THE INFLUENCE OF RADIOMIMETIC SUBSTANCES ON DEOXYRIBONUCLEIC ACID SYNTHESIS AND FUNCTION STUDIED IN ESCHERICHIA COLI/PHAGE SYSTEMS. VI. THE ACTION OF Di(2-CHLOROETHYL)METHYLAMINE (HN2) ON BACTERIOPHAGE T2 DURING THE INTRACELLULAR GROWTH CYCLE
Studies were made on the effects of di(2-chloroethyl)methylamine on bacteriophage T2 and E. coli strains B and B/r. The procedures adopted and the results afforded are reported. Five main conclusions were drawn: immediately after infection of the cell the phage material offers a more resistant target to HN2 than does the extracellular phage; the first 2 or 3 min after infection there is a small increase in sensitivity; a striking increase in resistance occurs around the 8th min together with a change in form of the survival curves to a multi-hit type; thereafter, though the curves remain multi-hit a second increase in sensitivity is manifested; there is a component of the infected cell population that shows a high degree of resistance at all times. The first two results are attributed to changes in the geometrical form of the phage deoxyribonucleic acid (DNA) consequent upon its extrusion into the cell from the phage head; it is also considered that the early transient increase in sensitivity might be associated with the formation of a DNA--RNA complex. The third result is attributed to DNA replication, which is known to occur at this time, thus providing a multiplicity of target, though it is not known if multiplicity reactivation takes place. The fourth result is thought likely to be the consequence of the condensation of phage DNA into discrete units having the same geometry as that of the fully formed phage. No explanation is currently offered for the occurrence of highly resistant complexes. (P.C.H.)
- Research Organization:
- Royal Cancer Hospital, London
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-18-008214
- OSTI ID:
- 4113170
- Journal Information:
- Proc. Roy. Soc. (London), Ser. B, Vol. Vol: 159; Other Information: Orig. Receipt Date: 31-DEC-64
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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