Cellobiohydrolase I gene and improved variants
Abstract
The disclosure provides a method for preparing an active exoglucanase in a heterologous host of eukaryotic origin. The method includes mutagenesis to reduce glycosylation of the exoglucanase when expressed in a heterologous host. It is further disclosed a method to produce variant cellobiohydrolase that is stable at high temperature through mutagenesis.
- Inventors:
-
- Golden, CO
- Berthoud, CO
- San Carlos, CA
- Lakewood, CO
- Littleton, CO
- Issue Date:
- Research Org.:
- National Energy Technology Laboratory (NETL), Pittsburgh, PA, Morgantown, WV (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 982999
- Patent Number(s):
- 7375197
- Application Number:
- 10/031,496
- Assignee:
- MidWest Research Institute (Kansas City, MO)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
- DOE Contract Number:
- AC36-99G0-10337
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Adney, William S, Decker, Stephen R, Mc Carter, Suzanne, Baker, John O, Nieves, Raphael, Himmel, Michael E, and Vinzant, Todd B. Cellobiohydrolase I gene and improved variants. United States: N. p., 2008.
Web.
Adney, William S, Decker, Stephen R, Mc Carter, Suzanne, Baker, John O, Nieves, Raphael, Himmel, Michael E, & Vinzant, Todd B. Cellobiohydrolase I gene and improved variants. United States.
Adney, William S, Decker, Stephen R, Mc Carter, Suzanne, Baker, John O, Nieves, Raphael, Himmel, Michael E, and Vinzant, Todd B. Tue .
"Cellobiohydrolase I gene and improved variants". United States. https://www.osti.gov/servlets/purl/982999.
@article{osti_982999,
title = {Cellobiohydrolase I gene and improved variants},
author = {Adney, William S and Decker, Stephen R and Mc Carter, Suzanne and Baker, John O and Nieves, Raphael and Himmel, Michael E and Vinzant, Todd B},
abstractNote = {The disclosure provides a method for preparing an active exoglucanase in a heterologous host of eukaryotic origin. The method includes mutagenesis to reduce glycosylation of the exoglucanase when expressed in a heterologous host. It is further disclosed a method to produce variant cellobiohydrolase that is stable at high temperature through mutagenesis.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 20 00:00:00 EDT 2008},
month = {Tue May 20 00:00:00 EDT 2008}
}
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Works referenced in this record:
Cloning and Expression of Trichoderma reesei Cellobiohydrolase I in Pichia pastoris
journal, October 1999
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Modified glycosylation of cellobiohydrolase I from a high cellulase-producing mutant strain of Trichoderma reesei
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In vitro Conversion of the Carbohydrate Moiety of Fungal Glycoproteins to Mammalian-type Oligosaccharides. Evidence for N -acetylglucosaminyltransferase-I-accepting Glycans from Trichoderma reesei
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