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We investigated the interaction of dissolved actinides Th, U, Np Zgpu, and Am,
with a pure and a mixed culture of halophilic bactezia isolated from the Waste Isolation H.Iot
Plant repository under anaerobic conditions to evaluate their potentiaI transport as
biocolloids from the waste site. The sizes of the bacterial cells studied ranged from ().54 x
0.48 pm to 7.7 x 0.67pm Using sequential mimofiltration, we determined the ~~ation
of actinides with fi-ee-living (mobile) bacterial cells suspended in a fluid medium containing.
NaCl or M=W12 brine, at various phaes of their growth cycIes. The number of suspended
kcteria rangy-d born 106 to 109 cells ml-*. Tine amount of actinide associatd with the
wspend~ cell fraction (cakzdated & mol cell-*) was very Iow: Th, 10-*2; U, 10-1s - 10-lS; - ~
Np, 1o-15- 10-19; Pu, 10-ls -10-21 ; and h, 10-1* - 10-*9 ; and it varied with the bacteihl
-
CUIture studied. l%e differe&es in the asswiation are amibuted to the extent of
bioamxmdation and biosorption by the bacteria pH, the compo&on of the brine, and the
speziation and bioavaiIability of the actinides.
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| Authors: |
Dodge, C.J.;
Dunn, M.;
Francis, A.J.;
Gillow, J.B.;
Mantione, K.;
Pansoy-Hjelvik, M.E.;
Papenguth, H.W.;
Strietelmeier, B.A.
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| Publication Date: | 1998 Dec 17 |
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| OSTI Identifier: | OSTI ID: 2439; Legacy ID: DE00002439 |
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| Report Number(s): | SAND98-0370J |
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| DOE Contract Number: | AC04-94AL85000 |
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| Resource Type: | Journal Article |
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| Resource Relation: | Journal Name: Radiochemeca Acta |
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| Research Org: | Sandia National Laboratories, Albuquerque, NM, and Livermore, CA |
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| Sponsoring Org: | USDOE |
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| Country of Publication: | United States |
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| Language: | English |
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| Other Number(s): | Other: ON: DE00002439 |
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| Subject: | 05 NUCLEAR FUELS; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; Microorganisms; Microorganisms; Actinides; Actinides; Radioactive Wastes; Radioactive Wastes |
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| Update Date: | 2009 Dec 16 |
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