Microfabricated electrochemiluminescence cell for chemical reaction detection
Abstract
A detector cell for a silicon-based or non-silicon-based sleeve type chemical reaction chamber that combines heaters, such as doped polysilicon for heating, and bulk silicon for convection cooling. The detector cell is an electrochemiluminescence cell constructed of layers of silicon with a cover layer of glass, with spaced electrodes located intermediate various layers forming the cell. The cell includes a cavity formed therein and fluid inlets for directing reaction fluid therein. The reaction chamber and detector cell may be utilized in any chemical reaction system for synthesis or processing of organic, inorganic, or biochemical reactions, such as the polymerase chain reaction (PCR) and/or other DNA reactions, such as the ligase chain reaction, which are examples of a synthetic, thermal-cycling-based reaction. The ECL cell may also be used in synthesis instruments, particularly those for DNA amplification and synthesis.
- Inventors:
-
- Berkeley, CA
- Davis, CA
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- OSTI Identifier:
- 875055
- Patent Number(s):
- 6521181
- Assignee:
- The Regents of the University of Calfornia (Oakland, CA)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- microfabricated; electrochemiluminescence; cell; chemical; reaction; detection; detector; silicon-based; non-silicon-based; sleeve; type; chamber; combines; heaters; doped; polysilicon; heating; bulk; silicon; convection; cooling; constructed; layers; cover; layer; glass; spaced; electrodes; located; intermediate; forming; cavity; formed; fluid; inlets; directing; utilized; synthesis; processing; organic; inorganic; biochemical; reactions; polymerase; chain; pcr; andor; dna; ligase; examples; synthetic; thermal-cycling-based; ecl; instruments; amplification; reaction chamber; chemical reaction; bulk silicon; detector cell; /422/435/
Citation Formats
Northrup, M Allen, Hsueh, Yun-Tai, and Smith, Rosemary L. Microfabricated electrochemiluminescence cell for chemical reaction detection. United States: N. p., 2003.
Web.
Northrup, M Allen, Hsueh, Yun-Tai, & Smith, Rosemary L. Microfabricated electrochemiluminescence cell for chemical reaction detection. United States.
Northrup, M Allen, Hsueh, Yun-Tai, and Smith, Rosemary L. Wed .
"Microfabricated electrochemiluminescence cell for chemical reaction detection". United States. https://www.osti.gov/servlets/purl/875055.
@article{osti_875055,
title = {Microfabricated electrochemiluminescence cell for chemical reaction detection},
author = {Northrup, M Allen and Hsueh, Yun-Tai and Smith, Rosemary L},
abstractNote = {A detector cell for a silicon-based or non-silicon-based sleeve type chemical reaction chamber that combines heaters, such as doped polysilicon for heating, and bulk silicon for convection cooling. The detector cell is an electrochemiluminescence cell constructed of layers of silicon with a cover layer of glass, with spaced electrodes located intermediate various layers forming the cell. The cell includes a cavity formed therein and fluid inlets for directing reaction fluid therein. The reaction chamber and detector cell may be utilized in any chemical reaction system for synthesis or processing of organic, inorganic, or biochemical reactions, such as the polymerase chain reaction (PCR) and/or other DNA reactions, such as the ligase chain reaction, which are examples of a synthetic, thermal-cycling-based reaction. The ECL cell may also be used in synthesis instruments, particularly those for DNA amplification and synthesis.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 2003},
month = {Wed Jan 01 00:00:00 EST 2003}
}
Works referenced in this record:
A microfabricated, electrochemiluminescence cell for the detection of amplified DNA
journal, July 1996
- Hsueh, Y. -T.; Smith, R. L.; Northrup, M. A.
- Sensors and Actuators B: Chemical, Vol. 33, Issue 1-3
A Mems-based Miniature DNA Analysis System
conference, January 1995
- Northrup, M. A.; Gonzalez, C.; Hadley, D.
- Proceedings of the International Solid-State Sensors and Actuators Conference - TRANSDUCERS '95