Bibliographic Citation
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| Title | Hormonal regulation of hepatic glycogenolysis in the carp, Cyprinus carpio |
| Creator/Author | Janssens, P.A. ; Lowrey, P. |
| Publication Date | 1987 Apr 01 |
| OSTI Identifier | OSTI ID: 5879269 |
| Other Number(s) | Journal ID: CODEN: AJPHA |
| Resource Type | Journal Article |
| Resource Relation | Journal Name: Am. J. Physiol.; (United States); Journal Volume: 252:4 |
| Research Org | Australian National Univ., Canberra |
| Subject | 59 BASIC BIOLOGICAL SCIENCES; ADRENAL HORMONES; BIOCHEMISTRY; GLYCOGEN; ENZYMATIC HYDROLYSIS; LABELLED COMPOUNDS; UPTAKE; ADRENALINE; ANGIOTENSIN; BIOCHEMICAL REACTION KINETICS; CATECHOLAMINES; ENZYME ACTIVITY; FISHES; IODINE 125; LIGANDS; LIVER; NORADRENALINE; PEPTIDES; PHOSPHOTRANSFERASES; RECEPTORS; SYMPATHOMIMETICS; VASOPRESSIN; AMINES; ANIMALS; AQUATIC ORGANISMS; AROMATICS; AUTONOMIC NERVOUS SYSTEM AGENTS; BETA DECAY RADIOISOTOPES; BODY; CARBOHYDRATES; CARDIOTONICS; CARDIOVASCULAR AGENTS; CHEMICAL REACTIONS; CHEMISTRY; DAYS LIVING RADIOISOTOPES; DECOMPOSITION; DIGESTIVE SYSTEM; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; ENZYMES; GLANDS; GLOBULINS; HORMONES; HYDROLYSIS; HYDROXY COMPOUNDS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPES; KINETICS; LYSIS; MEMBRANE PROTEINS; NEUROREGULATORS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HORMONES; PHENOLS; PHOSPHORUS-GROUP TRANSFERASES; PITUITARY HORMONES; POLYPHENOLS; POLYSACCHARIDES; PROTEINS; RADIOISOTOPES; REACTION KINETICS; SACCHARIDES; SOLVOLYSIS; TRANSFERASES; VASOCONSTRICTORS; VERTEBRATES |
| Description/Abstract | Carp (Cyprinus carpio) liver maintained normal glycogen content and enzyme complement for several days in organ culture. Epinephrine-stimulated glycogenolysis, phosphorylase activation, and cyclic AMP (cAMP) accumulation in a concentration-dependent manner with EC/sub 50/s of 100, 100, and 500 nM, respectively. These actions were blocked by the ..beta..-adrenergic antagonist, propranolol, but not by the ..cap alpha..-adrenergic antagonist phentolamine. Glycogenolysis and tissue cAMP were uninfluenced by 10/sup -6/ M arginine vasotocin, arginine vasopressin, lysine vasotocin, lysine vasopressin, mesotocin, or oxytocin, but were slightly increased by 10/sup -5/ M isotocin and slightly decreased by 10/sup -6/ M angiotensin II. (/sup 125/I)-iodocyanopindolol (ICP), a ..beta..-adrenergic ligand, bound to isolated carp liver membranes with a K/sub D/ of 83 pM. Maximum binding of 45 fmol/mg protein was at 600 pM. Propranolol, isoprenaline, epinephrine, phenylephrine, norepinephrine, and phenoxybenzamine displaced ICP with K/sub D/s of 100 nM, 2, 20, 20, 60, and 200 ..mu..M, respectively. The ..cap alpha..-adrenergic antagonists, yohimbine and prazosin, showed no specific binding. These data provide evidence that catecholamines act via ..beta..-adrenergic receptors in carp liver and that ..cap alpha..-adrenergic receptors are not present. Vasoactive peptides play no significant role in regulation of carp liver glycogenolysis. |
| Country of Publication | United States |
| Language | English |
| Format | Medium: X; Size: Pages: R653-R660 |
| System Entry Date | 2009 Dec 16 |
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