Title: Targeted polypeptide degradation

Abstract

This invention pertains to compositions, methods, cells and organisms useful for selectively localizing polypeptides to the proteasome for degradation. Therapeutic methods and pharmaceutical compositions for treating disorders associated with the expression and/or activity of a polypeptide by targeting these polypeptides for degradation, as well as methods for targeting therapeutic polypeptides for degradation and/or activating therapeutic polypeptides by degradation are provided. The invention provides methods for identifying compounds that mediate proteasome localization and/or polypeptide degradation. The invention also provides research tools for the study of protein function.

Inventors:

Church, George M ^[1]; Janse, Daniel M ^[1]

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+ Show Inventor Affiliations

1. Brookline, MA

Issue Date:

Tue May 13 00:00:00 EDT 2008

Research Org.:

Harvard University

Sponsoring Org.:

USDOE

OSTI Identifier:

983006

Patent Number(s):

7371539

Application Number:

11/003,103

Assignee:

President and Fellows of Harvard College (Cambridge, MA)

Patent Classifications (CPCs):

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K2319/20 - containing a tag with affinity for a non-protein ligand
C07K2319/95 - containing a motif/fusion for degradation

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/62 - DNA sequences coding for fusion proteins

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N2500/02 - Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B

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DOE Contract Number:  

FG02-87ER60565

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES