Cytoplasmic dynein is a minus end-directed motor for membranous organelles
The role of cytoplasmic dynein in microtubule-based organelle transport was examined using a reconstituted assay developed from chick embryo fibroblasts. Factors present in a high-speed cytosol caused the movement of purified organelles on microtubules predominantly in the minus end direction. Inactivation of cytoplasmic dynein in the high-speed cytosol by vanadate-mediated UV photocleavage inhibited minus end-directed organelle motility by over 90%. Addition of purified cytoplasmic dynein to the inactive cytosol restored minus end-directed organelle motility, although purified cytoplasmic dynein by itself did not support organelle movement. We propose that cytoplasmic dynein is the motor for minus end-directed organelle movement, but that additional cytosolic factors are also required to produce organelle motility.
- Research Organization:
- Washington Univ. School of Medicine, St. Louis, MO (USA)
- OSTI ID:
- 6149021
- Journal Information:
- Cell; (United States), Vol. 56:6
- Country of Publication:
- United States
- Language:
- English
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CYTOPLASM
EMBRYOS
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MICROTUBULES
MOLECULAR WEIGHT
ULTRAVIOLET RADIATION
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560120* - Radiation Effects on Biochemicals
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