage Cellular DNA: Spontaneous Hydrolysis, Alkylation, and Reactions with Radicals
 

Summary:  Backbone 1749 2.4. Properties of Abasic Sites Arising from Depurination 1749 3. Overview: Common Reactions... 's attention to sites where the "action" is occurring rather than to illustrate complete sequences of bond... ). The oxocarbenium ion undergoes subsequent hydrolysis to yield an abasic site 7 (often referred to as an ...

  

Source: Gates, Kent. S. - Departments of Chemistry & Biochemistry, University of Missouri-Columbia

 

Collection: Chemistry

 
38 Mitochondrial DNA maintenance and bioenergetics Jeffrey A. Stuart , Melanie F. Brown
 

Summary:  the first step of this pathway, a DNA glycosylase removes the damaged base leaving an abasic site. AP-endonuclease... [56], and a mitochon- drial endonuclease specific for apurinic/apyrimidinc sites was identified... with streptozotocin, an alkylating antibiotic [63]. Complete repair of abasic sites has been ...

  

Source: Stuart, Jeffrey A. - Department of Biological Sciences, Brock University

 

Collection: Biology and Medicine

 
39 DNA Binding and Cleavage by the HNH Homing Endonuclease I-HmuI
 

Summary:  DNA Binding and Cleavage by the HNH Homing Endonuclease I-HmuI Betty W. Shen1 , Markus Landthaler2... , Albany, NY 12222 USA The structure of I-HmuI, which represents the last family of homing endonucleases... -binding surface found in the I-PpoI homing endonuclease and an associated HNH/N active site found in the bacterial

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
40 This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research
 

Summary:  t Apurinic/apyrimidinic endonuclease 1 (APE1) is a multifunctional enzyme with a well-established abasic DNA... established abasic DNA endonuclease function. Kinetic parameters of APE1, RNase A, and RNase If To further... III, Characterization of abasic endonuclease ...

  

Source: Rader, Stephen - Chemistry Program, University of Northern British Columbia

 

Collection: Biology and Medicine ; Chemistry


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41 University of Maryland Baltimore Graduate School Announcement of Doctoral Dissertation Defense
 

Summary:  of these damaged bases result in the formation of an abasic or AP site, allowing the next enzyme in the pathway... , Apurinic/Apyrimidinic Endonuclease 1 (APE1), to hydrolytically cleave the DNA backbone 5' to the AP site

  

Source: Weber, David J. - Department of Biochemistry and Molecular Biology, University of Maryland-Baltimore

 

Collection: Biology and Medicine

 
42 ANRV260-GE39-03 ARI 21 June 2005 22:14 Immunoglobulin Gene
 

Summary:  , abasic endonucleases nick the phosphodi- ester backbone at the abasic site, DNA poly- merase synthesizes... containing abasic sites, including abasic endonucleases like APE1, which cleave the phosphodiester back- bone... -fidelity DNA synthesis (translesion ...

  

Source: Maizels, Nancy - Department of Biochemistry, University of Washington at Seattle

 

Collection: Biology and Medicine

 
43 Chemistry & Biology, Vol. 12, 357369, March, 2005, 2005 Elsevier Ltd All rights reserved. DOI 10.1016/j.chembiol.2004.12.011 Delineation of the Chemical Pathways Underlying
 

Summary:  of guanine leads to 8-oxoguanine and its secondary ox- idation products, 8-nitroguanine, as well as abasic... double-strand breaks by direct reaction with DNA [32, 34, 35]. How- ever, base lesions, abasic sites... an associated AP lyase ac- tivity and by AP endonucleases [36]. Consequently, if DNA glycosylases initiate BER

  

Source: Engelward, Bevin - Division of Bioengineering, Massachusetts Institute of Technology (MIT)

 

Collection: Biology and Medicine

 
44 Pure Appl. Chem., Vol. 83, No. 3, pp. 677686, 2011. doi:10.1351/PAC-CON-10-09-28
 

Summary:  on noncovalent interactions between a spin-labeled nucleobase and an abasic site in duplex DNA. Keywords: EPR... function, i.e., their mechanism of action. In addition to satisfying academic curiosity about life itself... Postsynthetic spin labeling of nucleobases by click chemistry: Detection of abasic sites The success

  

Source: Sigurdsson, Snorri Thor - Chemistry Division, Science Institute, University of Iceland.

