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Title: Rapid method to detect duplex formation in sequencing by hybridization methods, a method for constructing containment structures for reagent interaction

Abstract

A method for determining the existence of duplexes of oligonucleotide complementary molecules is provided whereby a plurality of immobilized oligonucleotide molecules, each of a specific length and each having a specific base sequence, is contacted with complementary, single stranded oligonucleotide molecules to form a duplex so as to facilitate intercalation of a fluorescent dye between the base planes of the duplex. The invention also provides for a method for constructing oligonucleotide matrices comprising confining light sensitive fluid to a surface, exposing said light-sensitive fluid to a light pattern so as to cause the fluid exposed to the light to polymerize into discrete units and adhere to the surface; and contacting each of the units with a set of different oligonucleotide molecules so as to allow the molecules to disperse into the units.

Inventors:
 [1];  [2];  [3];  [4];  [1];  [5];  [1]
+ Show Inventor Affiliations
  1. Moscow, RU
  2. Zelenograd, RU
  3. Hinsale, IL
  4. Western Springs, IL
  5. Samara, RU
Issue Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL (United States)
OSTI Identifier:
874803
Patent Number(s):
6465174
Assignee:
University of Chicago (Chicago, IL)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES
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DOE Contract Number:  
W-31109-ENG-38
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
rapid; method; detect; duplex; formation; sequencing; hybridization; methods; constructing; containment; structures; reagent; interaction; determining; existence; duplexes; oligonucleotide; complementary; molecules; provided; plurality; immobilized; specific; length; base; sequence; contacted; single; stranded; form; facilitate; intercalation; fluorescent; dye; planes; provides; matrices; comprising; confining; light; sensitive; fluid; surface; exposing; light-sensitive; pattern; exposed; polymerize; discrete; units; adhere; contacting; set; allow; disperse; base sequence; light sensitive; hybridization methods; /435/427/999/

Citation Formats

Mirzabekov, Andrei Darievich, Yershov, Gennadiy Moiseyevich, Guschin, Dmitry Yuryevich, Gemmell, Margaret Anne, Shick, Valentine V, Proudnikov, Dmitri Y, and Timofeev, Edward N. Rapid method to detect duplex formation in sequencing by hybridization methods, a method for constructing containment structures for reagent interaction. United States: N. p., 2002. Web.
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Mirzabekov, Andrei Darievich, Yershov, Gennadiy Moiseyevich, Guschin, Dmitry Yuryevich, Gemmell, Margaret Anne, Shick, Valentine V, Proudnikov, Dmitri Y, & Timofeev, Edward N. Rapid method to detect duplex formation in sequencing by hybridization methods, a method for constructing containment structures for reagent interaction. United States.
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Mirzabekov, Andrei Darievich, Yershov, Gennadiy Moiseyevich, Guschin, Dmitry Yuryevich, Gemmell, Margaret Anne, Shick, Valentine V, Proudnikov, Dmitri Y, and Timofeev, Edward N. Tue . "Rapid method to detect duplex formation in sequencing by hybridization methods, a method for constructing containment structures for reagent interaction". United States. https://www.osti.gov/servlets/purl/874803.
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@article{osti_874803,
title = {Rapid method to detect duplex formation in sequencing by hybridization methods, a method for constructing containment structures for reagent interaction},
author = {Mirzabekov, Andrei Darievich and Yershov, Gennadiy Moiseyevich and Guschin, Dmitry Yuryevich and Gemmell, Margaret Anne and Shick, Valentine V and Proudnikov, Dmitri Y and Timofeev, Edward N},
abstractNote = {A method for determining the existence of duplexes of oligonucleotide complementary molecules is provided whereby a plurality of immobilized oligonucleotide molecules, each of a specific length and each having a specific base sequence, is contacted with complementary, single stranded oligonucleotide molecules to form a duplex so as to facilitate intercalation of a fluorescent dye between the base planes of the duplex. The invention also provides for a method for constructing oligonucleotide matrices comprising confining light sensitive fluid to a surface, exposing said light-sensitive fluid to a light pattern so as to cause the fluid exposed to the light to polymerize into discrete units and adhere to the surface; and contacting each of the units with a set of different oligonucleotide molecules so as to allow the molecules to disperse into the units.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 2002},
month = {Tue Jan 01 00:00:00 EST 2002}
}
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Works referenced in this record:

Immobilization of DNA in Polyacrylamide Gel for the Manufacture of DNA and DNA–Oligonucleotide Microchips
journal, May 1998

Manual Manufacturing of Oligonucleotide, DNA, and Protein Microchips
journal, August 1997

Fabrication of Microarray of Gel-Immobilized Compounds on a Chip by Copolymerization
journal, September 1999

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