Bibliographic Citation
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| Title | Retinoic acid and 1,25-dihydroxyvitamin D3 stimulate osteoclast formation by different mechanisms |
| Creator/Author | Scheven, B.A. ; Hamilton, N.J. (Rowett Research Institute, Bucksburn, Aberdeen (Scotland)) |
| Publication Date | 1990 Jan 01 |
| OSTI Identifier | OSTI ID: 6871389 |
| Other Number(s) | Journal ID: ISSN 8756-3282; CODEN: BONED |
| Resource Type | Journal Article |
| Resource Relation | Journal Name: Bone; (USA); Journal Volume: 11:1 |
| Subject | 59 BASIC BIOLOGICAL SCIENCES; BONE CELLS; CELL PROLIFERATION; RETINOIC ACID; BIOCHEMICAL REACTION KINETICS; VITAMIN D; AUTORADIOGRAPHY; BONE MARROW; CALCIUM; CELL NUCLEI; FETUSES; PRECURSOR; RATS; THYMIDINE; TISSUE CULTURES; TRITIUM COMPOUNDS; ALKALINE EARTH METALS; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; AZINES; BODY; CARBOXYLIC ACID ESTERS; CELL CONSTITUENTS; CONNECTIVE TISSUE CELLS; ELEMENTS; ESTERS; HEMATOPOIETIC SYSTEM; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; KINETICS; MAMMALS; METALS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PYRIMIDINES; REACTION KINETICS; RIBOSIDES; RODENTS; SOMATIC CELLS; TISSUES; VERTEBRATES; VITAMINS |
| Description/Abstract | The effects of retinoic acid (RA) and 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on osteoclast formation were examined in intact fetal long bones of different ages/developmental stages maintained in organ culture using a chemically defined medium with or without the presence of serum. Besides stimulating bone resorption, RA and 1,25-(OH)2D3 increased the number of osteoclasts in 19-day-old fetal rat tibiae. Likewise, these bone-resorbing agents induced and stimulated osteoclast formation in 19- and 18-day-old metatarsal bones which were osteoclast-free at the beginning of the culture. The response to 1,25-(OH)2D3 was greatly enhanced by 10% fetal bovine serum (FBS) irrespective of the developmental stage of the long bone. The response to RA was not. Light microscopic autoradiography after labeling of the cultures with tritiated thymidine showed that both RA and 1,25-(OH)2D3 induced osteoclast differentiation from proliferating and postmitotic precursors. However, neither agent was able to stimulate proliferation of osteoclast progenitor cells in the older bones (19 days). Studies on the formation of osteoclast-like (tartrate-resistant acid phosphatase positive) cells in bone marrow cultures indicated that FBS was a potent inducer of osteoclast-like cell formation. In the presence of FBS, 1,25-(OH)2D3 significantly stimulated this response, but RA did not. The results demonstrate that although both RA and 1,25-(OH)2D3 stimulate osteoclast formation from proliferating and postmitotic precursors in long bones in vitro, they do so by different mechanisms. |
| Country of Publication | United States |
| Language | English |
| Format | Medium: X; Size: Pages: 53-59 |
| System Entry Date | 2009 Dec 17 |
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