 

Collection: Chemistry

 
45 DNA Distortion and Specificity in a Sequence-Specific Endonuclease
 

Summary:  DNA Distortion and Specificity in a Sequence-Specific Endonuclease Andrea C. Babic1 , Elizabeth J... : restriction endonuclease; indirect readout; protein-DNA complex Introduction Many fundamentally important... ). Restriction endonucleases are a class of enzymes found in bacteria that cleave DNA at specific target

  

Source: Horton, Nancy C. - Department of Chemistry and Biochemistry, University of Arizona

 

Collection: Biology and Medicine ; Chemistry

 
46 A Ubiquitin-binding Motif in the Translesion DNA Polymerase Rev1 Mediates Its Essential Functional Interaction with
 

Summary:  and abasic sites (30, 31). This enzyme is primarily respon- sible for inserting dC residues opposite abasic... by pol , the flap endonuclease FEN1, and DNA ligase I (18). Translesion synthesis by pol is stimulated... such on the template that Rev1 has to insert one dCMP opposite a template guanine prior to encountering the ...

  

Source: Burgers, Peter M. - Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis

 

Collection: Biology and Medicine

 
47 Chemical Properties of the Leinamycin-Guanine Adduct Tony Nooner, Sanjay Dutta, and Kent S. Gates*
 

Summary:  . Res. 461, 83-108. (35) Wilson, D. M., III, and Barsky, D. (2001) The major human abasic endonuclease... ) containing either 3 or 7.5 units of Aag endonuclease (Trevigen). The solutions were incubated at 37 °C... cytotoxicity. Our results indicate that leinamycin is an efficient reagent for the rapid generation of ...

  

Source: Gates, Kent. S. - Departments of Chemistry & Biochemistry, University of Missouri-Columbia

 

Collection: Chemistry

 
48 news and views Type II restriction endonucleases have
 

Summary:  news and views Type II restriction endonucleases have served as models for understanding the basis... restriction endonucleases by reporting the structure of the enzyme BglII complexed to its cognate DNA target... site (AGATCT). There are a variety of classes of restric- tion endonucleases. They are grouped

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
49 SURVEY AND SUMMARY Comparative analysis of editosome proteins in
 

Summary:  ®ed protein motifs associ- ated with catalysis and protein or nucleic acid inter- actions that suggest... are related and share an AP endonuclease motif (6). Two closely related editosome proteins (TbREL1 and TbREL2... .oxfordjournals.orgDownloadedfrom #12;and TbMP99 that can catalyze pre-cleaved (independent of endonuclease) in ...

  

Source: Schnaufer, Achim - Institute of Immunology and Infection Research, University of Edinburgh

 

Collection: Biology and Medicine

 
50 Molecular basis for discriminating between normal and damaged bases by the human alkyladenine
 

Summary:  , and the resulting abasic nucleotide is excised and replaced with a normal nucleotide by the sequential action... of an endonuclease, a polymerase, and DNA ligase (14). The high selectivity for damaged vs. normal bases is essential... , the pyrrolidine abasic nucleotide (pyr; PDB ID code 1bnk; refs. 15 and ...

  

Source: Lau, Albert Y. - Department of Biophysics and Biophysical Chemistry, Johns Hopkins University

 

Collection: Biology and Medicine

 
51 Isolation and Characterization of New Homing Endonuclease Specificities at Individual Target Site
 

Summary:  Isolation and Characterization of New Homing Endonuclease Specificities at Individual Target Site... Pomona College, 609 North College Avenue, Claremont CA 91711, USA Homing endonucleases are highly... specific DNA endonucleases, encoded within mobile introns or inteins, that induce targeted recombination

  

Source: Monnat, Ray - Department of Genome Sciences, University of Washington at Seattle; Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
52 int. j. radiat. biol 2001, vol. 77, no. 2, 155 164 DNA strand break yields after post-high LET irradiation
 

Summary:  irradiation incubation with endonuclease-III and evidence for hydroxyl radical clustering J. R. MILLIGAN*, J... is that tion of plasmid DNA with the endonuclease-III (endo-III) of multiple chemical modi cations of DNA... . These endonucleases have the prop- ously was the use of chambers in the form of anerty of removing certain ...

