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Sample search results for: abasic endonuclease activity

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1 Oligonucleotides with Bistranded Abasic Sites Interfere with Substrate Binding and Catalysis by Human Apurinic/Apyrimidinic Endonuclease
 

Summary:  -27). The redox activity appears to be unrelated to the ability to cleave abasic sites (27). Because this enzyme... substrate con- centration and the halved specific activity. Also, the position of the second abasic site... nucleotides upstream of the abasic site which are required for ...

  

Source: Strauss, Phyllis R. - Department of Biology, Northeastern University

 

Collection: Biology and Medicine

 
2 Human Apurinic/Apyrimidinic Endonuclease Is Processive, Daniel C. Carey and Phyllis R. Strauss*
 

Summary:  Human Apurinic/Apyrimidinic Endonuclease Is Processive, Daniel C. Carey and Phyllis R. Strauss... Vised Manuscript ReceiVed October 7, 1999 ABSTRACT: Apurinic/apyrimidinic endonuclease (AP endo) is believed... abasic sites in the base excision repair pathway. AP endo makes a single nick 5 to an abasic site

  

Source: Strauss, Phyllis R. - Department of Biology, Northeastern University

 

Collection: Biology and Medicine

 
3 Submitted to J. Biol. Chem. Draft of July 13, 2001
 

Summary:  /apyrimidinic endonuclease; ASR, abasic site repair, bp, base pair; ds, double-stranded; ESR, electron spin resonance; HDP... and nicking activity is seen; however, if the second abasic site is positioned 5 to the first, enzymatic... activity is greatly decreased, especially if the second ...

  

Source: Strauss, Phyllis R. - Department of Biology, Northeastern University

 

Collection: Biology and Medicine

 
4 Abasic Site Repair in Higher Eukaryotes Phyllis R. Strauss and Noreen E. O'Regan
 

Summary:  activity, as occurs when the abasic site is reduced (109) (see below). In other pol -deficient cells, long... is cleavage of the abasic site on the 5 side of the phosphodeoxyribose in ds DNA (58,82,231). This activity... containing an abasic site (HDP) acts as a powerful inhibitor of the ...

  

Source: Strauss, Phyllis R. - Department of Biology, Northeastern University

 

Collection: Biology and Medicine

 
5 Substrate Binding by Human Apurinic/Apyrimidinic Endonuclease Indicates a Briggs-Haldane Mechanism*
 

Summary:  Apurinic/apyrimidinic endonuclease (AP endo) makes a single nick 5 to a DNA abasic site. We have character... on the complementary strand (12, 13). The endonuclease activity of AP endo has been well charac- terized for its... was 30 nM (Fig. 3B). Thus the enhancement of AP endo activity ...

  

Source: Strauss, Phyllis R. - Department of Biology, Northeastern University

 

Collection: Biology and Medicine

 
6 Notes & Tips A nonisotopic assay for unambiguous assignment of DNA
 

Summary:  -scission activity (i.e., DNA glycosylases/AP-lyases), monofunctional DNA glycosylases do not cleave the DNA backbone... but leave be- hind an abasic or apurinic/apyrimidinic (AP)1 site in DNA [4]. Thus, for the analysis... strand scission adjacent to the abasic site. The lack of current DNA glycosylase assays to concur- rently

  

Source: Boston University, Center for Advanced Biotechnology

 

Collection: Biotechnology

 
7 DNA Repair 3 (2004) 14471455 Novel role of tyrosine in catalysis by human AP endonuclease 1
 

Summary:  for cleavage of the abasic site. 3.6. Imidazole enhances activity of WT enzyme markedly, enhances Y171A... involve attack on the 5 -phosphate of the abasic site by an activated water molecule in analogy... human abasic endonuclease: new insights from EDTA-resistant ...

  

Source: Strauss, Phyllis R. - Department of Biology, Northeastern University

 

Collection: Biology and Medicine

 
8 Domain Mapping of Human Apurinic/Apyrimidinic Endonuclease STRUCTURAL AND FUNCTIONAL EVIDENCE FOR A DISORDERED AMINO TERMINUS AND A TIGHT
 

Summary:  directly in the redox activity. Apurinic/apyrimidinic endonuclease (AP endo)1 is a critical enzyme... then would an abasic site interact with the active site residues of AP endo? Even in the absence of enzyme... Domain Mapping of Human Apurinic/Apyrimidinic Endonuclease STRUCTURAL AND ...

  

Source: Strauss, Phyllis R. - Department of Biology, Northeastern University

 

Collection: Biology and Medicine

 
9 Clustered DNA damages induced in isolated DNA and in human cells by low doses of ionizing radiation
 

Summary:  (48­51) E. coli Nfo protein (endonuclease IV) Abasic sites Several types of abasic sites, including... , abasic sites, or oxidized bases) on opposing strands--are suspects as critical lesions producing lethal... from human cells. DNA is treated with an endonuclease that induces a ...

  

Source: Brookhaven National Laboratory, Environmental Chemistry Division, Department of Applied Science

 

Collection: Environmental Sciences and Ecology

 
10 Effect of Protein Binding on Ultrafast DNA Dynamics: Characterization of a DNA:APE1 Complex
 

Summary:  that lacks endonuclease activity, but retains the ability to bind to abasic sites (29,30). In a second set... . Demple. 1995. Incision activity of human apurinic endonuclease (APE) at abasic site analogs in DNA. J... by testing its biological ...

  

Source: Coleman, Robert S. - Department of Chemistry, Ohio State University

 

Collection: Chemistry

 
11 Single-Turnover Analysis of Mutant Human Apurinic/Apyrimidinic Endonuclease, Julie A. Lucas, Yuji Masuda,|, Richard A. O. Bennett,| Nathaniel S. Strauss,,@ and Phyllis R. Strauss*,
 

Summary:  and cleavage of abasic site-containing DNA. Apurinic/apyrimidinic endonuclease (AP endo) is a human enzyme... activity on the part of D283A is consistent with the ability of this mutant to bind an abasic site... Single-Turnover Analysis of Mutant Human Apurinic/Apyrimidinic Endonuclease, ...

  

Source: Strauss, Phyllis R. - Department of Biology, Northeastern University

 

Collection: Biology and Medicine

 
12 Mitochondrial and nuclear DNA base excision repair are affected differently by caloric restriction1
 

Summary:  ) and processing of the resulting abasic site by AP endonuclease. These activities were measured as percent... in AP endonuclease and polymerase activities in mitochondria The first steps in the repair of uracil... ). However, kidney UNG activity was 40% higher in CR ...

