Bibliographic Citation
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| Title | Effects of colchicine on the intestinal transport of endogenous lipid. Ultrastructural, biochemical, and radiochemical studies in fasting rats |
| Creator/Author | Pavelka, M. ; Gangl, A. |
| Publication Date | 1983 Mar 01 |
| OSTI Identifier | OSTI ID: 6225331 |
| Other Number(s) | Journal ID: CODEN: GASTA |
| Resource Type | Journal Article |
| Resource Relation | Journal Name: Gastroenterology; (United States); Journal Volume: 84:3 |
| Research Org | Institute of Micromorphology, University of Vienna, Austria |
| Subject | 59 BASIC BIOLOGICAL SCIENCES; COLCHICINE; BIOLOGICAL EFFECTS; METABOLISM; LIPIDS; INTESTINAL ABSORPTION; BIOCHEMICAL REACTION KINETICS; CARBON 14 COMPOUNDS; CARBOXYLIC ACIDS; ELECTRON MICROSCOPY; LINOLENIC ACID; RATS; SMALL INTESTINE; TRACER TECHNIQUES; ABSORPTION; ALKALOIDS; ANIMALS; ANTIMITOTIC DRUGS; ANTIPYRETICS; BODY; CENTRAL NERVOUS SYSTEM DEPRESSANTS; DIGESTIVE SYSTEM; DRUGS; GASTROINTESTINAL TRACT; INTESTINES; ISOTOPE APPLICATIONS; KINETICS; LABELLED COMPOUNDS; MAMMALS; MICROSCOPY; MONOCARBOXYLIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; REACTION KINETICS; RODENTS; UPTAKE; VERTEBRATES |
| Description/Abstract | The involvement of microtubules in the transepithelial transport of exogenous lipid in intestinal absorptive cells has been suggested. Using electronmicroscopic, biochemical, and radiochemical methods, researchers have studied the effects of the antimicrotubular agent colchicine on the intestinal mucosa and on the intestinal transport of endogenous lipid of rats in the fasting state. After colchicine treatment, the concentration of triglycerides in intestinal mucosa of rats fasted for 24 h doubled, and electron microscopic studies showed a striking accumulation of lipid particles in absorptive epithelial cells of the tips of jejunal villi. These findings suggest that colchicine interferes with the intestinal transepithelial transport of endogenous lipoproteins. Additional studies, using an intraduodenal pulse injection of (/sup 14/C)linoleic acid, showed that colchicine does not affect the uptake of fatty acids by intestinal mucosa. However, it had divergent effects on fatty acid esterification, enhancing their incorporation into triglycerides relative to phospholipids, and caused a significant accumulation of endogenous diglycerides, triglycerides, and cholesterol esters within the absorptive intestinal epithelium. Detailed ultrastructural and morphometric studies revealed a decrease of visible microtubules, and a displacement of the smooth and rough endoplasmic reticulum and Golgi apparatus. Furthermore, it is shown that after colchicine treatment, microvilli appear at the lateral plasma membrane of intestinal absorptive cells, a change not previously reported to our knowledge. Thus, our study shows that colchicine causes significant changes in enterocyte ultrastructure and colchicine perturbs the reesterification of absorbed endogenous fatty acids and their secretion in the form of triglyceride-rich lipoproteins from the enterocyte. |
| Country of Publication | United States |
| Language | English |
| Format | Medium: X; Size: Pages: 544-555 |
| System Entry Date | 2009 Dec 17 |
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