Bibliographic Citation
| Document | For copies of Journal Articles, please contact the Publisher or your local public or university library and refer to the information in the Resource Relation field. For copies of other documents, please see the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or Document Availability. |
|---|---|
| DOI | 10.2307/3579028 |
| Title | Heterogeneity in c-jun gene expression in normal and malignant cells exposed to either ionizing radiation or hydrogen peroxide |
| Creator/Author | Collart, F.R. ; Horio, M. ; Huberman, E. [Argonne National Laboratory, IL (United States)] |
| Publication Date | 1995 May 01 |
| OSTI Identifier | OSTI ID: 81182 |
| DOE Contract Number | W-31109-ENG-38 |
| Other Number(s) | Journal ID: RAREAE; ISSN 0033-7587; TRN: TRN: 95:004462-0009 |
| Resource Type | Journal Article |
| Resource Relation | Journal Name: Radiation Research; Journal Volume: 142; Journal Issue: 2; Other Information: PBD: May 1995 |
| Research Org | Argonne National Laboratory (ANL), Argonne, IL |
| Subject | 56 BIOLOGY AND MEDICINE, APPLIED STUDIES; FIBROBLASTS; BIOLOGICAL RADIATION EFFECTS; OXYGEN; RESPONSE MODIFYING FACTORS; TUMOR CELLS; GENE REGULATION; INDUCTION; COMPARATIVE EVALUATIONS; PHOSPHOTRANSFERASES; HYDROGEN PEROXIDE; LEUKEMIA; IONIZING RADIATIONS |
| Description/Abstract | We investigated the role of reactive oxygen intermediates and protein kinase C in the induction of expression of the c-jun gene in human ML-2 leukemic cells and normal human DET-551 fibroblasts by comparing the effects of exposure to either ionizing radiation or H{sub 2}O{sub 2} in the presence or absence of appropriate inhibitors. In these cell types, the radiation-and H{sub 2}O{sub 2}-mediated increase in c-jun mRNA levels could be prevented by pretreatment of the cells with N-acetylcysteine, and antioxidant, or H7, an inhibitor of protein kinase C and protein kinase A, but not by HA1004, a specific inhibitor of protein kinase A and G. These results suggest a role for protein kinase C and reactive oxygen intermediates in the induction of c-jun gene expression in both normal and tumor cells. We also investigated potential differences in c-jun gene expression induced by radiation or H{sub 2}O{sub 2} in normal and tumor cells by examining steady-state c-jun mRNA levels in a number of human fibroblast, leukemia, melanoma, sarcoma and carcinoma cell types. We observed heterogeneity in the steady-state level of c-jun mRNA in both the untreated normal and tumor cells and in such cells exposed to ionizing radiation or to H{sub 2}O{sub 2}. Exposure to radiation produced a varied response which ranged from little or no induction to an increase in the steady-state level of the c-jun mRNA of more than two orders of magnitude. Exposure to H{sub 2}O{sub 2} gave a pattern similar to that of ionizing radiation. The basis for the differential induction in response to these agents may be attributable to either cell lineage or genetic heterogeneity or a combination of these two parameters. 30 refs., 7 figs., 1 tab. |
| Country of Publication | United States |
| Language | English |
| Format | Medium: X; Size: pp. 188-195 |
| System Entry Date | 2009 Mar 09 |
Top | |
