Bibliographic Citation
| Document |  2 Mb |
|---|---|
| Title | TiO2 Nanoparticles as a Soft X-ray Molecular Probe |
| Creator/Author | Larabell, Carolyn ; Ashcroft, Jared M. ; Gu, Weiwei ; Zhang, Tierui ; Hughes, Steven M. ; Hartman, Keith B. ; Hofmann, Cristina ; Kanaras, Antonios G. ; Kilcoyne, David A. ; Le Gros, Mark ; Yin, Yadong ; Alivisatos, A. Paul ; Larabell, Carolyn A. |
| Publication Date | 2007 Jun 30 |
| OSTI Identifier | OSTI ID: 927892 |
| Report Number(s) | LBNL-217E |
| DOE Contract Number | DE-AC02-05CH11231 |
| Other Number(s) | TRN: US200816%%1119 |
| Resource Type | Journal Article |
| Resource Relation | Journal Name: Chemical Communications |
| Research Org | Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US) |
| Sponsoring Org | Materials Sciences Division; Physical Biosciences Division |
| Subject | 37; 36; ABSORPTION; COLLOIDS; MICROSCOPY; MICROTUBULES; PRESSING; PROBES; PROTEINS; SPECTROSCOPY |
| Related Subject | TiO2, soft x-ray microscopy, nanocrystals, molecular probes |
| Description/Abstract | With the emergence of soft x-ray techniques for imaging cells, there is a pressing need to develop protein localization probes that can be unambiguously identified within the region of x-ray spectrum used for imaging. TiO2 nanocrystal colloids, which have a strong absorption cross-section within the "water-window" region of x-rays, areideally suited as soft x-ray microscopy probes. To demonstrate their efficacy, TiO2-streptavidin nanoconjugates were prepared and subsequently labeled microtubules polymerized from biotinylated tubulin. The microtubules were imaged using scanning transmission x-ray microscopy (STXM), and the TiO2 nanoparticle tags were specifically identified using x-ray absorption near edge spectroscopy (XANES). These experiments demonstrate that TiO2 nanoparticles are potential probes for protein localization analyses using soft x-ray microscopy. |
| Country of Publication | United States |
| Language | English |
| Format | Medium: ED |
| System Entry Date | 2008 Sep 02 |
Top | |
Last Updated: 11/19/2009