Bibliographic Citation
| Document | For copies of Journal Articles, please contact the Publisher or your local public or university library and refer to the information in the Resource Relation field. For copies of other documents, please see the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or Document Availability. |
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| Title | An enzyme-linked immunoabsorbent assay for estimating red cell survival of transfused red cells-validation using CR-51 labeling |
| Creator/Author | Drew, H. ; Kickler, T. ; Smith, B. ; LaFrance, N. |
| Publication Date | 1984 Jan 01 |
| OSTI Identifier | OSTI ID: 6781878 |
| Report Number(s) | CONF-840619- |
| Other Number(s) | Journal ID: CODEN: JNMEA |
| Resource Type | Conference |
| Specific Type | Journal Article |
| Resource Relation | Journal Name: J. Nucl. Med.; (United States); Journal Volume: 25:5; Conference: 31. annual meeting of the Society of Nuclear Medicine, Los Angeles, CA, USA, 5 Jun 1984 |
| Research Org | The Johns Hopkins Medical Institutions, Baltimore, MD |
| Subject | 62 RADIOLOGY AND NUCLEAR MEDICINE; BLOOD CELLS; BIOLOGICAL HALF-LIFE; SURVIVAL TIME; ANEMIAS; BIOASSAY; CHROMIUM 51; ENZYMES; LABELLING; PATIENTS; TRACER TECHNIQUES; TRANSFUSIONS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BODY FLUIDS; CHROMIUM ISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; HEMIC DISEASES; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; MATERIALS; NUCLEI; RADIOISOTOPES; SYMPTOMS; THERAPY |
| Description/Abstract | The survival time of transfused red cells antigenically distinct from the recipient's red cells was determined using an indirect enzyme linked antiglobulin test. These results were then compared to those determined by Cr-51 labeling. Three patients with hypoproliferative anemias and one patient (2 studies) with traumatic hemolytic anemia caused by a prosthetic heart valve were studied. Survival times were performed by transfusing a 5cc aliquot of Cr-51 labeled cells along with the remaining unit. One hour post transfusion, a blood sample was drawn and used as the 100% value. Subsequent samples drawn over a 2-3 week period were then compared to the initial sample to determine percent survival for both methods. The ELISA method for measuring red cell survival in antigenically distinct cells is in close agreement with the Cr-51 method. Although CR-51 labeling is the accepted method for red cell survival determination the ELISA method can be used when radioisotopes are unavailable or contraindicated or when the decision to estimate red cell survival is made after transfusion. |
| Country of Publication | United States |
| Language | English |
| Format | Medium: X; Size: Pages: 98 |
| System Entry Date | 2008 Feb 08 |
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Last Updated: 11/19/2009