  

Source: Stuart, Jeffrey A. - Department of Biological Sciences, Brock University

 

Collection: Biology and Medicine

 
13 Multiple Cleavage Activities of Endonuclease V from Thermotoga maritima: Recognition and Strand Nicking Mechanism
 

Summary:  Multiple Cleavage Activities of Endonuclease V from Thermotoga maritima: Recognition and Strand... the uracil base and with AP endonucleases and other enzymes to repair the subsequent abasic site (4, 5... ), and small insertions/deletions (15). E. coli endoV is also a urea endonuclease but ...

  

Source: Barany, Francis - Biochemistry and Structural Biology Program, Weill Cornell Graduate School of Medical Sciences

 

Collection: Biology and Medicine

 
14 DNA base excision repair activities and pathway function in mitochondrial and cellular lysates from
 

Summary:  . While nuclear BER protein levels and activities were generally not altered in r0 cells, AP endonuclease... of r0 cells, as exposure of cells to ROS rapidly stimulated increases in AP endonuclease activities... activities. MATERIALS AND METHODS Puri®ed proteins Human ...

  

Source: Stuart, Jeffrey A. - Department of Biological Sciences, Brock University

 

Collection: Biology and Medicine

 
15 Mutational Analysis of Endonuclease V from Thermotoga maritima Jianmin Huang,, Jing Lu,,,| Francis Barany, and Weiguo Cao*,,|
 

Summary:  , endonuclease V is active toward AP site, uracil, and mismatches (2, 4-6). With Mn2+ as the metal cofactor... enzyme excess over substrate, D43A and D110A did not show any DNA endonuclease activity for cleaving... of enzyme)-1 ]. D43A, E89A, and D110A did not show detectable inosine ...

  

Source: Barany, Francis - Biochemistry and Structural Biology Program, Weill Cornell Graduate School of Medical Sciences

 

Collection: Biology and Medicine

 
16 3-Methyladenine DNA glycosylases: structure,
 

Summary:  an alternative way to process abasic sites. Some DNA glycosylases have an associated -lyase activity... -glycosylic bond between the base and the deoxyribose. 5'-AP endonuclease activity cleaves the deoxyribose... after AP endonuclease activity represents the ...

  

Source: Lau, Albert Y. - Department of Biophysics and Biophysical Chemistry, Johns Hopkins University

 

Collection: Biology and Medicine

 
17 Ubiquitinated proliferating cell nuclear antigen activates translesion DNA polymerases and REV1
 

Summary:  , the flap endonuclease FEN1, and DNA ligase I. However, whereas the activity of DNA polymerase remains... is a deoxycytidyl transferase that shows the highest catalytic activity opposite template guanines and abasic sites... was responsive to the modified clamp. Rev1 is primarily active ...

  

Source: Burgers, Peter M. - Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis

 

Collection: Biology and Medicine

 
18 Oxidative DNA Base Damage by the Antitumor Agent 3-Amino-1,2,4-benzotriazine 1,4-Dioxide (Tirapazamine)
 

Summary:  it would be informative to compare the endonuclease fingerprint of enzymati- cally-activated tirapazamine... Scheme 2. Figure 2. Endonuclease fingerprint for tirapazamine-mediated DNA damage (activated by NADPH... --Tirapazamine is a bioreductively activated DNA-damaging agent that ...

  

Source: Gates, Kent. S. - Departments of Chemistry & Biochemistry, University of Missouri-Columbia

 

Collection: Chemistry

 
19 This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research
 

Summary:  III, Characterization of abasic endonuclease activity of human Ape1 on alternative substrates, as well... ­568. [20] D.M. Wilson III, Ape1 abasic endonuclease activity is regulated by magnesium and potassium... A, modulate the single-stranded ...

  

Source: Rader, Stephen - Chemistry Program, University of Northern British Columbia

 

Collection: Biology and Medicine ; Chemistry

 
20 Effect of lesions on the dynamics of DNA on the picosecond and nanosecond timescales using a
 

Summary:  of the abasic site is important in its recognition by the endonuclease (46,47). The second lesion studied... is the ¯ipping of the abasic sugar out of the helix and the concomitant intrusion of water into the interior... DNA: an abasic site and a helix terminus. Because these comparisons are looking for ...

  

Source: Coleman, Robert S. - Department of Chemistry, Ohio State University

 

Collection: Chemistry


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21 Molecular Cell, Vol. 20, 367375, November 11, 2005, Copyright 2005 by Elsevier Inc. DOI 10.1016/j.molcel.2005.09.018 MRE11/RAD50 Cleaves DNA
 

Summary:  generated by successive action of AID and UNG2, be- cause it is the major abasic endonuclease active in base... exonuclease activity by targeted mutagenesis. Unlike APE1, the major AP-endonuclease, which cleaves AP sites... biochemical identifica- tion of other ...

  

Source: Maizels, Nancy - Department of Biochemistry, University of Washington at Seattle

 

Collection: Biology and Medicine

 
22 MOLECULAR AND CELLULAR BIOLOGY, Sept. 2002, p. 64806486 Vol. 22, No. 18 0270-7306/02/$04.00 0 DOI: 10.1128/MCB.22.18.64806486.2002
 

Summary:  endonuclease activity and functions in the removal of abasic sites from DNA. Here, we provide evidence... activity of human apurinic endonuclease (Ape) at abasic site analogs in DNA. J. Biol. Chem. 270... , the major AP endonuclease of ...

  

Source: Burgers, Peter M. - Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis

 

Collection: Biology and Medicine

 
23 University of Maryland Baltimore Graduate School Announcement of Doctoral Dissertation Defense
 

Summary:  of these damaged bases result in the formation of an abasic or AP site, allowing the next enzyme in the pathway... , Apurinic/Apyrimidinic Endonuclease 1 (APE1), to hydrolytically cleave the DNA backbone 5' to the AP site... found to bind at the repair active site. We have also investigated the structure of APE1 using X

  

Source: Weber, David J. - Department of Biochemistry and Molecular Biology, University of Maryland-Baltimore

 

Collection: Biology and Medicine

 
24 Molecular Cell, Vol. 13, 751762, March 12, 2004, Copyright 2004 by Cell Press Snapshots of Replication through
 

Summary:  - abasic lesion at various desired positions relative to the Dpo4 active site, we used either an inactive 3... ), or deletion ( 1) products. the active site, as in Ab-1 and Ab-2A, is probably only abasic site... of Replication through an Abasic Lesion: Structural Basis for Base ...

