|
With the emergence of soft x-ray techniques for imaging cells, there is a pressing need to develop protein localization probes that can be unambiguously identified within the region of x-ray spectrum used for imaging. TiO2 nanocrystal colloids, which have a strong absorption cross-section within the "water-window" region of x-rays, areideally suited as soft x-ray microscopy probes. To demonstrate their efficacy, TiO2-streptavidin nanoconjugates were prepared and subsequently labeled microtubules polymerized from biotinylated tubulin. The microtubules were imaged using scanning transmission x-ray microscopy (STXM), and the TiO2 nanoparticle tags were specifically identified using x-ray absorption near edge spectroscopy (XANES). These experiments demonstrate that TiO2 nanoparticles are potential probes for protein localization analyses using soft x-ray microscopy.
|
| Authors: |
Larabell, Carolyn;
Ashcroft, Jared M.;
Gu, Weiwei;
Zhang, Tierui;
Hughes, Steven M.;
Hartman, Keith B.;
Hofmann, Cristina;
Kanaras, Antonios G.;
Kilcoyne, David A.;
Le Gros, Mark;
Yin, Yadong;
Alivisatos, A. Paul;
Larabell, Carolyn A.
|
| Publication Date: | 2007 Jun 30 |
|---|
| OSTI Identifier: | 927892 |
|---|
| Report Number(s): | LBNL-217E |
|---|
| DOE Contract Number: | DE-AC02-05CH11231 |
|---|
| Resource Type: | Journal Article |
|---|
| Resource Relation: | Journal Name: Chemical Communications |
|---|
| Research Org: | Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US) |
|---|
| Sponsoring Org: | Materials Sciences Division; Physical Biosciences Division |
|---|
| Country of Publication: | United States |
|---|
| Language: | English |
|---|
| Other Number(s): | TRN: US200816%%1119 |
|---|
| Subject: | 37; 36; ABSORPTION; COLLOIDS; MICROSCOPY; MICROTUBULES; PRESSING; PROBES; PROTEINS; SPECTROSCOPY |
|---|
| Related Subject: | TiO2, soft x-ray microscopy, nanocrystals, molecular probes |
|---|
| Update Date: | 2009 Dec 16 |
|---|
Top |
|
2 Mb