Multiple tag labeling method for DNA sequencing
Abstract
A DNA sequencing method is described which uses single lane or channel electrophoresis. Sequencing fragments are separated in the lane and detected using a laser-excited, confocal fluorescence scanner. Each set of DNA sequencing fragments is separated in the same lane and then distinguished using a binary coding scheme employing only two different fluorescent labels. Also described is a method of using radioisotope labels. 5 figs.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of California (United States)
- OSTI Identifier:
- 87755
- Patent Number(s):
- 5436130
- Application Number:
- PAN: 8-143,377
- Assignee:
- Univ. of California, Oakland, CA (United States)
- DOE Contract Number:
- FG03-91ER61125
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 25 Jul 1995
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA SEQUENCERS; DESIGN; DNA SEQUENCING; TRACER TECHNIQUES; RADIOISOTOPES; LABELLING
Citation Formats
Mathies, R A, Huang, X C, and Quesada, M A. Multiple tag labeling method for DNA sequencing. United States: N. p., 1995.
Web.
Mathies, R A, Huang, X C, & Quesada, M A. Multiple tag labeling method for DNA sequencing. United States.
Mathies, R A, Huang, X C, and Quesada, M A. Tue .
"Multiple tag labeling method for DNA sequencing". United States.
@article{osti_87755,
title = {Multiple tag labeling method for DNA sequencing},
author = {Mathies, R A and Huang, X C and Quesada, M A},
abstractNote = {A DNA sequencing method is described which uses single lane or channel electrophoresis. Sequencing fragments are separated in the lane and detected using a laser-excited, confocal fluorescence scanner. Each set of DNA sequencing fragments is separated in the same lane and then distinguished using a binary coding scheme employing only two different fluorescent labels. Also described is a method of using radioisotope labels. 5 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jul 25 00:00:00 EDT 1995},
month = {Tue Jul 25 00:00:00 EDT 1995}
}
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