  

Source: Nevis Laboratories, Radiological Research Accelerator Facility

 

Collection: Fission and Nuclear Technologies ; Biology and Medicine

 
53 Cell, Vol. 95, 249258, October 16, 1998, Copyright 1998 by Cell Press Crystal Structure of a Human Alkylbase-DNA
 

Summary:  ., 1997; David and Williams, 1998). The resultingBoston, Massachusetts 02115 abasic (AP) sites are further... of DNA, causing to DNA containing a transition state mimic of the glyco- the abasic pyrrolidine... nucleotide to flip into the en- sylase reaction, a pyrrolidine abasic nucleotide, which zyme active site

  

Source: Lau, Albert Y. - Department of Biophysics and Biophysical Chemistry, Johns Hopkins University

 

Collection: Biology and Medicine

 
54 DNA Repair 1 (2002) 645659 The S. cerevisiae Mag1 3-methyladenine DNA glycosylase
 

Summary:  is then cleaved 5 to the abasic sugar by the major AP endonuclease, Apn1, which is responsi- ble for >95... known. Uncleaved abasic sites inhibit DNA replication [49], and AP endonuclease deficient cells show in... induced by uncleaved abasic sites in AP ...

  

Source: Engelward, Bevin - Division of Bioengineering, Massachusetts Institute of Technology (MIT)

 

Collection: Biology and Medicine

 
55 Stimulation of RTH1 Nuclease of the Yeast Saccharomyces cereVisiae by Replication Protein A
 

Summary:  in the function of RTH1 nuclease, including its structure specific endonuclease activity. Initial in Vitro studies... oligonucleotides. Yeast RPA stimulated the endonuclease activity of RTH1 endonuclease with these substrates... endonuclease substrates indicated that RPA has a weak helix destabilizing effect ...

  

Source: McQuade, D. Tyler - Department of Chemistry and Biochemistry, Florida State University

 

Collection: Chemistry

 
56 The restriction fold turns to the dark side: a bacterial homing endonuclease with
 

Summary:  through the action of restric- tion endonucleases. I-Ssp6803I uses its tetrameric assem- bly to promote... The restriction fold turns to the dark side: a bacterial homing endonuclease with a PD... The homing endonuclease I-Ssp6803I causes the insertion of a group I intron into a bacterial tRNA gene

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
57 Engineered Extrahelical Base Destabilization Enhances Sequence Discrimination of DNA Methyltransferase
 

Summary:  completely when the target cytosine is replaced with an abasic sugar.23 Thus, Val121 is important... is replaced with an abasic site (Table 2). F84A is decreased dramatically in catalysis as well (Table 3, 1000... affinity using abasic DNA with F84A demonstrates a direct coupling of the loop positioning and target

  

Source: Reich, Norbert O. - Department of Chemistry and Biochemistry, University of California at Santa Barbara

 

Collection: Chemistry ; Biology and Medicine

 
58 DNA damage recognition and repair by 3-methyladenine DNA glycosylase I (TAG)
 

Summary:  structures of Salmonella typhi TAG in the unliganded form and in a ternary product complex with abasic DNA... to other glycosylase-DNA structures, the abasic ri- bose is not flipped into the TAG active site... are the crystal structures of Salmonella typhi TAG alone and in complex with abasic DNA and 3mA, together

  

Source: Eichman, Brandt F. - Department of Biological Sciences, Vanderbilt University

 

Collection: Biology and Medicine

 
59 Molecular Cell Short Article
 

Summary:  Molecular Cell Short Article Structure of the Endonuclease Domain of MutL: Unlicensed to Cut Monica... lever spanning the conserved endonuclease motif. Additional conserved motifs cluster around the lever... describing how the interaction with MutS and the processivity clamp could license the endonuclease activity

  

Source: Guarne, Alba - Department of Biochemistry and Biomedical Sciences, McMaster University

 

Collection: Biology and Medicine

 
60 Molecular Cell, Vol. 15, 607620, August 27, 2004, Copyright 2004 by Cell Press The Fanconi Anaemia Gene FANCC Promotes
 

Summary:  approach to identifyenously generated abasic sites. Moreover in these ver- the type of repair defect in FA... -SceI endonuclease. This is displayed as the percentage of cells that become GFP positive as detected by FACS... for the repair of endogenously generated abasic sites in theseen in human FA cells. FANCC knockout (KO) ...