  

Source: Ling, Hong - Department of Biochemistry, University of Alberta

 

Collection: Biology and Medicine

 
25 Base Excision and DNA Binding Activities of Human Alkyladenine DNA Glycosylase Are Sensitive to the Base Paired with a Lesion*
 

Summary:  Base Excision and DNA Binding Activities of Human Alkyladenine DNA Glycosylase Are Sensitive... specificity of human alkyladenine DNA glycosy- lase, kinetics of excision and DNA binding activities were... affinity of human alkylad- enine DNA glycosylase to a DNA product containing an abasic site was similar

  

Source: Lau, Albert Y. - Department of Biophysics and Biophysical Chemistry, Johns Hopkins University

 

Collection: Biology and Medicine

 
26 Cell, Vol. 95, 249258, October 16, 1998, Copyright 1998 by Cell Press Crystal Structure of a Human Alkylbase-DNA
 

Summary:  nucleotide to flip into the en- sylase reaction, a pyrrolidine abasic nucleotide, which zyme active site... ) the active site strongly suggests that the bound water complexed to DNA containing a pyrrolidine abasic... of the flipped out pyrrolidine abasic nucleotide. Residues of the ...

  

Source: Lau, Albert Y. - Department of Biophysics and Biophysical Chemistry, Johns Hopkins University

 

Collection: Biology and Medicine

 
27 DNA Repair 1 (2002) 645659 The S. cerevisiae Mag1 3-methyladenine DNA glycosylase
 

Summary:  is then cleaved 5 to the abasic sugar by the major AP endonuclease, Apn1, which is responsi- ble for >95... % of the AP endonuclease activity in S. cerevisiae [21]. The 3 hydroxyl group created by Apn1 is then extended... known. Uncleaved abasic sites inhibit DNA replication [49], and ...

  

Source: Engelward, Bevin - Division of Bioengineering, Massachusetts Institute of Technology (MIT)

 

Collection: Biology and Medicine

 
28 Noncovalent Spin Labeling DOI: 10.1002/anie.201002637
 

Summary:  ,7] The strategy for noncovalent labeling was to disconnect the glycosidic bond of C¸ to give an abasic site (F... ) and the free spin-labeled base ç (Figure 1). The spin label would bind in the abasic site through receptor... in the presence of a catalytic amount of iodine to obtain compound 2 (Scheme 1).[9] Activation of 2 by ...

  

Source: Sigurdsson, Snorri Thor - Chemistry Division, Science Institute, University of Iceland.

 

Collection: Chemistry

 
29 JOURNAL OF BACTERIOLOGY, July 2002, p. 35013507 Vol. 184, No. 13 0021-9193/02/$04.00 0 DOI: 10.1128/JB.184.13.35013507.2002
 

Summary:  and the deoxyribose, resulting in an abasic (AP) site. Subsequently, AP endonucleases incise the DNA backbone... deficient in AP endonuclease activity (double mutant in exonuclease III and endonuclease IV: xth nfo... glycosylases are active in the production of AP ...

  

Source: Engelward, Bevin - Division of Bioengineering, Massachusetts Institute of Technology (MIT)

 

Collection: Biology and Medicine

 
30 MicroCommentary Social networking between mobile introns and their
 

Summary:  and splicing of the intron, and also to participate in folding and activity of the endonuclease. Whereas... of endonuclease activity, deletion of the intron and possibly reinvasion by a related endonuclease (Goddard... . This activity is often coupled to the efficient ...

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
31 DNA damage recognition and repair by 3-methyladenine DNA glycosylase I (TAG)
 

Summary:  to other glycosylase-DNA structures, the abasic ri- bose is not flipped into the TAG active site... between the abasic moiety and TAG's active site in the product complex argue that the 3mA glycosylic bond... glycosylase-DNA structures, the abasic ribose in the TAG complex is not fully ...

  

Source: Eichman, Brandt F. - Department of Biological Sciences, Vanderbilt University

 

Collection: Biology and Medicine

 
32 Characterization of DNA Damage Induced by a Natural Product Antitumor Antibiotic Leinamycin in Human Cancer Cells
 

Summary:  ., and Wilson, D. M., III. (2008) Characterization of abasic endonuclease activity of human Ape1 on alternative... , 1­12. (24) Wilson, D. M., III., and Barsky, D. (2001) The major human abasic endonuclease: Formation... is a structurally novel Streptomyces-derived natural product that ...

  

Source: Gates, Kent. S. - Departments of Chemistry & Biochemistry, University of Missouri-Columbia

 

Collection: Chemistry

 
33 Genome Biology 2004, 5:211 commentreviewsreportsdepositedresearchinteractionsinformationrefereedresearch
 

Summary:  endonucleases to nick the abasic site so that new DNA synthe- sis can recreate the duplex [6]. Reasoning... @u.washington.edu Abstract Activation-induced deaminase (AID) initiates switch recombination and somatic hypermutation... of immunoglobulin genes in activated B cells. Compelling evidence now shows that ...

  

Source: Maizels, Nancy - Department of Biochemistry, University of Washington at Seattle

 

Collection: Biology and Medicine

 
34 Z .Mutation Research 460 2000 201210 www.elsevier.comrlocaterdnarepair
 

Summary:  out 1-azaribose abasic nucleotide in the active site of AlkA shows that the 3-methyl- adenine base... , Modulation of the DNA scan- ning activity of the Micrococcus luteus UV endonuclease, J. Z .Biol. Chem. 264... of the active site, and that the enzyme activates a ...

  

Source: Lau, Albert Y. - Department of Biophysics and Biophysical Chemistry, Johns Hopkins University

 

Collection: Biology and Medicine

 
35 DEMETER DNA Glycosylase Establishes MEDEA Polycomb Gene Self-Imprinting
 

Summary:  -glycosylic bond, creating an abasic site, whereas the lyase activity nicks the DNA. An AP endonuclease generates... ) (Figure 5B, lane 3). By contrast, there is significantly less inhibition of DME activity when the abasic... in crosses between Ler and Cvi and Ler Figure 5. Inhibition of ...

  

Source: Goldberg, Robert B. - Department of Molecular, Cell, and Developmental Biology, University of California at Los Angeles

 

Collection: Biology and Medicine

 
36 Localization of mitochondrial DNA base excision repair to an inner membrane-associated
 

Summary:  of the abasic site following uracil removal even in fractions deficient in AP endonuclease activity (r0... centri- fuged, dried, suspended in 10 ml of formamide loading dye. AP endonuclease activity AP... endonuclease activities of mitochondria ...

