Methods and compositions for efficient nucleic acid sequencing
Abstract
Disclosed are novel methods and compositions for rapid and highly efficient nucleic acid sequencing based upon hybridization with two sets of small oligonucleotide probes of known sequences. Extremely large nucleic acid molecules, including chromosomes and non-amplified RNA, may be sequenced without prior cloning or subcloning steps. The methods of the invention also solve various current problems associated with sequencing technology such as, for example, high noise to signal ratios and difficult discrimination, attaching many nucleic acid fragments to a surface, preparing many, longer or more complex probes and labelling more species.
- Inventors:
-
- 850 E. Greenwich Pl., Palo Alto, CA 94303
- Issue Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- OSTI Identifier:
- 874493
- Patent Number(s):
- 6401267
- Assignee:
- Drmanac; Radoje (850 E. Greenwich Pl., Palo Alto, CA 94303)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- W-31109-ENG-38
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- methods; compositions; efficient; nucleic; acid; sequencing; disclosed; novel; rapid; highly; based; hybridization; sets; oligonucleotide; probes; sequences; extremely; molecules; including; chromosomes; non-amplified; rna; sequenced; prior; cloning; subcloning; steps; solve; current; associated; technology; example; noise; signal; ratios; difficult; discrimination; attaching; fragments; surface; preparing; complex; labelling; species; nucleic acid; highly efficient; acid sequencing; oligonucleotide probe; /4/435/
Citation Formats
Drmanac, Radoje. Methods and compositions for efficient nucleic acid sequencing. United States: N. p., 2002.
Web.
Drmanac, Radoje. Methods and compositions for efficient nucleic acid sequencing. United States.
Drmanac, Radoje. Tue .
"Methods and compositions for efficient nucleic acid sequencing". United States. https://www.osti.gov/servlets/purl/874493.
@article{osti_874493,
title = {Methods and compositions for efficient nucleic acid sequencing},
author = {Drmanac, Radoje},
abstractNote = {Disclosed are novel methods and compositions for rapid and highly efficient nucleic acid sequencing based upon hybridization with two sets of small oligonucleotide probes of known sequences. Extremely large nucleic acid molecules, including chromosomes and non-amplified RNA, may be sequenced without prior cloning or subcloning steps. The methods of the invention also solve various current problems associated with sequencing technology such as, for example, high noise to signal ratios and difficult discrimination, attaching many nucleic acid fragments to a surface, preparing many, longer or more complex probes and labelling more species.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 2002},
month = {Tue Jan 01 00:00:00 EST 2002}
}
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