Chromosome-Specific Staining To Detect Genetic Rearrangements Associated With Chromosome 3 And/Or Chromosone 17
Abstract
Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML), retinoblastoma, ovarian and uterine cancers, and for biological dosimetry. Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar but genetically different diseases, and for many prognostic and diagnostic applications.
- Inventors:
-
- Livermore, CA
- Walnut Creek, CA
- Tampere, FI
- Tokyo, JP
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- OSTI Identifier:
- 879286
- Patent Number(s):
- 6344315
- Application Number:
- 08/477316
- Assignee:
- The Regents of The University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Gray, Joe W., Pinkel, Daniel, Kallioniemi, Olli-Pekka, Kallioniemi, Anne, and Sakamoto, Masaru. Chromosome-Specific Staining To Detect Genetic Rearrangements Associated With Chromosome 3 And/Or Chromosone 17. United States: N. p., 2002.
Web.
Gray, Joe W., Pinkel, Daniel, Kallioniemi, Olli-Pekka, Kallioniemi, Anne, & Sakamoto, Masaru. Chromosome-Specific Staining To Detect Genetic Rearrangements Associated With Chromosome 3 And/Or Chromosone 17. United States.
Gray, Joe W., Pinkel, Daniel, Kallioniemi, Olli-Pekka, Kallioniemi, Anne, and Sakamoto, Masaru. Tue .
"Chromosome-Specific Staining To Detect Genetic Rearrangements Associated With Chromosome 3 And/Or Chromosone 17". United States. https://www.osti.gov/servlets/purl/879286.
@article{osti_879286,
title = {Chromosome-Specific Staining To Detect Genetic Rearrangements Associated With Chromosome 3 And/Or Chromosone 17},
author = {Gray, Joe W. and Pinkel, Daniel and Kallioniemi, Olli-Pekka and Kallioniemi, Anne and Sakamoto, Masaru},
abstractNote = {Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML), retinoblastoma, ovarian and uterine cancers, and for biological dosimetry. Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar but genetically different diseases, and for many prognostic and diagnostic applications.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Feb 05 00:00:00 EST 2002},
month = {Tue Feb 05 00:00:00 EST 2002}
}
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Works referenced in this record:
Hereditary spherocytosis associated with deletion of human erythrocyte ankyrin gene on chromosome 8
journal, June 1990
- Lux, Samuel E.; Tse, William T.; Menninger, Joan C.
- Nature, Vol. 345, Issue 6277
Direct carrier detection by in situ suppression hybridization with cosmid clones of the Duchenne/Becker muscular dystrophy locus
journal, October 1990
- Ried, T.; Mahler, V.; Vogt, P.
- Human Genetics, Vol. 85, Issue 6
Preparative dual-beam sorting of the human Y chromosome and in situ hybridization of cloned DNA probes
journal, November 1984
- Cremer, C.; Rappold, G.; Gray, J. W.
- Cytometry, Vol. 5, Issue 6
Two homoeo box loci mapped in evolutionarily related mouse and human chromosomes
journal, March 1985
- Rabin, Mark; Hart, Charles P.; Ferguson-Smith, Anne
- Nature, Vol. 314, Issue 6007
[38] Nucleic acid hybridization in cytological preparations
book, January 1971
- Gall, Joseph G.; Pardue, Mary Lou
- Methods in Enzymology
Chromosomal localization of a single copy gene by in situ hybridization - human ? globin genes on the short arm of chromosome 11
journal, May 1981
- Malcolm, S.; Barton, P.; Murphy, C.
- Annals of Human Genetics, Vol. 45, Issue 2
Four restriction fragment length polymorphisms revealed by probes from a single cosmid map to human chromosome 12q
journal, January 1986
- Buroker, N. E.; Magenis, R. E.; Weliky, K.
- Human Genetics, Vol. 72, Issue 1
The application of DNA recombinant technology to the analysis of the human genome and genetic disease
journal, October 1981
- Davies, Kay E.
- Human Genetics, Vol. 58, Issue 4
Multiple sex chromosomes in Drosophila miranda: A system to study the degeneration of a chromosome
journal, September 1982
- Steinemann, M.
- Chromosoma, Vol. 86, Issue 1
Quantitative in situ hybridization of ribosomal RNA species to polytene chromosomes of Drosophila melanogaster
journal, September 1977
- Szabo, Paul; Elder, Robert; Steffensen, Dale M.