  

Source: Stuart, Jeffrey A. - Department of Biological Sciences, Brock University

 

Collection: Biology and Medicine

 
37 Direct Real Time Observation of Base Flipping by the EcoRI DNA Methyltransferase*
 

Summary:  active site. The ener- getics and kinetics of base flipping by the EcoRI DNA methyltransferase were... for the binding of the methyltransferase to DNA containing abasic sites or base analogs incorpo- rated... the correct assembly of active site residues frequently demands the insertion of protein side chains

  

Source: Reich, Norbert O. - Department of Chemistry and Biochemistry, University of California at Santa Barbara

 

Collection: Chemistry ; Biology and Medicine

 
38 JOURNAL OF BACTERIOLOGY, 0021-9193/01/$04.00 0 DOI: 10.1128/JB.183.1.131138.2001
 

Summary:  /apurinic (AP) endonucleases at the abasic site. This results in a 3 -OH terminus, which can act as a primer... endonuclease activity were very sensitive to NO toxicity, sug- gesting the formation of AP sites (generated... ). WT, wild type. VOL. 183, 2001 RECOMBINATIONAL REPAIR OF NO DAMAGE 133 #12;AP ...

  

Source: Engelward, Bevin - Division of Bioengineering, Massachusetts Institute of Technology (MIT)

 

Collection: Biology and Medicine

 
39 Homologous recombination prevents methylation-induced toxicity in Escherichia coli
 

Summary:  sites and then AP endonuclease activity cleaves the DNA backbone. Second, abasic sites that are produced... resulting from AP-endonuclease action. INTRODUCTION Multiple DNA repair systems specific for alkylation... A and constitutive Tag glycosylases constitute a second mechanism to remove principally N3-meA, ...

  

Source: Engelward, Bevin - Division of Bioengineering, Massachusetts Institute of Technology (MIT)

 

Collection: Biology and Medicine

 
40 BioMed Central Page 1 of 6
 

Summary:  the DNA leaving an abasic site where the strand is nicked at both the 3' and 5' ends [6]. The abasic site... Y [9,10], which excises the mispaired adenine A leaving an abasic site. The abasic site opposite... into the active site cleft during their search for excision targets [16]. ...

  

Source: Lunds Universitet, - Computational Biology and Biological Physics Group

 

Collection: Physics ; Biology and Medicine


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41 Post-doctoral Fellowships University of Washington
 

Summary:  2010 -2011 Post-doctoral Fellowships University of Washington Seattle, WA Homing endonuclease... design/engineering Targeted disease gene repair/Functional genomics Background: Homing endonucleases (HE... 's) are highly site-specific DNA endonucleases that catalyze the lateral transfer of parasitic DNA elements

  

Source: Monnat, Ray - Department of Genome Sciences, University of Washington at Seattle

 

Collection: Biology and Medicine

 
42 Charge Transport in DNA Oligonucleotides with Various Base-Pairing Patterns Irena Kratochvilova,*,
 

Summary:  . Structurally and thermally much less stable abasic duplexes have still lower conductivity but not markedly... , four, and five base pairs, respectively, eliminated from their central parts (ABA- SIC3, ABASIC4... , and ABASIC5). All of the duplexes were prepared from single-stranded oligonucleotides synthesized in an ABI

  

Source: Kužel, Petr - Institute of Physics, Academy of Sciences of the Czech Republic

 

Collection: Physics

 
43 Homingendonucleasestructureandfunction Barry L. Stoddard
 

Summary:  endonucleases 30 7. Intron splicing (maturase) activity by homing endonucleases 31 7.1 Distribution... -strand break. Transfer of group I introns and inteins is initiated solely by the endonuclease activity... the endonuclease activity of the ...

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
44 Advances in Engineering Homing Endonucleases for GeneTargeting
 

Summary:  of homing endonuclease design and selection, and aim to optimize the specificity and activity... LAGLIDADG homing endonuclease (I-AniI) bound to its 19 basepair cognate target site. The active sites... transfected eukaryotic cells overexpress active endonuclease ...

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
45 Cell, Vol. 110, 3342, July 12, 2002, Copyright 2002 by Cell Press DEMETER, a DNA Glycosylase Domain Protein,
 

Summary:  activity and an AP (apuri- nic or apyrimidinic) endonuclease is responsible forof 3.4 kb of 5 -flanking DME... is into a full-length DME cDNA (1­6871). To complement the dme nicked 5 to the abasic site by an AP endonuclease... , a DNA glyco- for subsequent embryo and endosperm development sylase ...

  

Source: Goldberg, Robert B. - Department of Molecular, Cell, and Developmental Biology, University of California at Los Angeles

 

Collection: Biology and Medicine

 
46 Mitochondrial DNA maintenance and bioenergetics Jeffrey A. Stuart , Melanie F. Brown
 

Summary:  the first step of this pathway, a DNA glycosylase removes the damaged base leaving an abasic site. AP-endonuclease... mitochondria. The apurinic/ apyrimidinic endonuclease Apn1 is the major AP endonuclease activity in yeast... that contains inner membrane proteins [119]. With the ...

  

Source: Stuart, Jeffrey A. - Department of Biological Sciences, Brock University

 

Collection: Biology and Medicine

 
47 CMLS, Cell. Mol. Life Sci. 55 (1999) 13041326 1420-682X/99/10130423 $ 1.50+0.20/0
 

Summary:  /M') and carry both homing endonuclease and maturase activity. (B) Group II intron-encoded proteins are generally... activities of endonuclease and reverse transcriptase domains. The protein binds the excised group II intron... in this site block endonuclease ...

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
48 Holliday junctions in the eukaryotic nucleus: resolution in sight?
 

Summary:  ]. There is no evidence that these nuclear-encoded enzymes have a nuclear function. Endonuclease activity capable of HJ... of the nuclease domain of restriction endonucleases, including the conserved signa- ture motif of the active site... ­6549 29 deLaat, W.L. et al. (1998) DNA structural elements required ...

