Reversible gelling culture media for in-vitro cell culture in three-dimensional matrices
Abstract
A gelling cell culture medium useful for forming a three dimensional matrix for cell culture in vitro is prepared by copolymerizing an acrylamide derivative with a hydrophilic comonomer to form a reversible (preferably thermally reversible) gelling linear random copolymer in the form of a plurality of linear chains having a plurality of molecular weights greater than or equal to a minimum gelling molecular weight cutoff, mixing the copolymer with an aqueous solvent to form a reversible gelling solution and adding a cell culture medium to the gelling solution to form the gelling cell culture medium. Cells such as chondrocytes or hepatocytes are added to the culture medium to form a seeded culture medium, and temperature of the medium is raised to gel the seeded culture medium and form a three dimensional matrix containing the cells. After propagating the cells in the matrix, the cells may be recovered by lowering the temperature to dissolve the matrix and centrifuging.
- Inventors:
-
- Charleston, SC
- Mt. Pleasant, SC
- Richland, WA
- Issue Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- OSTI Identifier:
- 873161
- Patent Number(s):
- 6103528
- Assignee:
- Battelle Memorial Institute (Richland, WA); Medical University of South Carolina (Charleston, SC)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC06-76RL01830
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- reversible; gelling; culture; media; in-vitro; cell; three-dimensional; matrices; medium; useful; forming; dimensional; matrix; vitro; prepared; copolymerizing; acrylamide; derivative; hydrophilic; comonomer; form; preferably; thermally; linear; random; copolymer; plurality; chains; molecular; weights; equal; minimum; weight; cutoff; mixing; aqueous; solvent; solution; adding; cells; chondrocytes; hepatocytes; added; seeded; temperature; raised; containing; propagating; recovered; lowering; dissolve; centrifuging; reversible gelling; random copolymer; aqueous solvent; culture medium; molecular weight; cell culture; molecular weights; thermally reversible; dimensional matrix; matrix containing; linear random; vitro cell; weight cu; acrylamide derivative; /435/424/
Citation Formats
An, Yuehuei H, Mironov, Vladimir A, and Gutowska, Anna. Reversible gelling culture media for in-vitro cell culture in three-dimensional matrices. United States: N. p., 2000.
Web.
An, Yuehuei H, Mironov, Vladimir A, & Gutowska, Anna. Reversible gelling culture media for in-vitro cell culture in three-dimensional matrices. United States.
An, Yuehuei H, Mironov, Vladimir A, and Gutowska, Anna. Sat .
"Reversible gelling culture media for in-vitro cell culture in three-dimensional matrices". United States. https://www.osti.gov/servlets/purl/873161.
@article{osti_873161,
title = {Reversible gelling culture media for in-vitro cell culture in three-dimensional matrices},
author = {An, Yuehuei H and Mironov, Vladimir A and Gutowska, Anna},
abstractNote = {A gelling cell culture medium useful for forming a three dimensional matrix for cell culture in vitro is prepared by copolymerizing an acrylamide derivative with a hydrophilic comonomer to form a reversible (preferably thermally reversible) gelling linear random copolymer in the form of a plurality of linear chains having a plurality of molecular weights greater than or equal to a minimum gelling molecular weight cutoff, mixing the copolymer with an aqueous solvent to form a reversible gelling solution and adding a cell culture medium to the gelling solution to form the gelling cell culture medium. Cells such as chondrocytes or hepatocytes are added to the culture medium to form a seeded culture medium, and temperature of the medium is raised to gel the seeded culture medium and form a three dimensional matrix containing the cells. After propagating the cells in the matrix, the cells may be recovered by lowering the temperature to dissolve the matrix and centrifuging.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2000},
month = {Sat Jan 01 00:00:00 EST 2000}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Thermally reversible polymer gels for biohybrid artificial pancreas
journal, May 1996
- Vernon, Brent; Gutowska, Anna; Wan Kim, Sung
- Macromolecular Symposia, Vol. 109, Issue 1
Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels
journal, August 1982
- Benya, P.
- Cell, Vol. 30, Issue 1
Fibroblast growth factor stimulates colony formation of differentiated chondrocytes in soft agar
journal, December 1987
- Kato, Yukio; Iwamoto, Masahiro; Koike, Tatsuya
- Journal of Cellular Physiology, Vol. 133, Issue 3
Treatment of Deep Cartilage Defects in the Knee with Autologous Chondrocyte Transplantation
journal, October 1994
- Brittberg, Mats; Lindahl, Anders; Nilsson, Anders
- New England Journal of Medicine, Vol. 331, Issue 14
Develoapmet of a Tissue Analog for Cartilage Repair
journal, January 1991
- Pachence, J. M.; Frenkel, S. R.; Lin, H.
- MRS Proceedings, Vol. 252
Enhanced Tumor Growth of Both Primary and Established Human and Murine Tumor Cells in Athymic Mice After Coinjection With Matrigel
journal, June 1991
- Fridman, R.; Kibbey, M. C.; Royce, L. S.
- JNCI Journal of the National Cancer Institute, Vol. 83, Issue 11