- Journal of Molecular Biology, Vol. 115, Issue 3
Chemical synthesis of oligonucleotides containing a free sulphydryl group and subsequent attachment of thiol specific probes
journal, January 1985
- Connolly, Bernard A.; Rider, Peter
- Nucleic Acids Research, Vol. 13, Issue 12
Optimizing comparative genomic hybridization for analysis of DNA sequence copy number changes in solid tumors
journal, August 1994
- Kallioniemi, Olli-P.; Kallioniemi, Anne; Piper, Jim
- Genes, Chromosomes and Cancer, Vol. 10, Issue 4
ERBB2 amplification in breast cancer analyzed by fluorescence in situ hybridization.
journal, June 1992
- Kallioniemi, O. P.; Kallioniemi, A.; Kurisu, W.
- Proceedings of the National Academy of Sciences, Vol. 89, Issue 12
Analysis of a dispersed repetitive DNA sequence in isogenic lines of Drosophila
journal, May 1981
- Pierce, Dorothy A.; Lucchesi, John C.
- Chromosoma, Vol. 82, Issue 4
Cytogenetics of the parthenogenetic grasshopper Warramaba virgo and its bisexual relatives: VII. Evidence from repeated DNA sequences for a dual origin of W. virgo
journal, May 1981
- Dennis, E. S.; Peacock, W. J.; White, M. J. D.
- Chromosoma, Vol. 82, Issue 4
Cytological Hybridization to Mammalian Chromosomes
book, January 1982
- Hendkrson, Ann S.
- International Review of Cytology Volume 76
Translocation of c-myc in the hereditary renal cell carcinoma associated with a t(3;8)(p14.2;q24.13) chromosomal translocation.
journal, October 1985
- Drabkin, H. A.; Bradley, C.; Hart, I.
- Proceedings of the National Academy of Sciences, Vol. 82, Issue 20
Quantitative in situ hybridization reveals extent of sequence homology between related DNA sequences in Drosophila melanogaster
journal, July 1981
- Brock, Hugh W.; Roberts, David B.
- Chromosoma, Vol. 83, Issue 2
Characterization of chromosomal anomalies in human breast cancer
journal, October 1990
- Dutrillaux, Bernard; Gerbault-Seureau, Michele; Zafrani, Brigitte
- Cancer Genetics and Cytogenetics, Vol. 49, Issue 2
The Use of the Avidin-Biotin Complex as a Tool in Molecular Biology
book, February 1980
- Bayer, Edward A.; Wilchek, Meir
- Methods of Biochemical Analysis, Volume 26
Translocation of the p53 gene in t(15;17) in acute promyelocytic leukaemia
journal, August 1985
- Le Beau, Michelle M.; Westbrook, Carol A.; Diaz, Manuel O.
- Nature, Vol. 316, Issue 6031
The isolation and characterization of linked δ- and β-globin genes from a cloned library of human DNA
journal, December 1978
- Lawn, Richard M.; Fritsch, Edward F.; Parker, Richard C.
- Cell, Vol. 15, Issue 4
Fine mapping of the Huntington disease linked D4S10 locus by non-radioactive in situ hybridization
journal, August 1986
- Landegent, J. E.; Jansen in de Wal, N.; Fisser-Groen, Y. M.
- Human Genetics, Vol. 73, Issue 4
Organization and heterogeneity of sequences within a repeating unit of human Y chromosome deoxyribonucleic acid
journal, July 1979
- Kunkel, Louis M.; Smith, Kirby D.; Boyer, Samuel H.
- Biochemistry, Vol. 18, Issue 15
In Situ Hybridization to Cellular RNAs
book, January 1985
- Angerer, R. C.; Cox, K. H.; Angerer, L. M.
- Genetic Engineering: Principles and Methods
Proto-oncogene amplification and homogeneously staining regions in human breast carcinomas
journal, May 1990
- Saint-Ruf, Claude; Gerbault-Seureau, Michéle; Viegas-Péquignot, Evani
- Genes, Chromosomes and Cancer, Vol. 2, Issue 1
The activity of two heat shock loci of Drosophila hydei in tissue culture cells and salivary gland cells as analyzed by in situ hybridization of complementary DNA
journal, January 1979
- Sondermeijer, P. J. A.; Lubsen, N. H.
- Chromosoma, Vol. 72, Issue 3
Electron microscopic visualization of tRNA genes with ferritin-avidin: biotin labels
journal, January 1978
- Broker, Thomas R.; Angerer, Lynne M.; Yen, Pauline H.
- Nucleic Acids Research, Vol. 5, Issue 2
A highly polymorphic locus on chromosome 16q revealed by a probe from a chromosome-specific cosmid library
journal, December 1986
- Bufton, L.; Mohandas, T. K.; Magenis, R. E.
- Human Genetics, Vol. 74, Issue 4
The synthesis of oligonucleotides containing an aliphatic amino group at the 5′ terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis
journal, January 1985
- Smith, Lloyd M.; Fung, Steven; Hunkapiller, Michael W.