  

Source: Heyer, Wolf-Dietrich - Sections of Microbiology & Molecular and Cellular Biology, University of California, Davis

 

Collection: Biology and Medicine

 
49 Correlation of mitochondrial superoxide dismutase and DNA polymerase b in mammalian dermal fibroblasts with species maximal lifespan
 

Summary:  in parallel to APE activity assays: the addition of control oligonucleotide (no abasic site analogue); no cell... .05 as in Table 3). Fig. 4. AP endonuclease activity does not correlate with species lifespan. (A) APE activity... .00643 8.71 Æ 1.73 8.07 Æ 1.46 APE = AP ...

  

Source: Stuart, Jeffrey A. - Department of Biological Sciences, Brock University

 

Collection: Biology and Medicine

 
50 An Overview of Chemical Processes That Damage Cellular DNA: Spontaneous Hydrolysis, Alkylation, and Reactions with Radicals
 

Summary:  Backbone 1749 2.4. Properties of Abasic Sites Arising from Depurination 1749 3. Overview: Common Reactions... and 5-methylcytosine accelerate deamination (34-41). Activation- induced cytidine deaminases catalyze... ). The oxocarbenium ion undergoes subsequent hydrolysis to yield an abasic site 7 (often referred to as an apurinic

  

Source: Gates, Kent. S. - Departments of Chemistry & Biochemistry, University of Missouri-Columbia

 

Collection: Chemistry

 
51 DNA Binding and Cleavage by the HNH Homing Endonuclease I-HmuI
 

Summary:  -binding surface found in the I-PpoI homing endonuclease and an associated HNH/N active site found in the bacterial... , and L84M) were incorporated. The resulting mutated SeMet construct was assayed for endonuclease activity... Endonuclease #12;Figure 3. The HNH motif and ...

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
52 Nucleic Acids Research, 2009, 114 doi:10.1093/nar/gkp095
 

Summary:  , 2009 7 #12;Vsr endonucleases have a type II restriction enzyme topology, but their active sites... , possibly relating to the difference in activity between repair and homing endonucleases. No significant... arrangement involving new split inteins and a new homing endonuclease family ...

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
53 Single cell trapping and DNA damage analysis using microwell arrays
 

Summary:  endonuclease activity of APE1 could thus be extremely valuable in combination therapies for treating cancer... endonuclease activity (42, 43, 44). Cytotoxicity of these molecules has been evaluated by clonogenic survival... /ref-1 endonuclease activity in pancreatic ...

  

Source: Bhatia, Sangeeta - Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology (MIT)

 

Collection: Biotechnology ; Biology and Medicine

 
54 Single cell trapping and DNA damage analysis using microwell arrays
 

Summary:  ­41). Small molecules that inhibit the AP endonuclease activity of APE1 could thus be extremely valuable... - tential inhibitors of APE1's AP endonuclease activity (42, 43, 44). Cytotoxicity of these molecules has... , Skalski V, Hedley DW (2004) Effects of gemcitabine on APE/ref-1 ...

  

Source: Engelward, Bevin - Division of Bioengineering, Massachusetts Institute of Technology (MIT)

 

Collection: Biology and Medicine

 
55 Formation of DNA-Protein Cross-Links Between -Hydroxypropanodeoxyguanosine and EcoRI
 

Summary:  of an abasic site cross-linked to the catalytically active N-terminal threonine of T4 PDG and the imine... , stabilization of the cross-link by reduction of the Schiff base linkage resulted in loss of enzyme activity... endonucleases represents a useful model system with which to evaluate the impact of DNA ...

  

Source: Marnett, Lawrence J. - Department of Chemistry, Vanderbilt University

 

Collection: Biology and Medicine ; Chemistry

 
56 ANRV260-GE39-03 ARI 21 June 2005 22:14 Immunoglobulin Gene
 

Summary:  , abasic endonucleases nick the phosphodi- ester backbone at the abasic site, DNA poly- merase synthesizes... containing abasic sites, including abasic endonucleases like APE1, which cleave the phosphodiester back- bone... -fidelity DNA synthesis (translesion ...

  

Source: Maizels, Nancy - Department of Biochemistry, University of Washington at Seattle

 

Collection: Biology and Medicine

 
57 DNA Distortion and Specificity in a Sequence-Specific Endonuclease
 

Summary:  artifacts of the biochemical and structural studies. Restriction endonucleases typically contain an active... - cleavage rates.24,26­28 These studies suggest that the optimal endonuclease activity depends... DNA Distortion and Specificity in a Sequence-Specific Endonuclease Andrea C. ...

  

Source: Horton, Nancy C. - Department of Chemistry and Biochemistry, University of Arizona

 

Collection: Biology and Medicine ; Chemistry

 
58 The conformational modification of serpins transforms Leukocyte Elastase Inhibitor into an endonuclease involved in apoptosis.
 

Summary:  . The endonuclease responsible for DNA degradation in caspase- dependent apoptosis is Caspase Activated DNase (CAD... ). In caspase-independent apoptosis, different endonucleases may be activated according to the cell line... -protease activity and leads to a conformational modification ...

  

Source: Ecole Polytechnique, Centre de mathématiques

 

Collection: Mathematics

 
59 Crystallization and Preliminary X-ray Studies of I-CreI: A Group I Intron-Encoded Endonuclease
 

Summary:  ``inteins.'' The catalytic activity of these endonucleases leads to their self-propagation by a targeted... endonuclease activity and invivo mobility. EMBO J. 10:3495­3501, 1991. 9. Thompson, A.J., Yuan, X., Kudlicki, W... activities of PI-SceI endonuclease. J. ...

  

Source: Monnat, Ray - Department of Genome Sciences, University of Washington at Seattle

 

Collection: Biology and Medicine

 
60 Site-Directed Spin-Labeling of Nucleic Acids by Click Chemistry: Detection of Abasic Sites in Duplex DNA by EPR
 

Summary:  Site-Directed Spin-Labeling of Nucleic Acids by Click Chemistry: Detection of Abasic Sites... that can detect and identify local structural deformations in duplex DNA, in particular abasic sites... , for the first time, abasic sites in duplex DNA by X-band CW-EPR spectroscopy and give information about other

  

Source: Sigurdsson, Snorri Thor - Chemistry Division, Science Institute, University of Iceland.

 

Collection: Chemistry


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61 A Ubiquitin-binding Motif in the Translesion DNA Polymerase Rev1 Mediates Its Essential Functional Interaction with
 

Summary:  sensitivity of Rev1 activity during abasic site bypass. A, sub- strate was V9-AP2. Directly downstream... polymerase activity (47). Consist- ent with this, the activity of Rev1-1 at an abasic site was only about 20... restrictive active site to promote proper ...

