DNA attachment to support structures
Abstract
Microscopic beads or other structures are attached to nucleic acids (DNA) using a terminal transferase. The transferase adds labeled dideoxy nucleotide bases to the ends of linear strands of DNA. The labels, such as the antigens digoxigenin and biotin, bind to the antibody compounds or other appropriate complementary ligands, which are bound to the microscopic beads or other support structures. The method does not require the synthesis of a synthetic oligonucleotide probe. The method can be used to tag or label DNA even when the DNA has an unknown sequence, has blunt ends, or is a very large fragment (e.g., >500 kilobase pairs).
- Inventors:
-
- Livermore, CA
- Fresno, CA
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- OSTI Identifier:
- 874582
- Patent Number(s):
- 6420112
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- dna; attachment; support; structures; microscopic; beads; attached; nucleic; acids; terminal; transferase; adds; labeled; dideoxy; nucleotide; bases; linear; strands; labels; antigens; digoxigenin; biotin; bind; antibody; compounds; appropriate; complementary; ligands; bound; method; require; synthesis; synthetic; oligonucleotide; probe; tag; label; sequence; blunt; fragment; >500; kilobase; pairs; nucleic acid; /435/436/530/999/
Citation Formats
Balhorn, Rodney L, and Barry, Christopher H. DNA attachment to support structures. United States: N. p., 2002.
Web.
Balhorn, Rodney L, & Barry, Christopher H. DNA attachment to support structures. United States.
Balhorn, Rodney L, and Barry, Christopher H. Tue .
"DNA attachment to support structures". United States. https://www.osti.gov/servlets/purl/874582.
@article{osti_874582,
title = {DNA attachment to support structures},
author = {Balhorn, Rodney L and Barry, Christopher H},
abstractNote = {Microscopic beads or other structures are attached to nucleic acids (DNA) using a terminal transferase. The transferase adds labeled dideoxy nucleotide bases to the ends of linear strands of DNA. The labels, such as the antigens digoxigenin and biotin, bind to the antibody compounds or other appropriate complementary ligands, which are bound to the microscopic beads or other support structures. The method does not require the synthesis of a synthetic oligonucleotide probe. The method can be used to tag or label DNA even when the DNA has an unknown sequence, has blunt ends, or is a very large fragment (e.g., >500 kilobase pairs).},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 2002},
month = {Tue Jan 01 00:00:00 EST 2002}
}
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