Bibliographic Citation
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| Title | CARTESIAN DIVER RESPIRATOR FOR THE STUDY OF OXYGEN UPTAKE OF YEAST CELLS IRRADIATED BY X-RAYS |
| Creator/Author | Wai, N. ; Lo, S. |
| Publication Date | 1963 Jan 01 |
| OSTI Identifier | OSTI ID: 4672342 |
| Resource Type | Journal Article |
| Resource Relation | Journal Name: Bull. Inst. Chem. Acad. Sinica.; Journal Volume: Vol: No. 7; Other Information: Orig. Receipt Date: 31-DEC-63 |
| Research Org | Originating Research Org. not identified |
| Subject | BIOLOGY AND MEDICINE; CYTOLOGY; EQUATIONS; ERRORS; MANOMETERS; METABOLISM; OXIDATION; OXYGEN; PREPARATION; PRESSURE; RADIATION INJURIES; RADIOSENSITIVITY; REDUCTION; TEMPERATURE; X RADIATION; YEASTS |
| Description/Abstract | Respiration of a small number of cells may be demonstrated by the use of the Cartesian diver respirometer with an inclined manometer tube. The principle of the diver is simple and based on the compound density equation, but the techniques for preparing, filling, and immersing the diver require much experience. An equation was derived for the calculation of O/sub 2. uptake. From the experimental results, it was found that the intact yeast cells were sensitive to x radiation. The O/sub 2/ uptake decrease might be used for the indication of the degree of damage incurred by the cells. When the dosage did not exceed the tolerance limit of the cell, there were various grades of radioinduced damage within the cell. The influence of temperature and pressure on the measuring system was quite evident. In order to compensate the experimental error, it was recommended to run a blank diver with another set of inclined manometer. The Cartesian diver, when used as a respirometer, is somewhat superior to the Warburg manometer, because the respiration of a small number of cells may be demonstrated. (P.C.H.) |
| Country of Publication | Country unknown/Code not available |
| Language | English |
| Format | Medium: X; Size: Pages: 1-25 |
| System Entry Date | 2009 Dec 15 |
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