  

Source: Burgers, Peter M. - Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis

 

Collection: Biology and Medicine

 
62 2000 Macmillan Magazines Ltd letters to nature
 

Summary:  .nature.com reaction of the major human abasic endonuclease: new insights from EDTA-resistant incision of acyclic... . Intragenic suppression of an active site mutation in the human apurinic/apyrimidinic endonuclease. J. Mol... abasic site analogs and site-directed mutagenesis. J. Mol. ...

  

Source: Engelman, Donald M.- Department of Molecular Biophysics and Biochemistry, Yale University

 

Collection: Biology and Medicine

 
63 Chemical Properties of the Leinamycin-Guanine Adduct Tony Nooner, Sanjay Dutta, and Kent S. Gates*
 

Summary:  . Res. 461, 83-108. (35) Wilson, D. M., III, and Barsky, D. (2001) The major human abasic endonuclease... at several temperatures, and the activation parameters were calculated from the data. The energy... of activation (Ea) for this reaction is 24.6 kcal/mol, and the Arrhenius A value is 1.2 × 1013 s-1. ...

  

Source: Gates, Kent. S. - Departments of Chemistry & Biochemistry, University of Missouri-Columbia

 

Collection: Chemistry

 
64 Molecular basis for discriminating between normal and damaged bases by the human alkyladenine
 

Summary:  be distinguished from normal DNA bases in the enzyme active site. Mutational analyses of residues contacting... enzymatic activities tem- plated by DNA. Hypoxanthine is an abundant deaminated base, and it too corrupts... , and the resulting abasic nucleotide is excised and replaced with a normal nucleotide by the sequential ...

  

Source: Lau, Albert Y. - Department of Biophysics and Biophysical Chemistry, Johns Hopkins University

 

Collection: Biology and Medicine

 
65 Chemistry & Biology, Vol. 12, 357369, March, 2005, 2005 Elsevier Ltd All rights reserved. DOI 10.1016/j.chembiol.2004.12.011 Delineation of the Chemical Pathways Underlying
 

Summary:  susceptibility to loss of heterozygosity pro- by activated macrophages, along with oxygen radicals vides... that it induces. NO· reacts with oxygen and su- active oxygen and nitrogen species is thought to cause peroxide... of guanine leads to 8-oxoguanine and its secondary ox- idation products, 8-nitroguanine, as well as abasic

  

Source: Engelward, Bevin - Division of Bioengineering, Massachusetts Institute of Technology (MIT)

 

Collection: Biology and Medicine

 
66 Small interfering RNAs (siRNAs) can target the RNA interference (RNAi)
 

Summary:  the activities of RhoA and Cdc42 were unaffected by MMP3, the authors found that a previously identified splice... shown that active Rac can stimulate the production and release of mitochondrial superoxide... or uracil from G·T and G·U mismatches and remains stably bound to the resulting abasic site until

  

Source: Nelson, Celeste M. - Departments of Molecular Biology & Chemical Engineering, Princeton University

 

Collection: Biology and Medicine ; Biotechnology

 
67 news and views Type II restriction endonucleases have
 

Summary:  group oxygen. The active sites of the restriction endonuclease structures that have been previously... . Fig. 1 Observed active site alignment and interactions for a complex of three endonucleases... news and views Type II restriction endonucleases have served as models for understanding ...

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
68 Coevolution of a Homing Endonuclease and Its Host Target Sequence
 

Summary:  of a DNA target is followed by subsequent vertical transmission, even- tual loss of endonuclease activity... , and the subsequent expression and nuclear localization of high levels of active endonuclease do not appear to cause... transmission and expansion across closely related homologous genes, eventual ...

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
69 SURVEY AND SUMMARY Comparative analysis of editosome proteins in
 

Summary:  . Endonuclease activity has not been demonstrated for any of these proteins and thus it is uncertain if some... of a dsRBM, indenti®es these three proteins as those most likely to possess endonuclease activity... , suggests either a modi®cation or loss of endonuclease ...

  

Source: Schnaufer, Achim - Institute of Immunology and Infection Research, University of Edinburgh

 

Collection: Biology and Medicine

 
70 Folding, DNA Recognition, and Function of GIY-YIG Endonucleases
 

Summary:  of the active site resembles the HNH endonuclease family. The struc- ture illustrates how the GIY-YIG scaffold... -YIG endonuclease domain appears to be involved in DNA proofreading coupled to the activity of DNA polymerase III... in UvrC and T4 endonuclease II). A sixth ...

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
71 Nucleic Acids Research, 2007, 113 doi:10.1093/nar/gkm867
 

Summary:  that a homodimeric homing endonuclease would display binding and cleavage activity towards palindromic variants... ) Structural parsimony in endonuclease active sites: should the number of homing endonuclease families... recognition by homing endonucleases Jennifer H. ...

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
72 Pure Appl. Chem., Vol. 83, No. 3, pp. 677686, 2011. doi:10.1351/PAC-CON-10-09-28
 

Summary:  on noncovalent interactions between a spin-labeled nucleobase and an abasic site in duplex DNA. Keywords: EPR... catalytically active molecule before the emergence of proteins. Information about the structure and motion... Postsynthetic spin labeling of nucleobases by click chemistry: Detection of abasic sites The success

  

Source: Sigurdsson, Snorri Thor - Chemistry Division, Science Institute, University of Iceland.

 

Collection: Chemistry

 
73 APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Aug. 2003, p. 48234829 Vol. 69, No. 8 0099-2240/03/$08.00 0 DOI: 10.1128/AEM.69.8.48234829.2003
 

Summary:  Rights Reserved. Fidelity of Select Restriction Endonucleases in Determining Microbial Diversity... May 2003 An evaluation of 18 DNA restriction endonucleases for use in terminal-restriction fragment... to classify restriction endonucleases based upon the ability to resolve unique terminal-restriction fragments

  

Source: Moyer, Craig - Department of Biology, Western Washington University

 

Collection: Environmental Sciences and Ecology ; Biology and Medicine

 
74 Name: pDR-GFPuniv Description: Plasmid for in vivo recombination assays. Based on the original pDR-GFP plasmid described
 

Summary:  ) endonuclease target sites in order to assess in vivo activity of an endonuclease. The plasmid contains two non... to receive (homing) endonuclease target sites. The recognition site for the endonuclease of interest must... recombination event with the 3' truncated copy of the eGFP which ...

