Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser
Journal Article
·
· Physical Review. B, Condensed Matter and Materials Physics
- Max-Planck Institut fuer medizinische Forschung, Jahnstrasse 29, DE-69120 Heidelberg (Germany)
- Center for Free-Electron Laser Science, DESY, Notkestrasse 85, DE-22607 Hamburg (Germany)
X-ray free-electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction of the sample by radiation damage. Diffraction intensities of lysozyme nanocrystals collected at the Linac Coherent Light Source using 2 keV photons were used for structure determination by molecular replacement and analyzed for radiation damage as a function of pulse length and fluence. Signatures of radiation damage are observed for pulses as short as 70 fs. Parametric scaling used in conventional crystallography does not account for the observed effects.
- OSTI ID:
- 21596929
- Journal Information:
- Physical Review. B, Condensed Matter and Materials Physics, Vol. 84, Issue 21; Other Information: DOI: 10.1103/PhysRevB.84.214111; (c) 2011 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA); ISSN 1098-0121
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
BIOLOGICAL RADIATION EFFECTS
CRYSTALLOGRAPHY
DIFFRACTION
FREE ELECTRON LASERS
KEV RANGE 01-10
LINEAR ACCELERATORS
LYSOZYME
NANOSTRUCTURES
PROTEINS
PULSES
RESOLUTION
X RADIATION
ACCELERATORS
BIOLOGICAL EFFECTS
COHERENT SCATTERING
ELECTROMAGNETIC RADIATION
ENERGY RANGE
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
IONIZING RADIATIONS
KEV RANGE
LASERS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
SCATTERING
SUPERCONDUCTIVITY AND SUPERFLUIDITY
BIOLOGICAL RADIATION EFFECTS
CRYSTALLOGRAPHY
DIFFRACTION
FREE ELECTRON LASERS
KEV RANGE 01-10
LINEAR ACCELERATORS
LYSOZYME
NANOSTRUCTURES
PROTEINS
PULSES
RESOLUTION
X RADIATION
ACCELERATORS
BIOLOGICAL EFFECTS
COHERENT SCATTERING
ELECTROMAGNETIC RADIATION
ENERGY RANGE
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
IONIZING RADIATIONS
KEV RANGE
LASERS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
SCATTERING