Reducing nontemplated 3' nucleotide addition to polynucleotide transcripts
Abstract
Non-template 3' nucleotide addition to a transcript is reduced by transcribing a transcript from a template comprising an ultimate and/or penultimate 5' ribose having a C'2 substituent such as methoxy, which reduces non-template 3' nucleotide addition to the transcript. The methods are shown to be applicable to a wide variety of polymerases, including Taq, T7 RNA polymerase, etc.
- Inventors:
-
- Bloomington, IN
- Issue Date:
- Research Org.:
- Univ. of California (United States)
- OSTI Identifier:
- 873102
- Patent Number(s):
- 6090590
- Assignee:
- Regents of University of California (Oakland, CA); Advanced Research and Technology, Inc. (Indianapolis, IN)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FG03-86ER60406
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- reducing; nontemplated; nucleotide; addition; polynucleotide; transcripts; non-template; transcript; reduced; transcribing; template; comprising; ultimate; penultimate; ribose; substituent; methoxy; reduces; methods; shown; applicable; wide; variety; polymerases; including; taq; t7; rna; polymerase; etc; rna polymerase; t7 rna; wide variety; nucleotide addition; rna poly; /435/999/
Citation Formats
Kao, C Cheng. Reducing nontemplated 3' nucleotide addition to polynucleotide transcripts. United States: N. p., 2000.
Web.
Kao, C Cheng. Reducing nontemplated 3' nucleotide addition to polynucleotide transcripts. United States.
Kao, C Cheng. Sat .
"Reducing nontemplated 3' nucleotide addition to polynucleotide transcripts". United States. https://www.osti.gov/servlets/purl/873102.
@article{osti_873102,
title = {Reducing nontemplated 3' nucleotide addition to polynucleotide transcripts},
author = {Kao, C Cheng},
abstractNote = {Non-template 3' nucleotide addition to a transcript is reduced by transcribing a transcript from a template comprising an ultimate and/or penultimate 5' ribose having a C'2 substituent such as methoxy, which reduces non-template 3' nucleotide addition to the transcript. The methods are shown to be applicable to a wide variety of polymerases, including Taq, T7 RNA polymerase, etc.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2000},
month = {Sat Jan 01 00:00:00 EST 2000}
}
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