Mathematical model for the aerobic growth of saccharomyces cerevisiae with a saturated respiratory capacity
A mathematical model for the aerobic growth of Saccharomyces cerevisiae in both batch and continuous culture is described. It was based on the experimental observation that the respiratory capacity of this organism may become saturated and exhibit a maximum specific oxygen uptake rate after suitable adaptation. This experimental observation led to the possibility that transport into and out of the mitochondrion was of major importance in the overall metabolism of S. cerevisiae and was subject to long-term adaptation. Consistent with this observation a distributed model was proposed which, as its basis, assumed the control of respiration and fermentation to be the result of saturation of respiration without any specific repression or inhibition of the uptake rates of other substrates. No other regulation of fermentation and respiration was assumed. The model provided a suitable structure allowing precise quantification of the changes in rate and stoichiometry of energy production. The model clearly indicated that growth under the wide range of experimental conditions reported could not be predicted using constant values for the maximum specific respiratory rate or constant values of Yatp (g biomass/mol ATP) and PO ratio of (mol ATP/atom oxygen). The causes of the variation in the respiratory rate were not determined and it was concluded that a more detailed analysis (reported subsequently) was required. The variation of Y atp and PO ratio with specific growth rate implied that the efficiency of ATP generation or ATP utilization decreased with increasing specific growth rate. It was concluded that it was not possible to quantify the individual effect of Yatp and PO ratio until independent means for their reliable estimation is available. (Refs. 84).
- Research Organization:
- Dept. Chemical Eng., Univ. of Sydney, New South Wales 2006, Australia; Dept. of Biological Process Eng., School of Chem. Eng. and Ind. Chem., Univ. of New South Wales, Kensington, New South Wales 2033, Australia
- OSTI ID:
- 5429213
- Journal Information:
- Biotechnol. Bioeng.; (United States), Vol. 23:8
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
SACCHAROMYCES CEREVISIAE
BATCH CULTURE
CONTINUOUS CULTURE
ETHANOL
FERMENTATION
GROWTH
MATHEMATICAL MODELS
RESPIRATION
ALCOHOLS
BIOCONVERSION
FUNGI
HYDROXY COMPOUNDS
MICROORGANISMS
ORGANIC COMPOUNDS
PLANTS
SACCHAROMYCES
YEASTS
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