  

Source: Patel, KJ - Laboratory of Molecular Biology, MRC

 

Collection: Biology and Medicine


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61 Crystal Structure of Type IIE Restriction Endonuclease Eco RII Reveals an Autoinhibition Mechanism by a
 

Summary:  Crystal Structure of Type IIE Restriction Endonuclease Eco RII Reveals an Autoinhibition Mechanism... -10098 Berlin, Germany Eco RII is a type IIE restriction endonuclease that interacts with two copies... cleft. The C-terminal catalytic domain has a restriction endonuclease-like fold. Structure

  

Source: Chen, Liqing - Department of Chemistry, University of Alabama in Huntsville

 

Collection: Chemistry ; Biotechnology

 
62 Function and Evolution of HO and VDE Endonucleases in Fungi
 

Summary:  Function and Evolution of HO and VDE Endonucleases in Fungi James E. Haber, Kenneth H. Wolfe 1... Introduction The site-specific HO and VDE endonucleases are unusual members of a fam- ily of so-called group I... LAGLIDADG homing endonucleases that are general- ly implicated in the homing of intron and intein sequences

  

Source: Wolfe, Kenneth H. - Genetics Department, Trinity College, University of Dublin

 

Collection: Biotechnology ; Environmental Sciences and Ecology

 
63 Template-independent ligation of single-stranded DNA by T4 DNA ligase
 

Summary:  containing an abasic site or a gap at the ligation junction, joins branched DNA strands, and forms a stem... adjacent sites for the specific restriction endonuclease, and two shorter fragments a and b resulting from... endonuclease before PCR amplification should yield such a single amplicon (Fig. 1). Because the restriction

  

Source: Boston University, Center for Advanced Biotechnology

 

Collection: Biotechnology

 
64 CTMI (2006) 301:283315 c Springer-Verlag Berlin Heidelberg 2006
 

Summary:  of the remaining phosphodeoxyribose residue. Through the concerted actions of APE endonuclease and DNA polymerase... enzymes involved, namely the endonuclease Vsr in Escherichia coli and two members of the uracil DNA... and eukaryotes have different cleavage sites. Vsr is an endonuclease, cleaving the ...

  

Source: Tian, Weidong - Institute of Biochemistry and Cell Biology, Shanghai Institute of Biological Sciences

 

Collection: Biology and Medicine

 
65 Flexible DNA Target Site Recognition by Divergent Homing Endonuclease Isoschizomers I-CreI
 

Summary:  Flexible DNA Target Site Recognition by Divergent Homing Endonuclease Isoschizomers I-CreI and I... University of Washington, Seattle WA 98195 USA Homing endonucleases are highly specific catalysts of DNA... strand breaks that induce the transposition of mobile intervening sequences containing the endonuclease

  

Source: Aydin, Zafer - Department of Genome Sciences, University of Washington at Seattle; Monnat, Ray - Department of Genome Sciences, University of Washington at Seattle; Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
66 P E R S P E C T I V E S www.sciencemag.org SCIENCE VOL 306 19 NOVEMBER 2004 1299
 

Summary:  transcripts are cleaved by endonucleases to generate the 3 ends of mRNAs, tRNAs, microRNAs, and certain small... . A serendipitous clue to the identity of the mRNA endonuclease comes from studies of tRNA processing. Mutations... in the ELAC2 gene appear to cause suscepti- bility to prostate cancer (7). ELAC2 is an endonuclease

  

Source: Wickens, Marv - Department of Biochemistry, University of Wisconsin at Madison

 

Collection: Biology and Medicine

 
67 BS200 Biochemistry Experiment 2008 Fall Instructor Professor Changwon Kang (Biological Sciences)
 

Summary:  DNA isolation and characterization by electrophoresis Text Exp. 15 The action of restriction... endonucleases on plasmid Text Exp. 3 Using gel filtration to study ligandprotein interaction Text Exp. 4

  

Source: Kim, Eunjoon - Department of Biological Sciences, Korea Advanced Institute of Science and Technology

 

Collection: Biology and Medicine

 
68 Gene 206 (1998) 1121 Degenerate DNA recognition by I-PpoI endonuclease
 

Summary:  Gene 206 (1998) 11­21 Degenerate DNA recognition by I-PpoI endonuclease Paula K. Wittmayer... 1997; accepted 14 September 1997; Received by C.M. Kane Abstract The I-PpoI endonuclease is encoded... substrate specificities of I-PpoI and other homing endonucleases make these enzymes valuable tools

  

Source: Raines, Ronald T. - Departments of Biochemistry & Chemistry, University of Wisconsin at Madison

 

