Methods And Devices For Characterizing Duplex Nucleic Acid Molecules
Abstract
Methods and devices are provided for characterizing a duplex nucleic acid, e.g., a duplex DNA molecule. In the subject methods, a fluid conducting medium that includes a duplex nucleic acid molecule is contacted with a nanopore under the influence of an applied electric field and the resulting changes in current through the nanopore caused by the duplex nucleic acid molecule are monitored. The observed changes in current through the nanopore are then employed as a set of data values to characterize the duplex nucleic acid, where the set of data values may be employed in raw form or manipulated, e.g., into a current blockade profile. Also provided are nanopore devices for practicing the subject methods, where the subject nanopore devices are characterized by the presence of an algorithm which directs a processing means to employ monitored changes in current through a nanopore to characterize a duplex nucleic acid molecule responsible for the current changes. The subject methods and devices find use in a variety of applications, including, among other applications, the identification of an analyte duplex DNA molecule in a sample, the specific base sequence at a single nulceotide polymorphism (SNP), and the sequencing of duplex DNA molecules.
- Inventors:
-
- Santa Cruz, CA
- Issue Date:
- Research Org.:
- Univ. of California (United States)
- OSTI Identifier:
- 879731
- Patent Number(s):
- 6936433
- Application Number:
- 09/990102
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- FG03-99ER62849
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Akeson, Mark, Vercoutere, Wenonah, Haussler, David, and Winters-Hilt, Stephen. Methods And Devices For Characterizing Duplex Nucleic Acid Molecules. United States: N. p., 2005.
Web.
Akeson, Mark, Vercoutere, Wenonah, Haussler, David, & Winters-Hilt, Stephen. Methods And Devices For Characterizing Duplex Nucleic Acid Molecules. United States.
Akeson, Mark, Vercoutere, Wenonah, Haussler, David, and Winters-Hilt, Stephen. Tue .
"Methods And Devices For Characterizing Duplex Nucleic Acid Molecules". United States. https://www.osti.gov/servlets/purl/879731.
@article{osti_879731,
title = {Methods And Devices For Characterizing Duplex Nucleic Acid Molecules},
author = {Akeson, Mark and Vercoutere, Wenonah and Haussler, David and Winters-Hilt, Stephen},
abstractNote = {Methods and devices are provided for characterizing a duplex nucleic acid, e.g., a duplex DNA molecule. In the subject methods, a fluid conducting medium that includes a duplex nucleic acid molecule is contacted with a nanopore under the influence of an applied electric field and the resulting changes in current through the nanopore caused by the duplex nucleic acid molecule are monitored. The observed changes in current through the nanopore are then employed as a set of data values to characterize the duplex nucleic acid, where the set of data values may be employed in raw form or manipulated, e.g., into a current blockade profile. Also provided are nanopore devices for practicing the subject methods, where the subject nanopore devices are characterized by the presence of an algorithm which directs a processing means to employ monitored changes in current through a nanopore to characterize a duplex nucleic acid molecule responsible for the current changes. The subject methods and devices find use in a variety of applications, including, among other applications, the identification of an analyte duplex DNA molecule in a sample, the specific base sequence at a single nulceotide polymorphism (SNP), and the sequencing of duplex DNA molecules.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Aug 30 00:00:00 EDT 2005},
month = {Tue Aug 30 00:00:00 EDT 2005}
}
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