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Title: Geranyl diphosphate synthase large subunit, and methods of use

Abstract

A cDNA encoding geranyl diphosphate synthase large subunit from peppermint has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Replicable recombinant cloning vehicles are provided which code for geranyl diphosphate synthase large subunit). In another aspect, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding geranyl diphosphate synthase large subunit. In yet another aspect, the present invention provides isolated, recombinant geranyl diphosphate synthase protein comprising an isolated, recombinant geranyl diphosphate synthase large subunit protein and an isolated, recombinant geranyl diphosphate synthase small subunit protein. Thus, systems and methods are provided for the recombinant expression of geranyl diphosphate synthase.

Inventors:
 [1];  [2];  [3]
  1. Pullman, WA
  2. Moscow, ID
  3. Colfax, WA
Issue Date:
Research Org.:
Washington State University Research Foundation (Pullma, WA)
OSTI Identifier:
874056
Patent Number(s):
6303330
Application Number:
09/420211
Assignee:
Washington State University Research Foundation (Pullma, WA)
Patent Classifications (CPCs):
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG03-96ER20212
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
geranyl; diphosphate; synthase; subunit; methods; cdna; encoding; peppermint; isolated; sequenced; amino; acid; sequence; determined; replicable; recombinant; cloning; vehicles; provided; code; aspect; modified; host; cells; transformed; transfected; infected; andor; injected; vehicle; dna; provides; protein; comprising; systems; expression; amino acid; acid sequence; cloning vehicle; dna encoding; corresponding amino; /435/999/

Citation Formats

Croteau, Rodney B, Burke, Charles C, and Wildung, Mark R. Geranyl diphosphate synthase large subunit, and methods of use. United States: N. p., 2001. Web.
Croteau, Rodney B, Burke, Charles C, & Wildung, Mark R. Geranyl diphosphate synthase large subunit, and methods of use. United States.
Croteau, Rodney B, Burke, Charles C, and Wildung, Mark R. Mon . "Geranyl diphosphate synthase large subunit, and methods of use". United States. https://www.osti.gov/servlets/purl/874056.
@article{osti_874056,
title = {Geranyl diphosphate synthase large subunit, and methods of use},
author = {Croteau, Rodney B and Burke, Charles C and Wildung, Mark R},
abstractNote = {A cDNA encoding geranyl diphosphate synthase large subunit from peppermint has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Replicable recombinant cloning vehicles are provided which code for geranyl diphosphate synthase large subunit). In another aspect, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding geranyl diphosphate synthase large subunit. In yet another aspect, the present invention provides isolated, recombinant geranyl diphosphate synthase protein comprising an isolated, recombinant geranyl diphosphate synthase large subunit protein and an isolated, recombinant geranyl diphosphate synthase small subunit protein. Thus, systems and methods are provided for the recombinant expression of geranyl diphosphate synthase.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2001},
month = {Mon Jan 01 00:00:00 EST 2001}
}

Works referenced in this record:

Isolation of secretory cells from plant glandular trichomes and their use in biosynthetic studies of monoterpenes and other gland products
journal, January 1992