Collection: Chemistry ; Biology and Medicine

 
69 Biochemical Studies of the Site-Specific I-Ppol Endonuclease
 

Summary:  Biochemical Studies of the Site-Specific I-Ppol Endonuclease by Paula Kathleen Wittmayer... ENDONUCLEASE Paula Kathleen Wittmayer Under the supervision of Associate Professor Ronald T. Raines... At the University ofWisconsin-Madison IMPpol endonuclease, an intronMencoded endonuclease from the slime mold

  

Source: Raines, Ronald T. - Departments of Biochemistry & Chemistry, University of Wisconsin at Madison

 

Collection: Chemistry ; Biology and Medicine

 
70 Structures of the Rare-Cutting Restriction Endonuclease NotI Reveal a Unique Metal
 

Summary:  Structure Article Structures of the Rare-Cutting Restriction Endonuclease NotI Reveal a Unique... in a variety of putative endonucleases, occupied by an iron atom coordinated within a tetra- hedral Cys4 motif... represent an evolutionary intermedi- ate between mobile endonucleases (which recognize longer target sites

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
71 The Rockefeller University Press, 0021-9525/2000/07/F31/6 $5.00 The Journal of Cell Biology, Volume 150, Number 2, July 24, 2000 F31F36
 

Summary:  by AP endonuclease action at abasic sites is likely carried out by a FEN1-like flap endonuclease... . #12;Sekelsky et al. Drosophila DNA Repair Genes F33 tide excision repair endonuclease Rad1/Rad10... - man RAD2 homolog 1 5 - to 3 -exo/endonuclease can efficiently ...

  

Source: Sekelsky, Jeff - Department of Biology, University of North Carolina at Chapel Hill

 

Collection: Biology and Medicine

 
72 Mutation Research 485 (2001) 2336 Nucleotide excision repair "a legacy of creativity"
 

Summary:  /Nbs1). The relative importance of bypass and recombination is determined by the action of p53. We... ; hMre11; p53 1. Introduction The true men of action in our time, those who trans- form the world... single enzyme systems (pyrimidine dimer glycosylase/AP lyase and UV endonuclease) [5]. The two NER sys

  

Source: Karplus, Kevin - Department of Biomolecular Engineering, University of California at Santa Cruz

 

Collection: Biotechnology ; Chemistry

 
73 Role of the Arabidopsis DNA glycosylase lyase ROS1 in active DNA demethylation
 

Summary:  mechanism to achieve this is through the action of 5-methylcytosine DNA glycosylase, which removes... the methylcytosine from DNA leav- ing the deoxyribose intact (10). Local DNA repair then removes the abasic... - and -elimination reactions at the abasic site, after it removes the methylated cytosine base. A weak cleavage

  

Source: Zhu, Jian-Kang - Institute for Integrated Genome Biology, University of California at Riverside

 

Collection: Biotechnology

 
74 Molecular mechanisms of oxidative stress resistance induced by resveratrol: Specific and progressive induction of MnSOD
 

Summary:  of the DNA base excision repair enzymes apurinic/apyrimidinic endonuclease and DNA polymer- ase b. However... and abasic sites, is efficiently removed by the base excision repair (BER) pathway [7], and it is therefore... of the damaged base, leaving an abasic site. This can subsequently be pro- cessed by the enzyme apurinic

  

Source: Stuart, Jeffrey A. - Department of Biological Sciences, Brock University

 

Collection: Biology and Medicine

 
75 Nuclear war: the granzyme A-bomb Judy Lieberman
 

Summary:  protein SET, the DNA bending protein HMG-2, and the base excision repair endonuclease Ape1. The SET... as apoptosis inducing factor [AIF], endonuclease Figure 1 Gzm A Gzm A ? ? ROS Histone H1 DNA Lamins SET... ) and disruption of plasma membrane with prominent blebbing. The mechanism of these actions is unknown

  

Source: Lieberman, Judy - Immune Disease Institute, Harvard University

 

Collection: Biology and Medicine

 
76 The Role of Water in the EcoRIDNA Binding N. Sidorova, D.C. Rau
 

Summary:  , Type II restriction endonucleases are prototypical DNA- binding proteins. In order to avoid... and Molecular Biology,Vol.14 Alfred Pingoud (Ed.) Restriction Endonucleases © Springer-Verlag Berlin Heidelberg... . We focus in this chapter on DNA complexes of the restriction endonuclease EcoRI,as a model system

  

Source: Rau, Don C. - Laboratory of Physical and Structural Biology, National Institute of Child Health and Human Development

 

Collection: Chemistry ; Biology and Medicine

 
77 Fidelity of Dpo4: effect of metal ions, nucleotide selection and pyrophosphorolysis
 

Summary:  epoxide (BPDE) adduct and abasic (Ab) lesions have shown (Ling et al, 2003, 2004a, b), these polymerases... in the active site is near-native. When tested with an abasic or CPD lesions, the effects of Mn2 þ on relaxing... extension. Bypass of (C) a synthetic abasic site and (D) a cis-syn CPD. (E) Terminal nucleotidyl ...