  

Source: Monnat, Ray - Department of Genome Sciences, University of Washington at Seattle

 

Collection: Biology and Medicine

 
75 Isolation and Characterization of New Homing Endonuclease Specificities at Individual Target Site
 

Summary:  endonuclease activity and specificity. q 2004 Elsevier Ltd. All rights reserved. Keywords: engineering... -CreI, we next screened for I-CreI derivatives with increased activities against these sites. Endonuclease... against these sites. A single endonuclease mutant with increased ...

  

Source: Monnat, Ray - Department of Genome Sciences, University of Washington at Seattle; Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
76 A Core Folding Model for Catalysis by the Hammerhead Ribozyme Accounts for Its Extraordinary Sensitivity to Abasic Mutations
 

Summary:  decrease in activity (see below). The characterization of the abasic hammerhead variants reported herein... hammerhead isolates (2). (B) Reduced activity of abasic HH16 variants. The effects of abasic modifications... experiments. For the wild-type HH16, as well as for the ...

  

Source: Herschlag, Dan - Department of Biochemistry, Stanford University

 

Collection: Biology and Medicine

 
77 Potential Multiple Endonuclease Functions and a Ribonuclease H Encoded in Retroposon Genomes
 

Summary:  January 22, 2002 Among the retroposons, the source of the endonuclease activity is known to be variable... and an endonuclease activity, some also encode the RH domain. Margaret Dayhoff first introduced the idea that amino... types of endonuclease activ- ities have ...

  

Source: McClure, Marcie - Center for Computational Biology & Department of Microbiology, Montana State University

 

Collection: Biology and Medicine

 
78 Annu. Rev. Microbiol. 2002. 56:26387 doi: 10.1146/annurev.micro.56.012302.160741
 

Summary:  are I for intron and F for freestanding (2). For example, PI MgaI denotes the endonuclease activity... C and E are the dodecapeptide motifs required for endonuclease activity (32, 42). TABLE 2 Comparison... , there will be no more selection for function- ing endonuclease ...

  

Source: Gogarten, J. Peter - Department of Ecology and Evolutionary Biology, University of Connecticut

 

Collection: Environmental Sciences and Ecology

 
79 int. j. radiat. biol 2001, vol. 77, no. 2, 155 164 DNA strand break yields after post-high LET irradiation
 

Summary:  irradiation incubation with endonuclease-III and evidence for hydroxyl radical clustering J. R. MILLIGAN*, J... is that tion of plasmid DNA with the endonuclease-III (endo-III) of multiple chemical modi cations of DNA... , Newman et al. 1997).inhibition of the activity of endo-III by the nearby DNA damage Considerable eÚ ort

  

Source: Nevis Laboratories, Radiological Research Accelerator Facility

 

Collection: Fission and Nuclear Technologies ; Biology and Medicine

 
80 69886998 Nucleic Acids Research, 2008, Vol. 36, No. 22 Published online 4 November 2008 doi:10.1093/nar/gkn846
 

Summary:  with gradual accumula- tion of mutations that lead to loss of endonuclease activity in individual hosts... in bio- chemical studies of endonuclease activity and specificity (described below). To increase... chromatography, also implying that the endonuclease is an ...

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine


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81 Stimulation of RTH1 Nuclease of the Yeast Saccharomyces cereVisiae by Replication Protein A
 

Summary:  in the function of RTH1 nuclease, including its structure specific endonuclease activity. Initial in Vitro studies... oligonucleotides. Yeast RPA stimulated the endonuclease activity of RTH1 endonuclease with these substrates... #12;endonuclease ...

  

Source: McQuade, D. Tyler - Department of Chemistry and Biochemistry, Florida State University

 

Collection: Chemistry

 
82 Homing endonuclease I-CreI derivatives with novel DNA target specificities
 

Summary:  (Figure 2). No endonuclease displayed increased activity towards either the C:G ±4 or the G:C ±4 sites... 26C/Y66R mutant has been described previously (14). Table 3. Active endonuclease derivatives... . The only active endonuclease was the K28R mutant. Like ...

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
83 Generation of Highly Site-Specific DNA Double-Strand Breaks in Human Cells by the Homing Endonucleases
 

Summary:  Endonucleases I-PpoI and I-CreI Raymond J. Monnat, Jr.,*, ,1 Alden F. M. Hackmann,* and Michael A. Cantrell*,2... endonucleases, I-PpoI from the myxomycete Physarum polycephalum and I-CreI from the green alga Chlamydomonas... endonucleases can be expressed in human cells and can generate site- specific DNA double-strand breaks ...

  

Source: Monnat, Ray - Department of Genome Sciences, University of Washington at Seattle

 

Collection: Biology and Medicine

 
84 Long interspersed elements (LINE-1s) are abundant retro-transposons in mammalian genomes that probably retro-
 

Summary:  of the two endonuclease active site mutants (Asp205Ala and His230Ala) and the reverse transcriptase active... in the putative endonuclease active site (Asp205Ala and His230Ala) showed some retrotransposition (approximately... demonstrated that each of these mutations eliminates LINE-1 ...

  

Source: Batzer, Mark A. - Department of Biological Sciences, Louisiana State University

 

Collection: Biotechnology ; Biology and Medicine

 
85 Interstrand crosslink repair: can XPF-ERCC1 be let off the hook?
 

Summary:  that the endonuclease xeroderma pigmentosum complementation group F-excision repair cross-comple- menting rodent repair... to mutagenic lesions, such as pyrimidine dimers and abasic sites, unrepaired DNA ICLs are highly cytotoxic [1... strands that transition from 50 double-stranded to 30 single-stranded DNA. During NER, this ...

  

Source: Sekelsky, Jeff - Department of Biology, University of North Carolina at Chapel Hill

 

Collection: Biology and Medicine

 
86 Catalytic Mechanisms of Restriction and Homing Endonucleases Eric A. Galburt and Barry L. Stoddard*
 

Summary:  future directions in the study of endonuclease active sites. The discussion of type II restriction... endonucleases is comprised of a description of the general architecture of the canonical active site structural... atom to an activated water molecule. Both homing and restriction ...

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
87 Molecular Cell, Vol. 2, 469476, October, 1998, Copyright 1998 by Cell Press DNA Recognition and Cleavage
 

Summary:  of this guanosine. Endonuclease Active Site In the cocrystal structure, two calcium ions (which sub- stitute... abolish Approximately half of the base pairs are strongly con-I-CreI endonuclease activity (Seligman et al... -form DNA was docked to the of PI-SceI, a homing endonuclease with ...

