Copolymers for capillary gel electrophoresis
Abstract
This invention relates to an electrophoresis separation medium having a gel matrix of at least one random, linear copolymer comprising a primary comonomer and at least one secondary comonomer, wherein the comonomers are randomly distributed along the copolymer chain. The primary comonomer is an acrylamide or an acrylamide derivative that provides the primary physical, chemical, and sieving properties of the gel matrix. The at least one secondary comonomer imparts an inherent physical, chemical, or sieving property to the copolymer chain. The primary and secondary comonomers are present in a ratio sufficient to induce desired properties that optimize electrophoresis performance. The invention also relates to a method of separating a mixture of biological molecules using this gel matrix, a method of preparing the novel electrophoresis separation medium, and a capillary tube filled with the electrophoresis separation medium.
- Inventors:
- Issue Date:
- Research Org.:
- SpectruMedix LLC, State College, PA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 879705
- Patent Number(s):
- 6926815
- Application Number:
- 09/843,918
- Assignee:
- SpectruMedix LLC (State College, PA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- FG02-97ER82461
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2001 Apr 30
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Liu, Changsheng, and Li, Qingbo. Copolymers for capillary gel electrophoresis. United States: N. p., 2005.
Web.
Liu, Changsheng, & Li, Qingbo. Copolymers for capillary gel electrophoresis. United States.
Liu, Changsheng, and Li, Qingbo. Tue .
"Copolymers for capillary gel electrophoresis". United States. https://www.osti.gov/servlets/purl/879705.
@article{osti_879705,
title = {Copolymers for capillary gel electrophoresis},
author = {Liu, Changsheng and Li, Qingbo},
abstractNote = {This invention relates to an electrophoresis separation medium having a gel matrix of at least one random, linear copolymer comprising a primary comonomer and at least one secondary comonomer, wherein the comonomers are randomly distributed along the copolymer chain. The primary comonomer is an acrylamide or an acrylamide derivative that provides the primary physical, chemical, and sieving properties of the gel matrix. The at least one secondary comonomer imparts an inherent physical, chemical, or sieving property to the copolymer chain. The primary and secondary comonomers are present in a ratio sufficient to induce desired properties that optimize electrophoresis performance. The invention also relates to a method of separating a mixture of biological molecules using this gel matrix, a method of preparing the novel electrophoresis separation medium, and a capillary tube filled with the electrophoresis separation medium.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Aug 09 00:00:00 EDT 2005},
month = {Tue Aug 09 00:00:00 EDT 2005}
}
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