  

Source: Ling, Hong - Department of Biochemistry, University of Alberta

 

Collection: Biology and Medicine

 
78 Supplementary information for The structural basis of RNA-catalyzed RNA polymerization
 

Summary:  Nature Structural & Molecular Biology: doi:10.1038/nsmb.2107 #12;11 the pyridin-2-one and abasic... with EarI endonuclease before use. Nature Structural & Molecular Biology: doi:10.1038/nsmb.2107 #12... where + kabas and - kabas are the apparent first-order self-ligation rate constants for the C47abasic

  

Source: Bartel, David - Whitehead Institute for Biomedical Research & Department of Biology, Massachusetts Institute of Technology (MIT)

 

Collection: Biotechnology ; Biology and Medicine

 
79 ORIGINAL PAPER H. Interthal W.-D. Heyer
 

Summary:  , yeast, and mammals. Mus81p also shares homology with motifs found in the XPF endonuclease superfamily... by HO endonuclease. Double mutant analysis suggests that Rad54p and Mus81p act in one pathway... endonuclease, a homolog of the Saccharomyces cerevisiae Rad1 protein (Aboussekhra et al. 1995). However

  

Source: Heyer, Wolf-Dietrich - Sections of Microbiology & Molecular and Cellular Biology, University of California, Davis

 

Collection: Biology and Medicine

 
80 This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research
 

Summary:  endonucleases, DNA ligase, and DNA polymerases. Furthermore, if abasic sites on oppos- ing DNA strands... action and resistance Daniel J Dwyer1 , Michael A Kohanski1,2 and James J Collins1,2 The alarming spread... species that can actively escape, with mechanistic heterogeneity, the killing action of ...

  

Source: Collins, James J. - Wyss Institute for Biologically Inspired Engineering & Department of Biomedical Engineering, Boston University

 

Collection: Engineering ; Biotechnology


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81 Designs of Autonomous Unidirectional Walking DNA Devices 411 rotation motion [9, 18]. Furthermore, these motions are not autonomously executed but
 

Summary:  in an autonomous unidirectional fashion. Each walking device makes use of alter- nating actions of restriction... enzymes and ligase to achieve unidirectional translational motion. The action of ligase consumes ATP... ;412 P. Yin, A.J. Turberfield, and J.H. Reif Fig. 1. Conceptual endonucleases used in the construction

  

Source: Reif, John H. - Department of Computer Science, Duke University

 

Collection: Computer Technologies and Information Sciences

 
82 Number 23 1995 Los Alamos Science Radiation, Cell Cycle, and Cancer
 

Summary:  the action of one protein to the function of another. Cell Differentiation Cancer is a disease of cells... be in the form of a hormone or a mitogen, into an action that is carried out by the cell. Often, this action

  

Source: Massey, Thomas N. - Department of Physics and Astronomy, Ohio University

 

Collection: Physics

 
83 SURVEY AND SUMMARY Natural and engineered nicking endonucleases
 

Summary:  SURVEY AND SUMMARY Natural and engineered nicking endonucleases --from cleavage mechanism... July 30, 2010; Accepted August 5, 2010 ABSTRACT Restriction endonucleases (REases) are highly specific... the cleavage site) to rare-cutting homing endonucleases (HEases) such as I-HmuI. In addition to these bona fida

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
84 Nucleic Acids Research, 2008, 114 doi:10.1093/nar/gkn1007
 

Summary:  of a bifunctional homing endonuclease and maturase reverses evolutionary degradation Ryo Takeuchi1,2 , Michael Certo... November 26, 2008; Accepted December 1, 2008 ABSTRACT The LAGLIDADG homing endonuclease (LHE) I-AniI has... close homologues of I-AniI. This implies that mutations that have pre- viously reduced the ...

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
85 Association of Dnmt3a and thymine DNA glycosylase links DNA methylation with base-excision repair
 

Summary:  site by the APE DNA endonuclease, which has a higher affinity than TDG to the abasic DNA. Upon... demethylation and de novo methylation by the action of the two de novo methyltransferases, Dnmt3a and Dnmt3b... December 2006 doi:10.1093/nar/gkl1052 #12;ring, generating an abasic residue (18­20). At the ...

