Bibliographic Citation
| Document | For copies of Journal Articles, please contact the Publisher or your local public or university library and refer to the information in the Resource Relation field. For copies of other documents, please see the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or Document Availability. |
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| DOI | http://dx.doi.org/10.1101/gr.GR-1780R |
| Title | Single nucleotide polymorphism markers for genetic mapping in Drosophila melanogaster |
| Creator/Author | Hoskins, Roger A. ; Phan, Alexander C. ; Naeemuddin, Mohammed ; Mapa, Felipa A. ; Ruddy, David A. ; Ryan, Jessica J. ; Young, Lynn M. ; Wells, Trent ; Kopczynski, Casey ; Ellis, Michael C. |
| Publication Date | 2001 Apr 16 |
| OSTI Identifier | OSTI ID: 785285 |
| Report Number(s) | LBNL--47759 |
| DOE Contract Number | AC03-76SF00098 |
| Other Number(s) | R&D Project: 803NBE; TRN: AH200131%%160 |
| Resource Type | Journal Article |
| Resource Relation | Journal Name: Genome Research; Journal Volume: 11; Journal Issue: 6; Other Information: Journal Publication Date: June 2001; PBD: 16 Apr 2001 |
| Research Org | Lawrence Berkeley National Lab., CA (US) |
| Sponsoring Org | National Institutes of Health (US) |
| Subject | 59 BASIC BIOLOGICAL SCIENCES; DROSOPHILA; EFFICIENCY; GENES; GENETIC MAPPING; GENETICS; MUTATIONS; NUCLEOTIDES; RECOMBINATION; RESOLUTION; STRAINS; X CHROMOSOME |
| Related Subject | DROSOPHILA SNP GENETIC MAPPING |
| Description/Abstract | For nearly a century, genetic analysis in Drosophila melanogaster has been a powerful tool for analyzing gene function, yet Drosophila lacks the molecular genetic mapping tools that have recently revolutionized human, mouse and plant genetics. Here, we describe the systematic characterization of a dense set of molecular markers in Drosophila using an STS-based physical map of the genome. We identify 474 biallelic markers in standard laboratory strains of Drosophila that the genome. The majority of these markers are single nucleotide polymorphisms (SNPs) and sequences for these variants are provided in an accessible format. The average density of the new markers is 1 marker per 225 kb on the autosomes and 1 marker per 1 Mb on the X chromosome. We include in this survey a set of P-element strains that provide additional utility for high-resolution mapping. We demonstrate one application of the new markers in a simple set of crosses to map a mutation in the hedgehog gene to an interval of <1 Mb. This new map resource significantly increases the efficiency and resolution of recombination mapping and will be of immediate value to the Drosophila research community. |
| Country of Publication | United States |
| Language | English |
| Format | Medium: X; Size: vp. |
| System Entry Date | 2008 Feb 05 |
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Last Updated: 11/19/2009