  

Source: Monnat, Ray - Department of Genome Sciences, University of Washington at Seattle; Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
88 New Concepts in Biochemistry Deoxyribose Phosphate Excision by the N-Terminal Domain of the Polymerase
 

Summary:  functions. For example, bacterial endonuclease III (endo III) is a bifunctional enzyme with activities... an activity for excising a 5-deoxyribose phosphate (dRP) group from preincised apurine/apyrimidine (AP) sites... and biochemical data, we present here a reaction mechanism for the 5-dRP excision activity ...

  

Source: Crasto, Chiquito - Department of Genetics, University of Alabama at Birmingham

 

Collection: Biology and Medicine

 
89 798 volume 16 number 8 august 2009 nature structural & molecular biology Shan Zha, Cristian Boboila, Frederick W. Alt
 

Summary:  both single-stranded DNA endonuclease activity7 and 35 exonuclease activity8 (Fig. 2). Mre11 nuclease... activity functions in conjunction with other factors to generate the 3 single-stranded DNA overhang... efficiently activates Atm kinase, which has allowed dissection of this function ...

  

Source: Alt,, Frederick - Immune Disease Institute, Harvard University

 

Collection: Biology and Medicine

 
90 Activity and Specificity of the Bacterial PD-(D/E)XK Homing Endonuclease I-Ssp6803I
 

Summary:  Activity and Specificity of the Bacterial PD-(D/E)XK Homing Endonuclease I-Ssp6803I Lei Zhao1... : Zhao, L., et al., Activity and Specificity of the Bacterial PD-(D/E)XK Homing Endonuclease I-Ssp6803I... ., Activity and Specificity of the Bacterial PD-(D/E)XK Homing ...

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
91 The restriction fold turns to the dark side: a bacterial homing endonuclease with
 

Summary:  ' and `HNH' endonucleases are found in protists and phage, respectively. They share similar active sites... of such a transformation as the basis for a screen for inactivating mutations in the endonuclease active site. We designed... -(D/E)-XK fold with Asp 8 and Glu 11 both involved in the structure and ...

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
92 Engineered Extrahelical Base Destabilization Enhances Sequence Discrimination of DNA Methyltransferase
 

Summary:  by disrupting interactions at a hydrophobic interface between the active site of the enzyme and a highly... destabilize the positioning of the extrahelical, "flipped" cytosine base within the active site. The ternary... disrupts the interface between the loop and the active site, thereby destabilizing the extrahelical target

  

Source: Reich, Norbert O. - Department of Chemistry and Biochemistry, University of California at Santa Barbara

 

Collection: Chemistry ; Biology and Medicine

 
93 MOLECULAR AND CELLULAR BIOLOGY, 0270-7306/00/$04.00 0
 

Summary:  encoded by Tx1L. Further characterization revealed that the endonuclease remains active for many hours... activity is required for active transposition in cultured cells. To date, two types of NLR endonucleases... sites (31); most others probably lack genuine AP ...

  

Source: Christensen, Shawn M. - Department of Biology, University of Texas at Arlington

 

Collection: Biology and Medicine

 
94 In vivo Recombination After Chronic Damage Exposure Falls to Below Spontaneous Levels in ``Recombomice''
 

Summary:  proteins are induced in tissues from chronically irradiated animals (specifically AP endonuclease... exposures occur under chronic conditions. Given that homologous recombinational repair is most active during... of recombinogenic strand breaks and abasic sites. As a first step toward revealing the molecular basis

  

Source: Engelward, Bevin - Division of Bioengineering, Massachusetts Institute of Technology (MIT)

 

Collection: Biology and Medicine

 
95 Copyright 2004 by the Genetics Society of America Homology Modeling and Mutational Analysis of Ho Endonuclease of Yeast
 

Summary:  to catalytic, active-site residues in PI-SceI and other related homing endonucleases are essential for Ho... and the zinc finger domain have evolved a critical role in Ho activity. HO endonuclease initiates a mating... for activity nized by endonuclease domain II; ...

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
96 Molecular Cell Short Article
 

Summary:  describing how the interaction with MutS and the processivity clamp could license the endonuclease activity... discrimination is achieved by mismatch-provoked activation of the MutH endonuclease, which cleaves... motif that is required for endonuclease activity. ...

  

Source: Guarne, Alba - Department of Biochemistry and Biomedical Sciences, McMaster University

 

Collection: Biology and Medicine

 
97 Protein Science (1997), 6:2677-2680. Cambridge University Press. Printed in the USA Copyright 0 1997 The Protein Society
 

Summary:  folding and the structure of the endonuclease active site (Duan et al., 1997; Heath et al., 1997). Several... unsuccessful.We subsequentlyfound that soluble,activeI-PpoI endonuclease could be isolated by refolding... M at the time of IPTG induction to promote the synthesis of soluble, ...

  

Source: Monnat, Ray - Department of Genome Sciences, University of Washington at Seattle; Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine

 
98 Proc. Natl. Acad. Sci. USA Vol. 94, pp. 1308713092, November 1997
 

Summary:  is followed by strand cleavage in the vicinity of the abasic site (by AP endonuclease or AP lyase... at abasic sites and analyzed by denaturing PAGE. DNA glycosylase activity is indicated by the appearance... , that is active against 3MeA, Hx, and A. MATERIALS AND METHODS Reagents. ...

  

Source: Engelward, Bevin - Division of Bioengineering, Massachusetts Institute of Technology (MIT)

 

Collection: Biology and Medicine

 
99 Comparison of methods for melt detection over Greenland using active and passive microwave measurements
 

Summary:  Comparison of methods for melt detection over Greenland using active and passive microwave... layer of wet snow to a single channel melt detection threshold. The model can be applied to both active... . These winter mean images are the estimates of s0 dry and Tdry b used in the implementation of a-based melt

  

Source: Long, David G. - Department of Electrical and Computer Engineering, Brigham Young University

 

Collection: Engineering

 
100 Supplementary information for ' Coevolution of homing endonuclease specificity and its host target sequence'
 

Summary:  Supplementary information for ' Coevolution of homing endonuclease specificity and its host target... in the main text, and as shown in supplementary Figure S2. Metal binding in the I-AniI active site... . The precise use of bound divalent metal ions for cleavage by LAGLIDADG endonucleases enzymes is still somewhat

  

Source: Stoddard, Barry - Division of Basic Sciences, Fred Hutchinson Cancer Research Center, University of Washington at Seattle

 

Collection: Biology and Medicine


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