Title: Agouti polynucleotide compositions and methods of use

Abstract

Disclosed are methods and compositions comprising novel agouti polypeptides and the polynucleotides which encode them. Also disclosed are DNA segments encoding these proteins derived from human and murine cell lines, and the use of these polynucleotides and polypeptides in a variety of diagnostic and therapeutic applications. Methods, compositions, kits, and devices are also provided for identifying compounds which are inhibitors of agouti activity, and for altering fatty acid synthetase activity and intracellular calcium levels in transformed cells.

Inventors:

Woychik, Richard P ^[1]; Bultman, Scott J ^[2]; Michaud, Edward J ^[3]

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+ Show Inventor Affiliations

1. Orinda, CA
2. Lakewood, OH
3. Kingston, TN

Issue Date:

Tue Feb 04 00:00:00 EST 2003

Research Org.:

Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

OSTI Identifier:

875024

Patent Number(s):

6514747

Application Number:

09/781,811

Assignee:

UT-Battelle, LLC (Oak Ridge, TN)

Patent Classifications (CPCs):

A - HUMAN NECESSITIES A01 - AGRICULTURE A01K - ANIMAL HUSBANDRY

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

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A - HUMAN NECESSITIES A01 - AGRICULTURE A01K - ANIMAL HUSBANDRY

A01K2217/05 - Animals comprising random inserted nucleic acids
A01K2217/30 - Animal model comprising expression system for selective cell killing, e.g. toxins, enzyme dependent prodrug therapy using ganciclovir
A01K2227/105 - Murine
A01K2267/03 - Animal model, e.g. for test or diseases
A01K2267/0306 - Animal model for genetic diseases
A01K2267/0362 - Animal model for lipid/glucose metabolism, e.g. obesity, type-2 diabetes
A01K67/0275 - {Genetically modified vertebrates, e.g. transgenic}

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K14/47 - from mammals

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/8509 - {for producing genetically modified animals, e.g. transgenic}
C12N2830/008 - cell type or tissue specific enhancer/promoter combination
C12N2830/85 - mammalian
C12N2840/445 - for trans-splicing, e.g. polypyrimidine tract, branch point splicing

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Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

agouti; polynucleotide; compositions; methods; disclosed; comprising; novel; polypeptides; polynucleotides; encode; dna; segments; encoding; proteins; derived; human; murine; cell; lines; variety; diagnostic; therapeutic; applications; kits; devices; provided; identifying; compounds; inhibitors; activity; altering; fatty; acid; synthetase; intracellular; calcium; levels; transformed; cells; fatty acid; /435/999/