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Title: Polyhydroxyalkanoate synthesis in plants

Abstract

Novel transgenic plants and plant cells are capable of biosynthesis of polyhydroxyalkanoate (PHA). Heterologous enzymes involved in PHA biosynthesis, particularly PHA polymerase, are targeted to the peroxisome of a transgenic plant. Transgenic plant materials that biosynthesize short chain length monomer PHAs in the absence of heterologous .beta.-ketothiolase and acetoacetyl-CoA reductase are also disclosed.

Inventors:
 [1];  [2];  [3];  [4]
  1. Lake Elmo, MN
  2. Roseville, MN
  3. New Brighton, MN
  4. Circle Pines, MN
Issue Date:
Research Org.:
The Consortium for Plant Biotechnology Research, Inc., West Lafayette, IN (US)
OSTI Identifier:
873164
Patent Number(s):
6103956
Assignee:
Regents of University of Minnesota (Minneapolis, MN)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FC05-92OR22072
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
polyhydroxyalkanoate; synthesis; plants; novel; transgenic; plant; cells; capable; biosynthesis; pha; heterologous; enzymes; involved; particularly; polymerase; targeted; peroxisome; materials; biosynthesize; chain; length; monomer; phas; absence; beta; -ketothiolase; acetoacetyl-coa; reductase; disclosed; pha polymerase; transgenic plants; plant cell; chain length; plant cells; transgenic plant; /800/435/

Citation Formats

Srienc, Friedrich, Somers, David A, Hahn, J J, and Eschenlauer, Arthur C. Polyhydroxyalkanoate synthesis in plants. United States: N. p., 2000. Web.
Srienc, Friedrich, Somers, David A, Hahn, J J, & Eschenlauer, Arthur C. Polyhydroxyalkanoate synthesis in plants. United States.
Srienc, Friedrich, Somers, David A, Hahn, J J, and Eschenlauer, Arthur C. Sat . "Polyhydroxyalkanoate synthesis in plants". United States. https://www.osti.gov/servlets/purl/873164.
@article{osti_873164,
title = {Polyhydroxyalkanoate synthesis in plants},
author = {Srienc, Friedrich and Somers, David A and Hahn, J J and Eschenlauer, Arthur C},
abstractNote = {Novel transgenic plants and plant cells are capable of biosynthesis of polyhydroxyalkanoate (PHA). Heterologous enzymes involved in PHA biosynthesis, particularly PHA polymerase, are targeted to the peroxisome of a transgenic plant. Transgenic plant materials that biosynthesize short chain length monomer PHAs in the absence of heterologous .beta.-ketothiolase and acetoacetyl-CoA reductase are also disclosed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2000},
month = {Sat Jan 01 00:00:00 EST 2000}
}

Works referenced in this record:

Stable transformation of maize after gene transfer by electroporation
journal, February 1986


Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
journal, August 1970


Production of Polyhydroxyalkanoates, a Family of Biodegradable Plastics and Elastomers, in Bacteria and Plants
journal, February 1995


Catalase
book, January 1974


Polyhydroxyalkanoic acids
book, January 1991


Expression of genes transferred into monocot and dicot plant cells by electroporation.
journal, September 1985


An NADP-linked acetoacetyl CoA reductase from Zoogloea ramigera
journal, January 1977


Agrobacterium-Mediated Plant Transformation and its Further Applications to Plant Biology
journal, June 1987


A Pseudomonas strain accumulating polyesters of 3-hydroxybutyric acid and medium-chain-length 3-hydroxyalkanoic acids
journal, September 1992


Purification and properties of ?-ketothiolase from Zoogloea ramigera
journal, January 1978


Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation.
journal, December 1994


Metabolism in Plant Peroxisomes
book, January 1982


Poly(β-hydroxyalkanoate) copolymers containing brominated repeating units produced by Pseudomonas oleovorans
journal, March 1992


Modification of Plant Lipid Synthesis
journal, May 1995


Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants
journal, September 1990


Manipulation of the genes for poly-?-hydroxybutyric acid synthesis in Alcaligenes eutrophus
journal, July 1995


Microbodies in Higher Plants
journal, June 1979


Biogenesis of Peroxisomes
journal, November 1985


One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution.
journal, April 1989


A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures
journal, July 1962


Genetically transformed maize plants from protoplasts
journal, April 1988


Polyhydroxybutyrate, a Biodegradable Thermoplastic, Produced in Transgenic Plants
journal, April 1992


Bacteriophage lambda cloning system for the construction of directional cDNA libraries.
journal, June 1987


Plant Organellar Targeting Sequences
book, January 1993


Poly-β-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16
journal, September 1989


Production of heteropolymeric polyhydroxyalkanoate in Eschericha coli from a single carbon source
journal, August 1996


Intracellular pH in singleSaccharomyces cerevisiae cells
journal, March 1989