Title: Production of amino acids using auxotrophic mutants of methylotrophic bacillus

Abstract

A method of producing amino acids by culturing an amino acid auxotroph of a biologically pure strain of a type I methylotrophic bacterium of the genus Bacillus which exhibits sustained growth at 50.degree. C. using methanol as a carbon and energy source and requiring vitamin B.sub.12 and biotin is provided.

Inventors:

Hanson, Richard S ^[1]; Flickinger, Michael C ^[2]; Schendel, Frederick J ^[3]; Guettler, Michael V ^[4]

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+ Show Inventor Affiliations

1. Wayzata, MN
2. St. Paul, MN
3. Falcon Heights, MN
4. Waconia, MN

Issue Date:

Tue Jul 17 00:00:00 EDT 2001

Research Org.:

Univ. of Minnesota, Minneapolis, MN (United States)

OSTI Identifier:

873858

Patent Number(s):

6261825

Application Number:

07/673,264

Assignee:

Regents of University of Minnesota (Minneapolis, MN)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

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C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P13/04 - Alpha- or beta- amino acids {
C12P13/08 - Lysine
C12P13/20 - Aspartic acid

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

Y10S435/832 - Bacillus

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DOE Contract Number:  

AC02-82ER12029

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

production; amino; acids; auxotrophic; mutants; methylotrophic; bacillus; method; producing; culturing; acid; auxotroph; biologically; pure; strain; type; bacterium; genus; exhibits; sustained; growth; 50; degree; methanol; carbon; energy; source; requiring; vitamin; 12; biotin; provided; biologically pure; amino acid; energy source; amino acids; methylotrophic bacillus; /435/