Nested methylation-specific polymerase chain reaction cancer detection method
Abstract
A molecular marker-based method for monitoring and detecting cancer in humans. Aberrant methylation of gene promoters is a marker for cancer risk in humans. A two-stage, or "nested" polymerase chain reaction method is disclosed for detecting methylated DNA sequences at sufficiently high levels of sensitivity to permit cancer screening in biological fluid samples, such as sputum, obtained non-invasively. The method is for detecting the aberrant methylation of the p16 gene, O 6-methylguanine-DNA methyltransferase gene, Death-associated protein kinase gene, RAS-associated family 1 gene, or other gene promoters. The method offers a potentially powerful approach to population-based screening for the detection of lung and other cancers.
- Inventors:
-
- Albuquerque, NM
- Edgewood, NM
- Issue Date:
- Research Org.:
- Lovelace Respiratory Research Institute (Albuquerque, NM); St Mary's Hospital and Medical Center
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 909343
- Patent Number(s):
- 7214485
- Application Number:
- 10/344,815
- Assignee:
- Lovelace Respiratory Research Institute (Albuquerque, NM)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FC04-96AL76406; FG02-90ER60939
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
Belinsky, Steven A, and Palmisano, William A. Nested methylation-specific polymerase chain reaction cancer detection method. United States: N. p., 2007.
Web.
Belinsky, Steven A, & Palmisano, William A. Nested methylation-specific polymerase chain reaction cancer detection method. United States.
Belinsky, Steven A, and Palmisano, William A. Tue .
"Nested methylation-specific polymerase chain reaction cancer detection method". United States. https://www.osti.gov/servlets/purl/909343.
@article{osti_909343,
title = {Nested methylation-specific polymerase chain reaction cancer detection method},
author = {Belinsky, Steven A and Palmisano, William A},
abstractNote = {A molecular marker-based method for monitoring and detecting cancer in humans. Aberrant methylation of gene promoters is a marker for cancer risk in humans. A two-stage, or "nested" polymerase chain reaction method is disclosed for detecting methylated DNA sequences at sufficiently high levels of sensitivity to permit cancer screening in biological fluid samples, such as sputum, obtained non-invasively. The method is for detecting the aberrant methylation of the p16 gene, O 6-methylguanine-DNA methyltransferase gene, Death-associated protein kinase gene, RAS-associated family 1 gene, or other gene promoters. The method offers a potentially powerful approach to population-based screening for the detection of lung and other cancers.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 08 00:00:00 EDT 2007},
month = {Tue May 08 00:00:00 EDT 2007}
}
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