Nucleic acid molecules encoding isopentenyl monophosphate kinase, and methods of use
Abstract
A cDNA encoding isopentenyl monophosphate kinase (IPK) from peppermint (Mentha x piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of isopentenyl monophosphate kinase (SEQ ID NO:2), from peppermint (Mentha x piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for isopentenyl monophosphate kinase, or for a base sequence sufficiently complementary to at least a portion of isopentenyl monophosphate kinase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding isopentenyl monophosphate kinase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant isopentenyl monophosphate kinase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant isopentenyl monophosphate kinase may be used to obtain expression or enhanced expression of isopentenyl monophosphate kinase in plants in order to enhance the production of isopentenyl monophosphate kinase, or isoprenoids derived therefrom, or may be otherwise employed for the regulation or expression of isopentenyl monophosphate kinase, or themore »
- Inventors:
-
- Pullman, WA
- Issue Date:
- Research Org.:
- Washington State University Research Foundation (Pullman, WA)
- OSTI Identifier:
- 873752
- Patent Number(s):
- 6235514
- Assignee:
- Washington State University Research Foundation (Pullman, WA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG03-96ER20212
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- nucleic; acid; molecules; encoding; isopentenyl; monophosphate; kinase; methods; cdna; ipk; peppermint; mentha; piperita; isolated; sequenced; corresponding; amino; sequence; determined; accordingly; dna; seq; provided; codes; expression; aspects; replicable; recombinant; cloning; vehicles; code; base; sufficiently; complementary; portion; rna; enable; hybridization; therewith; modified; host; cells; transformed; transfected; infected; injected; vehicle; systems; aforementioned; facilitate; production; isolation; purification; significant; amounts; obtain; enhanced; plants; enhance; isoprenoids; derived; therefrom; otherwise; employed; regulation; products; sequence sufficiently; acid molecule; modified host; cloning vehicles; recombinant expression; replicable recombinant; obtain expression; otherwise employed; enable hybridization; hybridization therewith; recombinant cloning; dna sequence; isolated dna; significant amounts; acid molecules; acid sequence; amino acid; nucleic acid; host cells; sequence encoding; base sequence; cloning vehicle; dna encoding; isopentenyl monophosphate; host cell; sufficiently complementary; monophosphate kinase; cdna encoding; derived therefrom; significant amount; corresponding amino; enhanced expression; aforementioned recombinant; encoding isopentenyl; /435/514/999/
Citation Formats
Croteau, Rodney B, and Lange, Bernd M. Nucleic acid molecules encoding isopentenyl monophosphate kinase, and methods of use. United States: N. p., 2001.
Web.
Croteau, Rodney B, & Lange, Bernd M. Nucleic acid molecules encoding isopentenyl monophosphate kinase, and methods of use. United States.
Croteau, Rodney B, and Lange, Bernd M. Mon .
"Nucleic acid molecules encoding isopentenyl monophosphate kinase, and methods of use". United States. https://www.osti.gov/servlets/purl/873752.
@article{osti_873752,
title = {Nucleic acid molecules encoding isopentenyl monophosphate kinase, and methods of use},
author = {Croteau, Rodney B and Lange, Bernd M},
abstractNote = {A cDNA encoding isopentenyl monophosphate kinase (IPK) from peppermint (Mentha x piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of isopentenyl monophosphate kinase (SEQ ID NO:2), from peppermint (Mentha x piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for isopentenyl monophosphate kinase, or for a base sequence sufficiently complementary to at least a portion of isopentenyl monophosphate kinase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding isopentenyl monophosphate kinase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant isopentenyl monophosphate kinase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant isopentenyl monophosphate kinase may be used to obtain expression or enhanced expression of isopentenyl monophosphate kinase in plants in order to enhance the production of isopentenyl monophosphate kinase, or isoprenoids derived therefrom, or may be otherwise employed for the regulation or expression of isopentenyl monophosphate kinase, or the production of its products.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2001},
month = {Mon Jan 01 00:00:00 EST 2001}
}
Works referenced in this record:
A family of transketolases that directs isoprenoid biosynthesis via a mevalonate-independent pathway
journal, March 1998
- Lange, B. M.; Wildung, M. R.; McCaskill, D.
- Proceedings of the National Academy of Sciences, Vol. 95, Issue 5
Cloning and characterization of a gene from Escherichia coli encoding a transketolase-like enzyme that catalyzes the synthesis of D-1-deoxyxylulose 5-phosphate, a common precursor for isoprenoid, thiamin, and pyridoxol biosynthesis
journal, March 1998
- Lois, L. M.; Campos, N.; Putra, S. R.
- Proceedings of the National Academy of Sciences, Vol. 95, Issue 5
Dedicated Roles of Plastid Transketolases during the Early Onset of Isoprenoid Biogenesis in Pepper Fruits 1
journal, August 1998
- Bouvier, Florence; d'Harlingue, Alain; Suire, Claude
- Plant Physiology, Vol. 117, Issue 4
Chromoplast development in ripening tomato fruit: identification of cDNAs for chromoplast-targeted proteins and characterization of a cDNA encoding a plastid-localized low-molecular-weight heat shock protein
journal, February 1997
- Lawrence, Susan D.; Cline, Kenneth; Moore, Gloria A.
- Plant Molecular Biology, Vol. 33, Issue 3, p. 483-492
Identification of a thiamin-dependent synthase in Escherichia coli required for the formation of the 1-deoxy-D-xylulose 5-phosphate precursor to isoprenoids, thiamin, and pyridoxol
journal, November 1997
- Sprenger, G. A.; Schorken, U.; Wiegert, T.
- Proceedings of the National Academy of Sciences, Vol. 94, Issue 24
Isopentenyl diphosphate biosynthesis via a mevalonate-independent pathway: Isopentenyl monophosphate kinase catalyzes the terminal enzymatic step
journal, November 1999
- Lange, B. M.; Croteau, R.
- Proceedings of the National Academy of Sciences, Vol. 96, Issue 24