Energy Citations Database
Bookmark and Share

Bibliographic Citation

 
Document
For copies of Journal Articles, please contact the Publisher or your local public or university library and refer to the information in the Resource Relation field.
For copies of other documents, please see the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or Document Availability.
Title Characterization of a ( sub 3 H)-5-hydroxtyryptamine binding site in rabbit caudate nucleus that differs from the 5-HT sub 1A , 5-HT sub 1B , 5-HT sub 1C and 5-HT sub 1D subtypes
Creator/Author Xiong, Wencheng ; Nelson, D.L. (Univ. of Arizona, Tucson (USA))
Publication Date1989 Jan 01
OSTI IdentifierOSTI ID: 5131967
Other Number(s)Journal ID: ISSN 0024-3205; CODEN: LIFSA
Resource TypeJournal Article
Resource RelationJournal Name: Life Sciences; (USA); Journal Volume: 45:16
Subject59 BASIC BIOLOGICAL SCIENCES; RECEPTORS; CHEMICAL COMPOSITION; SEROTONIN; BIOCHEMICAL REACTION KINETICS; CALCIUM; CELL MEMBRANES; CEREBRAL CORTEX; RABBITS; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ALKALINE EARTH METALS; AMINES; ANIMALS; AROMATICS; AUTONOMIC NERVOUS SYSTEM AGENTS; AZAARENES; AZOLES; BODY; BRAIN; CELL CONSTITUENTS; CENTRAL NERVOUS SYSTEM; CEREBRUM; DRUGS; ELEMENTS; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; HYDROXY COMPOUNDS; INDOLES; ISOTOPE APPLICATIONS; KINETICS; MAMMALS; MEMBRANE PROTEINS; MEMBRANES; METALS; NERVOUS SYSTEM; NEUROREGULATORS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PROTEINS; PYRROLES; RADIOPROTECTIVE SUBSTANCES; REACTION KINETICS; SYMPATHOMIMETICS; TRYPTAMINES; VERTEBRATES
Description/Abstract({sup 3}H)5-HT binding sites were analyzed in membranes prepared from the rabbit caudate nucleus (CN). ({sup 3}H)5-HT labeled both 5-HT{sub 1A} and 5-HT{sub 1C} recognition sites, defined by nanomolar affinity for 8-OH-DPAT and mesulergine respectively; however, these represented only a fraction of total specific ({sup 3}H)5-HT binding. Saturation experiments of ({sup 3}H)5-HT binding in the presence of 100 nM 8-OH-DPAT and 100 nM mesulergine to block 5-HT{sub 1A} and 5-HT{sub 1C} sites revealed that non-5-HT{sub 1A}/non-5-HT{sub 1C} sites represented about 60% of the total 5-HT{sub 1} sites and that they exhibited saturable, high affinity, and homogeneous binding. The pharmacological profile of the non-5-HT{sub 1A}/non-5-HT{sub 1C} sites (designated 5-HT{sub 1R}) also differed from that of 5-HT{sub 1B} and 5-HT{sub 2} sites, but was similar to that of the 5-HT{sub 1D} site. However, significant differences existed between the 5-HT{sub 1D} and 5-HT{sub 1B} sites for their K{sub i} values for spiperone, spirilene, metergoline, and methiothepin. The study of modulatory agents also showed differences between the 5-HT{sub 1R} and 5-HT{sub 1D} sites. In addition, calcium enhanced the effects of GTP on the 5-HT{sub 1R} sites, whereas calcium inhibited the GTP effect on the 5-HT{sub 1D} sites.
Country of PublicationUnited States
LanguageEnglish
FormatMedium: X; Size: Pages: 1433-1442
System Entry Date2008 Feb 06

Top