Circular permutant GFP insertion folding reporters
Abstract
Provided are methods of assaying and improving protein folding using circular permutants of fluorescent proteins, including circular permutants of GFP variants and combinations thereof. The invention further provides various nucleic acid molecules and vectors incorporating such nucleic acid molecules, comprising polynucleotides encoding fluorescent protein circular permutants derived from superfolder GFP, which polynucleotides include an internal cloning site into which a heterologous polynucleotide may be inserted in-frame with the circular permutant coding sequence, and which when expressed are capable of reporting on the degree to which a polypeptide encoded by such an inserted heterologous polynucleotide is correctly folded by correlation with the degree of fluorescence exhibited.
- Inventors:
-
- Santa Fe, NM
- Los Alamos, NM
- Issue Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 941141
- Patent Number(s):
- 7390640
- Application Number:
- 11/360,178
- Assignee:
- Los Alamos National Security, LLC (Los Alamos, NM)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- W-7405-ENG-36; FG02-98ER62647
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Waldo, Geoffrey S, and Cabantous, Stephanie. Circular permutant GFP insertion folding reporters. United States: N. p., 2008.
Web.
Waldo, Geoffrey S, & Cabantous, Stephanie. Circular permutant GFP insertion folding reporters. United States.
Waldo, Geoffrey S, and Cabantous, Stephanie. Tue .
"Circular permutant GFP insertion folding reporters". United States. https://www.osti.gov/servlets/purl/941141.
@article{osti_941141,
title = {Circular permutant GFP insertion folding reporters},
author = {Waldo, Geoffrey S and Cabantous, Stephanie},
abstractNote = {Provided are methods of assaying and improving protein folding using circular permutants of fluorescent proteins, including circular permutants of GFP variants and combinations thereof. The invention further provides various nucleic acid molecules and vectors incorporating such nucleic acid molecules, comprising polynucleotides encoding fluorescent protein circular permutants derived from superfolder GFP, which polynucleotides include an internal cloning site into which a heterologous polynucleotide may be inserted in-frame with the circular permutant coding sequence, and which when expressed are capable of reporting on the degree to which a polypeptide encoded by such an inserted heterologous polynucleotide is correctly folded by correlation with the degree of fluorescence exhibited.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jun 24 00:00:00 EDT 2008},
month = {Tue Jun 24 00:00:00 EDT 2008}
}
Works referenced in this record:
Testing the role of chain connectivity on the stability and structure of dihydrofolate reductase from E. coli : Fragment complementation and circular permutation reveal stable, alternatively folded forms
journal, January 2001
- Smith, Virginia F.; Matthews, C. Robert
- Protein Science, Vol. 10, Issue 1