Bibliographic Citation
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| Title | Multiple opiate receptors in the brain of spontaneously hypertensive rats |
| Creator/Author | Das, S. ; Bhargava, H.N. |
| Publication Date | 1986 Mar 01 |
| OSTI Identifier | OSTI ID: 5383631 |
| Report Number(s) | CONF-8604222- |
| Other Number(s) | Journal ID: CODEN: FEPRA |
| Resource Type | Conference |
| Specific Type | Journal Article |
| Resource Relation | Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States); Journal Volume: 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986 |
| Research Org | Univ. of Illinois, Chicago |
| Subject | 59 BASIC BIOLOGICAL SCIENCES; CENTRAL NERVOUS SYSTEM DEPRESSANTS; RECEPTORS; HYPERTENSION; PATHOGENESIS; BIOCHEMICAL REACTION KINETICS; BRAIN; LIGANDS; RATS; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ANIMALS; BODY; CARDIOVASCULAR DISEASES; CENTRAL NERVOUS SYSTEM; DISEASES; DRUGS; ISOTOPE APPLICATIONS; KINETICS; LABELLED COMPOUNDS; MAMMALS; MEMBRANE PROTEINS; NERVOUS SYSTEM; ORGANIC COMPOUNDS; ORGANS; PROTEINS; REACTION KINETICS; RODENTS; SYMPTOMS; VASCULAR DISEASES; VERTEBRATES |
| Description/Abstract | The characteristics of ..mu.., delta and kappa -opiate receptors in the brain of spontaneously hypertensive (SH) and normotensive Wistar-Kyoto (WKY) rats were determined using the receptor binding assays. The ligands used were /sup 3/H-naltrexone (..mu..), /sup 3/H-ethylketocyclazocine (EKC, kappa) and /sup 3/H-Tyr-D-Ser-Gly-Phe-Leu-Thr (DSTLE, delta). Since EKC binds to ..mu.. and delta receptors in addition to kappa, the binding was done in the presence of 100 nM each of DAGO and DADLE to suppress ..mu.. and delta sites, respectively. All three ligands bound to brain membranes of WKY rats at a single high affinity site with the following B/sub max/ (fmol/mg protein) and K/sub d/ (nM) values: /sup 3/H-naltrexone (130.5; 0.43) /sup 3/H-EKC (19.8, 1.7) and /sup 3/H-DSTLE (139, 2.5). The binding of /sup 3/H-naltrexone and /sup 3/H-DSTLE in the brain of WKY and SH did not differ. A consistent increase (22%) in B/sub max/ of /sup 3/H-EKC was found in SHR compared to WKY rats. However, the K/sub d/ values did not differ. The increase in B/sub max/ was due to increases in hypothalamus and cortex. It is concluded that SH rats have higher density of kappa-opiate receptors, particularly in hypothalamus and cortex, compared to WKY rats, and that kappa-opiate receptors may be involved in the pathophysiology of hypertension. |
| Country of Publication | United States |
| Language | English |
| Format | Medium: X; Size: Pages: 188 |
| System Entry Date | 2008 Feb 06 |
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