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Title TiO2 Nanoparticles as a Soft X-ray Molecular Probe
Creator/Author Larabell, Carolyn ; Ashcroft, Jared M. ; Gu, Weiwei ; Zhang, Tierui ; Hughes, Steven M. ; Hartman, Keith B. ; Hofmann, Cristina ; Kanaras, Antonios G. ; Kilcoyne, David A. ; Le Gros, Mark ; Yin, Yadong ; Alivisatos, A. Paul ; Larabell, Carolyn A.
Publication Date2007 Jun 30
OSTI IdentifierOSTI ID: 927892
Report Number(s)LBNL-217E
DOE Contract NumberDE-AC02-05CH11231
Other Number(s)TRN: US200816%%1119
Resource TypeJournal Article
Resource RelationJournal Name: Chemical Communications
Research OrgErnest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US)
Sponsoring OrgMaterials Sciences Division; Physical Biosciences Division
Subject37; 36; ABSORPTION; COLLOIDS; MICROSCOPY; MICROTUBULES; PRESSING; PROBES; PROTEINS; SPECTROSCOPY
Related SubjectTiO2, soft x-ray microscopy, nanocrystals, molecular probes
Description/AbstractWith the emergence of soft x-ray techniques for imaging cells, there is a pressing need to develop protein localization probes that can be unambiguously identified within the region of x-ray spectrum used for imaging. TiO2 nanocrystal colloids, which have a strong absorption cross-section within the "water-window" region of x-rays, areideally suited as soft x-ray microscopy probes. To demonstrate their efficacy, TiO2-streptavidin nanoconjugates were prepared and subsequently labeled microtubules polymerized from biotinylated tubulin. The microtubules were imaged using scanning transmission x-ray microscopy (STXM), and the TiO2 nanoparticle tags were specifically identified using x-ray absorption near edge spectroscopy (XANES). These experiments demonstrate that TiO2 nanoparticles are potential probes for protein localization analyses using soft x-ray microscopy.
Country of PublicationUnited States
LanguageEnglish
FormatMedium: ED
System Entry Date2008 Sep 02

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