- Nucleic Acids Research, Vol. 13, Issue 7
Amplified sequences from chromosome 15, including centromeres, nucleolar organizer regions, and centromeric heterochromatin, in homogeneously staining regions in the human melanoma cell line MeWo
journal, January 1985
- Holden, J. J. A.; Reimer, D. L.; Higgins, M. J.
- Cancer Genetics and Cytogenetics, Vol. 14, Issue 1-2
Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors
journal, October 1992
- Kallioniemi, A.; Kallioniemi, O.; Sudar, D.
- Science, Vol. 258, Issue 5083
Simultaneous visualization of seven different DNA probes by in situ hybridization using combinatorial fluorescence and digital imaging microscopy.
journal, February 1992
- Ried, T.; Baldini, A.; Rand, T. C.
- Proceedings of the National Academy of Sciences, Vol. 89, Issue 4
Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes
journal, April 1983
- Brigati, David J.; Myerson, David; Leary, Jeffry J.
- Virology, Vol. 126, Issue 1
Detection of two viral genomes in single cells by double-label hybridization in situ and color microradioautography
journal, January 1985
- Haase, A.; Walker, D.; Stowring, L.
- Science, Vol. 227, Issue 4683
Human chromosome-specific repetitive DNA sequences: novel markers for genetic analysis
journal, October 1987
- Moyzis, R. K.; Albright, K. L.; Bartholdi, M. F.
- Chromosoma, Vol. 95, Issue 6
Chemically modified nucleic acids as immunodetectable probes in hybridization experiments.
journal, June 1984
- Tchen, P.; Fuchs, R. P.; Sage, E.
- Proceedings of the National Academy of Sciences, Vol. 81, Issue 11
Localization of a unique gene by direct hybridization in situ.
journal, June 1981
- Gerhard, D. S.; Kawasaki, E. S.; Bancroft, F. C.
- Proceedings of the National Academy of Sciences, Vol. 78, Issue 6
Quantitation of in situ hybridization of ribosomal ribonucleic acids to human diploid cells
journal, October 1980
- Cot�, Babette D.; Uhlenbeck, Olke C.; Steffensen, Dale M.
- Chromosoma, Vol. 80, Issue 3
Organization and chromosomal specificity of autosomal homologs of human Y chromosome repeated DNA
journal, January 1985
- Burk, Robert D.; Szabo, Paul; O'Brien, Steve
- Chromosoma, Vol. 92, Issue 3
A polymorphic locus on the long arm of chromosome 20 defined by two probes from a single cosmid
journal, August 1986
- Litt, M.; Sheehy, R.; Bruns, G. A.
- Human Genetics, Vol. 73, Issue 4
A new era in mammalian gene mapping: somatic cell genetics and recombinant DNA methodologies
journal, November 1981
- Ruddle, F. H.
- Nature, Vol. 294, Issue 5837
Biotin and fluorescent labeling of RNA using T4 RNA ligase
journal, January 1983
- Richardson, Ross W.; Gumport, Richard I.
- Nucleic Acids Research, Vol. 11, Issue 18
Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes
journal, January 1986
- Fuscoe, J. C.; Clark, L. M.; Van Dilla, M. A.
- Cytogenetic and Genome Research, Vol. 43, Issue 1-2
Differential distribution of long and short interspersed element sequences in the mouse genome: chromosome karyotyping by fluorescence in situ hybridization.
journal, October 1990
- Boyle, A. L.; Ballard, S. G.; Ward, D. C.
- Proceedings of the National Academy of Sciences, Vol. 87, Issue 19
Construction and characterization of genomic libraries from specific human chromosomes.
journal, May 1982
- Krumlauf, R.; Jeanpierre, M.; Young, B. D.
- Proceedings of the National Academy of Sciences, Vol. 79, Issue 9
A procedure for the isolation of deoxyribonucleic acid from micro-organisms
journal, April 1961
- Marmur, J.
- Journal of Molecular Biology, Vol. 3, Issue 2
Fluorescence in situ hybridization with Alu and L1 polymerase chain reaction probes for rapid characterization of human chromosomes in hybrid cell lines.
journal, September 1990
- Lichter, P.; Ledbetter, S. A.; Ledbetter, D. H.
- Proceedings of the National Academy of Sciences, Vol. 87, Issue 17
A new method for fluorescence microscopical localization of specific DNA sequences by in situ hybridization of fluorochrome-labelled RNA
journal, August 1980
- Bauman, J. G. J.; Wiegant, J.; Borst, P.
- Experimental Cell Research, Vol. 128, Issue 2
Genomic mismatch scanning: a new approach to genetic linkage mapping
journal, May 1993
- Nelson, Stanley F.; McCusker, John H.; Sander, Mark A.
- Nature Genetics, Vol. 4, Issue 1