  

Source: Tian, Weidong - Institute of Biochemistry and Cell Biology, Shanghai Institute of Biological Sciences

 

Collection: Biology and Medicine

 
86 DNA binding shifts the redox potential of the transcription factor SoxR
 

Summary:  of MutY and Endonuclease III, base excision repair glycosylases that contain a [4Fe-4S] cluster. Initial... of Endonuclease III on bare and DNA- modified graphite demonstrated that binding to DNA shifts the redox potential... indicated by an ``R,'' and the location of abasic sites underlined. Fig. 3. Binding of SoxR to the DNA

  

Source: Dietrich, Lars - Department of Biological Sciences, Columbia University

 

Collection: Biology and Medicine

 
87 Proliferating Cell Nuclear Antigen Promotes Translesion Synthesis by DNA Polymerase *
 

Summary:  bypass synthesis of this model abasic site required the sequential action of Pol and Pol ; however... by Pol on undamaged DNA. PCNA also stimulated translesion synthesis of a model abasic site by Pol... experimental conditions, facile by- pass of the abasic site was also detected by DNA polym- erase or ...

  

Source: Burgers, Peter M. - Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis

 

Collection: Biology and Medicine

 
88 Cell, Vol. 110, 3342, July 12, 2002, Copyright 2002 by Cell Press DEMETER, a DNA Glycosylase Domain Protein,
 

Summary:  is into a full-length DME cDNA (1­6871). To complement the dme nicked 5 to the abasic site by an AP endonuclease... bifunc- tional endonuclease III. Conserved aspartic acid is indicated with an asterisk. Lysine... activity and an AP (apuri- nic or apyrimidinic) endonuclease is responsible forof 3.4 kb of 5 ...

  

Source: Goldberg, Robert B. - Department of Molecular, Cell, and Developmental Biology, University of California at Los Angeles

 

Collection: Biology and Medicine

 
89 A novel function of DNA polymerase f regulated Matthew R Northam1,3
 

Summary:  the catalytic activity of Rev1 normally functions during TLS, particularly during abasic site bypass, Received... inter- actions between the replisome components. These defects usually bring about a spontaneous mutator... of UV-damage, we next investigated bypass of a model abasic site by these enzymes (Figure 5F and G

  

Source: Burgers, Peter M. - Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis

 

Collection: Biology and Medicine

 
90 Molecular Cell Short Article
 

Summary:  Structure-Specific Endonucleases in DNA Repair and Recombination Sabrina L. Andersen,1,5 Daniel T... .06.019 SUMMARY DNA recombination and repair pathways require structure-specific endonucleases to process DNA... MEI-9-ERCC1 endonuclease interacts with the MUS312 protein to produce meiotic crossovers, and that MUS

  

Source: Sekelsky, Jeff - Department of Biology, University of North Carolina at Chapel Hill

 

Collection: Biology and Medicine

 
91 Structure 14, 869880, May 2006 2006 Elsevier Ltd All rights reserved DOI 10.1016/j.str.2006.03.009 The Structure of I-CeuI Homing Endonuclease
 

Summary:  indicated that the progress of the endonuclease re- action is itself asymmetric, with the top strand cleaved... .03.009 The Structure of I-CeuI Homing Endonuclease: Evolving Asymmetric DNA Recognition from a Symmetric Protein... Avenue North Seattle, Washington 98109 Summary Homing endonucleases are highly ...

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
92 Structure, Vol. 9, 12251236, December, 2001, 2001 Elsevier Science Ltd. All rights reserved. PII S0969-2126(01)00685-2 Crystallographic and Modeling Studies
 

Summary:  (Figure 1a). Class 1 enzymes are the simplest, containing an endonuclease domain and a dsRNA binding... domain (dsRBD). Class 2 proteins contain two endonuclease domains and one Jaroslaw Blaszczyk,1 Joseph E... of two endonuclease domains, one dsRBD, and an N-terminal helicase domainBiology Laboratory Center

  

Source: Wlodawer, Alexander - Macromolecular Crystallography Laboratory, National Cancer Institute

 

Collection: Chemistry ; Biology and Medicine

 
93 Enzyme-Activated, Hypoxia-Selective DNA Damage by 3-Amino-2-quinoxalinecarbonitrile 1,4-Di-N-oxide
 

Summary:  excision repair enzyme endo III (for a review of the use of repair endonucleases to study DNA damage, see... and catalyzes strand cleavage at the result- ing abasic site (31-33). We find that treatment of the damaged DNA... of the damaged DNA with the enzyme endo IV, which catalyzes strand cleavage at abasic sites and oxidized ...

