Combinatorial Screening Of Inorganic And Organometallic Materials
Abstract
A method for differentiating and enumerating nucleated red blood cells in a blood sample is described. The method includes the steps of lysing red blood cells of a blood sample with a lytic reagent, measuring nucleated blood cells by DC impedance measurement in a non-focused flow aperture, differentiating nucleated red blood cells from other cell types, and reporting nucleated red blood cells in the blood sample. The method further includes subtracting nucleated red blood cells and other interference materials from the count of remaining blood cells, and reporting a corrected white blood cell count of the blood sample. Additionally, the method further includes measuring spectrophotometric absorbance of the sample mixture at a predetermined wavelength of a hemoglobin chromogen formed upon lysing the blood sample, and reporting hemoglobin concentration of the blood sample.
- Inventors:
-
- (Miami, FL), Li, Jing (Miami, FL), Britton, Ted W. (Sunrise, FL)
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- OSTI Identifier:
- 879545
- Patent Number(s):
- 6410330
- Application Number:
- 09/122932
- Assignee:
- Coulter International Corp. (Miami, FL)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- DOE Contract Number:
- AC03-76SF00098
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Li, Yi. Combinatorial Screening Of Inorganic And Organometallic Materials. United States: N. p., 2002.
Web.
Li, Yi. Combinatorial Screening Of Inorganic And Organometallic Materials. United States.
Li, Yi. Tue .
"Combinatorial Screening Of Inorganic And Organometallic Materials". United States. https://www.osti.gov/servlets/purl/879545.
@article{osti_879545,
title = {Combinatorial Screening Of Inorganic And Organometallic Materials},
author = {Li, Yi},
abstractNote = {A method for differentiating and enumerating nucleated red blood cells in a blood sample is described. The method includes the steps of lysing red blood cells of a blood sample with a lytic reagent, measuring nucleated blood cells by DC impedance measurement in a non-focused flow aperture, differentiating nucleated red blood cells from other cell types, and reporting nucleated red blood cells in the blood sample. The method further includes subtracting nucleated red blood cells and other interference materials from the count of remaining blood cells, and reporting a corrected white blood cell count of the blood sample. Additionally, the method further includes measuring spectrophotometric absorbance of the sample mixture at a predetermined wavelength of a hemoglobin chromogen formed upon lysing the blood sample, and reporting hemoglobin concentration of the blood sample.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jun 25 00:00:00 EDT 2002},
month = {Tue Jun 25 00:00:00 EDT 2002}
}