  

Source: Gates, Kent. S. - Departments of Chemistry & Biochemistry, University of Missouri-Columbia

 

Collection: Chemistry

 
94 Single cell trapping and DNA damage analysis using microwell arrays
 

Summary:  of a critical DNA repair enzyme, human AP endonuclease. This platform enables high throughput assessment... be detected when converted to abasic sites or single strand breaks with the addition of purified DNA repair... for different repair pathways. Small Molecule Inhibitors of Human AP Endonuclease. DNA dama- ging agents

  

Source: Bhatia, Sangeeta - Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology (MIT)

 

Collection: Biotechnology ; Biology and Medicine

 
95 Single cell trapping and DNA damage analysis using microwell arrays
 

Summary:  of a critical DNA repair enzyme, human AP endonuclease. This platform enables high throughput assessment... be detected when converted to abasic sites or single strand breaks with the addition of purified DNA repair... Inhibitors of Human AP Endonuclease. DNA dama- ging agents are the frontline treatment for most cancers

  

Source: Engelward, Bevin - Division of Bioengineering, Massachusetts Institute of Technology (MIT)

 

Collection: Biology and Medicine

 
96 Brandt F.Eichman1 , Eyleen J.O'Rourke1,2
 

Summary:  to crystal and NMR structures of the unliganded enzymes and com- plexes with abasic DNA inhibitors. Presented... - actions with purine or methyl substituent atoms, but rather by stacking the base between two aromatic side... coli endonuclease III (EndoIII) (Thayer et al., 1995), and it was soon discovered that the overall

  

Source: Eichman, Brandt F. - Department of Biological Sciences, Vanderbilt University

 

Collection: Biology and Medicine

 
97 INTRINSIC PROPERTIES OF THE TWO REPLICATIVE DNA POLYMERASES OF PYROCOCCUS ABYSSI IN REPLICATING ABASIC SITES: POSSIBLE ROLE IN DNA
 

Summary:  ABASIC SITES: POSSIBLE ROLE IN DNA DAMAGE TOLERANCE ? 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20... , France. Running Tittle : DNA synthesis by Pabpols of DNA containing abasic sites * Corresponding author... 39 40 41 Spontaneous and induced abasic sites in hyperthermophiles DNA have long been suspected

  

Source: Ecole Polytechnique, Centre de mathématiques

 

Collection: Mathematics

 
98 When a helicase is not a helicase: dsDNA tracking by the motor protein EcoR124I
 

Summary:  and endonuclease activities (Szczelkun et al, 1996; Firman and Szczelkun, 2000; Seidel et al, 2004, 2005; Mc... ­50 strand; and two abasic groups in 30 ­50 strand introduced by annealing a modified oligonucleotide... (Janscak et al, 1999; also, see below). Therefore, ICLs may actually induce endonuclease activity through

  

Source: Seidel, Ralf - Kavli Institute of Nanoscience, Technische Universiteit Delft

 

Collection: Biotechnology

 
99 2000 Macmillan Magazines Ltd letters to nature
 

Summary:  .nature.com reaction of the major human abasic endonuclease: new insights from EDTA-resistant incision of acyclic... abasic site analogs and site-directed mutagenesis. J. Mol. Biol. 290, 447±457 (1999). 13. Izumi, T. et al... . Intragenic suppression of an active site mutation in the human apurinic/apyrimidinic ...

  

Source: Engelman, Donald M.- Department of Molecular Biophysics and Biochemistry, Yale University

 

Collection: Biology and Medicine

 
100 Noncovalent Spin Labeling DOI: 10.1002/anie.201002637
 

Summary:  ,7] The strategy for noncovalent labeling was to disconnect the glycosidic bond of C¸ to give an abasic site (F... ) and the free spin-labeled base ç (Figure 1). The spin label would bind in the abasic site through receptor... containing an abasic site with ç, a slow-moving component appears in the EPR spectrum (shown by arrows

  

Source: Sigurdsson, Snorri Thor - Chemistry Division, Science Institute, University of Iceland.

 

Collection: Chemistry


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